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An Effective Prediction Method for Supporting Decision Making in Real Estate Area Selection
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作者 Haoying Jin Song Yang Mingzhi Zhao 《Journal of Computer and Communications》 2024年第7期105-119,共15页
Real estate has been a dominant industry in many countries. One problem for real estate companies is determining the most valuable area before starting a new project. Previous studies on this issue mainly focused on m... Real estate has been a dominant industry in many countries. One problem for real estate companies is determining the most valuable area before starting a new project. Previous studies on this issue mainly focused on market needs and economic prospects, ignoring the impact of natural disasters. We observe that natural disasters are important for real estate area selection because they will introduce considerable losses to real estate enterprises. Following this observation, we first develop a self-defined new indicator named Average Loss Ratio to predict the losses caused by natural disasters in an area. Then, we adopt the existing ARIMA model to predict the Average Loss Ratio of an area. After that, we propose to integrate the TOPSIS model and the Grey Prediction Model to rank the recommendation levels for candidate areas, thereby assisting real estate companies in their decision-making process. We conduct experiments on real datasets to validate our proposal, and the results suggest the effectiveness of the proposed method. 展开更多
关键词 real estate Natural Disaster Decision Making Prediction Model
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4种植物源性成分多重real-time PCR检测方法的建立及其在食用淀粉中的应用 被引量:2
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作者 范维 高晓月 +4 位作者 董雨馨 刘虹宇 李贺楠 赵文涛 郭文萍 《食品科学》 EI CAS CSCD 北大核心 2024年第1期210-216,共7页
建立一种可同时快速检测红薯、木薯、马铃薯、玉米源性成分的多重实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)方法。分别以红薯g3pdh基因、木薯g3pdh基因、马铃薯UGPase基因、玉米zSSIIb基因为靶基因设计... 建立一种可同时快速检测红薯、木薯、马铃薯、玉米源性成分的多重实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)方法。分别以红薯g3pdh基因、木薯g3pdh基因、马铃薯UGPase基因、玉米zSSIIb基因为靶基因设计特异性引物和TaqMan探针,以18S rRNA基因为内参基因,建立多重real-time PCR方法,开展方法学验证,并对不同掺入比例模拟样品和实际淀粉样品进行检测。结果显示,该方法具有高通量、特异性强、灵敏度高等优点。与15种非目标源性均无交叉反应;对目标DNA的检测灵敏度可达到3×10^(-3) ng/μL,且具有良好的线性关系和扩增效率;对淀粉样品的检出限可达0.1%,对50份实际样品进行检测,结果与参比方法一致,说明建立的多重real-time PCR法可用于食用淀粉种类掺假鉴别检测。 展开更多
关键词 多重实时聚合酶链式反应 食用淀粉 木薯 红薯 马铃薯 玉米
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Empirical evidence on the ownership and liquidity of real estate tokens 被引量:1
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作者 Laurens Swinkels 《Financial Innovation》 2023年第1期1246-1274,共29页
To better understand the potential and limitations of the tokenization of real asset mar-kets,empirical studies need to examine this radically new organization of financial mar-kets.In our study,we examine the financi... To better understand the potential and limitations of the tokenization of real asset mar-kets,empirical studies need to examine this radically new organization of financial mar-kets.In our study,we examine the financial and economic consequences of tokenizing 58 residential rental properties in the US,particularly those in Detroit.Tokenization aims at fragmented ownership.We found that the residential properties examined have 254 owners on average.Investors with a greater than USD 5,000 investment in real estate tokens,diversify their real estate ownership across properties within and across the cities.Property ownership changes about once yearly,with more changes for proper-ties on decentralized exchanges.We report that real estate token prices move accord-ing to the house price index;hence,investing in real estate tokens provides economic exposure to residential house prices. 展开更多
关键词 Blockchain Cryptocurrency real estate TOKENIZATION
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Prioritizing real estate enterprises based on credit risk assessment:an integrated multi‑criteria group decision support framework 被引量:1
