基于病菌孢子捕捉和real-time PCR技术的田间空气中小麦白粉病菌孢子动态监测及病情估计模型研究

利用Burkard定容式孢子捕捉器结合real-time PCR定量技术,分别对种植高抗、中感和高感白粉病小麦品种的田间空气中白粉病菌分生孢子浓度进行监测,结果表明,real-time PCR定量与传统的显微观察计数两种方法测得的孢子浓度呈显著正相关(P≤0.01),且两种病菌孢子计数方法在同一抗性品种上监测到的孢子浓度动态相近。此外,两种方法测得的孢子浓度与各气象因子的相关性分析结果一致,空气中的白粉病菌孢子浓度主要与空气相对湿度显著正相关。在此基础上,利用两种方法测定的田间空气中白粉病菌孢子浓度分别建立了基于累积孢子浓度的田间病情估计模型。分析发现,基于两种孢子浓度测定方法建立的病情估计模型间无显著性差异,表明real-time PCR定量技术测定的孢子浓度在构建白粉病病情估计模型上具有一定可行性。该结果为real-time PCR定量技术与病菌孢子捕捉技术相结合用于小麦白粉病的监测和预测提供理论依据。

基于Simulink Real-Time的汽车空调半实物仿真试验系统研发

针对多样化汽车空调电子零部件的高效、低成本总成级测试需求,研究基于一汽大众速腾1.6 L车型原型,设计了基于Simulink Real-Time的汽车空调半实物仿真试验系统。该系统集成了真实的汽车空调总成、制冷循环以及包含宿主机和目标机的双机实时仿真系统,用于开发和运行台架监控和空调控制模型,为汽车空调电子零部件提供半实物仿真测试环境。通过系统集成及功能验证,结果表明,汽车空调半实物仿真试验系统能够实现汽车空调基本控制功能,同时在不同工况下实现乘员舱温度控制的稳定性,稳态误差控制在±1℃以内。此外,该系统还具备灵活调整空调运行工况和状态的能力,为汽车空调电子零部件提供了高效、低成本的半实物仿真测试环境。

Onboard real time modeling of aircraft engines with Hammerstein-Wiener representation

An identification-based approach for aircraft engine modeling using the nonlinear HammersteinWiener representation was proposed.Hammerstein-Wiener modeling for both limited flight envelope and extended flight envelope was investigated.Simulation shows that the resulting model can be valid over 10%variation of rotational speed of the engine,compared with those linear models that are only valid over 3%—5%change of rotational speed.It is further demonstrated that the proposed method can be utilized over large envelope up to 20% variation of rotational speed of the engine.The fundamental idea is to use nonlinear models to extend the feasible/valid region rather than those linear models.This may consequently simplify the switching logic in the onboard digital control units.This is often overlooked in aircraft engine control community,but has been emphasized in the research.

On modeling approach for embedded real-time software simulation testing

Modeling technology has been introduced into software testing field. However, how to carry through the testing modeling effectively is still a difficulty. Based on combination of simulation modeling technology and embedded real-time software testing method, the process of simulation testing modeling is studied first. And then, the supporting environment of simulation testing modeling is put forward. Furthermore, an approach of embedded real-time software simulation testing modeling including modeling of cross-linked equipments of system under testing （SUT）, test case, testing scheduling, and testing system service is brought forward. Finally, the formalized description and execution system of testing models are given, with which we can realize real-time, closed loop, mad automated system testing for embedded real-time software.

Experimental Model and Analytic Solution for Real-time Observation of Vehicle's Additional Steer Angle

