The alcohol and n-butanol extract of Potentilla anserine L. significantly protects myocardium from acute ischemic injury. However, its effects on rat hippocampal neurons and the mechanism of protection remain unclear....The alcohol and n-butanol extract of Potentilla anserine L. significantly protects myocardium from acute ischemic injury. However, its effects on rat hippocampal neurons and the mechanism of protection remain unclear. In this study, primary cultured hippocampal neurons from neonatal rats were incubated in 95% N2 and 5% CO2 for 4 hours. Results indicated that hypoxic injury decreased the viability of neurons, increased the expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein. Pretreatment with 0.25, 0.062 5, 0.015 6 mg/mL n-butanol extract of Potentilla anserine L. led to a significant increase in cell viability. Expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein, were attenuated. The neuroprotective effect of n-butanol extract of Potentilla anserine L. was equivalent to tanshinone IIA. Our data suggest that the n-butanol extract of Potentilla anserine L. could protect primary hippocampal neurons from hypoxic injury by deactivating mitochondrial cell death.展开更多
The study of the water status and activity of catalase in Chinese tea plants (Camellia sinensis (L.) O. Kuntze) and hazelnut (Corylus pontica C. Koch.). The indicators, which not only describe the physiological state ...The study of the water status and activity of catalase in Chinese tea plants (Camellia sinensis (L.) O. Kuntze) and hazelnut (Corylus pontica C. Koch.). The indicators, which not only describe the physiological state of plants in the stressful period, but also allow it to identify more adaptive varieties within a given area was determined. Analysis of catalase activity data in Chinese tea and hazelnut revealed the presence of similar patterns: there is a change in enzymatic activity in response to hydrothermal factors. It is established that the stable varieties Camellia sinensis (L.) O. Kuntze and Corylus pontica C. Koch. characterized by a more active catalase, which is of particular importance during droughts. The water regime parameters variance scale for Camellia sinensis was developed, which allows differentiating varieties into groups of varying degrees of stability. It is shown that all methods that are used to estimate stability of plant species to extreme environmental conditions are based on several basic principles connected with peculiarities of adaptation mechanisms. The result was a selection of diagnostic indicators proposed for assessing adaptability: the water content of the leaf tissue, water-holding capacity of leaves, and concentration of cellular juice sprouts and activity of catalase.展开更多
Objective:To explore the application effect of constructing professional teaching staff in low-level training in operating room,so as to further optimize the teaching strength of operating room in our hospital and imp...Objective:To explore the application effect of constructing professional teaching staff in low-level training in operating room,so as to further optimize the teaching strength of operating room in our hospital and improve the training effect of junior nurses.Methods:Thirty-eight low-level nurses in the operating room of a third-class hospital in Yantai were selected for half a year's nurse training.With theoretical scores,overall nursing performance,nurses'self-awareness evaluation system and nurses'satisfaction with tutors as evaluation criteria,and based on the selection of high-quality teachers,various evaluation indexes before and after the training of low-level nurses in the operating room were compared and evaluated through the cultivation of practical teaching teachers'ability and the application of a series of teaching methods based on the change of c ompetence-based education(CBE)teaching mode,the application of guided learning interactive canadian education(BOPPPS)teaching model and p roblem-b ased l earning(PBL)teaching method.Results:After the training,the examination scores of low-level nurses were significantly improved(P<0.05),the teaching quality was highly recognized by low-level nurses,the quality of low-level nurses was improved,and patients'satisfaction with nurses was improved.Conclusion:It is of great significance to assist the construction of professional teachers in evidence-based medicine.Through the training of practical teaching teachers'ability and the application of a series of teaching methods based on the change of CBE teaching model,the application of BOPPPS teaching model and PBL teaching method,the training results of nurses have been significantly improved and improved,which is worthy of clinical reference and promotion.展开更多
The oat panicle components were changed by the forms of nitrogen use influencing productivity. The aim of the study was to determine the direct and indirect effects of oat panicle components on grain yield by N-fertil...The oat panicle components were changed by the forms of nitrogen use influencing productivity. The aim of the study was to determine the direct and indirect effects of oat panicle components on grain yield by N-fertilizer rates in succession systems of high and reduced release of N-residual. The study was conducted in the years 2013 and 2014 in a randomized complete block with four replications in a factorial 3 × 2, for nitrogen rates (30, 60 and 120 kg·ha<sup>-1</sup>) and oat cultivars (Barbarasul and Brisasul) the soybean/oat and corn/oat systems. The panicle weight and grain per panicle show greater sensitivity to the change of nitrogen rates. Grain yield was favored by the direct effect of harvest index panicle and indirect by grain weight per panicle. In the system soybean/ oat, the grain weight of panicle shows greater efficiency in promoting positive direct effects on grain yield.展开更多
