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Changes of Estrogen in Serum and Estrogen Receptor β in the Relevant Brain Regions Following Mating Behavior of the Male Mandarin Vole Microtus mandarinus 被引量:2
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作者 何凤琴 张巨武 +1 位作者 石靖 王波 《Zoological Research》 CAS CSCD 北大核心 2008年第5期529-536,共8页
In order to investigate the estrogen and estrogen receptor β changes after mating behavior of male mandarin vole (Microtus mandarinus), the radioimmunoassay (RIA) and immunohistochemistry methods were used to inv... In order to investigate the estrogen and estrogen receptor β changes after mating behavior of male mandarin vole (Microtus mandarinus), the radioimmunoassay (RIA) and immunohistochemistry methods were used to investigate changes of the serum estrogen (E) concentrations, estrogen immunoreactive neurons (E-IRs) and estrogen receptor β immunoreactive neurons (ERβ-IRs) in the relevant brain regions following mating behavior. Fifteen sexually matured male voles were randomly divided into three groups and treated differently: (1) control group: voles were exposed to clean hard-wood shavings (n=5), (2) exposure group: voles were exposed to the soiled bedding for more than 24h on which estrous females had been placed (n=5), and (3) mating group: voles were placed with an estrous female for more than 24h (n=5). The results showed circulating serum E concentrations were significantly higher in the mating group than in the exposure group and the control group, and there were no significant difference between the exposure group and the control group. E-IRs and ERβ-IRs were detected in the following brain regions related to mating behavior: the arcuate nucleus (ARC), bed nucleus of the stria terminalis (BST), lateral septal nucleus (LS), medial amygdaloid nucleus (ME), medial preoptic area (MPO) and ventromedial hypothalamic nucleus (VMH). The results showed that there were significantly more E-IRs in the six brain regions in the mating group than in the control group and the exposure group, and there were no significant difference between the exposure group and the control group except for LS. There was no significant difference in ERβ-IRs in the six brain regions among the three groups, and there were some lighter -stained ERβ-IRs in these brain regions. The results suggested that estrogen affect mating activity of male mandarin voles, but ERβ might not play an important role in mating behavior of male mandarin voles. Instead, it might be through other receptors. 展开更多
关键词 Mandarin voles (Microtus mandarinus): Estrogen Estrogen receptor β RADIOIMMUNOASSAY Mating behavior
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Effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and central secretory tissues 被引量:31
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作者 JiLin Guang-TaoYan Xiu-HuaHao Lu-HuanWang KaiZhang HuiXue 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第7期1000-1004,共5页
AIM: To explore the effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and their central secretory tissues and to find out the role leptin and orexin-A play i... AIM: To explore the effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and their central secretory tissues and to find out the role leptin and orexin-A play in acute inflammatory responses.METHODS: An intestinal ischemia-reperfusion (I/R)injury model of rats was established and rats were divided randomly into six groups: sham-operation group, 60 min ischemia/30 min reperfusion group (I60'R30'), I60'R90',I60'R150', I60'R240' and I60'R360', 9 rats each group.Two highly-sensitive radioimmunoassays for leptin and orexin-A were established and used to check the change of their concentrations in peripheral blood and central secretory tissues before and after intestinal I/R injury.RESULTS: Compared with the serum leptin level before injury, it decreased significantly in I60'R30' group and increased significantly in I60'R360' group; compared to sham-operation group after injury, serum leptin level increased significantly in I60'R360' group; compared to sham-operation group after injury, adipose leptin levels decreased significantly in I60'R30' and I60'R90' groups,while increased significantly in I60'R360' group. There was no significant difference between the expression levels of orexin-A before and after I/R injury.CONCLUSION: Leptin has a time-dependent response and orexin-A has a delayed response to acute inflammatory stimuli such as intestinal I/R injury and they may participate in metabolic disorders in injury as inflammatory cytokines. 展开更多