The current research of real-time observation for vehicle roll steer angle and compliance steer angle（both of them comprehensively referred as the additional steer angle in this paper） mainly employs the linear vehicle dynamic model, in which only the lateral acceleration of vehicle body is considered. The observation accuracy resorting to this method cannot meet the requirements of vehicle real-time stability control, especially under extreme driving conditions. The paper explores the solution resorting to experimental method. Firstly, a multi-body dynamic model of a passenger car is built based on the ADAMS/Car software, whose dynamic accuracy is verified by the same vehicle＇s roadway test data of steady static circular test. Based on this simulation platform, several influencing factors of additional steer angle under different driving conditions are quantitatively analyzed. Then ε-SVR algorithm is employed to build the additional steer angle prediction model, whose input vectors mainly include the sensor information of standard electronic stability control system（ESC）. The method of typical slalom tests and FMVSS 126 tests are adopted to make simulation, train model and test model＇s generalization performance. The test result shows that the influence of lateral acceleration on additional steer angle is maximal （the magnitude up to 1°）, followed by the longitudinal acceleration-deceleration and the road wave amplitude （the magnitude up to 0.3°）. Moreover, both the prediction accuracy and the calculation real-time of the model can meet the control requirements of ESC This research expands the accurate observation methods of the additional steer angle under extreme driving conditions.

A Novel Approach to Study Regional Ionospheric Variations Using a Real-Time TEC Model

Since IGY (International Geophysical Year), through coordinated global observations, ionospheric research has been carried out by many countries. This effort primarily helped in the design and operation of HF radio wave communication systems. The Indian region covers a highly variable part of the equatorial electrojet and EIA (Equatorial Ionisation Anomaly) phenomena making its predictability difficult. With the advent of satellite communication and navigation, the need for accurate ionospheric TEC (Total Electron Content) models at global and regional scales has been stressed. The GAGAN (GPS Aided Geo Augmented Navigation) project jointly undertaken by the Indian Space Research Organisation (ISRO) and the Airport Authority of India (AAI) aims at effectively utilising the Global Navigational Satellite System (GNSS) to determine position coordinates accurately for aircraft precision landing applications. For this purpose the range errors are estimated by using a ground network of TEC stations spread over Indian region. The near simultaneous data collected from these dual frequency GPS stations can be used to generate the geo-referenced TEC values for various applications. The author has developed necessary algorithm and associated computer programmes for a real-time vertical TEC (VTEC) model based on TEC data collected from the GAGAN ground based network stations. The model has been tested and sample results presented here show that it adequately provides for the latitudinal resolution of 1° for the entire longitude span and also for two longitude blocks (73 - 83 & 83 - 93°E) separately. Cubic spline and bilinear interpolation techniques are used for filling up temporal and spatial data gaps. The model provides tabulated output of hourly average VTEC data with latitude for ready use, as well as graphical displays of VTEC maps and contours for monitoring purpose. The real-time model and its extensions are also being used for detailed scientific studies;examples of these show small day to day variability of VTEC without any change in solar activity and indication of the change in the shape of the VTEC diurnal curve with season. The present model will be used for further studies to derive the monthly average variation of the diurnal pattern and the relationship between VTEC peak amplitudes with changes in solar activity. The new information generated can be fed back to improve the real-time model so that eventually the dependence of such models on ground based network stations data can be minimised.

THE DLSTRIBOTED MCU MODEL FOR SYNCHRONOUS REAL-TIME TELETEACHING

Aiming at issues on multimedia communication in synchronous real-time teleteaching (SRT) systems over IP network, a 4-tuple structural mode of multimedia communication is proposed in the paper, and an SRT-oriented distributed MCU model is built according to the mode. Moreover, the mechanism of multicast communication across subnets is discussed. The distributed MCU model has been applied successfully in our interactive synchronous real-time telesteaching system RealClass and has shown good extendibility in operation.

Formal Modeling and Analysis of AADL Threads in Real Time Maude

This paper presents, without altering the AADL meta-model, a formal description of static and behavioral aspects of the AADL thread component. This active and concurrent applicative component of AADL poses many challenges to its formalization and analysis including instantaneous and/or delayed communications, concurrent tasks and time-dependent features, and the need to analyze correctness. This formalization, based on real-time object-oriented theories, allows not only a precise description of the semantics of threads composition with respect to their timing requirements but also makes possible the formal verification of behavioral properties.

