BACKGROUND： Neuronal loss, synapse mutilation, and increasing malnourished axons are pathologically related to Alzheimer＇s disease. Microtubule-associated protein 2 （MAP2） is of importance for neuronal, axonal, an...BACKGROUND： Neuronal loss, synapse mutilation, and increasing malnourished axons are pathologically related to Alzheimer＇s disease. Microtubule-associated protein 2 （MAP2） is of importance for neuronal, axonal, and dendritic generation, extension, and stabilization, as well as for the regulation of synaptic plasticity. OBJECTIVE： To investigate the antagonistic effects of natural-cerebrolysin-containing serum on beta amyloid protein 1-40 （Aβ1-40）-induced neurotoxicity from the standpoints of cell proliferation, synaptogenesis, and cytoskeleton formation （MAP2 expression）. DESIGN, TIME AND SETTING： A paralleled, controlled, neural cell, and molecular biology experiment was performed at the Institute of Integrated Chinese and Western Medicine, Shenzhen Hospital, Southern Medical University between February 2006 and April 2008. MATERIALS： PC12 cells, derived from the rat central nervous system, were purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China. A β1-40 was provided by Sigma, USA. Natural-cerebrolysin was provided by Shenzhen Institute of Integrated Chinese and Western Medicine, China. The natural-cerebrolysin was predominantly composed of Renshen （Radix Ginseng）, Tianma （Rhizoma Gastrodiae）, and Yixingye （Ginkgo Leaf） in a proportion of 1：2：2. Following conventional water extraction technology, an extract （1：20） was prepared. Each gram of extract equaled 20 grams of crude drug. In a total of 12 adult male New Zealand rabbits, six underwent intragastric administration of natural-cerebrolysin extract for 1 month to prepare natural-cerebrolysin-containing serum, and the remaining six rabbits received intragastric administration of physiological saline to prepare normal blank serum. METHODS： An AIzheimer＇s disease in vitro model was induced in PC12 cells using Aβ1-40. The cells were incubated with varying doses of natural-cerebrolysin-containing serum （2.5%, 5%, and 10%）. Normal blank serum-treated PC12 cells served as a blank control group. MAIN OUTCOME MEASURES： Through the use of inverted phase contrast microscope, cell morphology and neurite growth were observed, neurite length was measured, and the percentage of neurite-positive cells was calculated. Cell proliferation rate was determined by MTT assay, and MAP 2 expression was detected by fluorescent immunocytochemistry. RESULTS： Following Aβ1-40 treatments, some PC12 cells were apoptotic/dying, and only a few short neurites were observed. Following interventions with natural-cerebrolysin-containing serum, the PC12 cells proliferated, there was an increased number of neurites, and neurite length was enhanced. After middle- and high-dose natural-cerebrolysin treatments, the percentage of neurite-positive cells, as well as the average length of neurites, was significantly greater than the normal blank serum-treated PC12 cells （P 〈 0.05 or P 〈 0.01）. Compared with the blank control group, MAP2 expression in the Aβ1-40-treated PC12 cells was significantly inhibited, and the cell proliferation rate was significantly decreased （P 〈 0.01）. Following incubations with natural-cerebrolysin-containing serum, MAP2 expression and cell proliferation rate in the PC12 cells were significantly increased in a dose-dependent manner, compared with treatments with blank control serum （P 〈 0.05 or P 〈 0.01 ）. CONCLUSION： Natural-cerebrolysin exhibited antagonistic effects on neurotoxicity in Aβ1-40 induced Alzheimer＇s disease in vitro models. These effects were likely related to cell proliferation and the upregulation of intracellular MAP2 expression.展开更多
Parkinson’s disease(PD)is characterized by pathological spontaneous beta oscillations(13 Hz-35 Hz)often observed in basal ganglia(BG)composed of subthalamic nucleus(STN)and globus pallidus(GPe)populations.From the vi...Parkinson’s disease(PD)is characterized by pathological spontaneous beta oscillations(13 Hz-35 Hz)often observed in basal ganglia(BG)composed of subthalamic nucleus(STN)and globus pallidus(GPe)populations.From the viewpoint of dynamics,the spontaneous oscillations are related to limit cycle oscillations in a nonlinear system;here we employ the bifurcation analysis method to elucidate the generating mechanism of the pathological spontaneous beta oscillations underlined by coupling strengths and intrinsic properties of the STN-GPe circuit model.The results reveal that the increase of inter-coupling strength between STN and GPe populations induces the beta oscillations to be generated spontaneously,and causes the oscillation frequency to decrease.However,the increase of intra-coupling(self-feedback)strength of GPe can prevent the model from generating the oscillations,and dramatically increase the oscillation frequency.We further provide a theoretical explanation for the role played by the inter-coupling strength of GPe population in the generation and regulation of the oscillations.Furthermore,our study reveals that the intra-coupling strength of the GPe population provides a switching mechanism on the generation of the abnormal beta oscillations:for small value of the intra-coupling strength,STN population plays a dominant role in inducing the beta oscillations;while for its large value,the GPe population mainly determines the generation of this oscillation.展开更多
