Effect of Liangxuehuayu Recipe on hemorheology in rats with blood stasis syndrome

Objective:To investigate the effects of Uangxuehuayu Recipe on hemorheology in rats with blood stasis syndrome induced by mutifactor stimuli.Methods:SD rats were divided into control, model,Uangxuehuayu Recipe(high,middle and low dose,18,9,4.S g/kg accordingly).Except the control group,blood stasis model was established in the rest groups.The hemorheological parameters were measured and compared.Results:Blood viscosity at high,moderate and low level in rats with blood stasis significantly increased(P【0.05),but blood viscosity at high level and plasma viscosity was significantly decreased in rats induced by some stimuli after Uangxuehuayu Recipe were intra-gastrically administered for I weeks(P【0.01,P【0.05). Conclusions:Uangxuehuayu Recipe is effective in improving hemorheology,and has important application value in the prevention of occurrence and development of ischemic stroke.

PNPLA5-knockout rats induced by CRISPR/Cas9 exhibit abnormal bleeding and lipid level

Patatin-like phospholipase domain containing 5 （PNPLA5） is a neotype neutral lipase with dual activity of anabolism and catabolism in vitro and in vivo, which has a low mRNA expression level in humans and mice. PNPLA5, which is localized to lipid droplets and required for efficient autophagy by optimal initiation, has been speculated to possess triglyceride hydro- lase activity, and has been associated with low density lipoprotein cholesterol （LDL-C）. Above all, PNPLA5 is a relatively new gene, which is reported less about its biological function research, especially the function research in the rats is still blank. In this study, we examined the spatiotemporal expression profile of PNPLA5 and found that it was expressed at low levels in most organs of Sprague Dawley （SD） rats, but was present at very high levels in the skin and testes. To furth.er determine the biological function of PNPLA5 in mammals, we generated PNPLA5-knockout SD rats using the clustered regularly-interspaced short palindromic repeats （CRISPR）/Cas9 system. PNPLA5-null rats were viable, but showed a variety of phenotypic abnormalities, such as abnormal bleeding, and varied hematobiochemical parameters including increased serum total cholesterol （TC）, tdglycerides and high density lipoprotein cholesterol （HDL-C） level, and reduced LDL-C level, compared with wild-type control rats. These data are consistent with an important role for PNPLA5 in lipid metabolism, provJdJng a new target gene and animal model for treatment of cardiovascular diseases in the future.

Comparative study on the difference of obesity model induced by two kinds of high fat diet in Sprague-Dawley rats

Objective: To explore the differences of obese Sprague-Dawley(SD)rats model induced by lard oil high-fat(HF)diet or purified HF diet. Methods: SD weanling rats were randomly divided into three groups: D1 group,where rats were fed by lard oil HF diet;D2 group,where rats were fed by purified HF diet;C group,where rats were fed on chow. After 12 weeks,diet-induced obesity rat(stop 33% based on weight)were selected for further study,and the rest rats from group D1 and D2 were excluded. The food intake and weight were weighted daily and weekly,respectively. The subcutaneous,visceral and total fat contents of rats was measured by 256-row CT scan and the Lee index was calculated accordingly. The kidney,liver,testis,spleen and heart were weighted respectively. Serum leptin and insulin levels were quantified. The pathology in liver and adipose tissues were analyzed by HE staining. Oral glucose tolerance test(OGTT)was used to compare the glucose tolerance ability. Serum total cholestero(lT-CHO),high density lipoprotein(HDL-C),low density lipoprotein(LDL-C),triglyceride(TG)and inflammatory cytokines IL-6,TNF-α were detected as well. Results: After 12 weeks,the body weight,subcutaneous fat,visceral fat,total fat mass,wet weight of liver,kidney and heart,area under blood glucose curve and the levels of serum insulin,leptin,T-CHO,LDL-C,TG,IL-6 and TNF-α in group D2 were significantly increased compared to those of group C and group D1. HDL-C of group D2 was markedly lower than that in group C(P<0. 05). The visceral fat,total fat content and HDL-C in group D1 were significantly different from those of group C(P<0. 05). Steatosis and enlarged adipocyte were found in the livers of rats in group D1 and D2,and the lesions were more significant in group D2. Conclusion: Purified HF diet was more effective in inducing metabolic abnormalities,steatosis,peripheral chronic inflammation in obese SD rat models. But lard oil HF diet was more economical when only inducing visceral steatosis was required.

