Isolation and Identification of a Specific cDNA Mapping to the Bam HI-I2 and -LFragments within the Inverted Repeats ofUnique Long Re-gion (IRL) in the Genom e ofMarek′s Disease Herpesvirus (MDV) Oncogenic Strain Beijing-1

Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.

公共服务的空间叙事:可及性、可及化与可及度

公共服务是空间的存在。向民众提供可及的公共服务,离不开相应的“空间中的生产”和“空间的生产”。可及性是空间权利的具体质性和空间权力运行导向,是供需适配的空间结构。可及化是公共服务空间在建构理性与进化理性、政府规划与公众参与、政治势能与社会动能相统一的基础上,发挥激活、整合、链接三种机制功能作用的实践过程。可及度是衡量空间可及性的主体性尺度,具体包括节点利用率、连线密切度、曲面平滑度三个方面。空间是物质广延性与思维能动性的统一,同质性与多样性的统一。从政治社会空间来诠释公共服务,使公平的价值准则转化为具体的可及权利,可以进一步拓展公共服务的分析视域。

Water Quality Analysis of the Songhua River Basin Using Multivariate Techniques

Multivariate statistical techniques, including cluster analysis (CA), principal component analysis (PCA), factor analysis (FA) and discriminant analysis (DA), were used to evaluate temporal and spatial variations and to interpret a large and complex water quality data sets collected from the Songhua River Basin. The data sets, which contained 14 parameters, were generated during the 7-year (1998-2004) monitoring program at 14 different sites along the rivers. Three significant sampling locations (less polluted sites, moderately polluted sites and highly polluted sites) were detected by CA method, and five latent factors (organic, inor-ganic, petrochemical, physiochemical, and heavy metals) were identified by PCA and FA methods. The re-sults of DA showed only five parameters (temperature, pH, dissolved oxygen, ammonia nitrogen, and nitrate nitrogen) and eight parameters (temperature, pH, dissolved oxygen, biochemical oxygen demand, ammonia nitrogen, nitrate nitrogen, volatile phenols and total arsenic) were necessarily in temporal and spatial varia-tions analysis, respectively. Furthermore, this study revealed the major causes of water quality deterioration were related to inflow of effluent from domestic and industrial wastewater disposal.

关于控制受限下系统的可控、可达性

这里所探讨的问题是离散线性系统的多变量受限控制问题。对于系统 X_(k+1)=AX_k+BU_k U_k∈Ω■ R^m给出了系统可达的充要条件。在此基础上又给出了4个推论:l°当A为正数矩阵,且A的复特征根为单根时,系统区域可达的充要条件。2°系统区域零可控的充要条件。3°A^(-1)满足一定的条件时,系统区域零可控的充要条件。4°当Ω=R_+~m时,系统整体可达的充要条件。

Thermoelastic Stability of Closed Cylindrical Shell in Supersonic Gas Flow

In a linear framework,the problem of stability of closed cylindrical shell is briefly discussed.The cylindrical shell is immersed in a supersonic gas flow and under the influence of temperature field varying along the thickness.An unperturbed uniform velocity flow field,directed along the short edges of the shell,is applied.Due to the inhomogeneity of the temperature field distribution across the thickness shell buckling instability occurs.This instability accounts for the deformed shape of the shell,to be referred as the unperturbed state.Stability conditions and boundary for the unperturbed state of the system under consideration are presented following the basic theory of aero-thermo-elasticity.The stability boundary depends on the variables characterizing the flow speed,the temperature at the middle plane of the shell and the temperature gradient in the direction normal to that plane.It is shown that the combined effect of the temperature field and flowing stream regulates the process of stability,and the temperature field can significantly change the flutter critical speed.

Creating Regional Tourism Standards in the Yangtze River Dalta Region

With the strategicdevelopment of regionaltourism cooperation in theYangtze River Delta region,acceleratingthe creation oftourism standards becomesparamount.Which organizationcould best develop tourismstandards and the principles oftourism standards are discussedin this paper.Thelong-term and short-term plansfor the creation of regionaltourism standards in theYangtze River Delta region arealso presented.

Runoff Variation Law and Its Response to Climate Change in the Headstream Area of the Keriya River Basin,Xinjiang

Based on the runoff and meteorological data of Langan（兰干） Hydrological Station from 1957 to 2009 in Keriya（克里雅） River,the periodicities,abrupt changes,and trends of climate factors and runoff were investigated by wavelet analysis and nonparametric test;then,the future change of the annual runoff was predicted by a periodic trend superposition model.In succession,the influencing volumes of climate change on the annual runoff were separated from the observation values of the an-nual runoff in Keriya River.The results show that（1） temperature series increased significantly,while the annual runoff and precipitation of Keriya River increased insignificantly at the significant level of α=0.05;（2） the common periods of 9 and 15 years existed in the annual runoff evolution process,and the primary periods of temperature and precipitation were 9 and 22 years and 9 and 13 years,respec-tively;（3） the annual runoff did not vary simultaneously with the abrupt change of climate factors in the headstream;the abrupt points of annual runoff and temperature are at 1998 and 1980 year,and that of precipitation is not so significant;and（4） the annual runoff will experience a decrease trend in the future period;the total increasing volume owing to climate change is 23.154×108 m3 in the head-stream during the period of 1999-2009;however,the stream flow has been nearly utilized completely due to the human activities in the mainstream area of Keriya River.

轨道公交站台信息传达的通用设计研究

本文以轨道公交站台中的信息传达设计进行展开,以满足公民最基本的平等出行、社交的权利为出发点,结合通用设计理论的核心价值,把握其在现代工业设计中以“如何能被功能有障碍的人使用,就更能被所有的人使用”⑴的设计准则,引入到我国城市轨道公交站台的信息传达设计之中。