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作者 Zhen‑Song Chen Jia Zhou +5 位作者 Chen‑Ye Zhu Zhu‑Jun Wang Sheng‑Hua Xiong Rosa M.Rodríguez Luis Martínez Mirosław J.Skibniewski 《Financial Innovation》 2023年第1期2939-2991,共53页
Credit risk assessment involves conducting a fair review and evaluation of an assessed subject’s solvency and creditworthiness.In the context of real estate enterprises,credit risk assessment provides a basis for ban... Credit risk assessment involves conducting a fair review and evaluation of an assessed subject’s solvency and creditworthiness.In the context of real estate enterprises,credit risk assessment provides a basis for banks and other financial institutions to choose suitable investment objects.Additionally,it encourages real estate enterprises to abide by market norms and provide reliable information for the standardized management of the real estate industry.However,Chinese real estate companies are hesitant to disclose their actual operating data due to privacy concerns,making subjective evalu-ation approaches inevitable,occupying important roles in accomplishing Chinese real estate enterprise credit risk assessment tasks.To improve the normative and reliability of credit risk assessment for Chinese real estate enterprises,this study proposes an integrated multi-criteria group decision-making approach.First,a credit risk assessment index for Chinese real estate enterprises is established.Then,the proposed framework combines proportional hesitant fuzzy linguistic term sets and preference ranking organization method for enrichment evaluation II methods.This approach is suitable for processing large amounts of data with high uncertainty,which is often the case in credit risk assessment tasks of Chinese real estate enterprises involving massive subjec-tive evaluation information.Finally,the proposed model is validated through a case study accompanied by sensitivity and comparative analyses to verify its rationality and feasibility.This study contributes to the research on credit assessment for Chinese real estate enterprises and provides a revised paradigm for real estate enterprise credit risk assessment. 展开更多
关键词 real estate enterprise Credit risk assessment PROMETHEE II Best–worst method Proportional hesitant fuzzy linguistic term sets
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Research on the intelligent supervision and operation platform of railway real estate 被引量:2
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作者 Xiangru Lv Hui Li Beisheng Liu 《High-Speed Railway》 2023年第2期147-152,共6页
Railway real estate is the fundamental element of railway transportation production and operation.Effective management and rational utilization of railway real estate is essential for railway asset operation.Based on ... Railway real estate is the fundamental element of railway transportation production and operation.Effective management and rational utilization of railway real estate is essential for railway asset operation.Based on the investigation of the requirements of railway real estate management and operation,combined with Beidou positioning,GIS(Geographic Information System),multi-source data fusion and other cutting-edge technologies,this paper puts forward the multi-dimensional dynamic statistical method of real estate information,the identification method of railway land occupation and the comprehensive evaluation method of real estate development and utilization potential,and build the railway real estate supervision and operation platform,design the function of the platform,so as to provide intelligent solutions for the railway real estate operation. 展开更多
关键词 RAILWAY real estate supervision GIS Beidou positioning Multi-sourcedata fusion
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Architecting the Metaverse: Blockchain and the Financial and Legal Regulatory Challenges of Virtual Real Estate 被引量:1
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作者 James Hutson Gaurango Banerjee +2 位作者 Naresh Kshetri Kurt Odenwald Jeremiah Ratican 《Journal of Intelligent Learning Systems and Applications》 2023年第1期1-23,共23页
