Five stems of rapeseed with abundant black microsclerotia were collected from Huangyuan County of Qinghai Province,China,and fungal isolates were obtained from the stems.They were identified based on morphology,molecu...Five stems of rapeseed with abundant black microsclerotia were collected from Huangyuan County of Qinghai Province,China,and fungal isolates were obtained from the stems.They were identified based on morphology,molecular features and specific PCR detection.The results showed that the 10 fungal isolates belonged to Verticillium longisporum lineage A1/D3.One of the 10 isolates(HW7-1)was tested for virulence on three species of rapeseed,including B.napus Zhongshuang 9,B.rapa Qingyou 9 and B.juncea Tayou 2 by conidia inoculation of HW7-1 on roots of young seedlings.Control seedlings were inoculated with V.dahliae conidia or water alone.The seedlings of these treatments were transplanted in culture mix and incubated in a growth chamber(20℃).Results suggested that the control seedlings of three cultivars appeared quite healthy,while the seedlings inoculated with HW7-1 turned yellowing leaves,seedling stunting or even death after 22 days post-inoculation.V.longisporum was re-isolated from he yellow leaves,thus fulfilling Koch's postulates.Moreover,compared to the control treatments,inoculation with HW7-1 caused flowering delay and seed yield reduction on Tayou 2 with production of microsclerotia on the stems.To our knowledge,this is the first report of V.longisporum lineage A1/D3 on rapeseed in northwestern China.展开更多
Large-scale crop mapping using remote sensing data is of great significance for agricultural production, food security and the sustainable development of human societies. Winter rapeseed is an important oil crop in Ch...Large-scale crop mapping using remote sensing data is of great significance for agricultural production, food security and the sustainable development of human societies. Winter rapeseed is an important oil crop in China that is mainly distributed in the Yangtze River Valley. Traditional winter rapeseed mapping practices are insufficient since they only use the spectral characteristics during the critical phenological period of winter rapeseed, which are usually limited to a small region and cannot meet the needs of large-scale applications. In this study, a novel phenology-based winter rapeseed index(PWRI) was proposed to map winter rapeseed in the Yangtze River Valley. PWRI expands the date window for distinguishing winter rapeseed and winter wheat, and it has good separability throughout the flowering period of winter rapeseed. PWRI also improves the separability of winter rapeseed and winter wheat, which traditionally have been two easily confused winter crops. A PWRI-based method was applied to the Middle Reaches of the Yangtze River Valley to map winter rapeseed on the Google Earth Engine platform. Time series composited Sentinel-2 data were used to map winter rapeseed with 10 m resolution. The mapping achieved a good result with overall accuracy and kappa coefficients exceeding 92% and 0.85, respectively. The PWRI-based method provides a new solution for high spatial resolution winter rapeseed mapping at a large scale.展开更多
Rapeseed(Brassica napus)is an oil crop grown worldwide,making it a key plant species in molecular breeding research.However,the complexity of its polyploid genome increases sequencing costs and reduces sequencing accu...Rapeseed(Brassica napus)is an oil crop grown worldwide,making it a key plant species in molecular breeding research.However,the complexity of its polyploid genome increases sequencing costs and reduces sequencing accuracy.Target capture coupled with high-throughput sequencing is an efficient approach for detecting genetic variation at genomic regions or loci of interest.In this study,588 resequenced accessions of rapeseed were used to develop a target capture sequencing SNP genotyping platform named BnaPan50T.The platform comprised 54,765,with 54,058 resequenced markers from the pan-genome,and 855 variant trait-associated markers for 12 agronomic traits.The capture quality of BnaPan50T was demonstrated well in 12 typical accessions.Compared with a conventional genotyping array,BnaPan50T has a high SNP density and a high proportion of SNPs in unique physical positions and in annotated functional genes,promising wide application.Target capture sequencing and wholegenome resequencing in 90 doubled-haploid lines yielded 60%specificity,78%uniformity within tenfold coverage range,and 93%genotyping accuracy for the platform.BnaPan50T was used to construct a genetic map for quantitative trait loci(QTL)mapping,identify 21 unique QTL,and predict several candidate genes for yield-related traits in multiple environments.A set of 132 core SNP loci was selected from BnaPan50T to construct DNA fingerprints and germplasm identification resources.This study provides genomics resources to support target capture sequencing,genetic analysis and genomic breeding of rapeseed.展开更多
Rapeseed(Brassica napus L.)harvesting method is critical since it significantly determines the seed yield,oil quality,and industrial efficiency.This study investigated the influences of harvesting methods on the quali...Rapeseed(Brassica napus L.)harvesting method is critical since it significantly determines the seed yield,oil quality,and industrial efficiency.This study investigated the influences of harvesting methods on the quality of cold-pressed rapeseed oil of two varieties.Oil color,peroxide value(POV),tocopherol content,fatty acid composition,and polarity of total polyphenols(PTP)contents of two rapeseed varieties in Huanggang and Xiangyang were compared through artificially simulated combined harvesting and two-stage harvesting.Results showed significant differences in the quality of rapeseed oil between the two harvesting methods.The red value(R-value),POV,total tocopherol contents,linoleic and linolenic acid content,and PTP content of the pressed rapeseed oil prepared by the combined harvesting method were about 27.6,5.7,15.8,2.0,0.5,and 28.6%lower than those of the oil produced from the two-stage harvesting method,respectively.Xiangyang and Huayouza62 performed better in the two regions and two varieties,respectively.To sum up,the rapeseed oil obtained 41–44 days after final flowering of combined harvesting,35 days after final flowering,and six days of post-ripening of the two-stage harvesting had the best quality.展开更多
Hubei Province is the main winter rapeseed producing area in China,and has the national advantage and characteristic industrial cluster of rape.The protection zone for the production of important agricultural products...Hubei Province is the main winter rapeseed producing area in China,and has the national advantage and characteristic industrial cluster of rape.The protection zone for the production of important agricultural products of rapeseed in Hubei Province also ranks first as that in Hunan Province.This paper studied the winter rape industry,the important agricultural product production protection zone of rapeseed,the provincial double-low high-quality rape protection zone and the industrial cluster of rape in Jianghan Plain in Hubei Province,and analyzed the main problems existing in the development of rapeseed production protection zone and industrial cluster of rape.Finally,it came up with strategies including promoting national rape varieties,overcoming the problem of rape"agricultural chip",promoting the"rice+rape"rotation model,and strengthening the protection of intellectual property rights of brand marks.展开更多