关键词 ISCHEMIA-REPERFUSION Intestinal LEPTIN OrexinA RADIOIMMUNOASSAY Inflammation Acute Cytokine
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Plasma endothelin in patients with endotoxemia and dynamic comparison between vasoconstrictor and vasodilator in cirrhotic patients 被引量:8
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作者 Feng Liu1 Ji Xin Li2, Chun Mei Li2 Xi Sheng Leng2 1Department of General Surgery, the Fifth Affiliated Hospital, Harbin Medical University, Harbin 150036, Heilongjiang Province, China2Department of General Surgery, People’s Hospital, Medical University, Beijing 100044, China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第1期126-127,共2页
INTRODUCTIONPortal hypertension is a common clinical syndromecharacterized by an abnormal increase in portalblood to the systemic circulation, bypassing theliver. Recent studies have reported that humoralsubstances pl... INTRODUCTIONPortal hypertension is a common clinical syndromecharacterized by an abnormal increase in portalblood to the systemic circulation, bypassing theliver. Recent studies have reported that humoralsubstances play an important role in thepathogenesis of portal hypertension, either byincreasing vascular resistance at both theintrahepatic and porto-collateral sites or affectingsplanchnic vasodilation with a concomitant increasein parto-collateral blood flow[1-6] 展开更多
关键词 hypertension portal LIVER CIRRHOSIS portosystemic shunt surgical ENDOTHELINS radioimmunoassay EPOPROSTENOL LIVER CIRRHOSIS
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Differences and significance of motilin,vasoactive intestinal peptide and gastrin in blood and gallbladder tissues of patients with gallstones 被引量:8
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作者 Zhang, Zhen-Hai Wu, Shuo-Dong +4 位作者 Su, Yang Jin, Jun-Zhe Fan, Ying Yu, Hong Zhang, Li-Kui 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2008年第1期58-64,共7页
BACKGROUND: The disorders of gallbladder motility may play an important role in the formation of gallstones. Many neural and hormonal factors and their interactions regulate gallbladder motility and bile flow into the... BACKGROUND: The disorders of gallbladder motility may play an important role in the formation of gallstones. Many neural and hormonal factors and their interactions regulate gallbladder motility and bile flow into the duodenum. Further study in these factors may help to reveal the etiology of gallbladder diseases. This study was undertaken to assess the relationship of the levels of motilin, vasoactive intestinal peptide (VIP) and gastrin in blood and gallbladder tissues with the formation of cholelithiasis. METHODS: The levels of motilin, gastrin and VIP in blood and gallbladder tissues of 36 patients with gallbladder stones, 14 patients with gallbladder polyps, 10 healthy volunteers and 10 patients with common bile duct stones were measured by radioimmunoassay. RESULTS: The level of motilin in plasma and gallbladder tissues of the gallbladder stone group was higher than that of the control and gallbladder polyp groups (P<0.05). The levels of plasma VIP and serum gastrin were much higher than those of the other three groups (P<0.01). The level of VIP in gallbladder tissues was higher than that of the control and gallbladder polyp groups (P<0.01). CONCLUSIONS: The abnormal excretion of hormonal factors is closely related to gallstone formation. The high level of VIP in gallbladder tissues may be an important cause of gallbladder hypomotility. The abnormal level of serum gastrin may be related to the gastrointestinal symptoms of patients with gallstones. 展开更多
关键词 MOTILIN vasoactive intestinal peptide GASTRIN GALLBLADDER RADIOIMMUNOASSAY
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CEA and AFP expression in human hepatoma cells transfected with antisense IGF-I gene 被引量:7
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作者 ZHANG Li LI Shu-Nong WANG Xiao-Ning 《World Journal of Gastroenterology》 SCIE CAS CSCD 1998年第1期35-37,共3页
