The efficiency of TFMSA as a cleaving reagent for peptidyl resin(I-VIII)was evaluated by quantitative ninhydrin test of peptide substitution degree(SD)on resin.Under different conditions,such as variation of C-termina...The efficiency of TFMSA as a cleaving reagent for peptidyl resin(I-VIII)was evaluated by quantitative ninhydrin test of peptide substitution degree(SD)on resin.Under different conditions,such as variation of C-terminal residue,resin linkage,the temperature and reaction time etc.,TFMSA can be used very conveniently instead of HF in many cases.展开更多
We report here the synthesis of cyclic peptides using benzotriazolyloxy-bis(pyrrolidino)-carbonium hexafluoro- phosphate(BBC)as a coupling reagent with high yield and speed even in low concentration compared with the ...We report here the synthesis of cyclic peptides using benzotriazolyloxy-bis(pyrrolidino)-carbonium hexafluoro- phosphate(BBC)as a coupling reagent with high yield and speed even in low concentration compared with the other usual coupling reagents.展开更多
The linear hexapeptide(His-Gly-Asp) was synthesized on solid support.FM group was used for a carboxyl protection group at Aspl.Peptide was cyclizsed by BOP reagent while still attached to the solid support.BOP proceed...The linear hexapeptide(His-Gly-Asp) was synthesized on solid support.FM group was used for a carboxyl protection group at Aspl.Peptide was cyclizsed by BOP reagent while still attached to the solid support.BOP proceeded more rapidly and gave purer product than DCC/HOBt method.展开更多
The negative ion fast atom bombardment (NIFAB) mass spectra of mono-,di-saccharides and glycosides using phenylboronic acid (PBA) as reagent have been studied.In the ion source,PBA reacts stereospecifically with the m...The negative ion fast atom bombardment (NIFAB) mass spectra of mono-,di-saccharides and glycosides using phenylboronic acid (PBA) as reagent have been studied.In the ion source,PBA reacts stereospecifically with the molecules containing cis-vicinal glycols to form characteristic ions, from which the stereo-isomers of saccharides can be definitely distinguished.Disaccharides and glyco- sides with β-glycosidic linkage seem to be unfavorabale to react with PBA,therefore,by comparison of the abundances of the characteristic ions,the configuration of the glycosidic linkage in these compounds may be inferred.展开更多
文摘The efficiency of TFMSA as a cleaving reagent for peptidyl resin(I-VIII)was evaluated by quantitative ninhydrin test of peptide substitution degree(SD)on resin.Under different conditions,such as variation of C-terminal residue,resin linkage,the temperature and reaction time etc.,TFMSA can be used very conveniently instead of HF in many cases.
基金This work was supported by State Key Laboratory of Bio-organic and Natural products Chemistry.
文摘We report here the synthesis of cyclic peptides using benzotriazolyloxy-bis(pyrrolidino)-carbonium hexafluoro- phosphate(BBC)as a coupling reagent with high yield and speed even in low concentration compared with the other usual coupling reagents.
文摘The linear hexapeptide(His-Gly-Asp) was synthesized on solid support.FM group was used for a carboxyl protection group at Aspl.Peptide was cyclizsed by BOP reagent while still attached to the solid support.BOP proceeded more rapidly and gave purer product than DCC/HOBt method.
文摘The negative ion fast atom bombardment (NIFAB) mass spectra of mono-,di-saccharides and glycosides using phenylboronic acid (PBA) as reagent have been studied.In the ion source,PBA reacts stereospecifically with the molecules containing cis-vicinal glycols to form characteristic ions, from which the stereo-isomers of saccharides can be definitely distinguished.Disaccharides and glyco- sides with β-glycosidic linkage seem to be unfavorabale to react with PBA,therefore,by comparison of the abundances of the characteristic ions,the configuration of the glycosidic linkage in these compounds may be inferred.