The Study on the Correlation Between Plasma Adiponectin Level and Coronary Heart Disease

Objectives To investigate the relationship between plasma adiponectin level and coronary heart disease （CHD）, and some established cardiovascular risk factors and to probe its probable pathogenesis which adiponectin results in CHD. Methods The levels of plasma adiponectin, fasting plasma insulin （FINS）, C-reactive protein （CRP） and P-selectin were measured by ELISA, plasma ET-1 was measured by radioimmunoassay （RIA） in 75 male patients with CHD and 30 healthy male people. Body mass index （BMI）, waist / hip ratio （WHR） and insulin resistance index （Homa-IR） were calculated respectively. Results （1）The plasma adiponectin levels in CHD group were lower compared with control group[（5.18±2.57）mg / L vs（8.94±2.59）mg / L, P〈 0.001 ], there was no significant difference of plasma adiponectin levels in CHD sub-groups （P 〉 0.05）.（2） Based on multinominal stepwise logistic regression analysis, adiponectin was one of significant and independent risk factors for CHD. （3） Multivariate liner stepwise regression analysis showed that adiponectin had significant correlation with BMI and TG, BMI and TG were independent factors influencing on plasma adiponectin levels. （4） Pearson correlation analysis indicated plasma adiponectin levels were inversely related to FINS levels , Homa-IR, CRP, P-selectin and ET-1. Conclusions （ 1 ）Plasma adiponectin levels are lower in CHD patients compared the control subjects, there are no significant difference of plasma adiponectin levels in patients with SAP, UAP and AMI. （2） Plasma adiponectin levels are relative with CHD. Hypoadiponectinemia is an independent risk factor for CHD. （3）Established cardiovascular risk factors such as BMI and TG have an obvious influence on adiponectin. （4）The probable pathogenesis by which adiponectin involves in CHD is suggested that adiponectin relates to insulin resistance, inflammatory reaction and dysfunction of vessel endothelium.

ACTIVATION OF BOVINE FACTOR IX BY THE REACTION PRODUCT OF BOVINE FACTOR VII AND HUMAN TISSUE FACTOR

A study was carried out on the alternate activation of factor IX （FIX） by bovine FVII and human tissue factor （TF） rather than by activated factor XI （FXI）. The reaction product of bovine FVII and human TF functioned as a FIX activator in the assay system used. Published studies suggest that in the presence of Caions, the complex of human FVII-TF readily activates both human FIX and human FX,and at low TF concentrations, FIX appears to be the preferred substrate for the reaction product of FVII and TF. This may explain the discrepancy between the mild bleeding of hereditary FXI deficiency and the severe bleeding of hereditery FIX deficieney. The results obtained with bovine FVII and a crude human TF preparation confirm that at low TF concentrations,bovine FIX is the preferred substrate rather than FX. At higher TF concentrations, bovine FX was rapidly activated.

Thermonuclear ^19F(p,α0)^16O reaction rate

The thermonuclear^19F（p,α0）16O reaction rate in the temperature region 0.007–10 GK has been derived by re-evaluating the available experimental data, together with the low-energy theoretical R-matrix extrapolations.Our new rate deviates by up to about 30% compared to the previous results, although all rates are consistent within the uncertainties. At very low temperature（e.g. 0.01 GK） our reaction rate is about 20% lower than the most recently published rate, because of a difference in the low energy extrapolated S-factor and a more accurate estimate of the reduced mass used in the calculation of the reaction rate. At temperatures above ^1 GK, our rate is lower, for instance, by about 20% around 1.75 GK, because we have re-evaluated the previous data（Isoya et al., Nucl. Phys.7, 116（1958）） in a meticulous way. The present interpretation is supported by the direct experimental data. The uncertainties of the present evaluated rate are estimated to be about 20% in the temperature region below 0.2 GK,and are mainly caused by the lack of low-energy experimental data and the large uncertainties in the existing data.Asymptotic giant branch（AGB） stars evolve at temperatures below 0.2 GK, where the^19F（p,α）16O reaction may play a very important role. However, the current accuracy of the reaction rate is insufficient to help to describe, in a careful way, the fluorine over-abundances observed in AGB stars. Precise cross section（or S factor） data in the low energy region are therefore needed for astrophysical nucleosynthesis studies.

Modeling Car-Following Dynamics During the Starting and Stopping Process Based on a Spring System Model

Car-following models describe how one vehicle follows the preceding vehicles.ln order to better model and explain car-following dynamics,this paper categorizes the state of a traveling vehicle into three sub-processes:the starting(acceleration)process,the car-folloing process,and the stopping(deceleration)process.The stating process primarily involves vehicle acceleration behavior.The stopping process involves not only car-following behavior but also deceleration behavior.This paper regards both the stopping process and the starting process as spring systems.The car-following dynamics during the starting process and the stopping process is modeled in this paper.The parameters of the proposed models,which are represented in the form of trigonometric functions,possess explicit hysical meaning and definitive ranges.We have calibrated the model of the starting process using data form the Traffic Engineering Handbook and ob-tained reasonable results.Compared with traditional stimulus-response car-following models,this model can better explain traffic flow phenomena and driver behavior thery.

PCR assay for the inversion causing severe Hemophilia A and its application

Objective To develop a new technique based on long distance polymerase chain reaction (LD PCR) to replace Southern blotting method to detect Factor Ⅷ (FⅧ) gene inversion leading to severe Hemophilia A (HA) and carrier.Methods Four primers P, Q, A&B were designed and synthesized. P&Q is sp ecific for 5' and 3' flanking regions of F8A 1 respectively. A&B is specific fo r 5' and 3' flanking regions of F8A 2/F8A 3 respectively. LD PCR with 3 p rime rs and 3 temprature was set up, optimized and used to detect the inversion.Results The LD PCR with primers P,Q,A&B, P,Q&B and P,Q&A can be used t o detect the gene inversion and discriminate carrier from wild type. A blind ana lysis of 53 DNA samples from HA families was carried out by the LD PCR and Sout hern blotting respectively. Two sets of the results were completely identical. T hey were 23 cases of inversion, 27 cases of wild type and 3 cases of carriers. T he sensitivity and specificity of LD PCR are both 100%. Three inversion hem izygotes and 4 female carriers were identified from 5 HA families by the LD PCR technology.Conclusions The LD PCR with primer P,Q&B or P,Q,A&B can be used to det ect the gene inversion and the carrier of inversion. Compared with Southern blot ting, this technique is simple, rapid, inexpensive, more sensitive, accurate and non isotopic.

Aberrant expression of growth factor Wnt-5A in six urinary malignant cell lines

Objective To investigate the expression of growth factor Wnt 5A in urinary tumor cell lines. Methods By semi quantitative RT PCR (reverse transcriptase polymerase chain reaction), the expression of Wnt 5A in six urinary malignant cell lines, one primary cultured renal fibroblast and one case of normal human renal tissue was detected using Gel Doc 1000 computer controlled molecular analysis system. Results The expression of Wnt 5A in urinary tumor cell lines was much higher than that in primary cultured renal fibroblasts (PRF) and normal human renal tissue (NRT). The levels of Wnt 5A mRNA were different between six malignant cell lines. Conclusion The overexpression of growth factor Wnt 5A has a potential effect on the oncogenesis of urinary malignancies.