The statistics of the number of rapeseed seedlings are very important for breeders and planters to conduct seed quality testing,field crop management and yield estimation.Calculating the number of seedlings is ineffic...The statistics of the number of rapeseed seedlings are very important for breeders and planters to conduct seed quality testing,field crop management and yield estimation.Calculating the number of seedlings is inefficient and cumbersome in the traditional method.In this study,a method was proposed for efficient detection and calculation of rapeseed seedling number based on improved you only look once version 5(YOLOv5)to identify objects and deep-sort to perform object tracking for rapeseed seedling video.Coordinated attention(CA)mechanism was added to the trunk of the improved YOLOv5s,which made the model more effective in identifying shaded,dense and small rapeseed seedlings.Also,the use of the GSConv module replaced the standard convolution at the neck,reduced model parameters and enabled it better able to be equipped for mobile devices.The accuracy and recall rate of using improved YOLOv5s on the test set by 1.9%and 3.7%compared to 96.2%and 93.7%of YOLOv5s,respectively.The experimental results showed that the average error of monitoring the number of seedlings by unmanned aerial vehicles(UAV)video of rapeseed seedlings based on improved YOLOv5s combined with depth-sort method was 4.3%.The presented approach can realize rapid statistics of the number of rapeseed seedlings in the field based on UAV remote sensing,provide a reference for variety selection and precise management of rapeseed.展开更多
Rapeseed(Brassica napus L.) is the largest oilseed crop in China and accounts for about 20% of world production.For the last 10 years,the production,planting area,and yield of rapeseed have been stable,with improvemen...Rapeseed(Brassica napus L.) is the largest oilseed crop in China and accounts for about 20% of world production.For the last 10 years,the production,planting area,and yield of rapeseed have been stable,with improvement of seed quality and especially seed oil content.China is among the leading countries in rapeseed genomic research internationally,having jointly with other countries accomplished the whole genome sequencing of rapeseed and its two parental species,Brassica oleracea and Brassica rapa.Progress on functional genomics including the identification of QTL governing important agronomic traits such as yield,seed oil content,fertility regulation,disease and insect resistance,abiotic stress,nutrition use efficiency,and pod shattering resistance has been achieved.As a consequence,molecular markers have been developed and used in breeding programs.During 2005–2014,215 rapeseed varieties were registered nationally,including 210 winter-and 5 spring-type varieties.Mechanization across the whole process of rapeseed production was investigated and operating instructions for all relevant techniques were published.Modern techniques for rapeseed field management such as high-density planting,controlled-release fertilizer,and biocontrol of disease and pests combined with precision tools such as drones have been developed and are being adopted in China.With the application of advanced breeding and production technologies,in the near future,the oil yield and quality of rapeseed varieties will be greatly increased,and more varieties with desirable traits,especially early maturation,high yield,high resistance to biotic and abiotic stress,and suitability for mechanized harvesting will be developed.Application of modern technologies on the mechanized management of rapeseed will greatly increase grower profit.展开更多
Background: Rapeseed cake is a good source of protein for animal feed but its utilization is limited due to the presence of anti-nutritional substances, such as glucosinolates (GIs), phytic acid, tannins etc. In th...Background: Rapeseed cake is a good source of protein for animal feed but its utilization is limited due to the presence of anti-nutritional substances, such as glucosinolates (GIs), phytic acid, tannins etc. In the present study, a solid state fermentation (SSF) using Aspergillus niger was carried out with the purpose of degrading glucosinolates and improving the nutritional quality of rapeseed cake (RSC). The effects of medium composition and incubation conditions on the GIs content in fermented rapeseed cake (FRSC) were investigated, and chemical composition and amino acid in vitro digestibility of RSC substrate fermented under optimal conditions were determined. Results: After 72 h of incubation at 34℃, a 76.89% decrease in GIs of RSC was obtained in solid medium containing 70% RSC, 30% wheat bran at optimal moisture content 60% (w/w). Compared to unfermented RSC, trichloroacetic acid soluble protein (TCA-SP), crude protein and ether extract contents of the FRSC were increased (P〈 0.05) 103.71, 23.02 and 23.54%, respectively. As expected, the contents of NDF and phytic acid declined (P〈 0.05) by 9.12 and 44.60%, respectively. Total amino acids (TAA) and essential amino acids (EAA) contents as well as AA in vitro digestibility of FRSC were improved significantly (P 〈 0.05). Moreover, the enzyme activity of endoglucanase, xylanase, acid protease and phytase were increased (P 〈 0.05) during SSF. Conclusions: Our results indicate that the solid state fermentation offers an effective approach to improving the quality of proteins sources such as rapeseed cake.展开更多
Background: Two experiments were conducted to estimate the net energy(NE) of corn, soybean meal, expel er-pressed rapeseed meal(EP-RSM) and solvent-extracted rapeseed meal(SE-RSM) using indirect calorimetry and to val...Background: Two experiments were conducted to estimate the net energy(NE) of corn, soybean meal, expel er-pressed rapeseed meal(EP-RSM) and solvent-extracted rapeseed meal(SE-RSM) using indirect calorimetry and to validate the NE of these four ingredients using pig growth performance.Methods: In Exp.1, 24 barrows(initial BW = 36.4 ± 1.6 kg) were allotted to 1 of 4 diets which included a corn basal diet,a corn-soybean meal basal diet and two rapeseed meal diets containing 20% EP-RSM(9.5% ether extract) or SE-RSM(1.1% ether extract) substituted for corn and soybean meal. The design allowed the calculation of NE values of corn,soybean meal and rapeseed meals according to the difference method. In Exp.2, 175 growing pigs(initial BW = 36.0± 5.2 kg) were fed 1 of 5 diets for 28 d, with five pigs per pen and seven replications(pens) per treatment in order to validate the measured energy values. Diets were a corn-soybean meal diet and four diets including 10% or 20% EP-RSM and 10% or 20% SE-RSM.Results: The NE of corn, soybean meal, EP-RSM and SE-RSM were 12.46, 11.34, 11.71 and 8.83 MJ/kg DM, respectively. The NE to ME ratio of corn(78%) was similar to tabular values, however, the NE to ME ratios of soybean meal(70%) and rapeseed meal(76%) were greater than tabular values. The greater NE value in EP-RSM than in SE-RSM is consistent with its higher EE content. Increasing EP-RSM or SE-RSM did not affect the growth performance of pigs and the caloric efficiency of NE was comparable for al diets.Conclusions: The NE of EP-RSM was similar to soybean meal, and both were greater than SE-RSM. The DE, ME and NE values measured in Exp.1 are confirmed by results of Exp. 2 with comparable caloric efficiencies of DE, ME or NE for all diets.展开更多