Expression of vasa Gene in Development Process of Germ Cells in Male Rat

In order to explore the role of vasa gene in the development of germ cells after gonad differentiation in male rats,the expression of vasa mRNA and vasa protein in 17.5-day-old fetal rats and neonatal rats was detected by real-time fluorescent quantitative PCR and immunohistochemistry method.The results showed that the expression of vasa mRNA was detected in the testis tissue of 17.5-day-old fetal rats and neonatal rats,and the expression of vasa mRNA in testis of neonatal rats was high than that in fetal rats.The expression of vasa protein was detected in neonatal rats,but it was not found in fetal rats.In conclusion,vasa gene plays an important role in the development of germ cells.However,as a marker,it can only be used to label all kinds of germ cells after formation of prespermatogonia.

Changes of Serum Trace Elements, AFP, CEA, SF, T3, T4 and IGF-Ⅱ in Different Periods of Rat Liver Cancer

Objective: Based on liver cancer model built in SD rats, the contents of trace elements (Cu, Fe, Zn, Ca and Mg), AFP, CEA, SF, TH and IGF-II in serum were measured at different stages to explore the molecular changes during the rat liver cancer development. Methods: The SD rat liver cancer model was built by using diethylnitrosamine (DENA) as the mutagen. During 16 weeks of DENA gavage, blood samples were taken in the 14th, 28th, 56th, 77th, 105th and 112th days respectively after the first day of gavage with DENA, then the contents of five trace elements (Cu, Fe, Zn, Ca and Mg), T3, T4, IGF-II, AFP, CEA and SF in serum were determined. Results: During the development of the rat liver cancer, in the test group, the Cu content significantly increased in serum, while the contents of Fe, Zn and Ca significantly decreased. The content of Mg showed no significant change. AFP and CEA of the test group showed same expression level with the control group; while the content of SF was lower than that of the control group when cancerization appeared. T3 and T4 increased at the first stage and then went down, and the content of IGF-II was always high. Conclusion: Cu, Fe, Zn, Ca, T3, T4, SF and IGF-II are closely related to the development of liver cancer. The changes of their contents in the development of cancer could enlighten the researches on cancer pathogenesis and prevention.

大鼠(Rattusnorvegicus domestica)八种器官的蛋白质图谱分析

用聚丙烯酰胺凝胶电泳 (SDS -PAGE)技术分析了大鼠脑、心脏、肺、肝脏、胃、肾脏、脾脏和肌肉的蛋白质图谱 ,为以大鼠为材料的实验研究提供了一些基础资料。

Effects of Terephthalic Acid on Rat Lipid Metabolism

Objective To study the effect of terephthalic acid （TPA） on lipid metabolism in Sprague-Dawley （SD） rats. Methods Five groups of SD rats that ingested 0%, 0.04%, 0.2%, 1%, and 5% TPA, respectively, were included in a 90-day subchronic feeding study. Effects of TPA on levels of serum protein, total cholesterol （TC）, triglycerides （TG）, high-density lipoprotein cholesterol （HDL）, total antioxidative capability （T-AOC）, superoxide dismutase （SOD） and malondialdehyde （MDA） were observed. Urine samples were collected and analyzed for concentration of ion. Results TPA decreased the level of serum T-AOC in a dose dependent manner. The contents of serum and bladder MDA significantly decreased in 1% and 5% TPA ingestion groups. Serum CuZn superoxide dismutase （CuZnSOD） lowered in groups of 0.2%, 1%, and 5% TPA. TPA subchronic feeding had no significant influences on serum TC, LDL or HDL, but increased serum TG, TP and ALB after administration of 0.04% and/or 0.2% TPA. Concentrations of urinary Ca^2＋, Mg^2＋, Na^＋, and K^＋ were elevated in 1% and 5% TPA groups. Conclusion Antioxidative potential decreased after TPA exposure. MDAincrease in serum and bladder tissues was one of the most important reactions in rats which could protect themselves against TPA impairment. The decrease of serum CuZnSOD was related to the excretion of Zn^2＋.

