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Real-Time Monitoring Method for Cow Rumination Behavior Based on Edge Computing and Improved MobileNet v3
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作者 ZHANG Yu LI Xiangting +4 位作者 SUN Yalin XUE Aidi ZHANG Yi JIANG Hailong SHEN Weizheng 《智慧农业(中英文)》 CSCD 2024年第4期29-41,共13页
[Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been propo... [Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been proposed for monitoring cow ruminant behavior,including video surveillance,sound recognition,and sensor monitoring methods.How‐ever,the application of edge device gives rise to the issue of inadequate real-time performance.To reduce the volume of data transmission and cloud computing workload while achieving real-time monitoring of dairy cow rumination behavior,a real-time monitoring method was proposed for cow ruminant behavior based on edge computing.[Methods]Autono‐mously designed edge devices were utilized to collect and process six-axis acceleration signals from cows in real-time.Based on these six-axis data,two distinct strategies,federated edge intelligence and split edge intelligence,were investigat‐ed for the real-time recognition of cow ruminant behavior.Focused on the real-time recognition method for cow ruminant behavior leveraging federated edge intelligence,the CA-MobileNet v3 network was proposed by enhancing the MobileNet v3 network with a collaborative attention mechanism.Additionally,a federated edge intelligence model was designed uti‐lizing the CA-MobileNet v3 network and the FedAvg federated aggregation algorithm.In the study on split edge intelli‐gence,a split edge intelligence model named MobileNet-LSTM was designed by integrating the MobileNet v3 network with a fusion collaborative attention mechanism and the Bi-LSTM network.[Results and Discussions]Through compara‐tive experiments with MobileNet v3 and MobileNet-LSTM,the federated edge intelligence model based on CA-Mo‐bileNet v3 achieved an average Precision rate,Recall rate,F1-Score,Specificity,and Accuracy of 97.1%,97.9%,97.5%,98.3%,and 98.2%,respectively,yielding the best recognition performance.[Conclusions]It is provided a real-time and effective method for monitoring cow ruminant behavior,and the proposed federated edge intelligence model can be ap‐plied in practical settings. 展开更多
关键词 cow rumination behavior real-time monitoring edge computing improved MobileNet v3 edge intelligence model Bi-LSTM
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Performance Evaluation of Three-Dimensional UWB Real-Time Locating Auto-Positioning System for Fire Rescue
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作者 Hang Yang Xunbo Li Witold Pedrycz 《Intelligent Automation & Soft Computing》 SCIE 2023年第9期3039-3058,共20页
Fire rescue challenges and solutions have evolved from straightfor-ward plane rescue to encompass 3D space due to the rise of high-rise city buildings.Hence,this study facilitates a system with quick and simplified on... Fire rescue challenges and solutions have evolved from straightfor-ward plane rescue to encompass 3D space due to the rise of high-rise city buildings.Hence,this study facilitates a system with quick and simplified on-site launching and generates real-time location data,enabling fire rescuers to arrive at the intended spot faster and correctly for effective and precise rescue.Auto-positioning with step-by-step instructions is proposed when launching the locating system,while no extra measuring instrument like Total Station(TS)is needed.Real-time location tracking is provided via a 3D space real-time locating system(RTLS)constructed using Ultra-wide Bandwidth technology(UWB),which requires electromagnetic waves to pass through concrete walls.A hybrid weighted least squares with a time difference of arrival(WLS/TDOA)positioning method is proposed to address real path-tracking issues in 3D space and to meet RTLS requirements for quick computing in real-world applications.The 3D WLS/TDOA algorithm is theoretically constructed with the Cramer-Rao lower bound(CRLB).The computing complexity is reduced to the lower bound for embedded hardware to directly compute the time differential of the arriving signals using the time-to-digital converter(TDC).The results of the experiments show that the errors are controlled when the positioning algorithm is applied in various complicated situations to fulfill the requirements of engineering applications.The statistical analysis of the data reveals that the proposed UWB RTLS auto-positioning system can track target tags with an accuracy of 0.20 m. 展开更多
关键词 3D space positioning ULTRA-WIDEBAND real-time locating system time difference of arrival Cramer-Rao lower bound fire rescue
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Lie symmetry analysis and invariant solutions for the(3+1)-dimensional Virasoro integrable model
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作者 胡恒春 李雅琦 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第4期249-254,共6页
Lie symmetry analysis is applied to a(3+1)-dimensional Virasoro integrable model and the corresponding similarity reduction equations are obtained with the different infinitesimal generators.Invariant solutions with a... Lie symmetry analysis is applied to a(3+1)-dimensional Virasoro integrable model and the corresponding similarity reduction equations are obtained with the different infinitesimal generators.Invariant solutions with arbitrary functions for the(3+1)-dimensional Virasoro integrable model,including the interaction solution between a kink and a soliton,the lump-type solution and periodic solutions,have been studied analytically and graphically. 展开更多
