Weather conditions play a pivotal role in embryo development and parental incubation costs,potentially impacting the clutch size and incubation behavior of birds.Understanding these effects is crucial for bird conserv...Weather conditions play a pivotal role in embryo development and parental incubation costs,potentially impacting the clutch size and incubation behavior of birds.Understanding these effects is crucial for bird conservation.Reeves’ s Pheasant(Syrmaticus reevesii) is a threatened species endemic to China,which is characterized by female-only incubation.However,there is a lack of information regarding the impact of weather conditions on clutch size and incubation behavior in this species.Using satellite tracking,we tracked 27 wild female Reeves’ s Pheasants from 2020 to 2023 in Hubei Province,China.We explored their clutch size and incubation behavior,as well as their responses to ambient temperature and precipitation.Clutch size averaged 7.75 ±1.36,had an association with average ambient temperature and average daily precipitation during the egglaying period,and was potentially linked to female breeding attempts.Throughout the incubation period,females took an average of 0.73 ±0.46 recesses every 24 h,with an average recess duration of 100.80 ±73.37 min and an average nest attendance of 92.98 ±5.27%.They showed a unimodal recess pattern in which nest departures peaked primarily between 13:00 and 16:00.Furthermore,females rarely left nests when daily precipitation was high.Recess duration and nest attendance were influenced by the interaction between daily mean ambient temperature and daily precipitation,as well as day of incubation.Additionally,there was a positive correlation between clutch size and recess duration.These results contribute valuable insights into the lifehistory features of this endangered species.展开更多
●AIM:To investigate the underlying mechanism of dry environment(autumn dryness)affecting the lacrimal glands in rats.●METHODS:Twenty Sprague-Dawley rats were randomly divided into two groups.The rats were fed in spe...●AIM:To investigate the underlying mechanism of dry environment(autumn dryness)affecting the lacrimal glands in rats.●METHODS:Twenty Sprague-Dawley rats were randomly divided into two groups.The rats were fed in specific pathogen free environment as the control group(n=10),and the rats fed in dry environment as the dryness group(n=10).After 24d,lacrimal glands were collected from the rats.The tissues morphology was observed by hematoxylineosin(HE)staining.Tandem mass tags(TMT)quantitative proteomics analysis technology was used to screen the differential expressed proteins of lacrimal glands between the two groups,then bioinformatics analysis was performed.Further,the immunohistochemical(IHC)method was used to verify the target proteins.●RESULTS:In dryness group,the lacrimal glands lobule atrophied,the glandular cavities enlarged,the sparse nuclear distribution and scattered inflammatory infiltration between the acinus were observed.The proteomics exhibited that a total of 195 up-regulated and 236 downregulated differential expressed proteins screened from the lacrimal glands of rats.It was indicated that the biological processes(BP)of differential expressed proteins mainly included cell processes and single BP.The cellular compositions of differential expressed proteins mainly located in cells,organelles.The molecular functions of differential expressed proteins mainly included binding,catalytic activity.Moreover,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis showed that the differential expressed proteins mainly involved lysosome,complement and coagulation cascade,and ribosome pathway.The IHC result verified that the up-regulated expression proteins of Protein S100A9(S100A9),Annexin A1(Anxa1),and Clusterin(Clu)in lacrimal glands of rats in dryness group were higher than control group.●CONCLUSION:The up-regulated expression proteins of S100A9,Anxa1,and Clu may be the potential mechanisms of dry eye symptoms caused by dry environment.This study provides clues of dry environments causing eye-related diseases for further studies.展开更多
Stress studies frequently utilize physical stressors to establish animal models of stress. In the majority of cases, these models are not consistent with human circumstances. The present study simulated a social isola...Stress studies frequently utilize physical stressors to establish animal models of stress. In the majority of cases, these models are not consistent with human circumstances. The present study simulated a social isolation plus resident intruder stress model in the rat. The rats were subjected to daily social isolation and resident intruder stress for 2 weeks. Behaviors were then tested. Rats subjected to social stress exhibited different aggressive behavior styles; some rats had greater scores on composite aggression behaviors, as well as locomotor and exploratory activity, but lower scores on latency than others. The high-aggressive group exhibited predominantly anger-out, while the low-aggressive group exhibited anger-in. Results suggest that social isolation plus resident intruder may serve as an appropriate model for anger-in and anger-out emotion modeling in the rat.展开更多
Aim: To investigate the influence of an extract obtained from five Chinese medicinal plants on sexual behavior of adult male rats. Methods: The extract was administered at doses of 30, 60 and 120 mg/kg by oral gavag...Aim: To investigate the influence of an extract obtained from five Chinese medicinal plants on sexual behavior of adult male rats. Methods: The extract was administered at doses of 30, 60 and 120 mg/kg by oral gavage, acutely (one time, 45 min before mating test) or subchronically (daily for 10 days) in sexually potent and sexually sluggish/impotent rats. Sexual behavior, serum levels of luteinizing hormone (LH) and testosterone (T) were evaluated in treated rats and compared with controls receiving vehicle. The effect of the extract on central dopaminergic neurotransmission was assessed in the nucleus accumbens using a microdialysis technique. Results: In sexually potent rats, both acute and subchronic treatment with the extract dosed at 30 and 60 mg/kg reduced mount latency and intromission latency. In sluggish/impotent rats, the acutely administered extract at the dose of 60 mg/kg shortened ejaculation latency, whereas subchronically administered at the doses of 30 and 60 mg/kg, reduced mount, intromission and ejaculation latencies, increasing also the percentage of mounting and ejaculating rats. The extract dosed at 60 mg/kg significantly increased LH and T following acute and subchronic administration and increased 3,4-dihydroxyphenylacetic acid levels in the nucleus accumbens, 30 min after the acute administration. Conclusion: The improvement in both appetitive and consummatory components of sexual behavior observed in male rats treated with the extract could be ascribed to increased serum T level in parallel with the activation of the central dopaminergic system.展开更多