IM To determine whether antisense insulinlike growth factorI(IGFI) gene can modulate CEA and AFP expression in human hepatoma cells (HepG2).METHODS Transfection of HepG2 cells was accomplished using Lipofectin reage... IM To determine whether antisense insulinlike growth factorI(IGFI) gene can modulate CEA and AFP expression in human hepatoma cells (HepG2).METHODS Transfection of HepG2 cells was accomplished using Lipofectin reagent. Northern blot analysis confirmed the antisense IGFI RNA of the transfected cells. CEA and AFP levels were measured using radioimmunoassay.RESULTS Human hepatoma cell lines (HepG2) were transfected with antisense IGFI gene. Northern blot analysis confirmed that antisense IGFI RNA was expressed in the transfected cells. The effect of antisense IGFI gene on CEA and AFP expression was demonstrated by the fact that the CEA and AFP levels in the supernatant of transfected cell culture were significantly lower as compared with the parent cells, 〔CEA 70μg/L±076μg/L and 329μg/L±180μg/L (P<005) and AFP 5363μg/L±602μg/L and 90μg/L±526μg/L (P<001), respectively〕.CONCLUSION The malignant potentiality of the transfected cells was partially suppressed. Antisense IGFI gene can modulate the expression of CEA and AFP in human hepatoma cell lines (HepG2). 展开更多
关键词 insulinlike growth factor-I carcinoembryonic antigen αfetoprotein carcinoma hepatocellular tumor cells cultured RADIOIMMUNOASSAY
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Expressions of ICAM-1 and its mRNA in sera and tissues of patients with hepatocellular carcinoma 被引量:14
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作者 Jing Xu1 Ming Hui Mei1 +3 位作者 Si En Zeng2 Qing Fen Shi3 Yong Ming Liu4 Li Ling Qin3 1Department of Hepatobiliary Surgery2Department of Pathology3Institute of Hepatobiliary Surgery4Department of Biochemistry, Guilin 541001, Guangxi Province, China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第1期120-125,共6页
INTRODUCTIONThe increased expression of ICAM-1 on a widerange of cells and in the sera of patients withmalignancies, chronic liver diseases andinflammation diseases has been described since thelate 1980s[1-22]. Recent... INTRODUCTIONThe increased expression of ICAM-1 on a widerange of cells and in the sera of patients withmalignancies, chronic liver diseases andinflammation diseases has been described since thelate 1980s[1-22]. Recently rapid progress in studieson expression of ICAM-1 in patients withhepatocellular carcinoma ( HCC ) have beenachieved, including clinical and experimentalresearches[23-31]. 展开更多
关键词 Carcinoma Hepatocellular Follow-Up Studies Humans IMMUNOHISTOCHEMISTRY Intercellular Adhesion Molecule-1 Liver Liver Neoplasms Predictive Value of Tests Prognosis RNA Messenger RADIOIMMUNOASSAY Research Support Non-U.S. Gov't Reverse Transcriptase Polymerase Chain Reaction Solubility ALPHA-FETOPROTEINS
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Increased nociceptin/orphanin FQ plasma levels in hepatocellular carcinoma 被引量:5
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作者 FerencSzalay AndreaHorvath +5 位作者 PeterL.Lakatos AnikoFolhoffer KingaDunkel DalmaHegedus KornéliaTekes MónikaBHantos 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第1期42-45,共4页
AIM:The heptadecapeptide nociceptin alias orphanin FQ is the endogenous agonist of opioid receptor-likel receptor. It is involved in modulation of pain and cognition.High blood level was reported in patients with acut... AIM:The heptadecapeptide nociceptin alias orphanin FQ is the endogenous agonist of opioid receptor-likel receptor. It is involved in modulation of pain and cognition.High blood level was reported in patients with acute and chronic pain, and in Wilson disease.An accidental observation led us to investigate nociceptin in hepatocellular carcinoma. METHODS:Plasma nociceptin level was measured by radioimmunoassay,aprotinin was used as protease inhibitor. Hepatocellular carcinoma was diagnosed by laboratory, ultrasound,other imaging,and confirmed by fine needle biopsy.Results were compared to healthy controls and patients with other chronic liver diseases. RESULTS:Although nociceptin levels were elevated in patients with Wilson disease (14.0±2.7 pg/mL,n=26), primary biliary cirrhosis (12.1±3.2 pg/mL,n=21) and liver cirrhosis (12.8±4.0 pg/mL,n=15) compared to the healthy controls (9.2±1.8 pg/mL,n=29, P<0.001 for each),in patients with hepatocellular carcinoma a ten-fold increase was found (105.9±14.4 pg/mL,n=29,P<0.0001).High plasma levels were found in each hepatocellular carcinoma patient including those with normal alpha fetoprotein and those with pain (104.9±14.9 pg/mL,n=12) and without (107.7±14.5 pg/mL,n=6). CONCLUSION:A very high nociceptin plasma level seems to be an indicator for hepatocellular carcinoma.Further research is needed to clarify the mechanism and clinical significance of this novel finding. 展开更多