Rapeseed is a very important oil crop in China; however, its production is challenging due to the absence of effective weed management strategies. This is predominantly because of a shortage of herbicide resistance ge...Rapeseed is a very important oil crop in China; however, its production is challenging due to the absence of effective weed management strategies. This is predominantly because of a shortage of herbicide resistance genes. Acetohydroxyacid synthase (AHAS) herbicides inhibit AHAS, a key enzyme involved in branched-chain amino acid synthesis that is required for plant growth. A rapeseed line designated M342 with AHAS herbicide resistance was developed through seed muta- genesis and was studied to assess the level and mode of inheritance of the resistance and to identify the molecular basis of resistance. M342 possessed a high level of cross-resistance to sulfonylureas (SUs) and imidazolinones (IMIs). This resistance was due to AHAS insensitivity to these herbicides and was inherited as a dominant trait conferred by a single nuclear-encoded gene. Molecular analysis revealed the presence of a Trp574Leu mutation in M342, and an allele-specific cleaved amplified polymorphic sequence (AS-CAPS) marker was developed and cosegregated with herbicide resistance in the F2, BC1, and BC2 populations. This mutation altered the transcript levels of BnAHAS1 and BnAHAS3 in M342 compared with those in the wild type, but it did not affect the agronomic or quality traits. The simple genetic inheritance of this mutation and the availability of the cleaved amplified polymorphic sequence (CAPS) marker and herbicide resistance gene should facilitate the development of herbicide-resistant rapeseed cultivars for effective weed control in China.展开更多
Strigolactones(SLs)are newly discovered plant hormones which regulate the normal development of different plant organs,especially root architecture.Lateral root formation of rapeseed seedlings before winter has great ...Strigolactones(SLs)are newly discovered plant hormones which regulate the normal development of different plant organs,especially root architecture.Lateral root formation of rapeseed seedlings before winter has great effects on the plant growth and seed yield.Here,we treated the seedlings of Zhongshuang 11(ZS11),an elite conventional rapeseed cultivar,with different concentrations of GR24(a synthetic analogue of strigolactones),and found that a low concentration(0.18μmol L–1)of GR24 could significantly increase the lateral root growth,shoot growth,and root/shoot ratio of seedlings.RNA-Seq analysis of lateral roots at 12 h,1 d,4 d,and 7 d after GR24 treatment showed that 2301,4626,1595,and 783 genes were significantly differentially expressed,respectively.Function enrichment analysis revealed that the plant hormone transduction pathway,tryptophan metabolism,and the phenylpropanoid biosynthesis pathway were over-represented.Moreover,transcription factors,including AP2/ERF,AUX/IAA,NAC,MYB,and WRKY,were up-regulated at 1 d after GR24 treatment.Metabolomics profiling further demonstrated that the amounts of various metabolites,such as indole-3-acetic acid(IAA)and cis-zeatin were drastically altered.In particular,the concentrations of endogenous IAA significantly decreased by 52.4 and 75.8%at 12 h and 1 d after GR24 treatment,respectively.Our study indicated that low concentrations of exogenous SLs could promote the lateral root growth of rapeseed through interaction with other phytohormones,which provides useful clues for the effects of SLs on root architecture and crop productivity.展开更多
Biological yield indicates the potential for increasing yield.Leaf carbon metabolism plays an important role in the biomass accumulation of rapeseed(Brassica napus L.).Field experiments with the hybrid HZ62(with a con...Biological yield indicates the potential for increasing yield.Leaf carbon metabolism plays an important role in the biomass accumulation of rapeseed(Brassica napus L.).Field experiments with the hybrid HZ62(with a conventional plant architecture)grown in 2016–2017,and HZ62 and accession 1301(with a compact plant architecture)grown in 2017–2018 were conducted to characterize the physiological and proteomic responses of leaf photosynthetic carbon metabolism to density and row spacing configurations.The densities were set at 15×10;ha^(-1)(D1),30×10^(4)ha^(-1)(D2),and 45×10^(4)ha^(-1)(D3)(main plot),with row spacings of 15 cm(R15),25 cm(R25),and 35 cm(R35)(subplot).Individual and plant population biomass accumulation was greatest at R25,R15,and R15 for D1,D2,and D3,respectively,for both genotypes.In comparison with D1 R25,the individual aboveground biomass of HZ62 decreased by60.2%,whereas the population biomass increased by 31.9%,and the individual biomass of genotype1301 decreased by 54.0%and the population biomass increased by 53.9%at D3 R15.Leaf carbon metabolic enzymes varied between genotypes at flowering stage.In contrast to D1 R25,at D3 R15 the activities of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)and sucrose phosphate synthase(SPS)and the contents of starch,sucrose and soluble sugars in leaves were significantly decreased in HZ62 and increased in genotype 1301.The activities of fructose-1,6-bisphosphatase(FBPase)decreased,in consistency with the abundance of fructose-bisphosphate aldolase in HZ62.In contrast,sucrose synthase(Su Sy)activity appeared to decrease in both genotypes,but a significant increase in abundance of a protein with sucrose synthase was found in the 1301 genotype by proteomic analysis.With increased density and reduced row spacing,the expression of most key proteins involved in carbon metabolism was elevated,and enzyme activity and carbon assimilate content were increased in 1301,whereas HZ62 showed the opposite trend,indicating that the compact plant type can accumulate more population biomass with denser planting.展开更多
Pod shatter resistance of rapeseed is of great importance for modem farming practice. In order to determine the combining ability of elite inbred lines and the breeding potential of rapeseed hybrids in terms of pod sh...Pod shatter resistance of rapeseed is of great importance for modem farming practice. In order to determine the combining ability of elite inbred lines and the breeding potential of rapeseed hybrids in terms of pod shatter resistance, analysis of a 6×6 incomplete diallel cross was conducted at two locations. Results showed that a significant variation existed among breeding lines and their F1 hybrids for pod shatter resistant index (SRI), pod length and width. Pod shatter resistance was significantly positively correlated with pod length. The general combining ability (GCA) effects (GCA=l.58) played a more important role than specific combining ability (SCA) effects (SCA=0.20) for pod shatter trait. The elite lines R1, 1019B and 1055B displayed significant positive GCA effects for pod shatter resistance. Four crosses (1019B×R1, 1015B×R1, 6098B×R1, and 8908B×R1) with high mean performance and positive SCA effects were recommended for developing new hybrids for mechanical harvest in the middle reaches of the Yangtze River.展开更多
Background: The nutritional value of rapeseed meal may be variable due to the variation of its chemical composition. And a precise understandin9 of the nutritional value of an ingredient is beneficial for the accurat...Background: The nutritional value of rapeseed meal may be variable due to the variation of its chemical composition. And a precise understandin9 of the nutritional value of an ingredient is beneficial for the accurate diet formulation and reduction of feed costs. This study was conducted to determine the chemical composition, dicjestible energy (DE) and metabolizable enercjy (ME) content, and apparent ileal digestibility (AID) and standardized ileal dicjestibility (SID) of amino acids (AA) for growincj pigs. Thirteen solvent-extracted double-low rapeseed meal (DLIRSM) samples were obtained from the main double-low rapeseed producing areas in China. Methods: The DE and ME contents of the 13 DLRSM samples were measured in cjrowin9 pigs (six pigs per DLRSM sample, average initial body weicjht (BW) =48.3 kg). The AID and SID of AA of 10 DLRSM samples were determined in 12 crossbred barrows (average initial BW = 35.3 kcj) by using two 6 x 6 Latin square designs. Each Latin square comprised one N-flee diet and 5 DLRSM test diets. Results: The chemical composition of DLRSM varied among samples, and the coefficient of variation was greater than ]0 % for ether extract (EE), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium (Ca), and total 91ucosinolates. The AA content of DLRSM varied among samples especially for lysine (Lys) and methionine (Met). On a dry matter (DM) basis, the apparent total tract digestibility (ATFD) of gross energy (GE), the DE and ME and the ME:DE ratio of DLRSM averaged 62.39 %, 2862 kcal/k9 and 2723 kcal/kcj, and 94.95 %, respectively. The mean value of S1D of Lys was 70.52 % which varied from 66.54-76.54 %. The SID of crude protein (CP), Met, and threonine (Thr) averaged 72.81%, 82.41%, and 69.76 %, respectively. Conclusions: There was great variability in chemical composition especially in the concentration of EE, NDF and ADF, but no significant differences in energy content of the DLIRSM samples were observed. In addition, the AID and SID of all AA were relatively similar amoncj DLRSM samples except for that of Lys.展开更多