There has been disagreement over the value of purchasing space in the metaverse, but many businesses including Nike, The Wendy’s Company, and McDonald’s have jumped in headfirst. While the metaverse land rush has be... There has been disagreement over the value of purchasing space in the metaverse, but many businesses including Nike, The Wendy’s Company, and McDonald’s have jumped in headfirst. While the metaverse land rush has been called an “illusion” given underdeveloped infrastructure, including inadequate software and servers, and the potential opportunities for economic and legal abuse, the “real estate of the future” shows no signs of slowing. While the current virtual space of the metaverse is worth $6.30 billion, that is expected to grow to $84.09 billion by the end of 2028. But the long-term legal and regulatory considerations of capitalizing on the investment, as well as the manner in which blockchain technology can secure users’ data and digital assets, has yet to be properly investigated. With the metaverse still in a conceptual phase, building a new 3D social environment capable of digital transactions will represent most of the initial investment in time in human capital. Digital twin technologies, already well-established in industry, will be ported to support the need to architect and furnish the new digital world. The return on and viability of investing in the “real estate of the future” raises questions fundamental to the success or failure of the enterprise. As such this paper proposes a novel framing of the issue and looks at the intersection where finance, technology, and law are converging to prevent another Dot-com bubble of the late 1990s in metaverse-based virtual real estate transactions. Furthermore, the paper will argue that these domains are technologically feasible, but the main challenges for commercial users remain in the legal and regulatory arenas. As has been the case with the emergence of online commerce, a legal assessment of the metaverse indicates that courts will look to traditional and established legal principles when addressing issues until the enactment of federal and/or state statutes and accompanying regulations. Lastly, whereas traditional regulation of real estate would involve property law, the current legal framing of ownership of metaverse assets is governed by contract law. 展开更多
关键词 Blockchain Digital real estate Digital Retail Digital Twin Digital Content FINANCE Metaverse
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血-迷路屏障通透性与突聋发病机制及预后的关系:在3D real IR的发现
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作者 张呈辉 任芳芳 +2 位作者 王伟韬 郝芳 冀少杰 《听力学及言语疾病杂志》 CAS CSCD 北大核心 2024年第3期236-240,共5页
目的分析突聋患者的内耳钆造影MRI三维真实重建反转恢复(three dimensional real inversion recovery,3D real IR)成像上的表现,探讨血-迷路屏障的通透性与突聋发病机制及其预后的关系。方法对41例单侧突聋患者行内耳钆造影MRI,测量患... 目的分析突聋患者的内耳钆造影MRI三维真实重建反转恢复(three dimensional real inversion recovery,3D real IR)成像上的表现,探讨血-迷路屏障的通透性与突聋发病机制及其预后的关系。方法对41例单侧突聋患者行内耳钆造影MRI,测量患耳和健耳的耳蜗信号强度,并测量延髓信号强度,分别计算出耳蜗/延髓比值(cochlear/medulla ratio,CM ratio),以CM比值作为血-迷路屏障通透性的标志物,分析突聋患者患耳、健耳CM比值的不对称程度与疗效之间的关系。结果41例患者中,33例(80.48%)患耳的CM比值高于健耳,差异有统计学意义(P<0.05);患耳CM比值为健耳的1.5倍以下者18例,治疗有效率为77.78%(14/18);患侧CM比值不高于健侧者8例,治疗有效率为100%;达到健耳的1.5倍至1.75倍之间者7例,治疗有效率为100%(7/7);达到健耳的1.75倍至2.0倍之间者2例,治疗有效率为50%(1/2);达到健耳的2.0倍以上者14例,治疗有效率为14.28%(12/14);差异有统计学意义(P<0.05)。结论内耳3D Real IR可显示突聋患者血-迷路屏障通透性的改变,80.48%的突聋患者患侧耳蜗出现高信号,患耳CM比值达健耳的1.75倍以上者多数预后不良。 展开更多
关键词 突聋 血-迷路屏障通透性 内耳三维真实重建反转恢复成像 耳蜗/延髓比值 预后
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A Concept about Guide on Hazards for the Real Estate Business-An Example from Bulgaria
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作者 Boyko Ranguelov Rumiana Glavcheva 《Journal of Civil Engineering and Architecture》 2023年第2期101-107,共7页