关键词 (3+1)-dimensional Virasoro integrable model Lie symmetry invariant solutions
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A survey of real-time rendering on Web3D application
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作者 Geng YU Chang LIU +7 位作者 Ting FANG Jinyuan JIA Enming LIN Yiqiang HE Siyuan FU Long WANG Lei WEI Qingyu HUANG 《Virtual Reality & Intelligent Hardware》 EI 2023年第5期379-394,共16页
Background In recent years, with the rapid development of mobile Internet and Web3D technologies, a large number of web-based online 3D visualization applications have emerged. Web3D applications, including Web3D onli... Background In recent years, with the rapid development of mobile Internet and Web3D technologies, a large number of web-based online 3D visualization applications have emerged. Web3D applications, including Web3D online tourism, Web3D online architecture, Web3D online education environment, Web3D online medical care, and Web3D online shopping are examples of these applications that leverage 3D rendering on the web. These applications have pushed the boundaries of traditional web applications that use text, sound, image, video, and 2D animation as their main communication media, and resorted to 3D virtual scenes as the main interaction object, enabling a user experience that delivers a strong sense of immersion. This paper approached the emerging Web3D applications that generate stronger impacts on people's lives through “real-time rendering technology”, which is the core technology of Web3D. This paper discusses all the major 3D graphics APIs of Web3D and the well-known Web3D engines at home and abroad and classify the real-time rendering frameworks of Web3D applications into different categories. Results Finally, this study analyzed the specific demand posed by different fields to Web3D applications by referring to the representative Web3D applications in each particular field. Conclusions Our survey results show that Web3D applications based on real-time rendering have in-depth sectors of society and even family, which is a trend that has influence on every line of industry. 展开更多
关键词 WEB3D real-time rendering Virtual reality Cloud rendering Mobile Internet
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Interaction solutions for the second extended(3+1)-dimensional Jimbo–Miwa equation
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作者 马红彩 毛雪 邓爱平 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第6期112-121,共10页
Based on the Hirota bilinear method,the second extended(3+1)-dimensional Jimbo–Miwa equation is established.By Maple symbolic calculation,lump and lump-kink soliton solutions are obtained.The interaction solutions be... Based on the Hirota bilinear method,the second extended(3+1)-dimensional Jimbo–Miwa equation is established.By Maple symbolic calculation,lump and lump-kink soliton solutions are obtained.The interaction solutions between the lump and multi-kink soliton,and the interaction between the lump and triangular periodic soliton are derived by combining a multi-exponential function or trigonometric sine and cosine functions with quadratic functions.Furthermore,periodiclump wave solution is derived via the ansatz including hyperbolic and trigonometric functions.Finally,3D plots,2D curves,density plots,and contour plots with particular choices of the suitable parameters are depicted to illustrate the dynamical features of these solutions. 展开更多
关键词 Hirota bilinear method second extended(3+1)-dimensional Jimbo–Miwa equation lump solution interaction solution
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A 3-DIMENSIONAL DATA MODEL FOR VISUALIZING CLOVERLEAF JUNCTION IN A CITY MODEL 被引量:6
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作者 Chen Jun Sun Min Zhou Qiming 《Geo-Spatial Information Science》 1999年第1期9-15,共7页
The research work has been seldom done about cloverleaf junction expression in a 3-dimensional city model (3DCM). The main reason is that the cloverleaf junction is often in a complex and enormous construction. Its ma... The research work has been seldom done about cloverleaf junction expression in a 3-dimensional city model (3DCM). The main reason is that the cloverleaf junction is often in a complex and enormous construction. Its main body is bestraddle in air,and has aerial intersections between its parts. This complex feature made cloverleaf junction quite different from buildings and terrain, therefore, it is difficult to express this kind of spatial objects in the same way as for buildings and terrain. In this paper,authors analyze spatial characteristics of cloverleaf junction, propose an all-constraint points TIN algorithm to partition cloverleaf junction road surface, and develop a method to visualize cloverleaf junction road surface using TIN. In order to manage cloverleaf junction data efficiently, the authors also analyzed the mechanism of 3DCM data management, extended BLOB type in relational database, and combined R-tree index to manage 3D spatial data. Based on this extension, an appropriate data 展开更多
关键词 3-dimensional CITY model (3DCM) GIS cloverleaf JUNCTION DATA STRUCTURE DATABASE
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All Single Traveling Wave Solutions to (3+1)-Dimensional Nizhnok-Novikov-Veselov Equation 被引量:12
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作者 LIU Cheng-Shi 《Communications in Theoretical Physics》 SCIE CAS CSCD 2006年第6期991-992,共2页
Using elementary integral method, a complete classification of all possible exact traveling wave solutions to (3+1)-dimensional Nizhnok-Novikov-Veselov equation is given. Some solutions are new.