关键词 ADULT Aged Aged 80 and over Carcinoma Hepatocellular FEMALE Humans Liver Cirrhosis Liver Neoplasms Male Middle Aged Opioid Peptides RADIOIMMUNOASSAY
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Effects of morphine and electroacupuncture on substance P level in spinal cord and their relation to pain threshold in rats 被引量:2
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作者 阮怀珍 李希成 黎海蒂 《Journal of Medical Colleges of PLA(China)》 CAS 1993年第1期79-83,共5页
Immunoreactive substance P(Ir-SP)level,and substance P likeimmunoreactivity(SP-Li)in the spinal cord were observed with radioimmunoassay andimmunohistochemistry in rats after they were given an intraperitoneal injecti... Immunoreactive substance P(Ir-SP)level,and substance P likeimmunoreactivity(SP-Li)in the spinal cord were observed with radioimmunoassay andimmunohistochemistry in rats after they were given an intraperitoneal injection of mor-phine(7.5mg/kg)or electroacupunctured(3V and 3Hz)on the “Jiaji point”.It wasfound that the pain threshold(PT),Ir-SP level and SP-Li in the dorsal horn of the spi-nal cord were more significantly increased in the animals after the administration ofmorphine or electroacupuncture than in the control(P【0.05~0.01).The combined effectsof morphine and electropacupuncture were even more powerful than either of the agentswas administered singly.Naloxone could block the analgesic effect and the elevation ofIr-SP due to morphine or electroacupuncture.The findings suggest that there is a synergismbe tween morphine and electroacupuncture and the analgesic effect of the 2 depends uponthe increase of Ir-SP level of the spinal cord mediated through the opiate receptors. 展开更多
关键词 MORPHINE ELECTROACUPUNCTURE spinal CORD substance P RADIOIMMUNOASSAY immunohistochemistry pain threshold animal RATS
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Detection of Laminin in Serum and Ascites from Patients with Epithelial Ovarian Tumor 被引量:2
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作者 初永丽 杨元先 +1 位作者 林美华 王泽华 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2002年第1期58-59,68,共3页
The change in serum laminin (LN) level and its clinical significance in epithelial ovarian tumor were investigated. The LN levels in serum and ascites samples from 69 patients with epithelial ovarian tumor and 42 case... The change in serum laminin (LN) level and its clinical significance in epithelial ovarian tumor were investigated. The LN levels in serum and ascites samples from 69 patients with epithelial ovarian tumor and 42 cases as control group before and after operation were analyzed by radioimmunoassay. The results showed that the serum LN levels in the patients with malignant tumors (157.85 ±14.37 ng/ml) were significantly higher than that in the control group (125.14 ±7.03 ng/ml) and in the patients with benign tumors (128.36±8.75 ng/ml)(both P <0.01) before operation. The serum LN levels in the malignant group were decreased significantly after operation as compared with those before operation ( P <0.05). The serum LN levels in low differentiated tumors was higher than those in moderate differentiated tumors and high differentiated tumors ( P <0.05). The LN levels in ascites (172.94±15.26 ng/ml) was significantly higher than in serum (161.34±6.59 ng/ml)( P <0.05) in malignant tumors. The serum LN levels in the patients with lymph node metastasis (165.41±19.91 ng/ml) was obviously higher than those without lymph node metastasis (152.35±10.34 ng/ml)( P <0.05). It was concluded that LN levels in serum and acistes were remarkably increased in malignant epithelial ovarian tumors, suggesting that LN might be one of important diameters reflecting tumor biological characteristics. 展开更多
关键词 ovarian tumor LAMININ RADIOIMMUNOASSAY
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Radioimmunoassay of Antiarrhythmic Peptide and Its application 被引量:1
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作者 吴志坚 张永学 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2003年第1期44-47,共4页