The paper is a part of wider investigation related to the application of basic sciences to the sustainable development of society.It deals with the presentation of knowledge to the real estate(fast growing business)ab... The paper is a part of wider investigation related to the application of basic sciences to the sustainable development of society.It deals with the presentation of knowledge to the real estate(fast growing business)about the local natural and man-made hazards in the near vicinity of any structure(inhabitant,recreation area,land,etc.)and their influence to the social safety and human comfort of life.The structure,necessary information and knowledge base for visible or hidden hazards about a guide for the real estate business is included.An example of application the knowledge about seismic hazard and related phenomena simplified to be more available to the non-specialists(such as real estate agents and companies)targeted to Sofia(the largest urban center and capital of Bulgaria)can attract and improve the learning abilities of the real estate for the society’s sake. 展开更多
关键词 real estate local environment hazards knowledge guide
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A Review of Domestic and Foreign Research on Real Estate Financial Management
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作者 Xiuzhi Yuan 《Proceedings of Business and Economic Studies》 2023年第2期9-19,共11页
With the tightening of house purchase policies in recent years, the introduction of financial and land restriction policies, as well as the impact of the COVID-19 pandemic, real estate bombshells are common. Real esta... With the tightening of house purchase policies in recent years, the introduction of financial and land restriction policies, as well as the impact of the COVID-19 pandemic, real estate bombshells are common. Real estate is in the critical stage of transformation and upgrading, and industrial development is facing dire challenges and pressure for survival. As an important part of enterprise management, financial management is directly related to the success or failure of enterprise development. Facing the financial risks emerging in the financial management of the real estate industry at this stage, this review begins from financial management personnel training, investment calculation management, budget management, and asset management in the effort of optimizing the real estate financial management operating mechanism under the new situation and ensuring the healthy and stable development of real estate enterprises, while maintaining core competitiveness. 展开更多
关键词 real estate Financial management Investment calculation BUDGET
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Examining the Impact Factors and Strategies to Mitigate Economic Fluctuation in the Real Estate Sector
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作者 Yadong Xiao 《Proceedings of Business and Economic Studies》 2023年第5期54-58,共5页
With the continuous growth of the economy and the rapid pace of urbanization,the real estate sector has assumed an increasingly substantial role within the national economy.Ensuring the stable and sustainable developm... With the continuous growth of the economy and the rapid pace of urbanization,the real estate sector has assumed an increasingly substantial role within the national economy.Ensuring the stable and sustainable development of the real estate sector to maximize its societal and economic contributions has become a pressing issue.China,presently engaged in an extensive urbanization drive,places real estate development and sales as pivotal components in the broader real estate industry,significantly impacting the overall functionality of the real estate market.Real estate,being a comprehensive system encompassing land,housing,infrastructure,and other key elements,exerts a profound influence on people’s basic needs such as shelter,sustenance,and daily living.Its evolution directly shapes the trajectory of the national economy and social stability.Therefore,to foster the consistent and robust growth of China’s real estate economy,it is necessary to implement effective measures that entail a systematic and thorough analysis of the factors responsible for the fluctuations in China’s real estate economy. 展开更多
关键词 real estate Economic fluctuations Influencing factors COUNTERMEASURES
