Objective:Observation of ginger (Zingiber Officinale Sucus, ZOS) effect on rabbit isolated duodenum and to explore the correlation mechanism with M and H1 receptor. To investigate the effects of ZOS on isolated duoden...Objective:Observation of ginger (Zingiber Officinale Sucus, ZOS) effect on rabbit isolated duodenum and to explore the correlation mechanism with M and H1 receptor. To investigate the effects of ZOS on isolated duodenum of rabbits treated with acetylcholine, histamine, atropine and chlorpheniramine respectively. Methods: The optimal concentration of ZOS was 1% and the above drugs were applied to the isolated duodenal segments respectively. The contractile force was recorded by BL-420E biological signal acquisition system. Results: The results showed that ZOS significantly inhibited the contraction of isolated rabbit duodenal smooth muscle in a positive concentration-dependent manner. The mechanism of inhibiting contraction had little correlation with M and H1 receptors. Conclusion: It could inhibit the efficacy of acetylcholine and histamine, and was a synergist for atropine and chlorpheniramine. This study provided a basis for the use of ginger in daily life and the development of combined medication.展开更多
Objective This study aims to establish and evaluate the methodology of isolated rabbit eye （IRE） test. Methods IRE test was performed according to modifications of the in vitro toxicology （INVITTOX） Protocol No.85...Objective This study aims to establish and evaluate the methodology of isolated rabbit eye （IRE） test. Methods IRE test was performed according to modifications of the in vitro toxicology （INVITTOX） Protocol No.85： Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods （ECVAM）, and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests. A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test. Results IRE test was established successfully in our laboratory. It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals （Fisher＇s Exact Test X2=51.314, P〈0.001; McNemar P=0.261; Gamma=0.960, P〈0.001; Kappa=0.843, P〈0.001） and 26 cosmetic products （Fisher＇s Exact Test X^2=15.522, P〈0.O01; McNemar P=0.311; Gamma=0.967, P〈0.001; Kappa=0.611, P〈0.O01）. Conclusion IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test.展开更多
Objective Levosimendan,a new calcium ion sensitizer,is currently used in the treatment of heart failure and as an option for patients with injury to the left heart or at high risk for surgery. The study tried to evalu...Objective Levosimendan,a new calcium ion sensitizer,is currently used in the treatment of heart failure and as an option for patients with injury to the left heart or at high risk for surgery. The study tried to evaluate the effects of levosimendan and ulinastain for protecting myocardium from ischemia-reperfusion (I / R) injury to展开更多
Dermatophyte infection or ringworm is a superficial cutaneous infection with one or more of the fungal species of the keratinophilic genera Microsporum, Trichophyton, or Epidermophyton and is a zoonosis with a great i...Dermatophyte infection or ringworm is a superficial cutaneous infection with one or more of the fungal species of the keratinophilic genera Microsporum, Trichophyton, or Epidermophyton and is a zoonosis with a great impact on public health. Dermatophytes were identified from rabbit sample cultures submitted to mycological examination in the Laboratory of Microbiology of the University of Tr^-os-Montes e Alto Douro, Vila Real, Portugal. All samples were collected from suspected clinical cases. Dermatophytes were cultured from 4 of the 55 specimens （7.3%）. The dermatophytes isolated were Trichophyton mentagrophytes var. mentagrophytes （1.8%） and Microsporum gypseum （5.5%）. Microscopic examination was negative in all specimens. In this work, Scopulariopsis spp., a contaminant mould, was identified in 13 specimens （23.6%）. The proportion of positive samples in relation to the number of samples examined from cases suspected was very low. As all samples were collected from rabbits with compatible signs, we presume that the low prevalence of isolation was due to laboratory constraints on dermatophytes diagnosis.展开更多