A yield gap analysis for rapeseed(Brassica napus L.) is critical to meeting the oil demand by identifying yield potential and yield constraints. In this study, potential yield(Y_p), attainable yield(Y_(att)), and actu...A yield gap analysis for rapeseed(Brassica napus L.) is critical to meeting the oil demand by identifying yield potential and yield constraints. In this study, potential yield(Y_p), attainable yield(Y_(att)), and actual yield(Y_(act)) for winter rapeseed were determined in five different zones of China. A boundary line approach was adopted to calculate Y_p, based on a large-scale field experimental database. A meta-analysis was conducted on the data obtained from 118 published studies to evaluate the effects of agronomic factors on rapeseed yield. The main results indicated that farmers only achieved 37–56% of the yield potential across the zones. The low altitude areas(L-URY) and lower reaches(LRY) of the Yangtze River Basin(YRB), China had high yield levels. The total yield gap was 1 893 kg ha^(–1), due to the agronomic management factors, environmental factors, and socioeconomic factors. The meta-analysis showed that weed control and drainage were the best management practices to improve yields(45.6 and 35.3%, respectively), and other practices improved yields by 17.1–21.6%. Consequently, to narrow the yield gap over the short term, the study could focus on techniques that are easily implemented to farmers.展开更多
The timely and rapid mapping of rapeseed planting areas is desirable for national food security. Most current rapeseed mapping methods depend strongly on images with good observations obtained during the flowering sta...The timely and rapid mapping of rapeseed planting areas is desirable for national food security. Most current rapeseed mapping methods depend strongly on images with good observations obtained during the flowering stages. Although vegetation indices have been proposed to identify the rapeseed flowering stage in some areas, automatically mapping rapeseed planting areas in large regions is still challenging.We developed an automatic phenology-and pixel-based algorithm(APPA) by integrating Landsat 8 and Sentinel-1 satellite data. We found that the Normalized Rapeseed Flowering Index shows unique spectral characteristics during the flowering and post-flowering periods, which distinguish rapeseed parcels from other land-use types(urban, water, forest, grass, maize, wheat, barley, and soybean). To verify the robustness of APPA, we applied APPA to seven areas in five rapeseed-producing countries with flowering images unavailable. The rapeseed maps by APPA showed consistently high accuracies with producer accuracies of 0.87–0.93 and F-scores of 0.92–0.95 based on 4503 verification samples. They showed high spatial consistency at the pixel level with the land cover Scientific Expertise Centres(SEC) map in France,Crop Map of England in United Kingdom, national-scale crop-and land-cover map of Germany, and Annual Crop Inventory in Canada at the pixel level. We propose APPA as a highly promising method for automatically and efficiently mapping rapeseed areas.展开更多
With the increasing promotion of simplified rapeseed cultivation in recent years,the development of cultivars with high resistance to herbicides is urgently needed.We previously developed M342,which shows sulfonylurea...With the increasing promotion of simplified rapeseed cultivation in recent years,the development of cultivars with high resistance to herbicides is urgently needed.We previously developed M342,which shows sulfonylurea herbicide resistance,by targeting acetohydroxyacid synthase(AHAS),a key enzyme in branched-chain amino acid synthesis.In the present study,we used a progeny line derived from M342 for an additional round of ethyl methane sulfonate mutagenesis,yielding the novel mutant DS3,which harbored two mutations in AHAS genes and showed high sulfonylurea resistance.One mutation was the substitution Trp574 Leu,as in M342,according to Arabidopsis protein sequencing.The other site was a newly recognized substitution,Pro197 Leu.A KASP marker targeting Pro197 Leu was developed and reliably predicted the response to sulfonylurea herbicides in the F2 population.The combination of Trp574 Leu and Pro197 Leu in DS3 produced a synergistic effect that greatly increased herbicide resistance.Analysis of the protein structures of AHAS1 and AHAS3 in wild-type and single-gene mutant plants revealed three-dimensional protein conformational changes that could account for differences in herbicide resistance characteristics including toxicity tolerance,AHAS enzyme activity,and AHAS gene expression.展开更多
financially supported by the Key R&D Program of Jiangxi Province of China (20152ACF60010);the Key Projects of Hunan Science and Technology Support Program of China (Application research of Guiye A male sterile lin...financially supported by the Key R&D Program of Jiangxi Province of China (20152ACF60010);the Key Projects of Hunan Science and Technology Support Program of China (Application research of Guiye A male sterile line);the Research Project of Humanities and Social Sciences in Universities of Jiangxi Province, China(JC1315);supported by Emmy Noether DFG grant MA 6473/1-1展开更多
Melatonin is a naturally occurring compound in plants. Here, we tested the effect of exogenous melatonin on rapeseed(Brassica napus L.) grown under salt stress. Application of 30 μmol L^-1 melatonin alleviated salt...Melatonin is a naturally occurring compound in plants. Here, we tested the effect of exogenous melatonin on rapeseed(Brassica napus L.) grown under salt stress. Application of 30 μmol L^-1 melatonin alleviated salt-induced growth inhibition, and the shoot fresh weight, the shoot dry weight, the root fresh weight, and the root dry weight of seedlings treated with exogenous melatonin increased by 128.2, 142.9, 122.2, and 124.2%, respectively, compared to those under salt stress. In addition, several physiological parameters were evaluated. The activities of antioxidant enzymes including peroxidase(POD), catalase(CAT) and ascorbate peroxidase(APX) were enhanced by 16.5, 19.3, and 14.2% compared to their activities in plants without exogenous melatonin application under salt stress, while the H2O2 content was decreased by 11.2% by exogenous melatonin. Furthermore, melatonin treatment promoted solute accumulation by increasing the contents of proline(26.8%), soluble sugars(15.1%) and proteins(58.8%). The results also suggested that higher concentrations(〉50 μmol L^-1) of melatonin could attenuate or even prevent the beneficial effects on seedling development. In conclusion, application of a low concentration of exogenous melatonin to rapeseed plants under salt stress can improve the H2O2-scavenging capacity by enhancing the activities of antioxidant enzymes such as POD, CAT and APX, and can also alleviate osmotic stress by promoting the accumulation of osmoregulatory substances such as soluble proteins, proline, and water soluble glucan. Ultimately, exogenous melatonin facilitates root development and improves the biomass of rapeseed seedlings grown under salt stress, thereby effectively alleviating the damage of salt stress in rapeseed seedlings.展开更多
基金supported by the Earmarked Fund for CARS-12 from National Modern Agricultural Technology System.