Immune Responses to Trichloroethylene and Skin Gene Expression Profiles in Sprague Dawley Rats

Objective To characterize the immune reaction in SD rats exposed to trichloroethylene （TCE） and to identify the gene expression profiles involved in skin after TCE exposure. Methods Fifteen percent of TCE was injected intradermally into the rat back （100 μL/120 g） at intervals of 7 days. Whole blood was collected 24 h after the fifth or seventh intradermic administration of TCE. The percentages of CD4＋ and CD8＋ of T lymphocytes were measured by a flow cytometer. The concentrations of IFN-gamma and 1L-4 in the serum were semi-quantified by ELISA. Total RNAs of skin samples at 3 h or 24 h after the seventh dose of TCE in SD rats were extracted, and gene expression proftles of these tissues were analyszed by rat toxicology U34 array of Affymetrix. Results Obvious decline of CD4＋ in T lymphocytes was observed in the TCE-administer group. No significant concentration differences in IFN-gamma and IL-4 were found between TCE-treated and control rats. Gadd45a and Mel were significantly up regulated in skin tissue 24 h after TCE exposure. The expression regulation of immune response factors was as active as proteins associated with lipid metabolism and synthesis process in these skin samples of SD rats exposed to TCE. Conclusion T-helper type 1 cells mediate immune response can not be elicited in TCE-treated SD rats, but certain immune disorder can be induced.

Anatomical Study of the Intercostobrachial Nerve in Sprague-Dawlay Rats

OBJECTIVE The aim of the study was to explore the anatomy of the in-tercostobrachial nerve in rats. METHODS Dissections of 8 Sprague-dawley rats were performed to examine the intercostobrachial nerve. Fifteen dissections were successful. The position of the nerve, origin, termination, length and diameter were measured and recorded. RESULTS 1) 80% of the nerves originated from the second intercostal space and 20% from the first intercostal space; 2) 60% of the nerves terminated in the latissimus dorsi and 40% in the axillary skin 3) the branches of the nerves only were divided into 2 types, single and double; 4) the length of the nerves ranged from 2.4 to 3.4 cm with an average of 2.97±0.90 cm; 5) the diameter of the nerves was thicker at their origin compared to their termination. CONCLUSION The intercostobrachial nerve is simple in rats with 60% terminating in the latissimus dorsi.

吸入暴露途径下纳米塑料在SD大鼠体内的富集特征研究

为探讨吸入暴露纳米级塑料在生物体呼吸系统以外组织上的富集特征,以100 nm聚苯乙烯微球对SD大鼠进行2 h短期吸入暴露,使用热裂解-气相色谱质谱法测定大鼠肝脏、肾脏、心脏以及脑组织上的聚苯乙烯浓度。结果显示:暴露组大鼠肾脏、心脏和肝脏中的浓度[(0.416±0.143)、(0.633±0.278)和(0.617±0.179)g/g]显著高于对照组大鼠[(0.249±0.020)、(0.070±0.096)和(0.101±0.140)g/g],差异具有统计学意义(P<0.05),但各组织间的相关性不显著(P>0.05);2组大鼠脑组织均未检出聚苯乙烯。研究表明,100 nm聚苯乙烯微球吸入暴露2 h后,可通过肺泡进入血液循环,进而在大鼠肝脏、肾脏、心脏中形成沉积。通过探讨纳米级塑料在生物体内的累积特征,为深入开展吸入纳米塑料对人体的健康影响研究提供数据支撑。

