To provide "more reasonable, more saving and more efficient" water and fertilizer application proposals, taking ‘Yujiao 5' as the experimental material, the effects of different irrigation times and nitrogen appli...To provide "more reasonable, more saving and more efficient" water and fertilizer application proposals, taking ‘Yujiao 5' as the experimental material, the effects of different irrigation times and nitrogen application treatments on the leaf area index and yield of wheat were studied using three-factor split plot method. The results showed that irrigation times, nitrogen application rate and the ratio of basa to topdressed nitrogen respectively had significant effects on the leaf area index, the yield and component factors of wheat. Under the treatment of W1(irrigation before sowing), the leaf area index showed a positive linear correlation with nitrogen application rate; under the treatments of W2(irrigation before sowing and at jointing stage) and W3(irrigation before sowing, at jointing stage and at grain filling stages),the leaf area index showed a positive linear correlation with nitrogen application rate at the jointing stage, booting stage and heading stage; 20 d after heading, the leaf area index showed a quadric curve relationship with nitrogen application rate at these stages, and the LAI of N3R2 was the highest. Under different irrigation times,the yield, ear number and kernels per ear showed quadric curve relationship with nitrogen application rate, 1 000-seed weight showed the trend of linear decrease with the increase of nitrogen application rate. Under the treatment combination of irrigation before sowing, at jointing stage and at grain filling stage, nitrogen application rate at 240 kg/hm^2 and the ratio of basal to topdressed nitrogen of 5:5, the grain yield(8 609.60 kg/hm^2), ear number(688.2×104/hm^2) and kernel number per ear(37.9 grains) reached the highest value at W3N3R2, and the grain yield of W3N3R2 increased by 144.8% compared to the W1N0. In conclusion, in Eastern Henan where the rainfall is insufficient at the late growth stage of wheat, the irrigation-saving space in wheat production is relatively small, but the nitrogen-saving space is relatively large.展开更多
Short basal internodes are important for lodging resistance of rice(Oryza sativa L.).Several canopy indices affect the elongation of basal internodes,but uncertainty as to the key factors determining elongation of bas...Short basal internodes are important for lodging resistance of rice(Oryza sativa L.).Several canopy indices affect the elongation of basal internodes,but uncertainty as to the key factors determining elongation of basal internodes persists.The objectives of this study were(1)to identify key factors affecting the elongation of basal internodes and(2)to establish a quantitative relationship between basal internode length and canopy indices.An inbred rice cultivar,Yinjingruanzhan,was grown in two split-plot field experiments with three N rates(0,75,and 150 kg N ha−1 in early season and 0,90,and 180 kg N ha−1 in late season)as main plots,three seedling densities(16.7,75.0,and 187.5 seedlings m−2)as subplots,and three replications in the 2015 early and late seasons in Guangzhou,China.Light intensity at base of canopy(Lb),light quality as determined from red/far-red light ratio(R/FR),light transmission ratio(LTR),leaf area index(LAI),leaf N concentration(NLV)and final length of second internode(counted from soil surface upward)(FIL)were recorded.Higher N rate and seedling density resulted in significantly longer FIL.FIL was negatively correlated with Lb,LTR,and R/FR(P<0.01)and positively correlated with LAI(P<0.01),but not correlated with NLV(P>0.05).Stepwise linear regression analysis showed that FIL was strongly associated with Lb and LAI(R2=0.82).Heavy N application to pot-grown rice at the beginning of first internode elongation did not change FIL.We conclude that FIL is determined mainly by Lb and LAI at jointing stage.NLV has no direct effect on the elongation of basal internodes.N application indirectly affects FIL by changing LAI and light conditions in the rice canopy.Reducing LAI and improving canopy light transmission at jointing stage can shorten the basal internodes and increase the lodging resistance of rice.展开更多
Variations in leaf morphology and stomatal characteristics have been extensively studied at both inter- and intraspecific levels although not explicitly in the context of paper birch (Betula papyrifera Marsh) populati...Variations in leaf morphology and stomatal characteristics have been extensively studied at both inter- and intraspecific levels although not explicitly in the context of paper birch (Betula papyrifera Marsh) populations. The birch populations might have developed the leaf variations that allowed them to adapt to a wide climatic gradient. Therefore, in this study we examined variations in the leaf morphological and stomatal characteristics of sixteen paper birch populations collected across Canada and grown in a common garden. We also