关键词 3+1)-dimensional Nizhnok-Novikov-Veselov equation traveling wave solution elementary integral method
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New Exact Solutions and Conservation Laws to (3+1)-Dimensional Potential-YTSF Equation 被引量:10
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作者 ZHANG Li-Hua LIU Xi-Qiang 《Communications in Theoretical Physics》 SCIE CAS CSCD 2006年第3期487-492,共6页
Using the modified find some new exact solutions to Lie point symmetry groups and also get conservation laws, of the CK's direct method, we build the relationship between new solutions and old ones and the (3+1)-d... Using the modified find some new exact solutions to Lie point symmetry groups and also get conservation laws, of the CK's direct method, we build the relationship between new solutions and old ones and the (3+1)-dimensional potentiaial-YTSF equation. Baaed on the invariant group theory, Lie symmetries of the (3+1)-dimensional potential-YTSF equation are obtained. We equation with the given Lie symmetry. 展开更多
关键词 new exact solutions Lie point symmetry groups conservation laws 3+1)-dimensional potential-YTSF equation
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Evaluation of Airway Obstruction at Soft Palate Level in Male Patients with Obstructive Sleep Apnea/Hypopnea Syndrome:Dynamic 3-Dimensional CT Imaging of Upper Airway 被引量:10
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作者 肖英 陈雄 +4 位作者 史河水 杨阳 何烈纯 董家琪 孔维佳 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2011年第3期413-418,共6页
This study examined the dynamic characteristics of upper airway collapse at soft palate level in patients with obstructive sleep apnea/hypopnea syndrome(OSAHS) by using dynamic 3-Dimensional(3-D) CT imaging.A tota... This study examined the dynamic characteristics of upper airway collapse at soft palate level in patients with obstructive sleep apnea/hypopnea syndrome(OSAHS) by using dynamic 3-Dimensional(3-D) CT imaging.A total of 41 male patients who presented with 2 of the following symptoms,i.e.,daytime sleepiness and fatigue,frequent snoring,and apnea with witness,were diagnosed as having OSAHS.They underwent full-night polysomnography and then dynamic 3-D CT imaging of the upper airway during quiet breathing and in Muller's maneuver.The soft palate length(SPL),the minimal cross-sectional area of the retropalatal region(mXSA-RP),and the vertical distance from the hard palate to the upper posterior part of the hyoid(hhL) were compared between the two breathing states.These parameters,together with hard palate length(HPL),were also compared between mild/moderate and severe OSAHS groups.Association of these parameters with the severity of OSAHS [as reflected by apnea hypopnea index(AHI) and the lowest saturation of blood oxygen(LSaO2)] was examined.The results showed that 31 patients had severe OSAHS,and 10 mild/moderate OSAHS.All the patients had airway obstruction at soft palate level.mXSA-RP was significantly decreased and SPL remarkably increased during Muller's maneuver as compared with the quiet breathing state.There were no significant differences in these airway parameters(except the position of the hyoid bone) between severe and mild/moderate OSAHS groups.And no significant correlation between these airway parameters and the severity of OSAHS was found.The position of hyoid was lower in the severe OSAHS group than in the mild/moderate OSAHS group.The patients in group with body mass index(BMI)≥26 had higher collapse ratio of mXSA-RP,greater neck circumference and smaller mXSA-RP in the Muller's maneuver than those in group with BMI26(P0.05 for all).It was concluded that dynamic 3-D CT imaging could dynamically show the upper airway changes at soft palate level in OSAHS patients.All the OSAHS patients had airway obstruction of various degrees at soft palate level.But no correlation was observed between the airway change at soft palate level and the severity of OSAHS.The patients in group with BMI≥26 were more likely to develop airway obstruction at soft palate level than those with BMI26. 展开更多
关键词 obstructive sleep apnea/hypopnea syndrome upper airway obstruction soft palate level dynamic computed tomography 3-dimensional imaging