文摘Five stems of rapeseed with abundant black microsclerotia were collected from Huangyuan County of Qinghai Province,China,and fungal isolates were obtained from the stems.They were identified based on morphology,molecular features and specific PCR detection.The results showed that the 10 fungal isolates belonged to Verticillium longisporum lineage A1/D3.One of the 10 isolates(HW7-1)was tested for virulence on three species of rapeseed,including B.napus Zhongshuang 9,B.rapa Qingyou 9 and B.juncea Tayou 2 by conidia inoculation of HW7-1 on roots of young seedlings.Control seedlings were inoculated with V.dahliae conidia or water alone.The seedlings of these treatments were transplanted in culture mix and incubated in a growth chamber(20℃).Results suggested that the control seedlings of three cultivars appeared quite healthy,while the seedlings inoculated with HW7-1 turned yellowing leaves,seedling stunting or even death after 22 days post-inoculation.V.longisporum was re-isolated from he yellow leaves,thus fulfilling Koch's postulates.Moreover,compared to the control treatments,inoculation with HW7-1 caused flowering delay and seed yield reduction on Tayou 2 with production of microsclerotia on the stems.To our knowledge,this is the first report of V.longisporum lineage A1/D3 on rapeseed in northwestern China.
基金supported by the National Natural Science Foundation of China (41971371)the National Key Research and Development Program of China (2022YFB3903504)the Fundamental Research Funds for the Central Universities,China (CCNU22JC022)。
文摘Large-scale crop mapping using remote sensing data is of great significance for agricultural production, food security and the sustainable development of human societies. Winter rapeseed is an important oil crop in China that is mainly distributed in the Yangtze River Valley. Traditional winter rapeseed mapping practices are insufficient since they only use the spectral characteristics during the critical phenological period of winter rapeseed, which are usually limited to a small region and cannot meet the needs of large-scale applications. In this study, a novel phenology-based winter rapeseed index(PWRI) was proposed to map winter rapeseed in the Yangtze River Valley. PWRI expands the date window for distinguishing winter rapeseed and winter wheat, and it has good separability throughout the flowering period of winter rapeseed. PWRI also improves the separability of winter rapeseed and winter wheat, which traditionally have been two easily confused winter crops. A PWRI-based method was applied to the Middle Reaches of the Yangtze River Valley to map winter rapeseed on the Google Earth Engine platform. Time series composited Sentinel-2 data were used to map winter rapeseed with 10 m resolution. The mapping achieved a good result with overall accuracy and kappa coefficients exceeding 92% and 0.85, respectively. The PWRI-based method provides a new solution for high spatial resolution winter rapeseed mapping at a large scale.
基金supported by the National Natural Science Foundation of China(31871653 to K.L.,31830067 to J.L.)the Talent Project of Chongqing Natural Science Foundation(cstc2021ycjhbgzxm0033 to K.L.)Germplasm Creation Special Program of Southwest University.
文摘Rapeseed(Brassica napus)is an oil crop grown worldwide,making it a key plant species in molecular breeding research.However,the complexity of its polyploid genome increases sequencing costs and reduces sequencing accuracy.Target capture coupled with high-throughput sequencing is an efficient approach for detecting genetic variation at genomic regions or loci of interest.In this study,588 resequenced accessions of rapeseed were used to develop a target capture sequencing SNP genotyping platform named BnaPan50T.The platform comprised 54,765,with 54,058 resequenced markers from the pan-genome,and 855 variant trait-associated markers for 12 agronomic traits.The capture quality of BnaPan50T was demonstrated well in 12 typical accessions.Compared with a conventional genotyping array,BnaPan50T has a high SNP density and a high proportion of SNPs in unique physical positions and in annotated functional genes,promising wide application.Target capture sequencing and wholegenome resequencing in 90 doubled-haploid lines yielded 60%specificity,78%uniformity within tenfold coverage range,and 93%genotyping accuracy for the platform.BnaPan50T was used to construct a genetic map for quantitative trait loci(QTL)mapping,identify 21 unique QTL,and predict several candidate genes for yield-related traits in multiple environments.A set of 132 core SNP loci was selected from BnaPan50T to construct DNA fingerprints and germplasm identification resources.This study provides genomics resources to support target capture sequencing,genetic analysis and genomic breeding of rapeseed.
基金supported by the National Key R&D Program of China(2021YFD1600502).
文摘Rapeseed(Brassica napus L.)harvesting method is critical since it significantly determines the seed yield,oil quality,and industrial efficiency.This study investigated the influences of harvesting methods on the quality of cold-pressed rapeseed oil of two varieties.Oil color,peroxide value(POV),tocopherol content,fatty acid composition,and polarity of total polyphenols(PTP)contents of two rapeseed varieties in Huanggang and Xiangyang were compared through artificially simulated combined harvesting and two-stage harvesting.Results showed significant differences in the quality of rapeseed oil between the two harvesting methods.The red value(R-value),POV,total tocopherol contents,linoleic and linolenic acid content,and PTP content of the pressed rapeseed oil prepared by the combined harvesting method were about 27.6,5.7,15.8,2.0,0.5,and 28.6%lower than those of the oil produced from the two-stage harvesting method,respectively.Xiangyang and Huayouza62 performed better in the two regions and two varieties,respectively.To sum up,the rapeseed oil obtained 41–44 days after final flowering of combined harvesting,35 days after final flowering,and six days of post-ripening of the two-stage harvesting had the best quality.
基金Supported by Youth Project of the National Social Science Fund of China(22CMZ015).
文摘Hubei Province is the main winter rapeseed producing area in China,and has the national advantage and characteristic industrial cluster of rape.The protection zone for the production of important agricultural products of rapeseed in Hubei Province also ranks first as that in Hunan Province.This paper studied the winter rape industry,the important agricultural product production protection zone of rapeseed,the provincial double-low high-quality rape protection zone and the industrial cluster of rape in Jianghan Plain in Hubei Province,and analyzed the main problems existing in the development of rapeseed production protection zone and industrial cluster of rape.Finally,it came up with strategies including promoting national rape varieties,overcoming the problem of rape"agricultural chip",promoting the"rice+rape"rotation model,and strengthening the protection of intellectual property rights of brand marks.
文摘The statistics of the number of rapeseed seedlings are very important for breeders and planters to conduct seed quality testing,field crop management and yield estimation.Calculating the number of seedlings is inefficient and cumbersome in the traditional method.In this study,a method was proposed for efficient detection and calculation of rapeseed seedling number based on improved you only look once version 5(YOLOv5)to identify objects and deep-sort to perform object tracking for rapeseed seedling video.Coordinated attention(CA)mechanism was added to the trunk of the improved YOLOv5s,which made the model more effective in identifying shaded,dense and small rapeseed seedlings.Also,the use of the GSConv module replaced the standard convolution at the neck,reduced model parameters and enabled it better able to be equipped for mobile devices.The accuracy and recall rate of using improved YOLOv5s on the test set by 1.9%and 3.7%compared to 96.2%and 93.7%of YOLOv5s,respectively.The experimental results showed that the average error of monitoring the number of seedlings by unmanned aerial vehicles(UAV)video of rapeseed seedlings based on improved YOLOv5s combined with depth-sort method was 4.3%.The presented approach can realize rapid statistics of the number of rapeseed seedlings in the field based on UAV remote sensing,provide a reference for variety selection and precise management of rapeseed.