BACKGROUND Wild rats have the potential to hold zoonotic infectious agents that can spread to humans and cause disease.AIM To better understand the composition of gut bacterial communities in rats is essential for pre...BACKGROUND Wild rats have the potential to hold zoonotic infectious agents that can spread to humans and cause disease.AIM To better understand the composition of gut bacterial communities in rats is essential for preventing and treating such diseases.As a tropical island located in the south of China,Hainan province has abundant rat species.Here,we examined the gut bacterial composition in wild adult rats from Hainan province.METHODS Fresh fecal samples were collected from 162 wild adult rats,including three species(Rattus norvegicus,Leopoldamys edwardsi,and Rattus losea),from nine regions of Hainan province between 2017-2018.RESULTS We analyzed the composition of gut microbiota using the 16S rRNA gene amplicon sequencing.We identified 4903 bacterial operational taxonomic units(30 phyla,175 families,and 498 genera),which vary between samples of different rat species in various habitats at various times of the year.In general,Firmicutes were the most abundant phyla,followed by Bacteroidetes(15.55%),Proteobacteria(6.13%),and Actinobacteria(4.02%).The genus Lactobacillus(20.08%),unidentified_Clostridiales(5.16%),Romboutsia(4.33%),unidentified_Ruminococcaceae(3.83%),Bacteroides(3.66%),Helicobacter(2.40%)and Streptococcus(2.37%)were dominant.CONCLUSION The composition and abundance of the gut microbial communities varied between rat species and locations.This work provides fundamental information to identify microbial communities useful for disease control in Hainan province.展开更多
The pointwise space-time behaviors of the Green’s function and the global solution to the Vlasov-Poisson-Fokker-Planck(VPFP)system in three dimensional space are studied in this paper.It is shown that the Green’s fu...The pointwise space-time behaviors of the Green’s function and the global solution to the Vlasov-Poisson-Fokker-Planck(VPFP)system in three dimensional space are studied in this paper.It is shown that the Green’s function consists of the diffusion waves decaying exponentially in time but algebraically in space,and the singular kinetic waves which become smooth for all(t,x,v)when t>0.Furthermore,we establish the pointwise space-time behaviors of the global solution to the nonlinear VPFP system when the initial data is not necessarily smooth in terms of the Green’s function.展开更多
Sanjiao acupuncture and HuangDiSan can promote the proliferation, migration and differentiation of exogenous neural stem cells in senescence-accelerated mouse prone 8 (SAMP8) mice and can improve learning and memory...Sanjiao acupuncture and HuangDiSan can promote the proliferation, migration and differentiation of exogenous neural stem cells in senescence-accelerated mouse prone 8 (SAMP8) mice and can improve learning and memory impairment and behavioral function in dementia-model mice. Thus, we sought to determine whether Sanjiao acupuncture and HuangDiSan can elevate the effect of neural stem cell transplantation in Alzheimer’s disease model mice. Sanjiao acupuncture was used to stimulate Danzhong (CV17), Zhongwan (CV12),Qihai (CV6), bilateral Xuehai (SP10) and bilateral Zusanli (ST36) 15 days before and after implantation of neural stem cells (5 × 10^5) into the hippocampal dentate gyrus of SAMP8 mice. Simultaneously, 0.2 mL HuangDiSan, containing Rehmannia Root and Chinese Angelica,was intragastrically administered. Our results demonstrated that compared with mice undergoing neural stem cell transplantation alone,learning ability was significantly improved and synaptophysin mRNA and protein levels were greatly increased in the hippocampus of mice undergoing both Sanjiao acupuncture and intragastric administration of HuangDiSan. We conclude that the combination of Sanjiao acupuncture and HuangDiSan can effectively improve dementia symptoms in mice, and the mechanism of this action might be related to the regulation of synaptophysin expression.展开更多
In this study, we investigated the effect of chronic exposure of low doses of Aluminum on affective and cognitive disorders in male and female rats. Twenty-five rats for each gender are used and the treatment carried ...In this study, we investigated the effect of chronic exposure of low doses of Aluminum on affective and cognitive disorders in male and female rats. Twenty-five rats for each gender are used and the treatment carried out for 8 weeks. Animals received distilled water for control or an intraperitoneal injection of different doses of Aluminum: 0.125, 0.25, 0.5 and 1 mg/kg. Behavioral performance is measured in various tests mainly the Open Field, Elevated Plus Maze, Force Swimming Test, Morris Water Maze, Y-maze and Object Recognition Test. Al exerts anxiogenic properties and depressive effect. The effect begins at 0.25 mg/kg to reach a maximum at 1 mg/kg. In addition, chronic exposure to Aluminum causes cognitive disorders characterized by affection of memory and influence spatial learning performance. The effect of Aluminum on working memory is effective just at 1 mg/kg, while the effect on spatial learning performance begins at 0.25 mg/kg to reach a maximum at 1 mg/kg. In conclusion, Aluminum enhances anxiety and depression parameters and cognitive disorders characterized by the affection of memory and spatial learning performance.展开更多