基于液质联用法测定嘧啶核苷类化合物GY005在SD大鼠体内的药代动力学实验研究

建立LC-MS/MS法测定嘧啶核苷类化合物GY005在大鼠血浆中的浓度,并应用于大鼠体内的药代动力学研究。以特非那定为内标,经乙腈-甲醇沉淀蛋白后,采用ACE 5 C4 column(50 mm×2.1 mm,2.5μm)色谱柱,以0.1%甲酸水和0.1%甲酸乙腈为流动相,进行梯度洗脱分离,流速0.7 mL·min^(-1),进样量10μL;采用电喷雾离子源(ESI),正离子扫描方式,MRM扫描,分别以m/z 445.15→270.10和m/z 472.40→436.40作为化合物GY005和内标(特非那定)的质谱检测条件。大鼠灌服2 mg·kg^(-1)化合物GY005后,不同时间点取样测定其血浆浓度,计算药代动力学参数。雌雄SD大鼠单次静脉注射2 mg·kg^(-1)的受试品后,化合物GY005在雄性大鼠体内的暴露量(AUC_(last))为(228±44)h·ng·mL^(-1),消除半衰期(T_(1/2))为(0.30±0.01)h,清除率(CL)为(149±29)mL·min^(-1)·kg^(-1)。化合物GY005在雌性大鼠体内的暴露量(AUC_(last))为(215±158)h·ng·mL^(-1),消除半衰期(T_(1/2))为(0.32±0.03)h,清除率(CL)为(226±162)mL·min^(-1)·kg^(-1)。雌雄间暴露水平相当,没有显著性差异。这个方法具有简便、灵敏、快捷,适用于化合物GY005的药代动力学研究。

N-亚硝基布美他尼SD大鼠体内致突变性风险研究

目的评价布美他尼杂质N-亚硝基布美他尼的体内致突变风险和肝细胞DNA损伤风险。方法SD大鼠随机分为6组,分别为:溶媒对照组(溶媒为0.5%羧甲基纤维素钠),N-亚硝胺布美他尼100、300、1000 mg·kg^(-1)剂量组,阳性对照组1[N-乙基-N-亚硝基脲(ENU),40 mg·kg^(-1)]、阳性对照组2[甲磺酸乙酯(EMS),200 mg·kg^(-1)]。2个阳性对照组均为每组6只动物,其余每组12只动物。大鼠连续14 d经口灌胃给予受试物N-亚硝胺布美他尼。首次给药后约14 d和约28 d后采集外周血用于Pig-a基因突变率检测,末次给药后约3 h取肝细胞开展彗星试验。结果试验期间给予N-亚硝基布美他尼未导致异常临床症状和动物体重及摄食量改变。100、300、1000 mg·kg^(-1)剂量组动物肝细胞平均tail%DNA值(平均数/中位数)分别为2.90±0.38/2.34±0.46、3.58±0.27/2.87±0.51、3.45±0.59/2.21±1.44,与溶媒对照组相应数值相比未见差异,且无明显剂量效应相关性。首次给药后14 d,100、300、1000 mg·kg^(-1)剂量组动物平均RBC^(CD59-)和RET^(CD59-)百万分之发生率(RBC^(CD59-)百万分之发生率/RET^(CD59-)百万分之发生率)分别为2.9±1.6/1.2±0.8、2.8±2.4/1.3±1.5、2.4±1.0/1.3±1.5;首次给药后28 d,100、300、1000 mg·kg^(-1)剂量组动物RBC^(CD59-)百万分之发生率/RET^(CD59-)百万分之发生率分别为5.1±1.5/2.2±0.6、4.3±1.5/3.5±3.6、4.8±2.4/2.5±2.7。上述数值与相同时间点溶媒对照组相比均未见差异,且经统计学分析未见剂量效应相关性。结论连续经口给予N-亚硝基布美他尼14 d,SD大鼠的最大耐受量高于1000 mg·kg^(-1),未检出外周血基因突变风险和肝细胞DNA损伤风险。研究数据可为药品中遗传毒性杂质的合理监管和含N-亚硝基杂质药物的安全使用提供数据支持。