examined the relationship between these leaf characteristics and the climate of the population’s origin. Significant genotypic differences were found in the leaf characteristics measured among the birch populations. Thus, we expected that the observed leaf variations may be partly explained as natural diversity in the birch due to differences in environment of origin. We noticed that along mean annual precipitation and aridity gradients, hair density on leaf adaxial surface had decreased whereas stomatal density increased significantly. Our results showed that the populations with larger leaf area and specific leaf area had higher hair density but low stomatal density. These leaf characteristics provided a structural basis in reducing water loss through leaves and increasing water use efficiency. A trade-off between stomatal area and density resulted in this study might be a strategy of the birch to balance stomatal conductance in decreased precipitation.展开更多
Leaf morphological and anatomical structure and carbon isotope ratio (δ^13C) change with increasing tree height. To determine how tree height affects leaf characteristics, we measured the leaf area, specific leaf m...Leaf morphological and anatomical structure and carbon isotope ratio (δ^13C) change with increasing tree height. To determine how tree height affects leaf characteristics, we measured the leaf area, specific leaf mass (ratio of leaf mass to leaf area [LMA]), thickness of the total leaf, cuticle, epidermis, palisade and sponge mesophyll, stomata traits and δ^13C at different heights of Parashorea chinensis with methods of light and scanning electron microscopy (SEM) and isotope-ratio mass spectrometry. The correlation and stepwise regression between tree height and leaf structure traits were carried out with SPSS software. The results showed that leaf structures and δ^13C differed significantly along the tree height gradient. The leaf area, thickness of sponge mesophyll and size of stomata decreased with increasing height, whereas the thickness of lamina, palisade mesophyll, epidermis, and cuticle, ratios of palisade to spongy thickness, density of stomata and vascular bundles, LMA and δ^13C increased with tree height. Tree height showed a significant relationship with all leaf indices and the most significant relationship was with epidermis thickness, leaf area, cuticle thickness, δ^13C. The δ^13C value showed a significantly positive relationship with LMA (R = 0.934). Our results supported the hypothesis that the leaf structures exhibited more xeromorphic characteristics with the increasing gradient of tree height.展开更多
The competition-density (C-D) effects for mean mass for tree, stem, branch and leaf were analyzed in Acacia auriculiformis stands. Mean tree mass-density and mean organ mass-density were well explained by the C-D equa...The competition-density (C-D) effects for mean mass for tree, stem, branch and leaf were analyzed in Acacia auriculiformis stands. Mean tree mass-density and mean organ mass-density were well explained by the C-D equation of tree and the C-D equation of tree organ, respectively. An equation describing the relationship between mean leaf area u and density was formulated that fit the u-data well. The relationship between mean tree mass w and the ratio of each organ to mean tree mass (wo/ w) was examined. With increasing w, the stem mass ratio wS/w increased, whereas the branch mass ratio wB/w and the leaf mass ratio wL/w decreased. The yield difference between the lowest-density stand and the high-density stand became greater with stand growth. However, the yield of the mid-density stand was slightly lower than the yield of the high-density stand during the experimental period. To produce the most desirable combination of demanding individual-tree size and relative high stem yield, the mid-density is recommended as proper planting density for future management of A. auriculiformis stands.展开更多
Generally, plant species with shorter leaf longevity maintain a positive carbon balance by decreasing leaf mass per area (LMA) and increasing photosynthesis. However, plants at high elevations need to increase LMA aga...Generally, plant species with shorter leaf longevity maintain a positive carbon balance by decreasing leaf mass per area (LMA) and increasing photosynthesis. However, plants at high elevations need to increase LMA against environmental stresses. Therefore, plants need to increase both LMA and photosynthesis at high elevations. To examine how deciduous plants maintain a positive carbon balance at high elevations, photosynthesis and related leaf traits for deciduous broad-leaved tree Betula ermanii were measured at three elevations. LMA was greater at middle and high elevations than at low elevation. Leaf δ13C was greater at higher elevations, and positively correlated with LMA, indicating greater long-term deficiency of CO2 in leaves at higher elevations. However, the Ci/Ca ratio at photosynthetic measurement was not low at high elevations. Nitrogen content per leaf mass and stomatal conductance were greater at higher elevations. Photosynthetic rates and photosynthetic nitrogen use efficiency (PNUE) did not differ among the three elevations. Photosynthetic rate showed a strong positive correlation with stomatal conductance on a leaf area basis (R2 = 0.83, P < 0.001). Therefore, this study suggests B. ermanii compensates the deficiency of CO2 in leaves at high elevation by increasing stomatal conductance, and maintains photosynthesis and PNUE at high elevation as much as at low elevation.展开更多