This study aims to evaluate the erosion behavior and the hardness of hybrid composites made of varying amounts of coconut shells,walnut shells,and carbonfibers dispersed in a polyester matrix.MINITAB(L16)Taguchi experi...This study aims to evaluate the erosion behavior and the hardness of hybrid composites made of varying amounts of coconut shells,walnut shells,and carbonfibers dispersed in a polyester matrix.MINITAB(L16)Taguchi experiments were used to determine the optimal combination of parameters.In particular,an erosion device con-sisting of a motor with a constantflow rate of 45 L/min,a pump with a diameter of 40 mm,a nozzle with a dia-meter of 5 mm,and a tank made of“perspex glass”55 cm long,30 cm tall,and 25 cm wide was used.The tests were conducted by varying the sample-to-nozzle distance,the pattern angle,and the sand particle size.The results have revealed that the presence of 7.5%by weight of waste coconut shell,for conditions corresponding to 90°angle,sand size 425μm,stand distance 30 cm,gives the best wear resistance(3.04×10^(-5) g/g).Thefiller content and sand particle size affect the erosive rate,with the angle playing a secondary role.The distance between the sample and the nozzle has a weaker effect on erosive wear.The hardness results show that the models(UP-5%carbonfiber-2.5-3.5-4.5-5.5-6.5-7.5 wt.%waste coconut shell)give the best values for prayer compared to the samples(UP-5 wt.%carbonfiber-2.5-3.5-4.5-5.5-6.5-7.5 wt.%waste walnut shell).展开更多
Wistar rats were exposed to a 916 MHz, 10 W/m2 mobile phone electromagnetic field for 6 hours a day 5 days a week. Average completion times in an eight-arm radial maze were longer in the exposed rats than control rats...Wistar rats were exposed to a 916 MHz, 10 W/m2 mobile phone electromagnetic field for 6 hours a day 5 days a week. Average completion times in an eight-arm radial maze were longer in the exposed rats than control rats after 4-5 weeks of exposure. Error rates in the exposed rats were greater than the control rats at 6 weeks. Hippocampal neurons from the exposed rats showed irregular firing patterns during the experiment, and they exhibited decreased spiking activity 6-9 weeks compared with that after 2 5 weeks of exposure. These results indicate that 916 MHz electromagnetic fields influence leaming and memory in rats during exposure, but long-term effects are not obvious.展开更多
The effect of complete social deprivation in early postnatal ontogenesis on adult behavior in Wag/Rij rats with a genetic predisposition to the development of absence epilepsy was studied. From the 2nd to the 8th day ...The effect of complete social deprivation in early postnatal ontogenesis on adult behavior in Wag/Rij rats with a genetic predisposition to the development of absence epilepsy was studied. From the 2nd to the 8th day of life, a 3-hour daily isolation of the pups from the mother and siblings was performed, the control animals were handled. In adulthood, offspring behavior was tested in an elevated plus-maze, an open field, and for an ability to learn the conditioned reflex of active avoidance. In isolated earlier rats, an increase in emotional reactivity, exploratory behavior in a potentially dangerous space, the speed of learning a conditioned reflex with negative painful reinforcement, and a disturbance of the formation of a defensive reaction were revealed. It is assumed that high emotional reactivity creates the prerequisites for the development of absence epilepsy.展开更多
Ethanol affects sexual behavior drastically, as much as its execution as well as its motivation. Ethanol is a central nervous system depressant and the prefrontal cortex (PFC) is one of the cortical areas most sensiti...Ethanol affects sexual behavior drastically, as much as its execution as well as its motivation. Ethanol is a central nervous system depressant and the prefrontal cortex (PFC) is one of the cortical areas most sensitive to its effects. This prefrontal area participates in the modulation of the cognitive control, and furthermore, by mean of connections with the parietal cortex (PC) plays an important role in the processing of sexually relevant stimuli. Considering that it has been reported that the ethanol affects the proper functioning of these cortical areas, particularly decreasing the degree of electroencephalographic (EEG) coupling or correlation between them, the aim of this study was to characterize the effect of a low dose of ethanol on EEG correlation, as well determining if this effect is associated with impaired sexual behavior in male rats. Nine male rats unilaterally implanted were simultaneously recorded the EEG from the PFC and PC to calculate the degree of EEG synchronization (correlation, r) between both cortices during the first 40 minutes post administration of 0.75 g/kg of ethanol. At the end of the EEG recording, the males were submitted to copulatory behavior with a receptive female rat. The ethanol administration was associated with a decreased intrahemispheric correlation of the fast bands between PFC and PC, as well as with an increased mount and intromission latency. This data showed that ethanol in moderate doses decreased the coupling degree between cortices. It is likely that this functional disconnection between the PFC and PC after the ethanol administration could be associated with the difficulty in discriminating incentive value of the sexual stimuli so that decreased the sexual motivation but not the performance of the copulatory parameters in the male rat.展开更多
Argan oil is renowned for its particular biochemical profile: high-fat oleic and linoleic acids, tocopherols, sterols, polyphenols. This composition gives it nutritional, therapeutic and preventive properties against ...Argan oil is renowned for its particular biochemical profile: high-fat oleic and linoleic acids, tocopherols, sterols, polyphenols. This composition gives it nutritional, therapeutic and preventive properties against dermatological, metabolic and proliferative diseases. The composition of argan oil assigns its benefits to mental health;it would be provided with possible effects on the prevention and/or cure of stress related disorder. This work aims to evaluate the impact of argan oil dietary on the behavioral response, biochemical and hematological constants and histological profiles of adrenal involved in emotional responses to stress. The variation of these parameters was evaluated in Wistar rats receiving dietary 10 ml/Kg/day of argan oil, starting from weaning, for 13 weeks. Our results show that supplementation has resulted in an increase in locomotor activity, reduced sensitivity to frightening environments with sex dependent variation. Moreover, lipid markers, corticosterone and lymphocytes show a rising trend. If the important role of argan oil diet in cardio-metabolic health is generally well recognized;for mental health, it is the first study that needs further investigation linking between the nervous system, inflammation parameters and metabolism.展开更多