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Real-time Rescue Target Detection Based on UAV Imagery for Flood Emergency Response 被引量:1
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作者 ZHAO Bofei SUI Haigang +2 位作者 ZHU Yihao LIU Chang WANG Wentao 《Journal of Geodesy and Geoinformation Science》 CSCD 2024年第1期74-89,共16页
Timely acquisition of rescue target information is critical for emergency response after a flood disaster.Unmanned Aerial Vehicles(UAVs)equipped with remote sensing capabilities offer distinct advantages,including hig... Timely acquisition of rescue target information is critical for emergency response after a flood disaster.Unmanned Aerial Vehicles(UAVs)equipped with remote sensing capabilities offer distinct advantages,including high-resolution imagery and exceptional mobility,making them well suited for monitoring flood extent and identifying rescue targets during floods.However,there are some challenges in interpreting rescue information in real time from flood images captured by UAVs,such as the complexity of the scenarios of UAV images,the lack of flood rescue target detection datasets and the limited real-time processing capabilities of the airborne on-board platform.Thus,we propose a real-time rescue target detection method for UAVs that is capable of efficiently delineating flood extent and identifying rescue targets(i.e.,pedestrians and vehicles trapped by floods).The proposed method achieves real-time rescue information extraction for UAV platforms by lightweight processing and fusion of flood extent extraction model and target detection model.The flood inundation range is extracted by the proposed method in real time and detects targets such as people and vehicles to be rescued based on this layer.Our experimental results demonstrate that the Intersection over Union(IoU)for flood water extraction reaches an impressive 80%,and the IoU for real-time flood water extraction stands at a commendable 76.4%.The information on flood stricken targets extracted by this method in real time can be used for flood emergency rescue. 展开更多
关键词 UAV flood extraction target rescue detection real time
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Real eigenvalues determined by recursion of eigenstates
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作者 刘通 王友国 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第3期196-200,共5页
Quantum physics is primarily concerned with real eigenvalues,stemming from the unitarity of time evolutions.With the introduction of PT symmetry,a widely accepted consensus is that,even if the Hamiltonian of the syste... Quantum physics is primarily concerned with real eigenvalues,stemming from the unitarity of time evolutions.With the introduction of PT symmetry,a widely accepted consensus is that,even if the Hamiltonian of the system is not Hermitian,the eigenvalues can still be purely real under specific symmetry.Hence,great enthusiasm has been devoted to exploring the eigenvalue problem of non-Hermitian systems.In this work,from a distinct perspective,we demonstrate that real eigenvalues can also emerge under the appropriate recursive condition of eigenstates.Consequently,our findings provide another path to extract the real energy spectrum of non-Hermitian systems,which guarantees the conservation of probability and stimulates future experimental observations. 展开更多
关键词 real eigenvalues NON-HERMITIAN QUASIPERIODIC
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基于Real ESRGAN的视频修复系统研究
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作者 黄杰 夏远洋 +3 位作者 杨晓杰 王思洁 田佩 刘涛 《科技创新与应用》 2024年第15期46-49,54,共5页
图像修复和视频修复是计算机视觉的一项重要任务,其中图像修复又是视频修复的基础。为此,如何有效提升图像质量是实现视频质量提升的关键。传统的图像修复算法主要以样本信息为基础,通过对样本内容的扩撒来实现对破损区域的修复;由于这... 图像修复和视频修复是计算机视觉的一项重要任务,其中图像修复又是视频修复的基础。为此,如何有效提升图像质量是实现视频质量提升的关键。传统的图像修复算法主要以样本信息为基础,通过对样本内容的扩撒来实现对破损区域的修复;由于这种方式对于图像样本有一定要求,从而制约传统图像修复技术的发展。为此,以生成新图像内容为基础的神经网络如GAN的出现,为图像修复技术转向深度学习提供方向。该课题主要以Real ESRGAN网络的图像修复技术为基础,通过对音频视频数据的隔离处理以及相同帧数据的优化和标记,构建视频修复处理流程。通过对随机视频样本的测试,并通过对单帧图片质量和视频数据流畅性与协调性的评估,该视频处理方法表现出较好的系统性能。 展开更多
关键词 图像分割 神经网络 视频修复 real ESRGAN 图像修复