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Grammian Determinant Solution and Pfaffianization for a (3+1)-Dimensional Soliton Equation 被引量:5
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作者 WU Jian-Ping GENG Xian-Guo2 《Communications in Theoretical Physics》 SCIE CAS CSCD 2009年第11期791-794,共4页
Based on the Pfaffian derivative formulae,a Grammian determinant solution for a(3+1)-dimensionalsoliton equation is obtained.Moreover,the Pfaffianization procedure is applied for the equation to generate a newcoupled ... Based on the Pfaffian derivative formulae,a Grammian determinant solution for a(3+1)-dimensionalsoliton equation is obtained.Moreover,the Pfaffianization procedure is applied for the equation to generate a newcoupled system.At last,a Gram-type Pfaffian solution to the new coupled system is given. 展开更多
关键词 3+1)-dimensional soliton equation Grammian determinant solution PFAFFIANIZATION Gram-type Pfaffian solution
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NOVEL 6-SPS PARALLEL 3-DIMENSIONAL PLATFORM MANIPULATOR AND ITS FORCE/MOTION TRANSMISSION ANALYSIS 被引量:2
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作者 Jin Zhenlin School of Mechanical Engineering, Yanshan University, Qinhuangdao 066004, ChinaGao Feng Hebei University of Technology 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2002年第4期298-302,共5页
The unique design for a novel 6-SPS parallel 3-dimensional platformmanipulator with an orthogonal configuration is investigated. The layout feature of the parallelmanipulator is described. Its force/motion transmissio... The unique design for a novel 6-SPS parallel 3-dimensional platformmanipulator with an orthogonal configuration is investigated. The layout feature of the parallelmanipulator is described. Its force/motion transmission capability, evaluation criteria arepresented. At the orthogonal configuration, the criteria and the relationships between the criteriaand the link lengths are analyzed, which is important since it can provide designer a piece ofvaluable information about how to choose the linear actuators. From the analysis of the results itis shown that the force/motion transmission capabilities of the parallel manipulator arecharacterized by isotropy at the orthogonal configuration. The manipulator is particularly suitablefor certain applications in 6-DOF micromanipulators and 6-axis force/moment transducers. 展开更多
关键词 6-SPS parallel manipulator 3-dimensional platform manipulator ISOTROPY
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Spatiotemporal Similaritons in (3+l)-Dimensional Inhomogeneous Nonlinear Medium with Cubic-Quintic Nonlinearity 被引量:3
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作者 陈翼翔 陆璇辉 《Communications in Theoretical Physics》 SCIE CAS CSCD 2011年第5期871-877,共7页
We obtain exact spatiotemporal similaritons to a (3+ l)-dimensional inhomogeneous nonlinear Schrodinger equation, which describes the propagation of optical pulses in a cubic-quintic nonlinearity medium with distri... We obtain exact spatiotemporal similaritons to a (3+ l)-dimensional inhomogeneous nonlinear Schrodinger equation, which describes the propagation of optical pulses in a cubic-quintic nonlinearity medium with distributed dispersion and gain. A one-to-one correspondence between such self-similar waves and solutions of the elliptic equation is found when a certain compatibility condition is satisfied. Based on exact solutions, we discuss evolutional behaviors of self-similar cnoidal waves and chirped similaritons in two kind of typicai soliton control systems. Moreover, the comparison between chirped similaritons and chirp-free solitons is given. 展开更多
关键词 spatiotemporal similaritons 3+l)-dimensional cubic-quintic nonlinear SchrSdinger equation dynamical behaviors
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Some Special Types of Solitary Wave Solutions for (3+1)-Dimensional Jimbo-MiwaEquation 被引量:3
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作者 BAICheng-Lin ZHAOHong 《Communications in Theoretical Physics》 SCIE CAS CSCD 2004年第6期875-877,共3页
Using the extended homogeneous balance method, we find some special types of single solitary wave solution and new types of the multisoliton solutions of the (3+1)-dimensional Jimbo-Miwa equation.