针对离体兔眼(IRE)试验只适用于筛选严重眼刺激性(UN GHS第1类)的缺陷,建立IRE试验结合基于细胞活性生物标记物的荧光染色方法用于评价化妆品眼刺激性。通过IRE试验条件的探索,包括测试浓度和后暴露时间,对市售化妆品进行眼刺激性检测,...针对离体兔眼(IRE)试验只适用于筛选严重眼刺激性(UN GHS第1类)的缺陷,建立IRE试验结合基于细胞活性生物标记物的荧光染色方法用于评价化妆品眼刺激性。通过IRE试验条件的探索,包括测试浓度和后暴露时间,对市售化妆品进行眼刺激性检测,同时对角膜进行冰冻切片并染色,鬼笔环肽对丝状肌动蛋白进行染色,DAPI染色胞核后,测量损伤深度(depth of injury,DOI),并比较体外DOI、体内DOI及Draize试验结果,建立预测模型。结果显示通过DOI检测,可对化妆品无刺激性至中刺激性进行检测,表明在离体兔眼中,基于细胞活力的DOI测量可作为角膜损伤的生物标志物,适用于化妆品眼刺激性的评价。展开更多
Crohn's disease(CD) can involve any part of the gastrointestinal tract from the mouth to anus. However, gastroduodenal CD is rare with a frequency reported to range between 0.5% and 4.0%. Most patients with gastro...Crohn's disease(CD) can involve any part of the gastrointestinal tract from the mouth to anus. However, gastroduodenal CD is rare with a frequency reported to range between 0.5% and 4.0%. Most patients with gastroduodenal CD have concomitant lesions in the terminal ileum or colon, but isolated gastroduodenal Crohn's disease is an extremely rare presentation of the disease accounting for less than 0.07% of all patients with CD. The symptoms of gastroduodenal CD include epigastric pain, dyspepsia, early satiety, anorexia, nausea, vomiting, and weight loss. The diagnosis of gastroduodenal CD requires a high level of clinical suspicion and can be made by comprehensive clinical evaluation. Here we report a rare case of isolated duodenal CD not confirmed by identification of granuloma on biopsy, but diagnosed by clinical evaluation.展开更多
As the first step of selecting a specific mRNA,we would have polyribosomcs available.Weused ultracentrifugation method to isolate poly-ribosomes from rat liver.The UV spectrum andA260/A280 ratio of this preparation sh...As the first step of selecting a specific mRNA,we would have polyribosomcs available.Weused ultracentrifugation method to isolate poly-ribosomes from rat liver.The UV spectrum andA260/A280 ratio of this preparation showed thecharacteristics of polyribosomes,so did the sedi-展开更多
970337 The effects teprenone on the ulcer healingrate and the quality of healing in the treatment of ac-tive gastric ulcers. HU Fulian(胡伏莲), et al. DeptGastroenterol, 1st Teach Hosp, Beijing Med Univ, Bei-jing, 100...970337 The effects teprenone on the ulcer healingrate and the quality of healing in the treatment of ac-tive gastric ulcers. HU Fulian(胡伏莲), et al. DeptGastroenterol, 1st Teach Hosp, Beijing Med Univ, Bei-jing, 100034 Chin J Intern Med 1996; 35(12): 807-809. Objective: To explore the effects of teprenone on展开更多
基金Undergraduate Innovation and Entrepreneurship Project of Hainan Medical University in 2019(No.201911810013).
文摘Objective:Observation of ginger (Zingiber Officinale Sucus, ZOS) effect on rabbit isolated duodenum and to explore the correlation mechanism with M and H1 receptor. To investigate the effects of ZOS on isolated duodenum of rabbits treated with acetylcholine, histamine, atropine and chlorpheniramine respectively. Methods: The optimal concentration of ZOS was 1% and the above drugs were applied to the isolated duodenal segments respectively. The contractile force was recorded by BL-420E biological signal acquisition system. Results: The results showed that ZOS significantly inhibited the contraction of isolated rabbit duodenal smooth muscle in a positive concentration-dependent manner. The mechanism of inhibiting contraction had little correlation with M and H1 receptors. Conclusion: It could inhibit the efficacy of acetylcholine and histamine, and was a synergist for atropine and chlorpheniramine. This study provided a basis for the use of ginger in daily life and the development of combined medication.