基金This work was finically supported by the National Natural Science Foundation of China(Nos.30800767 and 31271979)the Opening Foundation of Large-scale Equipment in Tianjin University.
基金supported by the Agricultural Science and Technology Innovation Program of CAAS,the China Agriculture Research System(CARS-13)
文摘Rapeseed(Brassica napus L.) is the largest oilseed crop in China and accounts for about 20% of world production.For the last 10 years,the production,planting area,and yield of rapeseed have been stable,with improvement of seed quality and especially seed oil content.China is among the leading countries in rapeseed genomic research internationally,having jointly with other countries accomplished the whole genome sequencing of rapeseed and its two parental species,Brassica oleracea and Brassica rapa.Progress on functional genomics including the identification of QTL governing important agronomic traits such as yield,seed oil content,fertility regulation,disease and insect resistance,abiotic stress,nutrition use efficiency,and pod shattering resistance has been achieved.As a consequence,molecular markers have been developed and used in breeding programs.During 2005–2014,215 rapeseed varieties were registered nationally,including 210 winter-and 5 spring-type varieties.Mechanization across the whole process of rapeseed production was investigated and operating instructions for all relevant techniques were published.Modern techniques for rapeseed field management such as high-density planting,controlled-release fertilizer,and biocontrol of disease and pests combined with precision tools such as drones have been developed and are being adopted in China.With the application of advanced breeding and production technologies,in the near future,the oil yield and quality of rapeseed varieties will be greatly increased,and more varieties with desirable traits,especially early maturation,high yield,high resistance to biotic and abiotic stress,and suitability for mechanized harvesting will be developed.Application of modern technologies on the mechanized management of rapeseed will greatly increase grower profit.
基金granted by the Earmarked Fund for Modern Agro-industry Technology Research System of China(CARS-36)Feed Biotechnology Project of Sichuan Province of China with grant number 2010GZ0193
文摘Background: Rapeseed cake is a good source of protein for animal feed but its utilization is limited due to the presence of anti-nutritional substances, such as glucosinolates (GIs), phytic acid, tannins etc. In the present study, a solid state fermentation (SSF) using Aspergillus niger was carried out with the purpose of degrading glucosinolates and improving the nutritional quality of rapeseed cake (RSC). The effects of medium composition and incubation conditions on the GIs content in fermented rapeseed cake (FRSC) were investigated, and chemical composition and amino acid in vitro digestibility of RSC substrate fermented under optimal conditions were determined. Results: After 72 h of incubation at 34℃, a 76.89% decrease in GIs of RSC was obtained in solid medium containing 70% RSC, 30% wheat bran at optimal moisture content 60% (w/w). Compared to unfermented RSC, trichloroacetic acid soluble protein (TCA-SP), crude protein and ether extract contents of the FRSC were increased (P〈 0.05) 103.71, 23.02 and 23.54%, respectively. As expected, the contents of NDF and phytic acid declined (P〈 0.05) by 9.12 and 44.60%, respectively. Total amino acids (TAA) and essential amino acids (EAA) contents as well as AA in vitro digestibility of FRSC were improved significantly (P 〈 0.05). Moreover, the enzyme activity of endoglucanase, xylanase, acid protease and phytase were increased (P 〈 0.05) during SSF. Conclusions: Our results indicate that the solid state fermentation offers an effective approach to improving the quality of proteins sources such as rapeseed cake.
基金supported by the Modern Agricultural Industry Technology System(CARS-36)Developing key equipment for digital management and monitoring environment in animal production(2013AA10230602)+1 种基金National Natural Science Foundation of China(31372317)the 111 Project(B16044)
文摘Background: Two experiments were conducted to estimate the net energy(NE) of corn, soybean meal, expel er-pressed rapeseed meal(EP-RSM) and solvent-extracted rapeseed meal(SE-RSM) using indirect calorimetry and to validate the NE of these four ingredients using pig growth performance.Methods: In Exp.1, 24 barrows(initial BW = 36.4 ± 1.6 kg) were allotted to 1 of 4 diets which included a corn basal diet,a corn-soybean meal basal diet and two rapeseed meal diets containing 20% EP-RSM(9.5% ether extract) or SE-RSM(1.1% ether extract) substituted for corn and soybean meal. The design allowed the calculation of NE values of corn,soybean meal and rapeseed meals according to the difference method. In Exp.2, 175 growing pigs(initial BW = 36.0± 5.2 kg) were fed 1 of 5 diets for 28 d, with five pigs per pen and seven replications(pens) per treatment in order to validate the measured energy values. Diets were a corn-soybean meal diet and four diets including 10% or 20% EP-RSM and 10% or 20% SE-RSM.Results: The NE of corn, soybean meal, EP-RSM and SE-RSM were 12.46, 11.34, 11.71 and 8.83 MJ/kg DM, respectively. The NE to ME ratio of corn(78%) was similar to tabular values, however, the NE to ME ratios of soybean meal(70%) and rapeseed meal(76%) were greater than tabular values. The greater NE value in EP-RSM than in SE-RSM is consistent with its higher EE content. Increasing EP-RSM or SE-RSM did not affect the growth performance of pigs and the caloric efficiency of NE was comparable for al diets.Conclusions: The NE of EP-RSM was similar to soybean meal, and both were greater than SE-RSM. The DE, ME and NE values measured in Exp.1 are confirmed by results of Exp. 2 with comparable caloric efficiencies of DE, ME or NE for all diets.
基金supported by the National Natural Science Foundation of China(31671731)the National Key Research and Development Program of China(2016YFD0101300)+2 种基金the China Agricultural Research System(CARS-13)the Natural Science Foundation of Jiangsu Province,China(BK20151369)the Science Foundation of Jiangsu Academy of Agricultural Sciences,China(6111618)
文摘Rapeseed is a very important oil crop in China; however, its production is challenging due to the absence of effective weed management strategies. This is predominantly because of a shortage of herbicide resistance genes. Acetohydroxyacid synthase (AHAS) herbicides inhibit AHAS, a key enzyme involved in branched-chain amino acid synthesis that is required for plant growth. A rapeseed line designated M342 with AHAS herbicide resistance was developed through seed muta- genesis and was studied to assess the level and mode of inheritance of the resistance and to identify the molecular basis of resistance. M342 possessed a high level of cross-resistance to sulfonylureas (SUs) and imidazolinones (IMIs). This resistance was due to AHAS insensitivity to these herbicides and was inherited as a dominant trait conferred by a single nuclear-encoded gene. Molecular analysis revealed the presence of a Trp574Leu mutation in M342, and an allele-specific cleaved amplified polymorphic sequence (AS-CAPS) marker was developed and cosegregated with herbicide resistance in the F2, BC1, and BC2 populations. This mutation altered the transcript levels of BnAHAS1 and BnAHAS3 in M342 compared with those in the wild type, but it did not affect the agronomic or quality traits. The simple genetic inheritance of this mutation and the availability of the cleaved amplified polymorphic sequence (CAPS) marker and herbicide resistance gene should facilitate the development of herbicide-resistant rapeseed cultivars for effective weed control in China.