关键词 (3+1)-dimensional Jimbo-Miwa equation extended homogeneous balance method soliton solutions
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A Generalized Variable-Coefficient Algebraic Method Exactly Solving (3+1)-Dimensional Kadomtsev-Petviashvilli Equation 被引量:3
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作者 BAI Cheng-Lin BAI Cheng-Jie ZHAO Hong 《Communications in Theoretical Physics》 SCIE CAS CSCD 2005年第5X期821-826,共6页
A generalized variable-coefficient algebraic method is appfied to construct several new families of exact solutions of physical interest for (3+1)-dimensional Kadomtsev-Petviashvilli (KP) equation. Among them, th... A generalized variable-coefficient algebraic method is appfied to construct several new families of exact solutions of physical interest for (3+1)-dimensional Kadomtsev-Petviashvilli (KP) equation. Among them, the Jacobi elliptic periodic solutions exactly degenerate to the soliton solutions at a certain limit condition. Compared with the existing tanh method, the extended tanh method, the Jacobi elliptic function method, and the algebraic method, the proposed method gives new and more general solutions. 展开更多
关键词 generalized variable-coefficient algebraic method 3+1)-dimensional KP equation exact explicit solutions
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Residual symmetry, CRE integrability and interaction solutions of two higher-dimensional shallow water wave equations
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作者 刘希忠 李界通 俞军 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第11期313-319,共7页
Two(3+1)-dimensional shallow water wave equations are studied by using residual symmetry and the consistent Riccati expansion(CRE) method. Through localization of residual symmetries, symmetry reduction solutions of t... Two(3+1)-dimensional shallow water wave equations are studied by using residual symmetry and the consistent Riccati expansion(CRE) method. Through localization of residual symmetries, symmetry reduction solutions of the two equations are obtained. The CRE method is applied to the two equations to obtain new B?cklund transformations from which a type of interesting interaction solution between solitons and periodic waves is generated. 展开更多
关键词 (3+1)-dimensional shallow water wave equation residual symmetry consistent Riccati expansion
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Thermal effect on endurance performance of 3-dimensional RRAM crossbar array 被引量:2
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作者 卢年端 孙鹏霄 +4 位作者 李泠 刘琦 龙世兵 吕杭炳 刘明 《Chinese Physics B》 SCIE EI CAS CSCD 2016年第5期253-257,共5页
Three-dimensional(3D) crossbar array architecture is one of the leading candidates for future ultra-high density nonvolatile memory applications. To realize the technological potential, understanding the reliability... Three-dimensional(3D) crossbar array architecture is one of the leading candidates for future ultra-high density nonvolatile memory applications. To realize the technological potential, understanding the reliability mechanisms of the3 D RRAM array has become a field of intense research. In this work, the endurance performance of the 3D 1D1 R crossbar array under the thermal effect is investigated in terms of numerical simulation. It is revealed that the endurance performance of the 3D 1D1 R array would be seriously deteriorated under thermal effects as the feature size scales down to a relatively small value. A possible method to alleviate the thermal effects is provided and verified by numerical simulation. 展开更多
关键词 3-dimensional resistive random access memory(RRAM) thermal effect endurance performance
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Applications of Extended Mapping Deformation Method in Two (3+1)-Dimensional Nonlinear Models 被引量:1
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作者 HUANGWen-Hua ZHANGJie-Fang GEWei-Kuan 《Communications in Theoretical Physics》 SCIE CAS CSCD 2005年第5期775-780,共6页
Based on the extended mapping deformation method and symbolic computation, many exact travelling wave solutions are found for the (3+1)-dimensional JM equation and the (3+1)-dimensional KP equation. The obtained solut... Based on the extended mapping deformation method and symbolic computation, many exact travelling wave solutions are found for the (3+1)-dimensional JM equation and the (3+1)-dimensional KP equation. The obtained solutions include solitary solution, periodic wave solution, rational travelling wave solution, and Jacobian and Weierstrass function solution, etc. 展开更多
关键词 (3+1)-dimensional JM equation (3+1)-dimensional KP equation travelling wavesolution
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Painleve Analysis and Determinant Solutions of a (3+1)-Dimensional Variable-Coefficient Kadomtsev-Petviashvili Equation in Wronskian and Grammian Form 被引量:2