Model-based framework for multi-axial real-time hybrid simulation testing

Real-time hybrid simulation is an efficient and cost-effective dynamic testing technique for performance evaluation of structural systems subjected to earthquake loading with rate-dependent behavior. A loading assembly with multiple actuators is required to impose realistic boundary conditions on physical specimens. However, such a testing system is expected to exhibit significant dynamic coupling of the actuators and suffer from time lags that are associated with the dynamics of the servo-hydraulic system, as well as control-structure interaction （CSI）. One approach to reducing experimental errors considers a multi-input, multi-output （MIMO） controller design, yielding accurate reference tracking and noise rejection. In this paper, a framework for multi-axial real-time hybrid simulation （maRTHS） testing is presented. The methodology employs a real-time feedback-feedforward controller for multiple actuators commanded in Cartesian coordinates. Kinematic transformations between actuator space and Cartesian space are derived for all six-degrees-of- freedom of the moving platform. Then, a frequency domain identification technique is used to develop an accurate MIMO transfer function of the system. Further, a Cartesian-domain model-based feedforward-feedback controller is implemented for time lag compensation and to increase the robustness of the reference tracking for given model uncertainty. The framework is implemented using the 1/5th-scale Load and Boundary Condition Box （LBCB） located at the University of Illinois at Urbana- Champaign. To demonstrate the efficacy of the proposed methodology, a single-story frame subjected to earthquake loading is tested. One of the columns in the fraane is represented physically in the laboratory as a cantilevered steel column. For real- time execution, the numerical substructure, kinematic transformations, and controllers are implemented on a digital signal processor. Results show excellent performance of the maRTHS framework when six-degrees-of-freedom are controUed at the interface between substructures.

MODEL-BASED DEVELOPMENT OF REAL-TIME SOFTWARE SYSTEM FOR ELECTRONIC UNIT PUMP SYSTEM

A real-time operating system （RTOS）, also named OS, is designed based on the hardware platform of MC68376, and is implemented in the electronic control system for unit pump in diesel engine. A parallel and time-based task division method is introduced and the multi-task software architecture is built in the software system for electronic unit pump （EUP） system. The V-model software development process is used to control algorithm of each task. The simulation results of the hardware-in-the-loop simulation system （HILSS） and the engine experimental results show that the OS is an efficient real-time kernel, and can meet the real-time demands of EUP system; The built multi-task software system is real-time, determinate and reliable. V-model development is a good development process of control algorithms for EUP system, the control precision of control system can be ensured, and the development cycle and cost are also decreased.

运用Real-time quantification PCR方法建立副溶血性弧菌在即食虾中的生长预测模型

运用Real-time quantification PCR(real-time qPCR)方法建立副溶血性弧菌在即食虾中生长预测模型。首先构建质粒标准品,梯度稀释后建立标准曲线,然后用Real-time qPCR方法检测虾中副溶血性弧菌的数量,最后建立37℃下即食虾中副溶血性弧菌生长预测模型,并与传统涂布计数方法进行比较。结果表明,Real-time qPCR方法和传统计数方法均可建立Gmopertz模型、Logistic模型和Richards模型,模型拟合的相关系数R2均在0.9以上。基于Real-timeqPCR方法省时省力、特异性好等优点,用Real-time qPCR方法建立微生物预测模型是未来预测微生物学领域的一种发展趋势。

Specifying Requirements of Real-Time System with Rules and Templates

This paper presents a model specifying requirements of real-time systems. Different from existing researches, this model mainly uses rules and templates to represent hierarchical FSMs (Finite State Machine). In this model, one rule corresponds to one state transition of FSM and one template corresponds to one FSM. Rules and information with respect to a FSM can be written in a template. So templates include not only state diagrams, but also information that can not be described by FSM, such as performance requirements. The specification using this model consists of a collection of templates and it is easy for users to understand and to review. After introduced the related researches and principles of the model, this paper specifies requirements of a real-time system with this model, and discusses characters of this model in the end.

Real-Time Rendering of Large-Scale Ocean Environments

Simulation and rendering of large-scale natural environments, especially the ocean, has always been one of the hot issues in computer graphics, which can provide realism for various applications such as computer game, movie and military usage. Simulation of ocean environment is often lack of realism for real-time application due to its complexity of dynamic waves. In this paper, a method based on FFT Wave model is proposed to solve this problem, which can also simulate the ocean optic property with atmosphere scattering. Furthermore, our method has a lot of advantages including global ocean dataset support, real-time, dynamic reflection of ocean, the foam on the wave, smooth transition from deep ocean to seacoast, etc. The experimental results demonstrate the realism and effectiveness of our approach.