To establish a specific and sensitive radioimmunoassay (RIA) for antiarrhythmic peptide (AAP) and study serum AAP levels in normal Sprague-Dawley rats (NR) and spontaneous hypertension rats (SHR), AAP-bovine serum alb... To establish a specific and sensitive radioimmunoassay (RIA) for antiarrhythmic peptide (AAP) and study serum AAP levels in normal Sprague-Dawley rats (NR) and spontaneous hypertension rats (SHR), AAP-bovine serum albumin (BSA) conjugate was prepared by ammonium bicarbonate method. The New Zealand rabbits were immunized by administering intradermally the conjugate. Then the rabbit anti-AAP serum was produced and iodinated AAP was made by Bolton-Hunter method. The RIA for serum AAP was set up and serum AAP levels in NR and SHR were determined. The minimal detectable range of the AAP RIA was (0.45±0.06) μg/L. The affinity constant for antiserum was 1.05×10 9 L/mol, and the rate of cross-reactivity with atrial peptide (AP) and human growth hormone (hGH) were 0.12 % and 0.20 %, respectively. The mean recovery rate of high, medium and low doses was 97.6 %, and the mean coefficients of variation for intra- and interbath-assay were (6.43±0.85) % and (9.62±1.04) %, respectively. The mean levels of AAP in NR with different age (3 months, 8-10 months and 18-20 months) were (1.75±0.13) μg/L, (1.74±0.11) μg/L and (1.79±0.15) μg/L, respectively, while those in SHR with different age (3 months, 8-10 months and 18-20 months) were (2.38±0.35) μg/L, (2.54±0.25) μg/L and (2.83±0.21) μg/L, respectively. The levels of serum AAP showed a positive correlation with blood pressure (r=0.8667, P<0.05). It was indicated that this AAP RIA had high specificity, high accuracy and good reproducibility. The levels of serum AAP had a close relation with blood pressure. 展开更多
关键词 antiarrhythmic peptide RADIOIMMUNOASSAY blood pressure spontaneous hypertension RATS
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RELATIONSHIP AMONG COX-2 PROTEIN EXPRESSION,PGs LEVELS AND BIOLOGIC BEHAVIOR IN OVARIAN CARCINOMA TISSUES
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作者 王敏 王欣彦 +1 位作者 唐丽霞 高岩 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2004年第4期284-288,共5页
Objective: To study the relationship among cyclooxygenase-2 (COX-2) protein expression, prostaglandins levels and biologic behavior in ovarian carcinoma tissues. Methods: The expression of COX-2 protein, levels of pro... Objective: To study the relationship among cyclooxygenase-2 (COX-2) protein expression, prostaglandins levels and biologic behavior in ovarian carcinoma tissues. Methods: The expression of COX-2 protein, levels of prostaglandin (PG)E2, 6-keto-PGF1α and thromboxane (TX)B2 in 54 biopsy specimens from patients with ovarian serous tumors which included three groups: 33 samples of ovarian serous carcinoma; 10 samples of borderline ovarian serous tumors and 11 samples of benign ovarian serous tumors and 10 samples of normal ovarian tissues were detected by Western blot analysis and radioimmunoassay to investigate their clinical significance. Results: The expression of COX-2 protein (82%, 27/33) and its relative content (20.08±3.53) in ovarian serous carcinoma tissues were statistically higher than those in benign ovarian serous tumor tissues and normal ovary tissues i.e., 0 and (15.04±0.12), 0 and (15.33±0.60) (P<0.05). The expression of COX-2 protein in borderline ovarian serous tumor tissues (90%, 9/10) and relative content (20.61±3.03) were statistically greater than those in benign ovarian serous tumor and normal ovary tissues (P<0.05). The expression of COX-2 protein and its relative content showed no significant differences in ovarian carcinoma tissues of different clinical stages (I to II and III to IV), different histological grades, with or without ascites and lymph metastasis. The levels of PGE2, 6-keto-PGF1 and TXB2 α in ovarian serous carcinoma tissues were statistically higher than in borderline ovarian serous tumor, benign ovarian serous tumor or normal ovarian tissues (P<0.05). No significant differences of the levels were found among borderline tissues, benign tissues and normal ovarian tissues (P>0.05). The levels of PGE2, 6-keto-PGF1 α and TXB2 showed no significant differences in ovarian carcinoma tissues with different clinical stages (I to II and III to IV), different histological grades, with or without ascites and lymph metastasis. COX-2 expression was correlated with the levels of PGE2, 6-KETO-PGF1 α and TXB2 (P<0.01). Conclusion: Our data suggest that COX-2 overexpression leads to increased PGE2, 6-KETA-PGF1 α and TXB2 biosynthesis, which may be mechanisms underlying the contribution of COX-2 to the development of ovarian serous carcinoma. BGF2, 6-keto-PGF1 and TXB2 may be helpful parameters of α diagnosis and differentiate diagnosis in ovarian serous carcinoma. 展开更多
关键词 Ovarian carcinoma CYCLOOXYGENASE-2 PROSTAGLANDINS Western blot RADIOIMMUNOASSAY