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作者 MENG Xiang-Hua TIAN Bo +2 位作者 FENG Qian YAO Zhen-Zhi GAO Yi-Tian 《Communications in Theoretical Physics》 SCIE CAS CSCD 2009年第6期1062-1068,共7页
In this paper, the investigation is focused on a (3+1)-dimensional variable-coefficient Kadomtsev- Petviashvili (vcKP) equation, which can describe the realistic nonlinear phenomena in the fluid dynamics and plas... In this paper, the investigation is focused on a (3+1)-dimensional variable-coefficient Kadomtsev- Petviashvili (vcKP) equation, which can describe the realistic nonlinear phenomena in the fluid dynamics and plasma in three spatial dimensions. In order to study the integrability property of such an equation, the Painlevé analysis is performed on it. And then, based on the truncated Painlevé expansion, the bilinear form of the (3+1)-dimensionaJ vcKP equation is obtained under certain coefficients constraint, and its solution in the Wronskian determinant form is constructed and verified by virtue of the Wronskian technique. Besides the Wronskian determinant solution, it is shown that the (3+1)-dimensional vcKP equation also possesses a solution in the form of the Grammian determinant. 展开更多
关键词 3+1)-dimensional variable-coefficient Kadomtsev-Petviashvili equation Painlev@ analysis bilinear form Wronskian determinant Grammian determinant symbolic computation
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Rota-Baxter Operators on 3-dimensional Lie Algebras and Solutions of the Classical Yang-Baxter Equation 被引量:1
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作者 Cheng Yong-sheng Wu Lin-li +1 位作者 Wang Pan-yin Du Xian-kun 《Communications in Mathematical Research》 CSCD 2019年第1期81-96,共16页
In this paper,we compute Rota-Baxter operators on the 3-dimensional Lie algebra g whose derived algebra’s dimension is 2.Furthermore,we give the corresponding solutions of the classical Yang-Baxter equation in the 6-... In this paper,we compute Rota-Baxter operators on the 3-dimensional Lie algebra g whose derived algebra’s dimension is 2.Furthermore,we give the corresponding solutions of the classical Yang-Baxter equation in the 6-dimensional Lie algebras g ■ _(ad~*) g~* and some new structures of left-symmetric algebra induced from g and its Rota-Baxter operators. 展开更多
关键词 Rota-Baxter OPERATORS 3-dimensional Lie ALGEBRA classical Yang-Baxter equation left-symmetric ALGEBRA
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Multimarker Detection of MAGE-1,MAGE-3 and AFP mRNAs by a Real-time Quantitative PCR Assay:a Possible Predictor of Hematogenous Micrometastasis of Hepatocellular Carcinoma 被引量:2
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作者 Yuqing Zhang Qiang Li Ning Liu Tianqiang Song Rende Guo Lingxin Meng Xishan Hao 《Chinese Journal of Clinical Oncology》 CSCD 2008年第2期93-98,共6页
OBJECTIVE To explore the relationship between multimarker detection of MAGE-1,MAGE-3 and AFP mRNAs in the peripheral blood of patients with hepatocellular carcinoma and micrometastasis using a realtime quantitative-PC... OBJECTIVE To explore the relationship between multimarker detection of MAGE-1,MAGE-3 and AFP mRNAs in the peripheral blood of patients with hepatocellular carcinoma and micrometastasis using a realtime quantitative-PCR(real-time Q-PCR)assay. METHODS Peripheral blood samples were obtained from control subjects and 86 patients with hepatocellular carcinoma (HCC).Real-time Q-PCR was used to detect MAGE-1,MAGE-3, and AFP mRNAs in the blood cells. RESULTS In 86 tumor specimens,the positivity for MAGE-1, MAGE-3,and AFP genes was respectively 34.9%(30/86),60.5% (52/86)and 69.8%(60/86).All specimens expressed at least one marker.MAGE-1,MAGE-3,and AFP transcripts were detected respectively in 12(14.0%),18(20.1%)and 29(33.7%)of the 86 blood specimens from hepatocellular carcinoma patients,while 45 specimens(52.3%)were positive for at least one marker.In addition,MAGE-1,MAGE-3 and AFP gene transcripts were not detected in any peripheral blood specimens from 25 chronic liver disease patients and 28 normal healthy volunteers.The positive rate correlated with the TNM clinical stages,extrahepatic metastasis and portal vein carcinothrombosis(P<0.05).No correlation was found between tumor size,tumor number, differentiation,serum a-fetoprotein(AFP)and the positive rate. CONCLUSION Our results indicate that a multimarker real- time Q-PCR assay with cancer-specific markers such as MAGE-1 and MAGE-3 in combination with a hepatocyte-specific AFP marker may be a promising diagnostic tool for monitoring hepatocellular carcinoma patients with better sensitivity and specificity. 展开更多
关键词 real-time Q-PCR. micrometastasis MAGE-1 MAGE-3 AFP hepatocellular carcinoma.
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