Alzheimer’s disease（AD）is the most common type of dementia in elderly population.With a growing aging population not only in the United States but also in the worldwide,AD constitutes an emergent public health prob...Alzheimer’s disease（AD）is the most common type of dementia in elderly population.With a growing aging population not only in the United States but also in the worldwide,AD constitutes an emergent public health problem.展开更多
In this paper, we present the double Beta spline curved surface which is controlled by double parameters including the algorithm principles, the treatment of boundary conditions, the alternation of projection, the alg...In this paper, we present the double Beta spline curved surface which is controlled by double parameters including the algorithm principles, the treatment of boundary conditions, the alternation of projection, the algorithms of elimination hiddle line, the process to display and the primiples to produce the shaded curved surface. Based on all the above, a freedom surface modeling system (FSMS) is designed and some examples developed on FSMS are verified and analyzed.展开更多
This paper proposed beta trust model based on energy load balancing combines the recent achievements of the trust models in distributed networks, together with the characteristics of wireless sensor networks. The inte...This paper proposed beta trust model based on energy load balancing combines the recent achievements of the trust models in distributed networks, together with the characteristics of wireless sensor networks. The inter-node trust relation is established after an overall evaluation of node trust value based on the monitor results of the node packets forwarding behavior conducted by inter-node collaboration. Due to the node energy limitation in wireless sensor networks, energy load balancing mechanism is applied to prolong the node survival time. And the redundant routing protocol involves the presented trust model to develop the novel trust routing protocol of beta trust model based on energy load balancing. Simulation performance demonstrates that the beta trust model based on energy load balancing outperforms current schemes in energy consumption.展开更多
基金Supported by:Scientific and Technological Foundation of the National Administration of Traditional Chinese Medicine of China,No.02-03LP41the Scientific and Technological Key Project of Guangdong Province,No. 2006B35630007
文摘BACKGROUND： Neuronal loss, synapse mutilation, and increasing malnourished axons are pathologically related to Alzheimer＇s disease. Microtubule-associated protein 2 （MAP2） is of importance for neuronal, axonal, and dendritic generation, extension, and stabilization, as well as for the regulation of synaptic plasticity. OBJECTIVE： To investigate the antagonistic effects of natural-cerebrolysin-containing serum on beta amyloid protein 1-40 （Aβ1-40）-induced neurotoxicity from the standpoints of cell proliferation, synaptogenesis, and cytoskeleton formation （MAP2 expression）. DESIGN, TIME AND SETTING： A paralleled, controlled, neural cell, and molecular biology experiment was performed at the Institute of Integrated Chinese and Western Medicine, Shenzhen Hospital, Southern Medical University between February 2006 and April 2008. MATERIALS： PC12 cells, derived from the rat central nervous system, were purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China. A β1-40 was provided by Sigma, USA. Natural-cerebrolysin was provided by Shenzhen Institute of Integrated Chinese and Western Medicine, China. The natural-cerebrolysin was predominantly composed of Renshen （Radix Ginseng）, Tianma （Rhizoma Gastrodiae）, and Yixingye （Ginkgo Leaf） in a proportion of 1：2：2. Following conventional water extraction technology, an extract （1：20） was prepared. Each gram of extract equaled 20 grams of crude drug. In a total of 12 adult male New Zealand rabbits, six underwent intragastric administration of natural-cerebrolysin extract for 1 month to prepare natural-cerebrolysin-containing serum, and the remaining six rabbits received intragastric administration of physiological saline to prepare normal blank serum. METHODS： An AIzheimer＇s disease in vitro model was induced in PC12 cells using Aβ1-40. The cells were incubated with varying doses of natural-cerebrolysin-containing serum （2.5%, 5%, and 10%）. Normal blank serum-treated PC12 cells served as a blank control group. MAIN OUTCOME MEASURES： Through the use of inverted phase