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THE MODIFIED RADIOIMMUNOASSAY OF SERUM INHIBIN AND ITS VALUE IN MONITORING OVARIAN TUMOR
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作者 隋龙 张令浩 +5 位作者 王成海 由振东 刘东 罗建华 金志军 朱明伟 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1999年第2期142-146,共5页
Objectives and Methods: A modified radioimmunoassay (RIA) of serum inhibin (INH) was developed and applied to measure serum INH contents in 39 fertile and 16 postmenopausal women. Thirty-three cases of ovarian tumors,... Objectives and Methods: A modified radioimmunoassay (RIA) of serum inhibin (INH) was developed and applied to measure serum INH contents in 39 fertile and 16 postmenopausal women. Thirty-three cases of ovarian tumors, including granulosa cell tumors and other kinds of ovarian tumors, were monitored by serum INH RIA. Results: The mean value of serum INH contents in follicular, peri-ovulatory and mid-luteal phases of fertile women were 9.48±7.10 pg/ml (2.04~18.53pg/ml), 19.04±9.73 pg/ml (3.49~33.26 pg/ml) and 131.13±110.81 pg/ml (3.49~ 341.10 pg/ml), respectively. Serum INH concentration was negatively correlated with serum FSH concentration, (rs=?0.483,P<0.01). Serum IHN contents were less than 3.6 pg/ml in normal postmenopausal women. The mean value of serum INH contents in ovarian granulosa cell tumor, thecoma, mucinous cystadenocarcinoma and malignant teratoma cases were significantly higher than that of other ovarian tumors, (P<0.01). Serum INH contents were elevated in ovarian granulosa cell tumor, thecoma, mucinous cystadenocaricinoma and endometrioid carcinoma cases with serum CA-125 values in normal range before operation, but serum INH contents decreased to normal range within one week after operation. And consecutive serum INH RIA could be a valuable tool in monitoring for therapeutic effect. Conclusion: Modified INH RIA was of convenient, time-saving and quantitative characteristics, especially with its high sensitivity (<1 pg/ml). There was a regular change of serum INH concentrations during menstrual cycle. INH could inhibit the synthesis and secretion of follicle stimulating hormone (FSH). INH would become a valuable marker for ovarian tumor. INH RIA combined with the measurement of serum CA-125 would be helpful to the early diagnosis, treatment and follow-up for ovarian cancer. 展开更多
关键词 INHIBIN Ovarian tumor Granulosa cell tumor RADIOIMMUNOASSAY CA-125
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Changes of Intestinal Caicitonin Gene-related Peptide in Acute Intestinal Radiation Sickness in Rats
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作者 吴康 蔡文琴 《Journal of Medical Colleges of PLA(China)》 CAS 1990年第2期123-127,197,共6页
The characteristic distribution of calcitonin gone-related peptide(CGRP)inthe small intestine of rats and its changes in acute intestinal radiation sickness(AIRS)were studied with immunocytochemistry(whole mount stret... The characteristic distribution of calcitonin gone-related peptide(CGRP)inthe small intestine of rats and its changes in acute intestinal radiation sickness(AIRS)were studied with immunocytochemistry(whole mount stretch preparations of the smallintestine and cryostat sections)and radio-immunoassay.It was found that in all the lay-ers of the intestinal walls,there were large amounts of CGRP immunoreactive(CGRP-I)nerve fibers which existed in especiaUy high density in the myenteric,submucosal andmucosal plexuses.There was also a rather high density of the nerves around the smallvessels of the small intestine and the intestinal crypts.Some CGRP-I neurons were seenin the myenteric and submucosal plexuses.In AIRS,the intestinal CGRP showed a dip-hasic change,in a lower level in the 24th h and a higher level in the 48th and 72nd h af-ter irradiation.The results indicate that CGRP may be related to the regulation of the motility,se-cretion,absorption,sensation,and regional blood flow of the gastrointestinal tract.Pro-bably,CGRP is released under the stress of AIRS and participates in the mechanism ofinjury through many ways especially through the influence on the regional blood flowand the increase of the permeability of blood vessels. 展开更多
关键词 CALCITONIN gene-related PEPTIDE small INTESTINE immunocytochermstry RADIOIMMUNOASSAY rat
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Preclinical and clinical studies on motilin
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作者 黄裕新 许才绂 +3 位作者 冯英明 廖洪 张少玲 王庆莉 《Journal of Medical Colleges of PLA(China)》 CAS 1993年第4期410-416,共7页