Aims Subalpine coniferous species are distributed over a wide range of elevations in which they must contend with stressful conditions,such as high elevations and extended periods of darkness.Two evergreen coniferous ...Aims Subalpine coniferous species are distributed over a wide range of elevations in which they must contend with stressful conditions,such as high elevations and extended periods of darkness.Two evergreen coniferous species,Abies veitchii and Abies mariesii,dominate at low and high elevations,respectively,in the subalpine zone,central Japan.The aim of this study is to examine the effects of leaf age,elevation and light conditions on photosynthetic rates through changes in morphological and physiological leaf traits in the two species.Methods We here examined effects of leaf age,elevation and light conditions on photosynthesis,and leaf traits in A.veitchii and A.mariesii.Saplings of the two conifers were sampled in the understory and canopy gaps at their lower(1600 m)and upper(2300 m)distribution limits.Important Findings The two species showed similar responses to leaf age and different responses to elevation and light conditions in photosynthesis and leaf traits.The maximum photosynthetic rate of A.veitchii is correlated negatively with leaf mass per area(LMA)and non-structural carbohydrate(NSC)concentration.LMA increased at high elevations in the two species,whereas NSC concentrations increased only in A.veitchii.Therefore,the maximum photosynthetic rate of A.veitchii decreased at high elevations.Furthermore,maximum photosynthetic rates correlate positively with nitrogen concentration in both species.In the understory,leaf nitrogen concentrations decreased and increased in A.veitchii and A.mariesii,respectively.LMA decreased and the chlorophyll-to-nitrogen ratio increased in understory conditions only for A.mariesii,suggesting it has a higher light-capture efficiency in dark conditions than does A.veitchii.This study concluded that A.mariesii has more shade-tolerant photosynthetic and leaf traits and its photosynthetic rate is less affected by elevation compared with A.veitchii,allowing A.mariesii to survive in the understory and to dominate at high elevations.展开更多
基金Supported by the National Project of Transformation Fund for Agricultural Science and Technology Achievements:Pilot Production and Demonstration of New Wheat Variety and the Propagation of Breeder Seed(2013GB2D000300)The Special Fund of National Modern Agricultural Industry Technology System(CARS-03)~~
文摘To provide "more reasonable, more saving and more efficient" water and fertilizer application proposals, taking ‘Yujiao 5' as the experimental material, the effects of different irrigation times and nitrogen application treatments on the leaf area index and yield of wheat were studied using three-factor split plot method. The results showed that irrigation times, nitrogen application rate and the ratio of basa to topdressed nitrogen respectively had significant effects on the leaf area index, the yield and component factors of wheat. Under the treatment of W1(irrigation before sowing), the leaf area index showed a positive linear correlation with nitrogen application rate; under the treatments of W2(irrigation before sowing and at jointing stage) and W3(irrigation before sowing, at jointing stage and at grain filling stages),the leaf area index showed a positive linear correlation with nitrogen application rate at the jointing stage, booting stage and heading stage; 20 d after heading, the leaf area index showed a quadric curve relationship with nitrogen application rate at these stages, and the LAI of N3R2 was the highest. Under different irrigation times,the yield, ear number and kernels per ear showed quadric curve relationship with nitrogen application rate, 1 000-seed weight showed the trend of linear decrease with the increase of nitrogen application rate. Under the treatment combination of irrigation before sowing, at jointing stage and at grain filling stage, nitrogen application rate at 240 kg/hm^2 and the ratio of basal to topdressed nitrogen of 5:5, the grain yield(8 609.60 kg/hm^2), ear number(688.2×104/hm^2) and kernel number per ear(37.9 grains) reached the highest value at W3N3R2, and the grain yield of W3N3R2 increased by 144.8% compared to the W1N0. In conclusion, in Eastern Henan where the rainfall is insufficient at the late growth stage of wheat, the irrigation-saving space in wheat production is relatively small, but the nitrogen-saving space is relatively large.