Quantitative trait loci (QTLs) mapping has been performed during the past decades in an attempt to identify genes, gene products and mechanisms underlying numerous quantitative traits. It’s a strategy based on natura...Quantitative trait loci (QTLs) mapping has been performed during the past decades in an attempt to identify genes, gene products and mechanisms underlying numerous quantitative traits. It’s a strategy based on natural variations in genes and gene products, which facilitates translation from animal models to human clinical conditions. Our team has shown that the inbred rat strains Lewis (LEW) and Spontaneously Hypertensive Rats (SHR) differ with respect to several emotionality- related behaviors, one of which (inner locomotion in the open field) was strongly influenced by a QTL (Anxrr16) on rat chromosome 4. Since then, several other studies not only corroborated the initial description of Anxrr16, but also extrapolated its effects to a broader context (rats from both sexes and regardless of the estrous cycle phase) and suggested that this same region influences other emotionality-related behaviors as well as alcohol intake. Other QTLs affecting neurobiological traits were also found on rat chromosome 4 and several candidate genes have been pointed out as possibly influencing those phenotypes. Altogether, these studies suggest that rat chromosome 4 constitutes an interesting target for the study of the molecular bases of anxiety and other traits related to emotional reactivity.展开更多
The Roman high(RHA) and low-Avoidance (RLA) rats were selectively bred for rapid vs poor acquisition of two-way active avoidance behavior. These lines differ in numerous behavioral traits, with RLA rats being more fea...The Roman high(RHA) and low-Avoidance (RLA) rats were selectively bred for rapid vs poor acquisition of two-way active avoidance behavior. These lines differ in numerous behavioral traits, with RLA rats being more fearful/anxious than RHA rats, and the latter being novelty-seekers and showing larger intake of, and preference for, addictive substances including ethanol (ETH). Moreover, several differences in central dopaminergic, serotonergic, and GABAergic functions have been reported in these two lines. Since those neural systems are involved in the regulation of ETH consumption, it was considered of interest to investigate: 1) the differences in ETH intake and preference between RHA and RLA rats, 2) the effects of ETH on DA release in the shell of the nucleus accumbens (AcbSh) using brain microdialysis. ETH solutions of increasing concentrations (2% - 10%) were presented on alternate days in a free choice with water. To examine ETH intake and preference stability, animals were subsequently switched to daily presentations of 10% ETH for 10 consecutive days. RHA rats consumed significantly larger amounts of ETH and displayed higher ETH preference than did RLA rats throughout the acquisition and maintenance phases. Following chronic exposure to ETH the animals were habituated to a restricted access to ETH schedule (2% ETH, 2 h per day × 4 days) before surgical implantation of a dialysis probe in the AcbSh. Under these experimental conditions, voluntary ETH intake (2%, 1 h, p.o.) produced a significant increase in accumbal DA output in RHA rats but not in their RLA counterparts. Finally, the i.p. administration of ETH (0.25 g/kg) to na?ve Roman rats produced a significant increment in accumbal DA output only in RHA rats. These results indicate that the mesolimbic dopaminergic system of RHA rats is more responsive to the effects of ETH than that of RLA rats.展开更多
Male Sprague-Dawley rats administered xylene intrapetitoneally on alternate days at a dose of 125 or 250mg/kg for 30 days exhibited no marked changes in locomotor activity, learning and memory capacity. However in rat...Male Sprague-Dawley rats administered xylene intrapetitoneally on alternate days at a dose of 125 or 250mg/kg for 30 days exhibited no marked changes in locomotor activity, learning and memory capacity. However in rats given xylene on alternate day at a dose of 500 mg/kg for 30 days, a significant decrease in locomotor activity, deficits in leaming ability and memory loss were detected. These xylene-induced behavioral ehanges were assoryiated with a decrease in fyendorphin and leuenkaphlin concentrations in the pons-medulla. On the contrary, xylene at a dose of 500mg/kg increased the β-endorphin level in caudate and c-fos expression in hippocampus. These data suggest that the xylene-induced behavioral alterations might be associated with the expression of Fos protein in the hippocampus.展开更多
基金supported by the National Natural Science Foundation of China (grant number 31872240)。
文摘Weather conditions play a pivotal role in embryo development and parental incubation costs,potentially impacting the clutch size and incubation behavior of birds.Understanding these effects is crucial for bird conservation.Reeves’ s Pheasant(Syrmaticus reevesii) is a threatened species endemic to China,which is characterized by female-only incubation.However,there is a lack of information regarding the impact of weather conditions on clutch size and incubation behavior in this species.Using satellite tracking,we tracked 27 wild female Reeves’ s Pheasants from 2020 to 2023 in Hubei Province,China.We explored their clutch size and incubation behavior,as well as their responses to ambient temperature and precipitation.Clutch size averaged 7.75 ±1.36,had an association with average ambient temperature and average daily precipitation during the egglaying period,and was potentially linked to female breeding attempts.Throughout the incubation period,females took an average of 0.73 ±0.46 recesses every 24 h,with an average recess duration of 100.80 ±73.37 min and an average nest attendance of 92.98 ±5.27%.They showed a unimodal recess pattern in which nest departures peaked primarily between 13:00 and 16:00.Furthermore,females rarely left nests when daily precipitation was high.Recess duration and nest attendance were influenced by the interaction between daily mean ambient temperature and daily precipitation,as well as day of incubation.Additionally,there was a positive correlation between clutch size and recess duration.These results contribute valuable insights into the lifehistory features of this endangered species.