In the paper,we review our preclinical and clinical studies on motilin (MTL) in recentyears.Our studies have demonstrated for the first time that MTL was widely distributed in hu-man body fluids,fetal cerebrospinal fl... In the paper,we review our preclinical and clinical studies on motilin (MTL) in recentyears.Our studies have demonstrated for the first time that MTL was widely distributed in hu-man body fluids,fetal cerebrospinal fluid and digestive tract and have revealed that both humanand rabbit Mo cells(MTL-secreting cells)did secret intracavitarily.The endogenous MTL hadan accelerating effect on small bowel transmit time.Wheaten food taking could inhibit the releaseof MTL into plasma,and acupuncturing in certain acupoints could promote its secretion.The ris-es of plasma MTL levels were found in patients with liver cirrhosis,pregnant hypertension syn-drome or in stress state.MTL levels in plasma and gastroduodenal juices of gastric cancer pa-tients were elevated significantly;MTL levels in plasma were decreased in patients with non-ul-cer dyspepsia,bile-regurgitational gastritis or leukemia.We also probed into the phys-iopathological significance of changes in MTL levels in other human body fluids. 展开更多
关键词 MOTILIN FETUS body FLUIDS GASTROINTESTINAL motor activity acupuncture RADIOIMMUNOASSAY
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Effects of electric field stimulation on endogenous enkephalin content in artery wall
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作者 林树新 范瑾之 +1 位作者 刘琨 王复周 《Journal of Medical Colleges of PLA(China)》 CAS 1991年第4期304-308,共5页
After 20min electric field stimulation,L-enkephalin (L-ENK) content in the rabbitear artery declined from the control value of 38.99±17.29 to 12.55±1.96pg/mg of wet tissue(n=12),and M-enkephalin (M-ENK... After 20min electric field stimulation,L-enkephalin (L-ENK) content in the rabbitear artery declined from the control value of 38.99±17.29 to 12.55±1.96pg/mg of wet tissue(n=12),and M-enkephalin (M-ENK) content from 134.67±8.11 to 73.68±17.96pg/mg of wet tis-sue (n=6),both being significantly decreased.This effect was not influenced after blockade ofadrenergic receptor by yohimbine.Contractions of artery strips induced by electric field stimulationwere enhanced distinctly after pretreatment of the artery strips with naloxone,an opioid receptorblocker,and with pronase,but not after pretreatment with trypsin.The results suggested that the re-lease of endogenous enkephalins in the ear artery could be induced by electric field stimulation andx<sub>2</sub>-receptor might not participate in the release course,The release amount of ENK from the ar-terv strips increased when the stimulus freduency increased within the range of 2 Hz to 8 Hz. 展开更多
关键词 ENKEPHALINS RADIOIMMUNOASSAY ARTERY strip electric field STIMULATION
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Changes of Immunoreactive β-Endorphin in Plasma, Pituitary and Hypothalamus of Rats during Oxygen-Induced Convulsions
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作者 陶恒沂 倪国坛 《Journal of Medical Colleges of PLA(China)》 CAS 1989年第1期85-89,共5页
We observed for the first time the differences of immunoreactive β-endorphin(IR -β- EP) content in plasma, pituitary and hypothalamus of rats under various conditionsusing radioimmunoassay (RIA) and the effects of n... We observed for the first time the differences of immunoreactive β-endorphin(IR -β- EP) content in plasma, pituitary and hypothalamus of rats under various conditionsusing radioimmunoassay (RIA) and the effects of naloxone and β - endorphin (β- EP) antiserumon initial time of convulsions (ITC), severity of convulsions(SOC) and mortality on surface(MOS) of rats to hyperbaric oxygen(HBO). The results suggest thatβ- EP may partici-pate in the course of oxygen - induced convulsions and be one of endogenous convulsion - causingagents. 展开更多
关键词 Β-ENDORPHIN oxygen-induced CONVULSIONS naloxone radioimmunoassay ANTISERUM RATS
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Effects of ventromedial hypothalamic somatostatin on gastric somatostatin and acid secretion in rats
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作者 曲方 林葆城 +3 位作者 王成海 宋朝佑 白波 朱鹤年 《Journal of Medical Colleges of PLA(China)》 CAS 1991年第2期141-146,共6页