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A Stochastic Model to Assess the Epidemiological Impact of Vaccine Booster Doses on COVID-19 and Viral Hepatitis B Co-Dynamics with Real Data
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作者 Andrew Omame Mujahid Abbas Dumitru Baleanu 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第3期2973-3012,共40页
A patient co-infected with COVID-19 and viral hepatitis B can be atmore risk of severe complications than the one infected with a single infection.This study develops a comprehensive stochastic model to assess the epi... A patient co-infected with COVID-19 and viral hepatitis B can be atmore risk of severe complications than the one infected with a single infection.This study develops a comprehensive stochastic model to assess the epidemiological impact of vaccine booster doses on the co-dynamics of viral hepatitis B and COVID-19.The model is fitted to real COVID-19 data from Pakistan.The proposed model incorporates logistic growth and saturated incidence functions.Rigorous analyses using the tools of stochastic calculus,are performed to study appropriate conditions for the existence of unique global solutions,stationary distribution in the sense of ergodicity and disease extinction.The stochastic threshold estimated from the data fitting is given by:R_(0)^(S)=3.0651.Numerical assessments are implemented to illustrate the impact of double-dose vaccination and saturated incidence functions on the dynamics of both diseases.The effects of stochastic white noise intensities are also highlighted. 展开更多
关键词 Viral hepatitis B COVID-19 stochastic model EXTINCTION ERGODICITY real data
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A Comparative Study between Landmark Based and Real Time Ultrasound Guided Sub Arachnoid Block
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作者 Kunal Tewari Om Bahadur Thapa +4 位作者 Deepak Mishra Manjot Multani Jyotsna Sharma Akash Ray Mohapatra Sandhya Khwaunju 《Open Journal of Anesthesiology》 2024年第4期118-125,共8页
Background: Sub arachnoid block (SAB) performed by traditional landmark palpation technique can be inaccurate. This problem is exacerbated by altered patient anatomy due to obesity and age-related changes. A pre-proce... Background: Sub arachnoid block (SAB) performed by traditional landmark palpation technique can be inaccurate. This problem is exacerbated by altered patient anatomy due to obesity and age-related changes. A pre-procedural ultrasound scan of the lumbar spine has been shown to be of benefit in guiding lumbar epidural insertion in obstetric patients. Information on the use of real-time ultrasound (RUS) guided SAB, to date, been limited. This study compared RUS guided SAB to traditional landmark guided technique in patients undergoing spinal anesthesia for different surgical procedures. Methods: This was a prospective, single center, comparative observational study conducted in the department of anesthesiology at our center. 560 patients who underwent spinal anesthesia either by landmark based technique or real-time ultrasound-guided methods. The primary outcome was the first attempt success rate of dural puncture when employing the two methods. Results: Baseline characteristics were similar in the two study groups. The first attempt success rate of dural puncture in landmark guided group was 64.3% compared to 72.6% in the ultrasound guided group. This difference was not statistically significant. The procedure performance time was significantly shorter with landmark palpation compared to use of real-time ultrasound guided method. Conclusion: Use of RUS-guided technique does not significantly improve the first attempt success rate of SAB dural puncture during spinal anesthesia compared to the traditional landmark-guided technique. 展开更多
关键词 Sub Arachnoid Block (SAB) real Time Ultrasound (RUS)
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马乳酒样乳杆菌马乳酒样亚种real-time PCR检测方法的建立与应用
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作者 吕厚姣 李欣媛 +3 位作者 白小佳 贾龙刚 耿伟涛 王艳萍 《食品科学》 EI CAS CSCD 北大核心 2024年第9期102-108,共7页