基金Supported by Regional Science Foundation Project of the National Natural Science Foundation of China(No.82060827,No.82260891)The Key Discipline of Universities in the“14th Five-Year Plan”Autonomous Region-Traditional Chinese Medicine at Xinjiang Medical University.
文摘●AIM:To investigate the underlying mechanism of dry environment(autumn dryness)affecting the lacrimal glands in rats.●METHODS:Twenty Sprague-Dawley rats were randomly divided into two groups.The rats were fed in specific pathogen free environment as the control group(n=10),and the rats fed in dry environment as the dryness group(n=10).After 24d,lacrimal glands were collected from the rats.The tissues morphology was observed by hematoxylineosin(HE)staining.Tandem mass tags(TMT)quantitative proteomics analysis technology was used to screen the differential expressed proteins of lacrimal glands between the two groups,then bioinformatics analysis was performed.Further,the immunohistochemical(IHC)method was used to verify the target proteins.●RESULTS:In dryness group,the lacrimal glands lobule atrophied,the glandular cavities enlarged,the sparse nuclear distribution and scattered inflammatory infiltration between the acinus were observed.The proteomics exhibited that a total of 195 up-regulated and 236 downregulated differential expressed proteins screened from the lacrimal glands of rats.It was indicated that the biological processes(BP)of differential expressed proteins mainly included cell processes and single BP.The cellular compositions of differential expressed proteins mainly located in cells,organelles.The molecular functions of differential expressed proteins mainly included binding,catalytic activity.Moreover,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis showed that the differential expressed proteins mainly involved lysosome,complement and coagulation cascade,and ribosome pathway.The IHC result verified that the up-regulated expression proteins of Protein S100A9(S100A9),Annexin A1(Anxa1),and Clusterin(Clu)in lacrimal glands of rats in dryness group were higher than control group.●CONCLUSION:The up-regulated expression proteins of S100A9,Anxa1,and Clu may be the potential mechanisms of dry eye symptoms caused by dry environment.This study provides clues of dry environments causing eye-related diseases for further studies.
基金the National Natural Science Foundation of China,No.30973688the National Program of Key Basic Research Project(973Program),No.2006CB504804
文摘Stress studies frequently utilize physical stressors to establish animal models of stress. In the majority of cases, these models are not consistent with human circumstances. The present study simulated a social isolation plus resident intruder stress model in the rat. The rats were subjected to daily social isolation and resident intruder stress for 2 weeks. Behaviors were then tested. Rats subjected to social stress exhibited different aggressive behavior styles; some rats had greater scores on composite aggression behaviors, as well as locomotor and exploratory activity, but lower scores on latency than others. The high-aggressive group exhibited predominantly anger-out, while the low-aggressive group exhibited anger-in. Results suggest that social isolation plus resident intruder may serve as an appropriate model for anger-in and anger-out emotion modeling in the rat.
文摘Aim: To investigate the influence of an extract obtained from five Chinese medicinal plants on sexual behavior of adult male rats. Methods: The extract was administered at doses of 30, 60 and 120 mg/kg by oral gavage, acutely (one time, 45 min before mating test) or subchronically (daily for 10 days) in sexually potent and sexually sluggish/impotent rats. Sexual behavior, serum levels of luteinizing hormone (LH) and testosterone (T) were evaluated in treated rats and compared with controls receiving vehicle. The effect of the extract on central dopaminergic neurotransmission was assessed in the nucleus accumbens using a microdialysis technique. Results: In sexually potent rats, both acute and subchronic treatment with the extract dosed at 30 and 60 mg/kg reduced mount latency and intromission latency. In sluggish/impotent rats, the acutely administered extract at the dose of 60 mg/kg shortened ejaculation latency, whereas subchronically administered at the doses of 30 and 60 mg/kg, reduced mount, intromission and ejaculation latencies, increasing also the percentage of mounting and ejaculating rats. The extract dosed at 60 mg/kg significantly increased LH and T following acute and subchronic administration and increased 3,4-dihydroxyphenylacetic acid levels in the nucleus accumbens, 30 min after the acute administration. Conclusion: The improvement in both appetitive and consummatory components of sexual behavior observed in male rats treated with the extract could be ascribed to increased serum T level in parallel with the activation of the central dopaminergic system.