Real-time 3-D space numerical shake prediction for earthquake early warning

In earthquake early warning systems, real-time shake prediction through wave propagation simulation is a promising approach. Compared with traditional methods, it does not suffer from the inaccurate estimation of source parameters. For computation efficiency, wave direction is assumed to propagate on the 2-D surface of the earth in these methods. In fact, since the seismic wave propagates in the 3-D sphere of the earth, the 2-D space modeling of wave direction results in inaccurate wave estimation. In this paper, we propose a 3-D space numerical shake pre- diction method, which simulates the wave propagation in 3-D space using radiative transfer theory, and incorporate data assimilation technique to estimate the distribution of wave energy. 2011 Tohoku earthquake is studied as an example to show the validity of the proposed model. 2-D space model and 3-D space model are compared in this article, and the prediction results show that numerical shake prediction based on 3-D space model can estimate the real-time ground motion precisely, and overprediction is alleviated when using 3-D space model.

We’ve Got the Power: A Framework for Real-Time Network Power Monitoring

The energy consumption of the information and communication technology sector has become a significant portion of the total global energy consumption, warranting research efforts to attempt to reduce it. The pre-requisite for effectual energy management is the availability of the current power consumption values from network devices. Previous works have attempted to estimate and model the consumption values or have measured it using intrusive approaches such as using an in-line power meter. Recent trends suggest that information models are being increasingly used in all aspects of network management. This paper presents a framework developed for enabling the collection of real-time power consumption information from the next generation of networking hardware non-intrusively by employing information models. The experiment results indicate that it is feasible to gather power consumption data using standardized IETF information models, or non-standard customized information models, or through abstracting and exposing the information in a uniform format when no support for the required information models exists. Functional validation of the proposed framework is performed and the results from this research could be leveraged to make energy-efficient network management decisions.

Improved Radio Network Dimensioning for Real-Time Polling Service on IEEE 802.16 Wireless Networks with QoS Consideration

Recently, applications of real-time polling service (rtPS) in IEEE 802.16 wireless networks have gained considerable popularity. These applications generate large amounts of real time traffic in the network and thus maintaining the quality of service (QoS) such as packet delay requirement in rtPS dominant networks is critical. Existing dimensioning methodology does not consider QoS parameters of rtPS in network dimensioning. Moreover, exhaustive and time-consuming simulations are required to evaluate the performance and QoS of rtPS. To overcome this problem, we propose an improved radio network dimensioning framework which considers QoS parameters of rtPS in network dimensioning. In this framework, an analytical model is developed to evaluate the capacity and performance of rtPS in IEEE 802.16 wireless networks. The proposed framework provides a fast and accurate means of finding the trade-off between system load and packet delay, thus providing network operators with an analytical tool that jointly considers coverage, capacity and QoS requirements for obtaining the minimum number of sites required. The accuracy of the proposed model is validated through simulations.

An Extension of Contract Net Protocol With Real Time Constraints

The contract net protocol has developed to specify problem solving communication for nodes in a distributed problem solving. Task distribution is affected by a negotiation process,a discussion carried on between nodes with tasks to he executed and nodes that may be able to execute those tasks. In contract net protocol,once negotiation successes,tbe task execution is assumed to success. However,in real world,even though a task is awarded to successfully bidding nodes,it may be delayed. Such delay may badly propagate in whole system. Here,we introduce real-time constraints into contract net protocol to manage task execution for avoiding the, task's delay,or even though being delayed,the railure cannot propagate to whole system. In this paper,we first present a real-time contract net protocol which is an extension of contract net protocol with real-time constraints for distributed computing. Our proposition extends the basic negotiation protocol to negotiation and controlling execution or task. The controlling process is based on task deadline time,we also present an extension of the internode language of contract net protocol specification with real-time constraints.