SD大鼠、小鼠创面新生皮肤组织石蜡切片的制备方法

目的:探索有效的SD大鼠、小鼠创面新生皮肤组织石蜡切片的制备方法。方法:取21只雄性SD大鼠、21只雄性SD小鼠制作创伤模型,在不同愈合时间取创面新生皮肤组织制作石蜡切片,对石蜡切片制作过程中的关键步骤进行控制,包括固定、脱水、浸蜡、包埋、切片、脱蜡、复水、染色、脱水、封藏等。比较不同愈合时间皮肤组织石蜡切片的差异。结果:实验制得组织切片染色均匀,细胞排列紧密,无裂缝,细胞核染色清晰,呈暗红色,成纤维细胞、毛囊、毛细血管和肌肉组织结构均清晰可见。随着愈合时间的延长,皮肤表皮层增厚,毛囊越来越多且分化程度提高,成纤维细胞数量增加。结论:该制备方法可以有效保证石蜡切片的质量并判定皮肤愈合程度。

SD大鼠乳鼠原代皮质神经元和小胶质细胞同时提取并培养的实验方法

背景:原代皮质神经元和小胶质细胞在探索神经系统疾病细胞疗法中起着至关重要的作用,而目前获得2种细胞的方法大多较繁琐,需分别单独提取。因此找到一种便捷、快速可同时提取2种细胞的方法十分关键。目的:探索一种新型同时提取原代皮质神经元及小胶质细胞的方法。方法:取24 h内新生SD大鼠乳鼠,将大脑取出放入装有DMEM的培养皿中,去除软脑膜后备用。同一脑组织,先提取原代神经元,再将剩余脑组织用于提取小胶质细胞,整个过程在冰上操作。原代皮质神经元提取培养步骤:镊子夹取2.0-3.0 mm厚度大脑皮质组织置于培养皿,用木瓜蛋白酶消化20 min,中止消化后吹打组织呈互相粘连的组织悬液,吸上清细胞悬液,过滤、分装到15 mL离心管中,离心并重悬后将细胞接种于左旋多聚赖氨酸包被的6孔板爬片上放入细胞培养箱,每隔1 d观察神经元形态,第7天进行MAP2和β-Tubulin免疫荧光染色鉴定。小胶质细胞提取培养步骤:夹取上一步取皮质后剩余脑组织,厚度8-10 mm,置于培养皿,用胰酶消化20 min,中止消化后吹打组织呈匀浆,然后将匀浆转移到培养瓶中培养,第14天将培养瓶封口后进行恒温水平摇床振荡2 h,小胶质细胞脱落于上清中;取纯化后的小胶质细胞继续培养3 d进行Iba1免疫荧光染色鉴定。结果与结论:(1)神经元培养24 h后贴壁、胞体变大,部分神经元长出突触;培养到第3,5天时胞体进一步增大,大部分神经元已呈突触形态,部分神经元成团生长;第7天时,神经元突触延长增粗并互相连接成网。经β-Tubulin、MAP2免疫荧光染色鉴定为神经元。(2)小胶质细胞培养第1天换液后可见细胞贴壁生长;第3,5,7天时细胞密度均较小,细胞形态呈高亮椭圆或圆形,但已基本成团块状生长于其他细胞上层;第10天时小胶质细胞密度明显增大;第14天时小胶质细胞呈致密团块状生长于其他细胞上层,此时可进行分离纯化。取分离纯化后细胞再培养至第3天,经Iba1免疫荧光鉴定为小胶质细胞,且纯度大于95%。(3)结果表明,经此法提取并培养获得的原代皮质神经元和小胶质细胞纯度高、形态好、成活率高。

