Antibody Production for a Rapid Fluorescence Polarization Immunoassay of Estrone

Estrone has been identified as a potential endocrine-disrupting chemical （EDC）[1]. Estrone is usually quantified by gas chromatography-mass spectrometry （GC-MS）, GC-MS/MS, high performance liquid chromatography （HPLC）, HPLC- MS, and HPLC-MS/MS, etc.[2-3]. Meanwhile, several immunoassays based on radioimmunoassay, enzyme linked immunosorbent assay （ELISA） or chemiluminescence immunoassay （CLIA） for determination of estrone in real samples have been developed[2＇4]. Although these methods are sensitive, they need multistage separation and are thus time-consuming and laborious. A very promising way for the simplification of immunoassays for routine applications is a shift from heterogeneous methods （with separation） to homogeneous assays （without separation）[5]. Fluorescence polarization immunoassay （FPIA） is one of the homogeneous techniques that meets the requirements of a simple, reliable, fast, and cost-effective analysis[6]. Therefore, the present study is focused on the development of FPIA in order to analyze estrone based on antibody production.

STUDY ON MICROWAVE DIELECTRIC PROPERTIES OF EPOXY RESIN MIXTURES USED FOR RAPID PRODUCT DEVELOPMENT

This paper presents a preliminary study on the dielectric properties and curing of three different types of epoxy resins mixed at various stoichiometric mixture of hardener, flydust and aluminium powder under microwave energy. In this work, the curing process of thin layers of epoxy resins using microwave radiation was investigated as an alternative technique that can' be implemented to develop a new rapid product development technique. In this study it was observed that the curing time and temperature were a function of the percentage of hardener and fillers presence in the epoxy resins. Initially dielectric properties of epoxy resins with hardener were measured which was directly correlated to the curing process in order to understand the properties of cured specimen. Tensile tests were conducted on the three different types of epoxy resins with hardener and fillers. Modifying dielectric properties of the mixtures a significant decrease in curing time was observed. In order to study the microstructural changes of cured specimen the morphology of the fracture surface was carried out by using scanning electron microscopy.

Rapid Sample Product Trial Manufacture Technique for Developing Water Meter New Products

This paper advances the viewpoints and methods of the rapid sample product trial manufacture technique for developing water meter new products by CAD and simulation, computer virtual assembling and optimizing, rapid machining process and measurement etc. as the design and sample product trial manufacture process of water meter new products are long in product development period, and low in product development efficiency in the present time.

Online Fashion Retailer Bonobos Selects Gerber Technology's YuniquePLM^(TM) Industry-Tailored Software to Support Rapid Growth and Fast-Paced Product Innovation

Tolland,Connecticut,USA-Gerber Technology,a business unit of Gerber Scientific, Inc.(NYSE:GRB) and a world leader in automated CAD/CAM and PLM solutions for the apparel and flexible materials industry,announces that fast-growing internet retailer Bonobos,Inc. has selected the YuniquePLM product

Integrated Product and Process Design Using a Reverse Engineering System

Computer-integrated manufacturing (CIM) and revers e engineering (RE) have changed drastically the concept of product re-design, pla nning and manufacture of components. However, the main problems currently facing the developers of reverse engineering system, is the time consuming digitis ation of 3D data and the conversion of large amounts of data into a concise and manageable format and linking it to a CAD/CAM system. Automated 3-D profile gen eration, measurements and inspection of manufactured component represents one of the important functions in reverse engineering and in the improvements in produ ct quality in rapid product developments. The paper presents a novel methodology for the development of a reverse enginee ring technique for use in the rapid product development in a CIM environment . The system developed provides integration, data capture and manipulation, dat a transfer between a CAD, CAM, Computer-aided inspection (CAI) and a 3-D profi le scanning system. An efficient scanning strategy has been developed for scann ing and surfaces data acquisition. The products were scanned using a laser scann ing system with a dedicated scan control card and the associated software packag es. A versatile rig was developed for the ease of data gathering of the profile scanning process. The surface data collected was then used to build a mathematic al surface model, which was then used to develop a virtual 3-D model of the pro duct. The resulting surface model provides the geometrical input to the subseque nt machining operation using either a CNC system or other manufacturing operatio n e.g. dies casting/mould casting etc. The prototypes developed were inspected u sing a state-of-the art CNC-CMM that was integrated to the CAD/CAM system. Si nce the scanning/digitised data captured by laser scanning probes requires no ma nual editing, significant time saving over most non-contact probe systems was a chieved. Since the creation of an accurate CAD model of a redesigned component o r a prototype constitute a major element of the total turnaround time; maximum r eturn can be achieved by increasing the efficiency of the redesigning process. T he paper also outlines with a case study the application of the developed system . The system developed offers the flexibility of using the concept of reverse en gineering of a variety of components with the complimentary facility of integrat ion between CAD/CAM Computer-aided Inspection (CAI) systems and a scanning syst em. The developed reverse engineering application in an integrated manufacturing system can increase the consistency, improve cost-efficiency, reduce produ ct turn around and skill levels required to redesign, reengineer and prototyping components and products.

Rapid Prototyping and Manufacturing Technology and Its Integration with CIMS

Recent developments of rapid prototyping and manufaturing (RP&M)technology are discussed. To facilitate application of RP&M technology as an enabling technology in product development and manufacturing, our center has done a series of pojects covering RP theory (modern shaping science), new RP processes and equipment,rapid tooling technology and rapid product development systems. With the STEP protocol and product modeling technology, RP&M technology can be integrated into CIMS to form a new subsystem, the free form manufacturing subsystem. The subsystem architecture is investigated in this paper.