Patatin-like phospholipase domain containing 5 （PNPLA5） is a neotype neutral lipase with dual activity of anabolism and catabolism in vitro and in vivo, which has a low mRNA expression level in humans and mice. PNPL...Patatin-like phospholipase domain containing 5 （PNPLA5） is a neotype neutral lipase with dual activity of anabolism and catabolism in vitro and in vivo, which has a low mRNA expression level in humans and mice. PNPLA5, which is localized to lipid droplets and required for efficient autophagy by optimal initiation, has been speculated to possess triglyceride hydro- lase activity, and has been associated with low density lipoprotein cholesterol （LDL-C）. Above all, PNPLA5 is a relatively new gene, which is reported less about its biological function research, especially the function research in the rats is still blank. In this study, we examined the spatiotemporal expression profile of PNPLA5 and found that it was expressed at low levels in most organs of Sprague Dawley （SD） rats, but was present at very high levels in the skin and testes. To furth.er determine the biological function of PNPLA5 in mammals, we generated PNPLA5-knockout SD rats using the clustered regularly-interspaced short palindromic repeats （CRISPR）/Cas9 system. PNPLA5-null rats were viable, but showed a variety of phenotypic abnormalities, such as abnormal bleeding, and varied hematobiochemical parameters including increased serum total cholesterol （TC）, tdglycerides and high density lipoprotein cholesterol （HDL-C） level, and reduced LDL-C level, compared with wild-type control rats. These data are consistent with an important role for PNPLA5 in lipid metabolism, provJdJng a new target gene and animal model for treatment of cardiovascular diseases in the future.展开更多
Objective : To investigate the effect of Huan shuai Recipe on the expression level of fibronectin (FN) and transforming growth factor β 2 (TGF β 2 ) in the remnant kidney of rats. Methods : Male Wistar ra...Objective : To investigate the effect of Huan shuai Recipe on the expression level of fibronectin (FN) and transforming growth factor β 2 (TGF β 2 ) in the remnant kidney of rats. Methods : Male Wistar rats, after 4 weeks of 5/6 nephrectomy, were divided into two groups at random, and treated with water and Huanshuai Recipe (HSR) respectively. Serum creatinine (SCr) and urinary protein of 24 hours were observed at beginning, 6th week and 12th week of the experiment, and FN and TGF β 2 were assayed by immunohistochemical technique and computer assisted image analysis as well. Results : Serum SCr and urinary protein of 24 hours were increased significantly in the animals after 5/6 nephrectomy. The increased parameters decreased in the HSR group after treatment, as compared with those in the control group, and the difference was significant ( P <0.05 ); Immunohistochemical measurement and computer assisted image analysis showed a marked suppression of FN and TGF β 2 in animals of the HSR group with significant difference from those in the control group ( P <0.05). Conclusion : HSR not only plays the role of renal function protection, but could also alleviate the glomerular pathological injury. The effect on TGF β 2 and FN may be the mechanism of HSR in slowing down the progression of chronic renal failure.展开更多
基金funded by the National Natural Science Foundation of China(31572378)the Major National Scientific Research Projects,China(2015CB943101)the Agricultural Science and Technology Innovation Program,China(ASTIP-IAS05)
文摘Patatin-like phospholipase domain containing 5 （PNPLA5） is a neotype neutral lipase with dual activity of anabolism and catabolism in vitro and in vivo, which has a low mRNA expression level in humans and mice. PNPLA5, which is localized to lipid droplets and required for efficient autophagy by optimal initiation, has been speculated to possess triglyceride hydro- lase activity, and has been associated with low density lipoprotein cholesterol （LDL-C）. Above all, PNPLA5 is a relatively new gene, which is reported less about its biological function research, especially the function research in the rats is still blank. In this study, we examined the spatiotemporal expression profile of PNPLA5 and found that it was expressed at low levels in most organs of Sprague Dawley （SD） rats, but was present at very high levels in the skin and testes. To furth.er determine the biological function of PNPLA5 in mammals, we generated PNPLA5-knockout SD rats using the clustered regularly-interspaced short palindromic repeats （CRISPR）/Cas9 system. PNPLA5-null rats were viable, but showed a variety of phenotypic abnormalities, such as abnormal bleeding, and varied hematobiochemical parameters including increased serum total cholesterol （TC）, tdglycerides and high density lipoprotein cholesterol （HDL-C） level, and reduced LDL-C level, compared with wild-type control rats. These data are consistent with an important role for PNPLA5 in lipid metabolism, provJdJng a new target gene and animal model for treatment of cardiovascular diseases in the future.
文摘Objective : To investigate the effect of Huan shuai Recipe on the expression level of fibronectin (FN) and transforming growth factor β 2 (TGF β 2 ) in the remnant kidney of rats. Methods : Male Wistar rats, after 4 weeks of 5/6 nephrectomy, were divided into two groups at random, and treated with water and Huanshuai Recipe (HSR) respectively. Serum creatinine (SCr) and urinary protein of 24 hours were observed at beginning, 6th week and 12th week of the experiment, and FN and TGF β 2 were assayed by immunohistochemical technique and computer assisted image analysis as well. Results : Serum SCr and urinary protein of 24 hours were increased significantly in the animals after 5/6 nephrectomy. The increased parameters decreased in the HSR group after treatment, as compared with those in the control group, and the difference was significant ( P <0.05 ); Immunohistochemical measurement and computer assisted image analysis showed a marked suppression of FN and TGF β 2 in animals of the HSR group with significant difference from those in the control group ( P <0.05). Conclusion : HSR not only plays the role of renal function protection, but could also alleviate the glomerular pathological injury. The effect on TGF β 2 and FN may be the mechanism of HSR in slowing down the progression of chronic renal failure.