不同周龄和性别的SPF级SD大鼠血液生理生化指标的测定与比较分析

目的探讨分析不同周龄和性别的SPF级SD大鼠血液生理生化指标。方法研究对象为SPF级SD大鼠80只,并随机将其分为4、12、24、48周龄4个实验组,每组内雌雄比例为1:1,所有SD大鼠均应用XFA6030型动物血液细胞分析仪对其血液生理指标展开分析,应用Beckman Coulter AU5800全自动生化分析仪对其血液生化指标展开分析。结果与12、24、48周龄SD大鼠相比较,4周龄的SD大鼠在WBC、RBC、HGB、HCT、MCV、MCH、MCHC、PLT、PCT、BA#、BA%几方面具有较大差异,P<0.05,且各项指标达到峰值的时间均为24周,在雌雄的性别差异中,4周龄SD大鼠BA%具有差异;12、24周龄SD大鼠WBC、RBC具有差异;24周龄SD大鼠BA%具有差异;48周龄SD大鼠MCH、PCT具有差异,且所有差异P值均<0.05;与12、24、48周龄SD大鼠相比较,4周龄的SD大鼠在AST、CHOL、TP、GLU、CRE、ALT、ALP、BUN、TG几方面具有较大差异,P<0.05,在雌雄的性别差异中,4周龄SD大鼠CRE、ALP见具有差异;12周龄SD大鼠AST、CHOL、BUN具有差异;12周龄以及24周龄SD大鼠ALP具有差异,且所有差异P值均<0.05。结论在性别相同但周龄不同的SD大鼠中,其血液生理生化指标存在统计学差异,同样的,在同周龄但不同性别的SD大鼠中亦存在差异。

HPLC-MS/MS法考察水黄皮素在SD大鼠体内的药代动力学特征

目的:建立大鼠血浆中水黄皮素的定量分析方法,并对其药代动力学进行研究。方法:通过灌胃给予SD大鼠水黄皮素(10 mg·kg^(-1)),血浆样品经蛋白沉淀法处理后进行HPLC-MS/MS定量分析,以五味子醇甲为内标,采用Agilent Poroshell 120 EC-C_(18)(50 mm×3.0 mm,2.7μm)色谱柱,乙腈(A)-0.1%甲酸溶液(B)为流动相,流速0.3 mL·min^(-1),梯度洗脱;采用电喷雾离子源(ESI),正离子多反应监测模式(MRM)扫描,水黄皮素[M+H]^(+)m/z 293.1→277.0,五味子醇甲[M+H]^(+)m/z433.2→384.2;使用DAS软件计算相关药代动力学参数。结果:在1.5625~1600 ng·mL^(-1)浓度范围内,水黄皮素线性关系良好(r=0.9997),定量下限为1.5625 ng·mL^(-1),精密度RSD和准确度RE均小于20%;低、中、高质控样品精密度RSD和准确度RE均小于15%,提取回收率在91.70%~100.28%,基质效应在88.35%~97.55%,稳定性RSD均小于15%。水黄皮素在雌性SD大鼠体内T_(max)、t_(1/2)、C_(max)和AUC_(0→t)分别为3 h、1.95 h、1214.85 ng·mL^(-1)和11920.43 ng·mL^(-1)·h;在雄性SD大鼠体内T_(max)、t_(1/2)、C_(max)和AUC_(0→t)分别为3 h、1.19 h、1221.57 ng·mL^(-1)和9983.40 ng·mL^(-1)·h。结论:所建方法符合生物样品定量分析的基本要求,可用于水黄皮素在大鼠血浆中的含量测定及其药代动力学评价;水黄皮素在雌性SD大鼠体内的生物利用度更高。

SPF级雄性SD大鼠回肠与结肠菌群结构比较分析

目的使用高通量测序技术分析SPF级雄性SD大鼠回肠与结肠菌群结构与丰度。方法收集30只SPF级雄性SD大鼠的回肠及结肠腔内容物,提取内容物中细菌总DNA并进行PCR扩增。使用Ilumina NovaSeq测序平台对样本中细菌16S rRNA的V3-V4区进行测序。然后基于有效数据在门、属水平上分析肠道菌群的物种结构和丰度,采用QIIME软件(扩增子)分析工具分析回肠和结肠菌群的多样性及差异,同时应用Tax4Fun程序预测回肠及结肠菌群的优势基因富集通路。结果在门水平上,雄性SD大鼠回肠内微生物优势菌群主要是厚壁菌门(Firmicutes)和放线菌门(Actinobacteria),占比超过98%;结肠内微生物优势菌群主要是厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes),占比超过95%。在属水平上,回肠以乳酸杆菌属(Lactobacillus)和另枝菌属(Alistipes)为主要优势菌群,结肠以乳酸杆菌属(Lactobacillus)及罗姆布茨菌属(Romboutsia)为优势菌群。在菌群多样性方面,结肠菌群的α多样性包括丰富度指数(Chao1指数)及多样性指数(香农指数Shannon index)都显著高于回肠(P<0.01),其菌群物种的组成结构差异性比回肠小;回肠菌群结构差异性显著的主要是厚壁菌门(Firmicutes)、罗姆布茨菌属(Romboutsia)和消化链球菌科(Peptostreptococcaceae),而结肠菌群结构差异性显著的是拟杆菌门(Bacteroidetes)、拟杆菌纲(Bacteroidia)和拟杆菌目(Bacteroidales)。在菌群功能方面,回肠菌群优势基因显著富集于类脂物代谢、多酮代谢、膜运输、生物降解等通路,而结肠菌群优势基因显著富集于甘聚糖生物合成代谢、能量代谢、辅助因子和维生素及其他产物的生物合成等通路。结论SPF级雄性SD大鼠回肠与结肠菌群在结构与丰度上存在显著差异,结肠菌群的丰富度及多样性高于回肠。