The effects of somatostatin and cysteamine injected into theventromedial nucleus of hypothalamus(VMH)on somatostatin-likeimmunoreactivity(SLI)concentration in the gastric antral mucosa and portal veinplasma,as well as... The effects of somatostatin and cysteamine injected into theventromedial nucleus of hypothalamus(VMH)on somatostatin-likeimmunoreactivity(SLI)concentration in the gastric antral mucosa and portal veinplasma,as well as the gastric acid secretion were studied in anesthetized rats.TheSLI concentration in the gastric antral mucosa and portal vein plasma and thegastric acid output were significantly increased one hour after the injection ofsomatostatin(0.5μg/0.5μ1)into the VMH.Four hours later,the SLI concentrationin the mucosa was still higher than the control level,while the SLI level in theplasma and the acid secretion were close to those of controls.On the contrary,the SLI concentration in the mucosa and plasma and the acid output weremarkedly decreased at four hours after the injection of cysteamine(15μg/0.5μ1)into the VMH.The present results suggest that both exogenous and endogenoussomatostatin in the VMH have a facilitatory action on the gastric somatostatinconcentration and its release as well as on the gastric acid secretion,thus provid-ing evidence for hypothalamic control of gastric somatostatin and acid secretion. 展开更多
关键词 HYPOTHALAMIC ventromedial NUCLEUS SOMATOSTATIN GASTRIC acid RADIOIMMUNOASSAY RATS
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Effects of acute brain injury on the contents of neurotensin in brain areas,pituitary gland and plasma in rats
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作者 刘志敏 林葆城 +2 位作者 王成海 路长林 赵小林 《Journal of Medical Colleges of PLA(China)》 CAS 1993年第1期46-51,共6页
The changes of immunoreactive neurotensin(ir-NT)contents in the brain areas,pituitary gland and plasma in the traumatized rats were observed in the present study.The results of radioimmunoassay exhibited significant c... The changes of immunoreactive neurotensin(ir-NT)contents in the brain areas,pituitary gland and plasma in the traumatized rats were observed in the present study.The results of radioimmunoassay exhibited significant changes of the ir-NT contents inthe hypothalamus,pituitary gland,plasma,injured tissue,hippocampus,central gray andspinal cord in the posttraumatic rats at different intervals.A predominant characteristicsof the changes of ir-NT levels in the brain areas,pituitary gland and plasma was thedramatical decrease at various times except for the hypothalamus,central gray andhippocampus with biphasic alterations.The ir-NT contents in the frontal cortex andpons-medulla also displayed changes to different extent under the acute craniocerebraltrauma condition.These results suggest that NT may play a role in the pathophysiologyof traumatic head injury. 展开更多
关键词 NEUROTENSIN acute BRAIN injury RADIOIMMUNOASSAY ANIMAL RATS
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Plasma leucine enkephalin content in patients with “Liver-blood deficiency” syndrome and clinical significance
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作者 SHI Lin Jie 1, CHEN Guo Lin 1, LI Xue Wen 1, SHU Yi Gang 2, ZHANG Xiang 1 and PANG Wei Hua 3 《World Journal of Gastroenterology》 SCIE CAS CSCD 1998年第1期97-97,共1页
Plasmaleucineenkephalincontentinpatientswith“Liverblooddeficiency”syndromeandclinicalsignificanceSHILinJi... Plasmaleucineenkephalincontentinpatientswith“Liverblooddeficiency”syndromeandclinicalsignificanceSHILinJie1,CHENGuoLin1,L... 展开更多
关键词 “Liver BLOOD deficiency” syndrome/pathophysiology enkephalin/blood RADIOIMMUNOASSAY
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The observation on the levels of VIP in the cerebral cortex of rats with bilateral hemispheric ischemia
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作者 王正品 蔡文琴 +1 位作者 彭英 刘兴材 《Journal of Medical Colleges of PLA(China)》 CAS 1992年第1期66-69,共4页
The rat vertebral and common carotid arteries were obstructed to cause bilateralhemispheric ischemia.Vasoactive intestinal peptide(VIP)in the rat cerebral cortex following 10~60min of ischemia was analysed by radioim... The rat vertebral and common carotid arteries were obstructed to cause bilateralhemispheric ischemia.Vasoactive intestinal peptide(VIP)in the rat cerebral cortex following 10~60min of ischemia was analysed by radioimmunoassay(RIA).The results showed that the levels ofVIP were significantly lower in the ischemic animals than controls(P【0.01).The distribution andmetabolism of VIP in the cerebral cortex and the probable mechanism during ischemia are dis-cussed. 展开更多
关键词 CEREBRAL CORTEX CEREBRAL ISCHEMIA vasoactive intestinal peptide RADIOIMMUNOASSAY RATS
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