基金Funds were provided by the National Key Research and Development Program of China (2018YFD1000900)
文摘Strigolactones(SLs)are newly discovered plant hormones which regulate the normal development of different plant organs,especially root architecture.Lateral root formation of rapeseed seedlings before winter has great effects on the plant growth and seed yield.Here,we treated the seedlings of Zhongshuang 11(ZS11),an elite conventional rapeseed cultivar,with different concentrations of GR24(a synthetic analogue of strigolactones),and found that a low concentration(0.18μmol L–1)of GR24 could significantly increase the lateral root growth,shoot growth,and root/shoot ratio of seedlings.RNA-Seq analysis of lateral roots at 12 h,1 d,4 d,and 7 d after GR24 treatment showed that 2301,4626,1595,and 783 genes were significantly differentially expressed,respectively.Function enrichment analysis revealed that the plant hormone transduction pathway,tryptophan metabolism,and the phenylpropanoid biosynthesis pathway were over-represented.Moreover,transcription factors,including AP2/ERF,AUX/IAA,NAC,MYB,and WRKY,were up-regulated at 1 d after GR24 treatment.Metabolomics profiling further demonstrated that the amounts of various metabolites,such as indole-3-acetic acid(IAA)and cis-zeatin were drastically altered.In particular,the concentrations of endogenous IAA significantly decreased by 52.4 and 75.8%at 12 h and 1 d after GR24 treatment,respectively.Our study indicated that low concentrations of exogenous SLs could promote the lateral root growth of rapeseed through interaction with other phytohormones,which provides useful clues for the effects of SLs on root architecture and crop productivity.
基金the National Natural Science Foundation of China(31671616)the China Agriculture Research System(CARS-12)the Fundamental Research Funds for the Central Universities(2662019PY076)。
文摘Biological yield indicates the potential for increasing yield.Leaf carbon metabolism plays an important role in the biomass accumulation of rapeseed(Brassica napus L.).Field experiments with the hybrid HZ62(with a conventional plant architecture)grown in 2016–2017,and HZ62 and accession 1301(with a compact plant architecture)grown in 2017–2018 were conducted to characterize the physiological and proteomic responses of leaf photosynthetic carbon metabolism to density and row spacing configurations.The densities were set at 15×10;ha^(-1)(D1),30×10^(4)ha^(-1)(D2),and 45×10^(4)ha^(-1)(D3)(main plot),with row spacings of 15 cm(R15),25 cm(R25),and 35 cm(R35)(subplot).Individual and plant population biomass accumulation was greatest at R25,R15,and R15 for D1,D2,and D3,respectively,for both genotypes.In comparison with D1 R25,the individual aboveground biomass of HZ62 decreased by60.2%,whereas the population biomass increased by 31.9%,and the individual biomass of genotype1301 decreased by 54.0%and the population biomass increased by 53.9%at D3 R15.Leaf carbon metabolic enzymes varied between genotypes at flowering stage.In contrast to D1 R25,at D3 R15 the activities of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)and sucrose phosphate synthase(SPS)and the contents of starch,sucrose and soluble sugars in leaves were significantly decreased in HZ62 and increased in genotype 1301.The activities of fructose-1,6-bisphosphatase(FBPase)decreased,in consistency with the abundance of fructose-bisphosphate aldolase in HZ62.In contrast,sucrose synthase(Su Sy)activity appeared to decrease in both genotypes,but a significant increase in abundance of a protein with sucrose synthase was found in the 1301 genotype by proteomic analysis.With increased density and reduced row spacing,the expression of most key proteins involved in carbon metabolism was elevated,and enzyme activity and carbon assimilate content were increased in 1301,whereas HZ62 showed the opposite trend,indicating that the compact plant type can accumulate more population biomass with denser planting.
基金supported by the National Basic Research Program of China (973 Program, 2011CB109302)the National High-Tech R&D Program of China (863 Program,2011AA10A104)the earmarked fund for China Agriculture Research System (CARS-13)
文摘Pod shatter resistance of rapeseed is of great importance for modem farming practice. In order to determine the combining ability of elite inbred lines and the breeding potential of rapeseed hybrids in terms of pod shatter resistance, analysis of a 6×6 incomplete diallel cross was conducted at two locations. Results showed that a significant variation existed among breeding lines and their F1 hybrids for pod shatter resistant index (SRI), pod length and width. Pod shatter resistance was significantly positively correlated with pod length. The general combining ability (GCA) effects (GCA=l.58) played a more important role than specific combining ability (SCA) effects (SCA=0.20) for pod shatter trait. The elite lines R1, 1019B and 1055B displayed significant positive GCA effects for pod shatter resistance. Four crosses (1019B×R1, 1015B×R1, 6098B×R1, and 8908B×R1) with high mean performance and positive SCA effects were recommended for developing new hybrids for mechanical harvest in the middle reaches of the Yangtze River.
基金financially supported by the Modern Agricultural Industry Technology System(CARS-36)Special Public Sector Fund in Agriculture (200903006)
文摘Background: The nutritional value of rapeseed meal may be variable due to the variation of its chemical composition. And a precise understandin9 of the nutritional value of an ingredient is beneficial for the accurate diet formulation and reduction of feed costs. This study was conducted to determine the chemical composition, dicjestible energy (DE) and metabolizable enercjy (ME) content, and apparent ileal digestibility (AID) and standardized ileal dicjestibility (SID) of amino acids (AA) for growincj pigs. Thirteen solvent-extracted double-low rapeseed meal (DLIRSM) samples were obtained from the main double-low rapeseed producing areas in China. Methods: The DE and ME contents of the 13 DLRSM samples were measured in cjrowin9 pigs (six pigs per DLRSM sample, average initial body weicjht (BW) =48.3 kg). The AID and SID of AA of 10 DLRSM samples were determined in 12 crossbred barrows (average initial BW = 35.3 kcj) by using two 6 x 6 Latin square designs. Each Latin square comprised one N-flee diet and 5 DLRSM test diets. Results: The chemical composition of DLRSM varied among samples, and the coefficient of variation was greater than ]0 % for ether extract (EE), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium (Ca), and total 91ucosinolates. The AA content of DLRSM varied among samples especially for lysine (Lys) and methionine (Met). On a dry matter (DM) basis, the apparent total tract digestibility (ATFD) of gross energy (GE), the DE and ME and the ME:DE ratio of DLRSM averaged 62.39 %, 2862 kcal/k9 and 2723 kcal/kcj, and 94.95 %, respectively. The mean value of S1D of Lys was 70.52 % which varied from 66.54-76.54 %. The SID of crude protein (CP), Met, and threonine (Thr) averaged 72.81%, 82.41%, and 69.76 %, respectively. Conclusions: There was great variability in chemical composition especially in the concentration of EE, NDF and ADF, but no significant differences in energy content of the DLIRSM samples were observed. In addition, the AID and SID of all AA were relatively similar amoncj DLRSM samples except for that of Lys.