阿司匹林对SD大鼠的致畸作用

目的总结汇总我实验室2015年以来使用阿司匹林作为致畸试验阳性对照药的结果,供开展此类实验的同行们参考。方法将受孕的雌鼠随机分为阴性对照组、阿司匹林组,每组50只。受孕第6~15天灌胃给予阿司匹林280 mg/kg体重,1次/d,共10 d。受孕第20天处死孕鼠,剖腹检查受孕情况和胚胎发育情况,记录子宫连胎重、着床数、黄体数、活胎数、吸收胎数、死胎数。对胎仔进行外观检查,逐一记录胎仔体重、性别、身长、尾长。检查胎仔外观后,每窝的1/2胎仔用95%的乙醇固定,茜素红染色后进行骨骼检查,另1/2胎仔用Bouins液固定后进行内脏检查。结果与阴性对照组相比,阿司匹林组受孕大鼠GD12、GD15、GD20体重减轻,流产率增加,子宫连胎重减轻、活胎率减少、窝均活胎数减少、吸收胎率增加,胎盘重量减轻、胎仔体重减轻、胎仔身长和尾长短小,外观畸形、骨骼畸形、内脏畸形的发生率增加,差异均有统计学意义(P<0.05)。结论在本实验条件下,280 mg/kg体重的阿司匹林对SD大鼠有明显的致畸性。

菊苣复合物30 d喂养实验对SD大鼠生理状况的影响

目的:分析了解在30 d喂养实验中使用菊苣复合物对SD大鼠进行喂养并观察SD大鼠的生理状况变化。方法:选取60只体型相似的SD大鼠进行为期30 d的喂养实验,将60只大鼠每30只分为一组,雌雄各半,分为观察组和对照组。观察组采用菊苣复合物对大鼠进行喂养,对照组则采用传统喂养方式对大鼠进行喂养,观察并记录菊苣复合物对大鼠生理状况的影响。结果:在30 d动物喂养期间,菊苣复合物未引起大鼠的生理、生化功能器官以及组织形态、整体健康状况等身体的各项指标的异常变化,各项检查结果对照组传统鼠粮与菊苣复合物喂养相比较,差异均无统计学意义。结论:30 d大鼠喂养实验后,对比喂养前前后以及观察组的数据分析食用菊苣复合物对大鼠的各项生理指标有明显的改善,所以菊苣复合物喂养SD大鼠对其的生理指标有益。

食叶草营养成分分析及对SD大鼠尿酸的影响

以河北产食叶草为研究对象,系统分析食叶草中矿物质、多糖、粗纤维、蛋白质、氨基酸、多酚和黄酮的含量,及对高尿酸SD大鼠尿酸水平干预的效果。结果显示,食叶草干物质中蛋白质(32.5%)、多糖(11.00 mg/g)、多酚(5.11 mg/g)、黄酮(18.30 mg/g)、钾、钙、镁等营养物质含量丰富,氨基酸含量丰富且模式优良。食叶草高蛋白属性并未加重高尿酸模型大鼠尿酸水平。