A novel electrochemical immunoassay for cardiac troponin Ⅰ （cTnI） combining the concepts of the dual monoclonal antibody ＂sandwich＂ principle, the silver enhancement on the nano-gold particle, and the SBA-15 meso...A novel electrochemical immunoassay for cardiac troponin Ⅰ （cTnI） combining the concepts of the dual monoclonal antibody ＂sandwich＂ principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode （SBA-MCPE） is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.展开更多
Dear Editor,Influenza A viruses(IAVs)are single-stranded,negative sense RNA viruses.IAV subtype is determined on the basis of the viral surface glycoproteins,hemagglutinin(HA),and neuraminidase(NA).To date,18 HA and 1...Dear Editor,Influenza A viruses(IAVs)are single-stranded,negative sense RNA viruses.IAV subtype is determined on the basis of the viral surface glycoproteins,hemagglutinin(HA),and neuraminidase(NA).To date,18 HA and 11NA subtypes have been reported(Tong et al.,2012).展开更多
The aims of this study are to use quantitative analysis of the prostate-specific antigen(PSA)in the seminal stain examination and to explore the practical value of this analysis in forensic science.For a comprehensive...The aims of this study are to use quantitative analysis of the prostate-specific antigen(PSA)in the seminal stain examination and to explore the practical value of this analysis in forensic science.For a comprehensive analysis,vaginal swabs from 48 rape cases were tested both by a PSA fluorescence analyzer(i-CHROMA Reader)and by a conventional PSA strip test.To confirm the results of these PSA tests,seminal DNA was tested following differential extraction.Compared to the PSA strip test,the PSA rapid quantitative fluorescence analyzer provided the more accurate and sensitive results.More importantly,individualized schemes based on quantitative PSA results of samples can be developed to improve the quality and procedural efficiency in the forensic seminal inspection of samples prior to DNA analysis.展开更多
文摘A novel electrochemical immunoassay for cardiac troponin Ⅰ （cTnI） combining the concepts of the dual monoclonal antibody ＂sandwich＂ principle, the silver enhancement on the nano-gold particle, and the SBA-15 mesoporous modified carbon paste electrode （SBA-MCPE） is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnI from 0.5 to 5.0 ng/mL and a limit of detection of 0.2 ng/mL of cTnI were obtained.
基金partially supported by the National Institutes of Health(grant no.P20GM103646)the United States Department of Agriculture Animal and Plant Health Inspection Service(agreement 14-7428-1041-CA)
文摘Dear Editor,Influenza A viruses(IAVs)are single-stranded,negative sense RNA viruses.IAV subtype is determined on the basis of the viral surface glycoproteins,hemagglutinin(HA),and neuraminidase(NA).To date,18 HA and 11NA subtypes have been reported(Tong et al.,2012).
基金support from the Program for Young Innovative Research Team in China University of Political Science and Law.
文摘The aims of this study are to use quantitative analysis of the prostate-specific antigen(PSA)in the seminal stain examination and to explore the practical value of this analysis in forensic science.For a comprehensive analysis,vaginal swabs from 48 rape cases were tested both by a PSA fluorescence analyzer(i-CHROMA Reader)and by a conventional PSA strip test.To confirm the results of these PSA tests,seminal DNA was tested following differential extraction.Compared to the PSA strip test,the PSA rapid quantitative fluorescence analyzer provided the more accurate and sensitive results.More importantly,individualized schemes based on quantitative PSA results of samples can be developed to improve the quality and procedural efficiency in the forensic seminal inspection of samples prior to DNA analysis.