基金supported by the Natural Science Foundation of Guangdong Province,China(S2012020011043)the National High Technology Research and Development Program of China(2014AA10A605)+2 种基金the Special Fund for Agro-scientific Research in the Public Interest(201503106)Modern Agriculture Industry Technology System for Rice in Guangdong Province(2016LM1066,2017LM1066,2018LM1066)the Swiss Agency for Development and Cooperation through its funding of “Closing Rice Yield Gaps in Asia” Project(CORIGAP)
文摘Short basal internodes are important for lodging resistance of rice(Oryza sativa L.).Several canopy indices affect the elongation of basal internodes,but uncertainty as to the key factors determining elongation of basal internodes persists.The objectives of this study were(1)to identify key factors affecting the elongation of basal internodes and(2)to establish a quantitative relationship between basal internode length and canopy indices.An inbred rice cultivar,Yinjingruanzhan,was grown in two split-plot field experiments with three N rates(0,75,and 150 kg N ha−1 in early season and 0,90,and 180 kg N ha−1 in late season)as main plots,three seedling densities(16.7,75.0,and 187.5 seedlings m−2)as subplots,and three replications in the 2015 early and late seasons in Guangzhou,China.Light intensity at base of canopy(Lb),light quality as determined from red/far-red light ratio(R/FR),light transmission ratio(LTR),leaf area index(LAI),leaf N concentration(NLV)and final length of second internode(counted from soil surface upward)(FIL)were recorded.Higher N rate and seedling density resulted in significantly longer FIL.FIL was negatively correlated with Lb,LTR,and R/FR(P<0.01)and positively correlated with LAI(P<0.01),but not correlated with NLV(P>0.05).Stepwise linear regression analysis showed that FIL was strongly associated with Lb and LAI(R2=0.82).Heavy N application to pot-grown rice at the beginning of first internode elongation did not change FIL.We conclude that FIL is determined mainly by Lb and LAI at jointing stage.NLV has no direct effect on the elongation of basal internodes.N application indirectly affects FIL by changing LAI and light conditions in the rice canopy.Reducing LAI and improving canopy light transmission at jointing stage can shorten the basal internodes and increase the lodging resistance of rice.
文摘Variations in leaf morphology and stomatal characteristics have been extensively studied at both inter- and intraspecific levels although not explicitly in the context of paper birch (Betula papyrifera Marsh) populations. The birch populations might have developed the leaf variations that allowed them to adapt to a wide climatic gradient. Therefore, in this study we examined variations in the leaf morphological and stomatal characteristics of sixteen paper birch populations collected across Canada and grown in a common garden. We also examined the relationship between these leaf characteristics and the climate of the population’s origin. Significant genotypic differences were found in the leaf characteristics measured among the birch populations. Thus, we expected that the observed leaf variations may be partly explained as natural diversity in the birch due to differences in environment of origin. We noticed that along mean annual precipitation and aridity gradients, hair density on leaf adaxial surface had decreased whereas stomatal density increased significantly. Our results showed that the populations with larger leaf area and specific leaf area had higher hair density but low stomatal density. These leaf characteristics provided a structural basis in reducing water loss through leaves and increasing water use efficiency. A trade-off between stomatal area and density resulted in this study might be a strategy of the birch to balance stomatal conductance in decreased precipitation.
基金the National Natural Science Foundation of China (30070637)Beijing Municipal Science & Technology Commission Project "Drought re-sistant and water-saving species selection and application for Beijing urbangreenism" (D0605001040191)the Key Laboratory for Silvicuture andConservation, Ministry of Education Project "Water economic ecology re-search for Beijing urban greenbelt" (JD100220535).
文摘Leaf morphological and anatomical structure and carbon isotope ratio (δ^13C) change with increasing tree height. To determine how tree height affects leaf characteristics, we measured the leaf area, specific leaf mass (ratio of leaf mass to leaf area [LMA]), thickness of the total leaf, cuticle, epidermis, palisade and sponge mesophyll, stomata traits and δ^13C at different heights of Parashorea chinensis with methods of light and scanning electron microscopy (SEM) and isotope-ratio mass spectrometry. The correlation and stepwise regression between tree height and leaf structure traits were carried out with SPSS software. The results showed that leaf structures and δ^13C differed significantly along the tree height gradient. The leaf area, thickness of sponge mesophyll and size of stomata decreased with increasing height, whereas the thickness of lamina, palisade mesophyll, epidermis, and cuticle, ratios of palisade to spongy thickness, density of stomata and vascular bundles, LMA and δ^13C increased with tree height. Tree height showed a significant relationship with all leaf indices and the most significant relationship was with epidermis thickness, leaf area, cuticle thickness, δ^13C. The δ^13C value showed a significantly positive relationship with LMA (R = 0.934). Our results supported the hypothesis that the leaf structures exhibited more xeromorphic characteristics with the increasing gradient of tree height.