基金Supported by Hainan Province Science and Technology Special Fund,No.ZDYF2022SHFZ114Hainan Provincial Natural Science Foundation of China,No.820RC650+1 种基金National Natural Science Foundation of China,No.82060377Innovative Research Project for Graduate Students of Hainan Medical University,No.HYYS2020-18,No.HYYS2021A09,and No.HYYS2021A22.
文摘BACKGROUND Wild rats have the potential to hold zoonotic infectious agents that can spread to humans and cause disease.AIM To better understand the composition of gut bacterial communities in rats is essential for preventing and treating such diseases.As a tropical island located in the south of China,Hainan province has abundant rat species.Here,we examined the gut bacterial composition in wild adult rats from Hainan province.METHODS Fresh fecal samples were collected from 162 wild adult rats,including three species(Rattus norvegicus,Leopoldamys edwardsi,and Rattus losea),from nine regions of Hainan province between 2017-2018.RESULTS We analyzed the composition of gut microbiota using the 16S rRNA gene amplicon sequencing.We identified 4903 bacterial operational taxonomic units(30 phyla,175 families,and 498 genera),which vary between samples of different rat species in various habitats at various times of the year.In general,Firmicutes were the most abundant phyla,followed by Bacteroidetes(15.55%),Proteobacteria(6.13%),and Actinobacteria(4.02%).The genus Lactobacillus(20.08%),unidentified_Clostridiales(5.16%),Romboutsia(4.33%),unidentified_Ruminococcaceae(3.83%),Bacteroides(3.66%),Helicobacter(2.40%)and Streptococcus(2.37%)were dominant.CONCLUSION The composition and abundance of the gut microbial communities varied between rat species and locations.This work provides fundamental information to identify microbial communities useful for disease control in Hainan province.
基金supported by National Natural Science Foundation of China(11671100 and 12171104)the National Science Fund for Excellent Young Scholars(11922107)Guangxi Natural Science Foundation(2018GXNSFAA138210 and 2019JJG110010)。
文摘The pointwise space-time behaviors of the Green’s function and the global solution to the Vlasov-Poisson-Fokker-Planck(VPFP)system in three dimensional space are studied in this paper.It is shown that the Green’s function consists of the diffusion waves decaying exponentially in time but algebraically in space,and the singular kinetic waves which become smooth for all(t,x,v)when t>0.Furthermore,we establish the pointwise space-time behaviors of the global solution to the nonlinear VPFP system when the initial data is not necessarily smooth in terms of the Green’s function.
基金supported by the National Natural Science Foundation of China,No.81202740 and 81603686the Natural Science Foundation of Tianjin of China,No.17JCYBJC26200 and 12JCQNJC07400+1 种基金the Public Health Bureau Science and Technology Foundation of Tianjin of China,No.2014KY15the Specialized Research Foundation for the Doctoral Program of Higher Education,No.20121210120002
文摘Sanjiao acupuncture and HuangDiSan can promote the proliferation, migration and differentiation of exogenous neural stem cells in senescence-accelerated mouse prone 8 (SAMP8) mice and can improve learning and memory impairment and behavioral function in dementia-model mice. Thus, we sought to determine whether Sanjiao acupuncture and HuangDiSan can elevate the effect of neural stem cell transplantation in Alzheimer’s disease model mice. Sanjiao acupuncture was used to stimulate Danzhong (CV17), Zhongwan (CV12),Qihai (CV6), bilateral Xuehai (SP10) and bilateral Zusanli (ST36) 15 days before and after implantation of neural stem cells (5 × 10^5) into the hippocampal dentate gyrus of SAMP8 mice. Simultaneously, 0.2 mL HuangDiSan, containing Rehmannia Root and Chinese Angelica,was intragastrically administered. Our results demonstrated that compared with mice undergoing neural stem cell transplantation alone,learning ability was significantly improved and synaptophysin mRNA and protein levels were greatly increased in the hippocampus of mice undergoing both Sanjiao acupuncture and intragastric administration of HuangDiSan. We conclude that the combination of Sanjiao acupuncture and HuangDiSan can effectively improve dementia symptoms in mice, and the mechanism of this action might be related to the regulation of synaptophysin expression.
文摘In this study, we investigated the effect of chronic exposure of low doses of Aluminum on affective and cognitive disorders in male and female rats. Twenty-five rats for each gender are used and the treatment carried out for 8 weeks. Animals received distilled water for control or an intraperitoneal injection of different doses of Aluminum: 0.125, 0.25, 0.5 and 1 mg/kg. Behavioral performance is measured in various tests mainly the Open Field, Elevated Plus Maze, Force Swimming Test, Morris Water Maze, Y-maze and Object Recognition Test. Al exerts anxiogenic properties and depressive effect. The effect begins at 0.25 mg/kg to reach a maximum at 1 mg/kg. In addition, chronic exposure to Aluminum causes cognitive disorders characterized by affection of memory and influence spatial learning performance. The effect of Aluminum on working memory is effective just at 1 mg/kg, while the effect on spatial learning performance begins at 0.25 mg/kg to reach a maximum at 1 mg/kg. In conclusion, Aluminum enhances anxiety and depression parameters and cognitive disorders characterized by the affection of memory and spatial learning performance.