contrast microscope, cell morphology and neurite growth were observed, neurite length was measured, and the percentage of neurite-positive cells was calculated. Cell proliferation rate was determined by MTT assay, and MAP 2 expression was detected by fluorescent immunocytochemistry. RESULTS： Following Aβ1-40 treatments, some PC12 cells were apoptotic/dying, and only a few short neurites were observed. Following interventions with natural-cerebrolysin-containing serum, the PC12 cells proliferated, there was an increased number of neurites, and neurite length was enhanced. After middle- and high-dose natural-cerebrolysin treatments, the percentage of neurite-positive cells, as well as the average length of neurites, was significantly greater than the normal blank serum-treated PC12 cells （P 〈 0.05 or P 〈 0.01）. Compared with the blank control group, MAP2 expression in the Aβ1-40-treated PC12 cells was significantly inhibited, and the cell proliferation rate was significantly decreased （P 〈 0.01）. Following incubations with natural-cerebrolysin-containing serum, MAP2 expression and cell proliferation rate in the PC12 cells were significantly increased in a dose-dependent manner, compared with treatments with blank control serum （P 〈 0.05 or P 〈 0.01 ）. CONCLUSION： Natural-cerebrolysin exhibited antagonistic effects on neurotoxicity in Aβ1-40 induced Alzheimer＇s disease in vitro models. These effects were likely related to cell proliferation and the upregulation of intracellular MAP2 expression.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61473208 and 61876132)the Tianjin Research Program of Application Foundation and Advanced Technology,China(Grant No.15JCYBJC47700).
文摘Parkinson’s disease(PD)is characterized by pathological spontaneous beta oscillations(13 Hz-35 Hz)often observed in basal ganglia(BG)composed of subthalamic nucleus(STN)and globus pallidus(GPe)populations.From the viewpoint of dynamics,the spontaneous oscillations are related to limit cycle oscillations in a nonlinear system;here we employ the bifurcation analysis method to elucidate the generating mechanism of the pathological spontaneous beta oscillations underlined by coupling strengths and intrinsic properties of the STN-GPe circuit model.The results reveal that the increase of inter-coupling strength between STN and GPe populations induces the beta oscillations to be generated spontaneously,and causes the oscillation frequency to decrease.However,the increase of intra-coupling(self-feedback)strength of GPe can prevent the model from generating the oscillations,and dramatically increase the oscillation frequency.We further provide a theoretical explanation for the role played by the inter-coupling strength of GPe population in the generation and regulation of the oscillations.Furthermore,our study reveals that the intra-coupling strength of the GPe population provides a switching mechanism on the generation of the abnormal beta oscillations:for small value of the intra-coupling strength,STN population plays a dominant role in inducing the beta oscillations;while for its large value,the GPe population mainly determines the generation of this oscillation.
基金supported by the Brazilian funding agencies:CNPq(Conselho Nacional de Desenvolvimento Científico e Tecnológico)CAPES(Coordenacao de Aperfeicoamento de Pessoal de Nível Superior)-No:5614112Student Agreement Program for post graduation–PEC-PG.
文摘Alzheimer’s disease（AD）is the most common type of dementia in elderly population.With a growing aging population not only in the United States but also in the worldwide,AD constitutes an emergent public health problem.
文摘In this paper, we present the double Beta spline curved surface which is controlled by double parameters including the algorithm principles, the treatment of boundary conditions, the alternation of projection, the algorithms of elimination hiddle line, the process to display and the primiples to produce the shaded curved surface. Based on all the above, a freedom surface modeling system (FSMS) is designed and some examples developed on FSMS are verified and analyzed.
文摘This paper proposed beta trust model based on energy load balancing combines the recent achievements of the trust models in distributed networks, together with the characteristics of wireless sensor networks. The inter-node trust relation is established after an overall evaluation of node trust value based on the monitor results of the node packets forwarding behavior conducted by inter-node collaboration. Due to the node energy limitation in wireless sensor networks, energy load balancing mechanism is applied to prolong the node survival time. And the redundant routing protocol involves the presented trust model to develop the novel trust routing protocol of beta trust model based on energy load balancing. Simulation performance demonstrates that the beta trust model based on energy load balancing outperforms current schemes in energy consumption.