基金supported by a grant from the Ministry of Health of the People's Republic of China for the Alternative to animals Testing project,200802080
文摘Objective This study aims to establish and evaluate the methodology of isolated rabbit eye （IRE） test. Methods IRE test was performed according to modifications of the in vitro toxicology （INVITTOX） Protocol No.85： Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods （ECVAM）, and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests. A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test. Results IRE test was established successfully in our laboratory. It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals （Fisher＇s Exact Test X2=51.314, P〈0.001; McNemar P=0.261; Gamma=0.960, P〈0.001; Kappa=0.843, P〈0.001） and 26 cosmetic products （Fisher＇s Exact Test X^2=15.522, P〈0.O01; McNemar P=0.311; Gamma=0.967, P〈0.001; Kappa=0.611, P〈0.O01）. Conclusion IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test.
文摘Objective Levosimendan,a new calcium ion sensitizer,is currently used in the treatment of heart failure and as an option for patients with injury to the left heart or at high risk for surgery. The study tried to evaluate the effects of levosimendan and ulinastain for protecting myocardium from ischemia-reperfusion (I / R) injury to
文摘Dermatophyte infection or ringworm is a superficial cutaneous infection with one or more of the fungal species of the keratinophilic genera Microsporum, Trichophyton, or Epidermophyton and is a zoonosis with a great impact on public health. Dermatophytes were identified from rabbit sample cultures submitted to mycological examination in the Laboratory of Microbiology of the University of Tr^-os-Montes e Alto Douro, Vila Real, Portugal. All samples were collected from suspected clinical cases. Dermatophytes were cultured from 4 of the 55 specimens （7.3%）. The dermatophytes isolated were Trichophyton mentagrophytes var. mentagrophytes （1.8%） and Microsporum gypseum （5.5%）. Microscopic examination was negative in all specimens. In this work, Scopulariopsis spp., a contaminant mould, was identified in 13 specimens （23.6%）. The proportion of positive samples in relation to the number of samples examined from cases suspected was very low. As all samples were collected from rabbits with compatible signs, we presume that the low prevalence of isolation was due to laboratory constraints on dermatophytes diagnosis.
文摘针对离体兔眼(IRE)试验只适用于筛选严重眼刺激性(UN GHS第1类)的缺陷,建立IRE试验结合基于细胞活性生物标记物的荧光染色方法用于评价化妆品眼刺激性。通过IRE试验条件的探索,包括测试浓度和后暴露时间,对市售化妆品进行眼刺激性检测,同时对角膜进行冰冻切片并染色,鬼笔环肽对丝状肌动蛋白进行染色,DAPI染色胞核后,测量损伤深度(depth of injury,DOI),并比较体外DOI、体内DOI及Draize试验结果,建立预测模型。结果显示通过DOI检测,可对化妆品无刺激性至中刺激性进行检测,表明在离体兔眼中,基于细胞活力的DOI测量可作为角膜损伤的生物标志物,适用于化妆品眼刺激性的评价。
文摘Crohn's disease(CD) can involve any part of the gastrointestinal tract from the mouth to anus. However, gastroduodenal CD is rare with a frequency reported to range between 0.5% and 4.0%. Most patients with gastroduodenal CD have concomitant lesions in the terminal ileum or colon, but isolated gastroduodenal Crohn's disease is an extremely rare presentation of the disease accounting for less than 0.07% of all patients with CD. The symptoms of gastroduodenal CD include epigastric pain, dyspepsia, early satiety, anorexia, nausea, vomiting, and weight loss. The diagnosis of gastroduodenal CD requires a high level of clinical suspicion and can be made by comprehensive clinical evaluation. Here we report a rare case of isolated duodenal CD not confirmed by identification of granuloma on biopsy, but diagnosed by clinical evaluation.
文摘As the first step of selecting a specific mRNA,we would have polyribosomcs available.Weused ultracentrifugation method to isolate poly-ribosomes from rat liver.The UV spectrum andA260/A280 ratio of this preparation showed thecharacteristics of polyribosomes,so did the sedi-
文摘970337 The effects teprenone on the ulcer healingrate and the quality of healing in the treatment of ac-tive gastric ulcers. HU Fulian(胡伏莲), et al. DeptGastroenterol, 1st Teach Hosp, Beijing Med Univ, Bei-jing, 100034 Chin J Intern Med 1996; 35(12): 807-809. Objective: To explore the effects of teprenone on