基金Supported by Natural Science Foundation of Jiangsu Province,NO.BK2005033Medical Foundation of Department of Health,Jiangsu Province, No. H200325+1 种基金Natural Science Foundation of Department of Education, Jiangsu Province, No. 04kJB320127Medical Foundation of Department of Health, Zhejiang Province, No. 2004A084
文摘瞄准:证实内脏的疼痛经由颜色为表面的扩张(CRD ) 建模并且由在老鼠测量腹的屈肌反射(AWR ) 的分数评估 CRD 的行为的回答的效率。方法:称 180-240 g 的 38 只男 SD 老鼠被用来建立内脏的疼痛模型。老鼠与 7 厘米 intra-anally 被插入在醚麻醉下面的长灵活的乳胶汽球,和由与空气使汽球膨胀的表面的扩张在从麻醉恢复以后被成为 30 min 的颜色。五个 AWR 分数(到 AWR4 的 AWR0 ) 被用来估计有害内脏的刺激的紧张。它被认为是最低压(kPa ) 的阀值。因为腹的 flatting 被颜色导致表面的扩张。结果:到下降冒号和直肠的扩张的精力旺盛的 AWR 在 100% 被发现醒着测试的老鼠。越高扩张的压力,越 higher AWR 的分数。0, 2.00, 3.33, 5.33 和 8.00 kPa 的扩张压力生产了不同 AWR 分数(P【0.05 ) 。AWR 的痛觉阈为在起始的终结(第一 1-3 扩张) 以后的多达 80 min 是不变的,吝啬的阀值是 3.69+/-0.35 kPa。吗啡硫酸盐的全身的管理在一个剂量依赖者和 naloxone 提高了内脏的疼痛的阀值可逆举止。结论:在表面的扩张能估计的颜色期间获得 AWR 有害内脏的刺激的紧张。到内脏的痛觉阈清楚、不变、可靠的 CRD 的腹部(AWR 3 ) 的 Flatting。这个疼痛模型和它的行为的评价对关于内脏的疼痛和止痛剂的研究好。
文摘This study aims to evaluate the erosion behavior and the hardness of hybrid composites made of varying amounts of coconut shells,walnut shells,and carbonfibers dispersed in a polyester matrix.MINITAB(L16)Taguchi experiments were used to determine the optimal combination of parameters.In particular,an erosion device con-sisting of a motor with a constantflow rate of 45 L/min,a pump with a diameter of 40 mm,a nozzle with a dia-meter of 5 mm,and a tank made of“perspex glass”55 cm long,30 cm tall,and 25 cm wide was used.The tests were conducted by varying the sample-to-nozzle distance,the pattern angle,and the sand particle size.The results have revealed that the presence of 7.5%by weight of waste coconut shell,for conditions corresponding to 90°angle,sand size 425μm,stand distance 30 cm,gives the best wear resistance(3.04×10^(-5) g/g).Thefiller content and sand particle size affect the erosive rate,with the angle playing a secondary role.The distance between the sample and the nozzle has a weaker effect on erosive wear.The hardness results show that the models(UP-5%carbonfiber-2.5-3.5-4.5-5.5-6.5-7.5 wt.%waste coconut shell)give the best values for prayer compared to the samples(UP-5 wt.%carbonfiber-2.5-3.5-4.5-5.5-6.5-7.5 wt.%waste walnut shell).
基金funded by the National Basic Research Program of China(973 Program),No. 2011CB503700,2011CB503702the National Natural Science Foundation of China,No.81071231,30670543the Research Program of Beijing Municipal Education Commission,No. KM200910005016,and No.JX015999201001
文摘Wistar rats were exposed to a 916 MHz, 10 W/m2 mobile phone electromagnetic field for 6 hours a day 5 days a week. Average completion times in an eight-arm radial maze were longer in the exposed rats than control rats after 4-5 weeks of exposure. Error rates in the exposed rats were greater than the control rats at 6 weeks. Hippocampal neurons from the exposed rats showed irregular firing patterns during the experiment, and they exhibited decreased spiking activity 6-9 weeks compared with that after 2 5 weeks of exposure. These results indicate that 916 MHz electromagnetic fields influence leaming and memory in rats during exposure, but long-term effects are not obvious.
文摘The effect of complete social deprivation in early postnatal ontogenesis on adult behavior in Wag/Rij rats with a genetic predisposition to the development of absence epilepsy was studied. From the 2nd to the 8th day of life, a 3-hour daily isolation of the pups from the mother and siblings was performed, the control animals were handled. In adulthood, offspring behavior was tested in an elevated plus-maze, an open field, and for an ability to learn the conditioned reflex of active avoidance. In isolated earlier rats, an increase in emotional reactivity, exploratory behavior in a potentially dangerous space, the speed of learning a conditioned reflex with negative painful reinforcement, and a disturbance of the formation of a defensive reaction were revealed. It is assumed that high emotional reactivity creates the prerequisites for the development of absence epilepsy.