基金supported by the National Key Research and Development Program of China(2018YFD0200900)the earmarked fund for China Agriculture Research System(CARS-12)+1 种基金the Fundamental Research Funds for the Central Universities,China(2662017JC010)the National Project of Soil Testing and Fertilizer Recommendation,China support of the large-scale field experiments。
文摘A yield gap analysis for rapeseed(Brassica napus L.) is critical to meeting the oil demand by identifying yield potential and yield constraints. In this study, potential yield(Y_p), attainable yield(Y_(att)), and actual yield(Y_(act)) for winter rapeseed were determined in five different zones of China. A boundary line approach was adopted to calculate Y_p, based on a large-scale field experimental database. A meta-analysis was conducted on the data obtained from 118 published studies to evaluate the effects of agronomic factors on rapeseed yield. The main results indicated that farmers only achieved 37–56% of the yield potential across the zones. The low altitude areas(L-URY) and lower reaches(LRY) of the Yangtze River Basin(YRB), China had high yield levels. The total yield gap was 1 893 kg ha^(–1), due to the agronomic management factors, environmental factors, and socioeconomic factors. The meta-analysis showed that weed control and drainage were the best management practices to improve yields(45.6 and 35.3%, respectively), and other practices improved yields by 17.1–21.6%. Consequently, to narrow the yield gap over the short term, the study could focus on techniques that are easily implemented to farmers.
基金funded by the National Natural Science Foundation of China (42061144003)。
文摘The timely and rapid mapping of rapeseed planting areas is desirable for national food security. Most current rapeseed mapping methods depend strongly on images with good observations obtained during the flowering stages. Although vegetation indices have been proposed to identify the rapeseed flowering stage in some areas, automatically mapping rapeseed planting areas in large regions is still challenging.We developed an automatic phenology-and pixel-based algorithm(APPA) by integrating Landsat 8 and Sentinel-1 satellite data. We found that the Normalized Rapeseed Flowering Index shows unique spectral characteristics during the flowering and post-flowering periods, which distinguish rapeseed parcels from other land-use types(urban, water, forest, grass, maize, wheat, barley, and soybean). To verify the robustness of APPA, we applied APPA to seven areas in five rapeseed-producing countries with flowering images unavailable. The rapeseed maps by APPA showed consistently high accuracies with producer accuracies of 0.87–0.93 and F-scores of 0.92–0.95 based on 4503 verification samples. They showed high spatial consistency at the pixel level with the land cover Scientific Expertise Centres(SEC) map in France,Crop Map of England in United Kingdom, national-scale crop-and land-cover map of Germany, and Annual Crop Inventory in Canada at the pixel level. We propose APPA as a highly promising method for automatically and efficiently mapping rapeseed areas.
基金supported by the National Natural Science Foundation of China(31870519,31901503 and 31671731)the National Key Research and Development Program of China(2016YFD0101300 and 2016YFD0100202-10)+1 种基金the China Agriculture Research System(CARS-12)the Natural Science Foundation of Jiangsu Province(BK20190267)。
文摘With the increasing promotion of simplified rapeseed cultivation in recent years,the development of cultivars with high resistance to herbicides is urgently needed.We previously developed M342,which shows sulfonylurea herbicide resistance,by targeting acetohydroxyacid synthase(AHAS),a key enzyme in branched-chain amino acid synthesis.In the present study,we used a progeny line derived from M342 for an additional round of ethyl methane sulfonate mutagenesis,yielding the novel mutant DS3,which harbored two mutations in AHAS genes and showed high sulfonylurea resistance.One mutation was the substitution Trp574 Leu,as in M342,according to Arabidopsis protein sequencing.The other site was a newly recognized substitution,Pro197 Leu.A KASP marker targeting Pro197 Leu was developed and reliably predicted the response to sulfonylurea herbicides in the F2 population.The combination of Trp574 Leu and Pro197 Leu in DS3 produced a synergistic effect that greatly increased herbicide resistance.Analysis of the protein structures of AHAS1 and AHAS3 in wild-type and single-gene mutant plants revealed three-dimensional protein conformational changes that could account for differences in herbicide resistance characteristics including toxicity tolerance,AHAS enzyme activity,and AHAS gene expression.
基金financially supported by the Key R&D Program of Jiangxi Province of China (20152ACF60010)the Key Projects of Hunan Science and Technology Support Program of China (Application research of Guiye A male sterile line)+1 种基金the Research Project of Humanities and Social Sciences in Universities of Jiangxi Province, China(JC1315)supported by Emmy Noether DFG grant MA 6473/1-1
文摘financially supported by the Key R&D Program of Jiangxi Province of China (20152ACF60010);the Key Projects of Hunan Science and Technology Support Program of China (Application research of Guiye A male sterile line);the Research Project of Humanities and Social Sciences in Universities of Jiangxi Province, China(JC1315);supported by Emmy Noether DFG grant MA 6473/1-1
基金supported by the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (CAAS)the Hubei Agricultural Science and Technology Innovation Center, Chinathe Canola Key Industrial Innovation Team of Xiaogan, China
文摘Melatonin is a naturally occurring compound in plants. Here, we tested the effect of exogenous melatonin on rapeseed(Brassica napus L.) grown under salt stress. Application of 30 μmol L^-1 melatonin alleviated salt-induced growth inhibition, and the shoot fresh weight, the shoot dry weight, the root fresh weight, and the root dry weight of seedlings treated with exogenous melatonin increased by 128.2, 142.9, 122.2, and 124.2%, respectively, compared to those under salt stress. In addition, several physiological parameters were evaluated. The activities of antioxidant enzymes including peroxidase(POD), catalase(CAT) and ascorbate peroxidase(APX) were enhanced by 16.5, 19.3, and 14.2% compared to their activities in plants without exogenous melatonin application under salt stress, while the H2O2 content was decreased by 11.2% by exogenous melatonin. Furthermore, melatonin treatment promoted solute accumulation by increasing the contents of proline(26.8%), soluble sugars(15.1%) and proteins(58.8%). The results also suggested that higher concentrations(〉50 μmol L^-1) of melatonin could attenuate or even prevent the beneficial effects on seedling development. In conclusion, application of a low concentration of exogenous melatonin to rapeseed plants under salt stress can improve the H2O2-scavenging capacity by enhancing the activities of antioxidant enzymes such as POD, CAT and APX, and can also alleviate osmotic stress by promoting the accumulation of osmoregulatory substances such as soluble proteins, proline, and water soluble glucan. Ultimately, exogenous melatonin facilitates root development and improves the biomass of rapeseed seedlings grown under salt stress, thereby effectively alleviating the damage of salt stress in rapeseed seedlings.