A novel approach to virus-induced post-transcriptional gene silencing for studying the function of the ribulose bisphosphate carboxylase small subunit （rbcS） gene was established and optimized using potato virus X v...A novel approach to virus-induced post-transcriptional gene silencing for studying the function of the ribulose bisphosphate carboxylase small subunit （rbcS） gene was established and optimized using potato virus X vector and Nicotiana benthamiana as experimental material. The analysis of silencing phenomena, transcriptional level, protein expression, and pigment measurement showed that the expression of the rbcS endogenous gene was inactivated by the expression of a 500-bp homologous cDNA fragment carried in the virus vector.展开更多
基金Supported by the National Natural Science Foundation of China, the Chinese National Program for Changjiang Scholars and Innovative Research Team （PCSIRT） in University （#1RT0519）, Plant Transformation Center and Jilin Provincial Science and Technology Commission. Acknowledgements We thank Dr David Baulcombe, Sainsbury Lab, UK, for providing the PVX vector construct and A.GV3101, and Dr Rachel A. Burton, Department of Plant Science, University of Adelaide, Australia, for providing the IV. benthamiana seeds.
文摘A novel approach to virus-induced post-transcriptional gene silencing for studying the function of the ribulose bisphosphate carboxylase small subunit （rbcS） gene was established and optimized using potato virus X vector and Nicotiana benthamiana as experimental material. The analysis of silencing phenomena, transcriptional level, protein expression, and pigment measurement showed that the expression of the rbcS endogenous gene was inactivated by the expression of a 500-bp homologous cDNA fragment carried in the virus vector.