本研究建立了一种特异性实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)检测方法,根据模式菌株马乳酒样乳杆菌马乳酒样亚种ZW3的16S rDNA序列和全基因组序列设计筛选特异性引物,采用SYBR Green I荧光染料建立r... 本研究建立了一种特异性实时聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)检测方法,根据模式菌株马乳酒样乳杆菌马乳酒样亚种ZW3的16S rDNA序列和全基因组序列设计筛选特异性引物,采用SYBR Green I荧光染料建立real-time PCR方法,并对方法的特异性、灵敏度、重复性和混合体系等进行检测。结果表明,本研究所建立的方法特异性强、灵敏度高、重复性好,建立real-time PCR的标准曲线,其决定系数R2为0.965,具有良好的线性关系,且在马乳酒样乳杆菌马乳酒样亚种及混合体系中可以特异性检出。综上,本研究建立的real-time PCR法可以快速、准确地检测马乳酒样乳杆菌马乳酒样亚种,为马乳酒样乳杆菌的特异性定性定量检测提供了一种新的方法。 展开更多
关键词 马乳酒样乳杆菌马乳酒样亚种 实时聚合酶链式反应 特异性引物
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基于病菌孢子捕捉和real-time PCR技术的田间空气中小麦白粉病菌孢子动态监测及病情估计模型研究
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作者 王奥霖 商昭月 +8 位作者 张美惠 王贵 胡小平 徐飞 孙振宇 曹世勤 刘伟 范洁茹 周益林 《植物保护》 CAS CSCD 北大核心 2024年第2期49-56,72,共9页
利用Burkard定容式孢子捕捉器结合real-time PCR定量技术,分别对种植高抗、中感和高感白粉病小麦品种的田间空气中白粉病菌分生孢子浓度进行监测,结果表明,real-time PCR定量与传统的显微观察计数两种方法测得的孢子浓度呈显著正相关(P... 利用Burkard定容式孢子捕捉器结合real-time PCR定量技术,分别对种植高抗、中感和高感白粉病小麦品种的田间空气中白粉病菌分生孢子浓度进行监测,结果表明,real-time PCR定量与传统的显微观察计数两种方法测得的孢子浓度呈显著正相关(P≤0.01),且两种病菌孢子计数方法在同一抗性品种上监测到的孢子浓度动态相近。此外,两种方法测得的孢子浓度与各气象因子的相关性分析结果一致,空气中的白粉病菌孢子浓度主要与空气相对湿度显著正相关。在此基础上,利用两种方法测定的田间空气中白粉病菌孢子浓度分别建立了基于累积孢子浓度的田间病情估计模型。分析发现,基于两种孢子浓度测定方法建立的病情估计模型间无显著性差异,表明real-time PCR定量技术测定的孢子浓度在构建白粉病病情估计模型上具有一定可行性。该结果为real-time PCR定量技术与病菌孢子捕捉技术相结合用于小麦白粉病的监测和预测提供理论依据。 展开更多
关键词 小麦白粉病 病菌孢子捕捉 实时荧光定量PCR 病原菌监测 病情估计模型
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基于Real-time PCR法检测乳粉中牛源性成分定量研究
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作者 陈晨 史国华 +5 位作者 陈勃旭 张瑞 王玉欣 贾文珅 陈佳 周巍 《粮油食品科技》 CAS CSCD 北大核心 2024年第2期159-164,共6页
基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛... 基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛源性成分的相对定量检测。结果显示,该方法的最低检测限为0.00001 mg/mL,回收率为91.11%~119.2%,组间变异系数≤0.58%、组内变异系数≤1.44%。说明该方法在特异性与稳定性上适用于乳粉中牛源性成分及含量的掺假检测。 展开更多
关键词 牛乳粉 马乳粉 real-time PCR 掺假检测
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一种基于real-time PCR技术的TTV检测方法的建立及应用
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作者 贾毅博 王高玉 +4 位作者 邓宛心 林彩云 杨华 陈运春 尹飞飞 《海南医学院学报》 CAS 北大核心 2024年第7期489-497,共9页
目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域... 目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域的real-time PCR检测方法,并与文献报道应用较为广泛的PCR检测方法进行了对比。结果:本研究建立的方法在1×10^(7)~1×10^(1) copies/μL标准品浓度范围内具有良好的线性关系,相关系数为1.000,斜率为-3.446,检测下限为1×10^(1) copies/μL。重复性试验结果显示,组内变异系数为7.22%,表明本方法重复性、稳定性较强。针对30份临床样本,使用本研究建立的real-time PCR检测方法及目前被多个研究所使用的4套引物进行对比。结果表明,本研究所建立的方法灵敏度显著高于文献中报道的4种方法(P<0.01);Sanger测序结果表明,本方法检测出的30份阳性样本均为TTV,检测特异性为100%。结论:本研究采用基于TaqMan探针的real-time PCR检测方法,检测灵敏性高、覆盖基因型范围广,尤其对于TTV病毒载量较低的情况下能够进行定量检测,对于TTV病毒的致病性及作为免疫标志物的应用提供重要的技术支持。 展开更多
关键词 Torque teno virus 基因组扩增测序 real-time PCR检测
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Research on Value Evaluation Method of Investment Project Based on Fuzzy Composite Real Options
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作者 Huanyu Li 《Economics World》 2024年第1期24-34,共11页
Venture capital investments are characterized by high input,high yield,and high risk.Due to the complexity of the market environment,stage-by-stage investment is becoming increasingly important.Traditional evaluation ... Venture capital investments are characterized by high input,high yield,and high risk.Due to the complexity of the market environment,stage-by-stage investment is becoming increasingly important.Traditional evaluation methods like comparison,proportion,maturity,internal rate of return,scenario analysis,decision trees,and net present value cannot fully consider the uncertainty and stage characteristics of the project.The fuzzy real options method addresses this by combining real option theory,fuzzy number theory,and composite option theory to provide a more accurate and objective evaluation of Public-Private Partnership(PPP)projects.It effectively considers the interaction of options and the ambiguity of project parameters,making it a valuable tool for project evaluation in the context of venture capital investment. 展开更多
关键词 real option fuzzy method Geske composite option
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