基金supported by the Forestry Technology Popularization Demonstration Project of the Central Government of China(No.[2015]GDTK-07)
文摘The competition-density (C-D) effects for mean mass for tree, stem, branch and leaf were analyzed in Acacia auriculiformis stands. Mean tree mass-density and mean organ mass-density were well explained by the C-D equation of tree and the C-D equation of tree organ, respectively. An equation describing the relationship between mean leaf area u and density was formulated that fit the u-data well. The relationship between mean tree mass w and the ratio of each organ to mean tree mass (wo/ w) was examined. With increasing w, the stem mass ratio wS/w increased, whereas the branch mass ratio wB/w and the leaf mass ratio wL/w decreased. The yield difference between the lowest-density stand and the high-density stand became greater with stand growth. However, the yield of the mid-density stand was slightly lower than the yield of the high-density stand during the experimental period. To produce the most desirable combination of demanding individual-tree size and relative high stem yield, the mid-density is recommended as proper planting density for future management of A. auriculiformis stands.
文摘Generally, plant species with shorter leaf longevity maintain a positive carbon balance by decreasing leaf mass per area (LMA) and increasing photosynthesis. However, plants at high elevations need to increase LMA against environmental stresses. Therefore, plants need to increase both LMA and photosynthesis at high elevations. To examine how deciduous plants maintain a positive carbon balance at high elevations, photosynthesis and related leaf traits for deciduous broad-leaved tree Betula ermanii were measured at three elevations. LMA was greater at middle and high elevations than at low elevation. Leaf δ13C was greater at higher elevations, and positively correlated with LMA, indicating greater long-term deficiency of CO2 in leaves at higher elevations. However, the Ci/Ca ratio at photosynthetic measurement was not low at high elevations. Nitrogen content per leaf mass and stomatal conductance were greater at higher elevations. Photosynthetic rates and photosynthetic nitrogen use efficiency (PNUE) did not differ among the three elevations. Photosynthetic rate showed a strong positive correlation with stomatal conductance on a leaf area basis (R2 = 0.83, P < 0.001). Therefore, this study suggests B. ermanii compensates the deficiency of CO2 in leaves at high elevation by increasing stomatal conductance, and maintains photosynthesis and PNUE at high elevation as much as at low elevation.
基金supported by grants(20292081)from the Ministry of Education,Culture,Sports,Science and Technology,Japan.
文摘Aims Subalpine coniferous species are distributed over a wide range of elevations in which they must contend with stressful conditions,such as high elevations and extended periods of darkness.Two evergreen coniferous species,Abies veitchii and Abies mariesii,dominate at low and high elevations,respectively,in the subalpine zone,central Japan.The aim of this study is to examine the effects of leaf age,elevation and light conditions on photosynthetic rates through changes in morphological and physiological leaf traits in the two species.Methods We here examined effects of leaf age,elevation and light conditions on photosynthesis,and leaf traits in A.veitchii and A.mariesii.Saplings of the two conifers were sampled in the understory and canopy gaps at their lower(1600 m)and upper(2300 m)distribution limits.Important Findings The two species showed similar responses to leaf age and different responses to elevation and light conditions in photosynthesis and leaf traits.The maximum photosynthetic rate of A.veitchii is correlated negatively with leaf mass per area(LMA)and non-structural carbohydrate(NSC)concentration.LMA increased at high elevations in the two species,whereas NSC concentrations increased only in A.veitchii.Therefore,the maximum photosynthetic rate of A.veitchii decreased at high elevations.Furthermore,maximum photosynthetic rates correlate positively with nitrogen concentration in both species.In the understory,leaf nitrogen concentrations decreased and increased in A.veitchii and A.mariesii,respectively.LMA decreased and the chlorophyll-to-nitrogen ratio increased in understory conditions only for A.mariesii,suggesting it has a higher light-capture efficiency in dark conditions than does A.veitchii.This study concluded that A.mariesii has more shade-tolerant photosynthetic and leaf traits and its photosynthetic rate is less affected by elevation compared with A.veitchii,allowing A.mariesii to survive in the understory and to dominate at high elevations.