A comparison of sensitivity in extratropical circulation in the Northern Hemisphere(NH)and Southern Hemisphere(SH)is conducted through observational analyses and diagnostic linear model experiments for two types of El...A comparison of sensitivity in extratropical circulation in the Northern Hemisphere(NH)and Southern Hemisphere(SH)is conducted through observational analyses and diagnostic linear model experiments for two types of El Nio events,the traditional El Nio with the strongest warmth in the eastern tropical Pacific(EP El Nio)and the El Nio Modoki with the strongest warmth in the central tropical Pacific(CP El Nio).It is shown that CP El Nio favors the occurrence of a negative-phase Northern Annular Mode(NAM),while EP El Nio favors that of the Pacific-North American(PNA)pattern.In SH,both EP and CP El Nio induce a negative phase Southern Annular Mode(SAM).However,the former has a greater amplitude,which is consistent with the stronger sea surface temperature(SST)warmth.The difference in the two types of El Nio events in NH may originate from the dependence of heating-induced extratropical response on the location of initial heating,which may be associated with activity of the stationary wave.In SH,the lack of sensitivity to the location of heating can be associated with weaker activity of the stationary wave therein.展开更多
A new efficient method for preparing o-L-α-glycerylphosphoryl-L-serine was presented. D-α, β-isopropylidene glycerol was phosphorylated with phenylphosphoryl dichloride and the resulting o-D-α,β-isopropylidene gl...A new efficient method for preparing o-L-α-glycerylphosphoryl-L-serine was presented. D-α, β-isopropylidene glycerol was phosphorylated with phenylphosphoryl dichloride and the resulting o-D-α,β-isopropylidene glycerylphenylphosphoryl chloride was esterified with N-tert-butoxycarbonyl-L-serine ethyl ester in the presence of pyridine to give acetone L-α-glycerylphenylphosphoryl-N-tert-butoxycarbonyl-L-serine ethyl ester. Finally, the protective groups were removed by two-step hydrolysis while strictly controlling pH value. The reaction to produce (1, 2), (5, 6)-diisopropylidene-D-mannitol, which is the key precursor for the preparation of D-α, β-isopropylidene glycerol, was monitored by using gas chromatography-mass spectrometer in order to obtain an optimum yield of 65%. All compounds were characterized through elemental analysis, infrared spectra, 1H, 13C, 31P-nuclear magnetic resonance spectra and mass spectra.展开更多
基金supported by the National Natural Science Foundation of China, No. 30672774 and No. 81073152the Great Program of Science Foundation of Tianjin, No.10JCZDJC21100
文摘The alcohol and n-butanol extract of Potentilla anserine L. significantly protects myocardium from acute ischemic injury. However, its effects on rat hippocampal neurons and the mechanism of protection remain unclear. In this study, primary cultured hippocampal neurons from neonatal rats were incubated in 95% N2 and 5% CO2 for 4 hours. Results indicated that hypoxic injury decreased the viability of neurons, increased the expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein. Pretreatment with 0.25, 0.062 5, 0.015 6 mg/mL n-butanol extract of Potentilla anserine L. led to a significant increase in cell viability. Expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein, were attenuated. The neuroprotective effect of n-butanol extract of Potentilla anserine L. was equivalent to tanshinone IIA. Our data suggest that the n-butanol extract of Potentilla anserine L. could protect primary hippocampal neurons from hypoxic injury by deactivating mitochondrial cell death.
文摘The study of the water status and activity of catalase in Chinese tea plants (Camellia sinensis (L.) O. Kuntze) and hazelnut (Corylus pontica C. Koch.). The indicators, which not only describe the physiological state of plants in the stressful period, but also allow it to identify more adaptive varieties within a given area was determined. Analysis of catalase activity data in Chinese tea and hazelnut revealed the presence of similar patterns: there is a change in enzymatic activity in response to hydrothermal factors. It is established that the stable varieties Camellia sinensis (L.) O. Kuntze and Corylus pontica C. Koch. characterized by a more active catalase, which is of particular importance during droughts. The water regime parameters variance scale for Camellia sinensis was developed, which allows differentiating varieties into groups of varying degrees of stability. It is shown that all methods that are used to estimate stability of plant species to extreme environmental conditions are based on several basic principles connected with peculiarities of adaptation mechanisms. The result was a selection of diagnostic indicators proposed for assessing adaptability: the water content of the leaf tissue, water-holding capacity of leaves, and concentration of cellular juice sprouts and activity of catalase.