文摘Ethanol affects sexual behavior drastically, as much as its execution as well as its motivation. Ethanol is a central nervous system depressant and the prefrontal cortex (PFC) is one of the cortical areas most sensitive to its effects. This prefrontal area participates in the modulation of the cognitive control, and furthermore, by mean of connections with the parietal cortex (PC) plays an important role in the processing of sexually relevant stimuli. Considering that it has been reported that the ethanol affects the proper functioning of these cortical areas, particularly decreasing the degree of electroencephalographic (EEG) coupling or correlation between them, the aim of this study was to characterize the effect of a low dose of ethanol on EEG correlation, as well determining if this effect is associated with impaired sexual behavior in male rats. Nine male rats unilaterally implanted were simultaneously recorded the EEG from the PFC and PC to calculate the degree of EEG synchronization (correlation, r) between both cortices during the first 40 minutes post administration of 0.75 g/kg of ethanol. At the end of the EEG recording, the males were submitted to copulatory behavior with a receptive female rat. The ethanol administration was associated with a decreased intrahemispheric correlation of the fast bands between PFC and PC, as well as with an increased mount and intromission latency. This data showed that ethanol in moderate doses decreased the coupling degree between cortices. It is likely that this functional disconnection between the PFC and PC after the ethanol administration could be associated with the difficulty in discriminating incentive value of the sexual stimuli so that decreased the sexual motivation but not the performance of the copulatory parameters in the male rat.
文摘Argan oil is renowned for its particular biochemical profile: high-fat oleic and linoleic acids, tocopherols, sterols, polyphenols. This composition gives it nutritional, therapeutic and preventive properties against dermatological, metabolic and proliferative diseases. The composition of argan oil assigns its benefits to mental health;it would be provided with possible effects on the prevention and/or cure of stress related disorder. This work aims to evaluate the impact of argan oil dietary on the behavioral response, biochemical and hematological constants and histological profiles of adrenal involved in emotional responses to stress. The variation of these parameters was evaluated in Wistar rats receiving dietary 10 ml/Kg/day of argan oil, starting from weaning, for 13 weeks. Our results show that supplementation has resulted in an increase in locomotor activity, reduced sensitivity to frightening environments with sex dependent variation. Moreover, lipid markers, corticosterone and lymphocytes show a rising trend. If the important role of argan oil diet in cardio-metabolic health is generally well recognized;for mental health, it is the first study that needs further investigation linking between the nervous system, inflammation parameters and metabolism.
文摘Quantitative trait loci (QTLs) mapping has been performed during the past decades in an attempt to identify genes, gene products and mechanisms underlying numerous quantitative traits. It’s a strategy based on natural variations in genes and gene products, which facilitates translation from animal models to human clinical conditions. Our team has shown that the inbred rat strains Lewis (LEW) and Spontaneously Hypertensive Rats (SHR) differ with respect to several emotionality- related behaviors, one of which (inner locomotion in the open field) was strongly influenced by a QTL (Anxrr16) on rat chromosome 4. Since then, several other studies not only corroborated the initial description of Anxrr16, but also extrapolated its effects to a broader context (rats from both sexes and regardless of the estrous cycle phase) and suggested that this same region influences other emotionality-related behaviors as well as alcohol intake. Other QTLs affecting neurobiological traits were also found on rat chromosome 4 and several candidate genes have been pointed out as possibly influencing those phenotypes. Altogether, these studies suggest that rat chromosome 4 constitutes an interesting target for the study of the molecular bases of anxiety and other traits related to emotional reactivity.
文摘The Roman high(RHA) and low-Avoidance (RLA) rats were selectively bred for rapid vs poor acquisition of two-way active avoidance behavior. These lines differ in numerous behavioral traits, with RLA rats being more fearful/anxious than RHA rats, and the latter being novelty-seekers and showing larger intake of, and preference for, addictive substances including ethanol (ETH). Moreover, several differences in central dopaminergic, serotonergic, and GABAergic functions have been reported in these two lines. Since those neural systems are involved in the regulation of ETH consumption, it was considered of interest to investigate: 1) the differences in ETH intake and preference between RHA and RLA rats, 2) the effects of ETH on DA release in the shell of the nucleus accumbens (AcbSh) using brain microdialysis. ETH solutions of increasing concentrations (2% - 10%) were presented on alternate days in a free choice with water. To examine ETH intake and preference stability, animals were subsequently switched to daily presentations of 10% ETH for 10 consecutive days. RHA rats consumed significantly larger amounts of ETH and displayed higher ETH preference than did RLA rats throughout the acquisition and maintenance phases. Following chronic exposure to ETH the animals were habituated to a restricted access to ETH schedule (2% ETH, 2 h per day × 4 days) before surgical implantation of a dialysis probe in the AcbSh. Under these experimental conditions, voluntary ETH intake (2%, 1 h, p.o.) produced a significant increase in accumbal DA output in RHA rats but not in their RLA counterparts. Finally, the i.p. administration of ETH (0.25 g/kg) to na?ve Roman rats produced a significant increment in accumbal DA output only in RHA rats. These results indicate that the mesolimbic dopaminergic system of RHA rats is more responsive to the effects of ETH than that of RLA rats.
文摘Male Sprague-Dawley rats administered xylene intrapetitoneally on alternate days at a dose of 125 or 250mg/kg for 30 days exhibited no marked changes in locomotor activity, learning and memory capacity. However in rats given xylene on alternate day at a dose of 500 mg/kg for 30 days, a significant decrease in locomotor activity, deficits in leaming ability and memory loss were detected. These xylene-induced behavioral ehanges were assoryiated with a decrease in fyendorphin and leuenkaphlin concentrations in the pons-medulla. On the contrary, xylene at a dose of 500mg/kg increased the β-endorphin level in caudate and c-fos expression in hippocampus. These data suggest that the xylene-induced behavioral alterations might be associated with the expression of Fos protein in the hippocampus.