文摘Objective:To explore the application effect of constructing professional teaching staff in low-level training in operating room,so as to further optimize the teaching strength of operating room in our hospital and improve the training effect of junior nurses.Methods:Thirty-eight low-level nurses in the operating room of a third-class hospital in Yantai were selected for half a year's nurse training.With theoretical scores,overall nursing performance,nurses'self-awareness evaluation system and nurses'satisfaction with tutors as evaluation criteria,and based on the selection of high-quality teachers,various evaluation indexes before and after the training of low-level nurses in the operating room were compared and evaluated through the cultivation of practical teaching teachers'ability and the application of a series of teaching methods based on the change of c ompetence-based education(CBE)teaching mode,the application of guided learning interactive canadian education(BOPPPS)teaching model and p roblem-b ased l earning(PBL)teaching method.Results:After the training,the examination scores of low-level nurses were significantly improved(P<0.05),the teaching quality was highly recognized by low-level nurses,the quality of low-level nurses was improved,and patients'satisfaction with nurses was improved.Conclusion:It is of great significance to assist the construction of professional teachers in evidence-based medicine.Through the training of practical teaching teachers'ability and the application of a series of teaching methods based on the change of CBE teaching model,the application of BOPPPS teaching model and PBL teaching method,the training results of nurses have been significantly improved and improved,which is worthy of clinical reference and promotion.
文摘The oat panicle components were changed by the forms of nitrogen use influencing productivity. The aim of the study was to determine the direct and indirect effects of oat panicle components on grain yield by N-fertilizer rates in succession systems of high and reduced release of N-residual. The study was conducted in the years 2013 and 2014 in a randomized complete block with four replications in a factorial 3 × 2, for nitrogen rates (30, 60 and 120 kg·ha<sup>-1</sup>) and oat cultivars (Barbarasul and Brisasul) the soybean/oat and corn/oat systems. The panicle weight and grain per panicle show greater sensitivity to the change of nitrogen rates. Grain yield was favored by the direct effect of harvest index panicle and indirect by grain weight per panicle. In the system soybean/ oat, the grain weight of panicle shows greater efficiency in promoting positive direct effects on grain yield.
基金supported by the National Natural Science Foundation of China(41205048)the National Basic Research Program of China,"Structures,Variability and Climatic Impacts of Ocean Circulation and Warm Pool in the Tropical Pacific Ocean"(2012CB417403)the Special Project of Chinese Academy of Sciences(XDA11010401)
文摘A comparison of sensitivity in extratropical circulation in the Northern Hemisphere(NH)and Southern Hemisphere(SH)is conducted through observational analyses and diagnostic linear model experiments for two types of El Nio events,the traditional El Nio with the strongest warmth in the eastern tropical Pacific(EP El Nio)and the El Nio Modoki with the strongest warmth in the central tropical Pacific(CP El Nio).It is shown that CP El Nio favors the occurrence of a negative-phase Northern Annular Mode(NAM),while EP El Nio favors that of the Pacific-North American(PNA)pattern.In SH,both EP and CP El Nio induce a negative phase Southern Annular Mode(SAM).However,the former has a greater amplitude,which is consistent with the stronger sea surface temperature(SST)warmth.The difference in the two types of El Nio events in NH may originate from the dependence of heating-induced extratropical response on the location of initial heating,which may be associated with activity of the stationary wave.In SH,the lack of sensitivity to the location of heating can be associated with weaker activity of the stationary wave therein.
文摘A new efficient method for preparing o-L-α-glycerylphosphoryl-L-serine was presented. D-α, β-isopropylidene glycerol was phosphorylated with phenylphosphoryl dichloride and the resulting o-D-α,β-isopropylidene glycerylphenylphosphoryl chloride was esterified with N-tert-butoxycarbonyl-L-serine ethyl ester in the presence of pyridine to give acetone L-α-glycerylphenylphosphoryl-N-tert-butoxycarbonyl-L-serine ethyl ester. Finally, the protective groups were removed by two-step hydrolysis while strictly controlling pH value. The reaction to produce (1, 2), (5, 6)-diisopropylidene-D-mannitol, which is the key precursor for the preparation of D-α, β-isopropylidene glycerol, was monitored by using gas chromatography-mass spectrometer in order to obtain an optimum yield of 65%. All compounds were characterized through elemental analysis, infrared spectra, 1H, 13C, 31P-nuclear magnetic resonance spectra and mass spectra.
基金supported by the National Natural Science Foundation of China(21002006,20452002)Special Program for Key Basic Research of the Ministry of Science and Technology,China(2004-973-36)~~
基金supported by the National Natural Science Foundation of China(21002006,20452002)Special Program for Key Basic Research of the Ministry of Science and Technology,China(2004-973-36)~~
