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A self-organization formation configuration based assignment probability and collision detection
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作者 SONG Wei WANG Tong +1 位作者 YANG Guangxin ZHANG Peng 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2024年第1期222-232,共11页
The formation control of multiple unmanned aerial vehicles(multi-UAVs)has always been a research hotspot.Based on the straight line trajectory,a multi-UAVs target point assignment algorithm based on the assignment pro... The formation control of multiple unmanned aerial vehicles(multi-UAVs)has always been a research hotspot.Based on the straight line trajectory,a multi-UAVs target point assignment algorithm based on the assignment probability is proposed to achieve the shortest overall formation path of multi-UAVs with low complexity and reduce the energy consumption.In order to avoid the collision between UAVs in the formation process,the concept of safety ball is introduced,and the collision detection based on continuous motion of two time slots and the lane occupation detection after motion is proposed to avoid collision between UAVs.Based on the idea of game theory,a method of UAV motion form setting based on the maximization of interests is proposed,including the maximization of self-interest and the maximization of formation interest is proposed,so that multi-UAVs can complete the formation task quickly and reasonably with the linear trajectory assigned in advance.Finally,through simulation verification,the multi-UAVs target assignment algorithm based on the assignment probability proposed in this paper can effectively reduce the total path length,and the UAV motion selection method based on the maximization interests can effectively complete the task formation. 展开更多
关键词 straight line trajectory assignment probability collision detection lane occupation detection maximization of interests
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Real-Time Detection and Instance Segmentation of Strawberry in Unstructured Environment
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作者 Chengjun Wang Fan Ding +4 位作者 Yiwen Wang Renyuan Wu Xingyu Yao Chengjie Jiang Liuyi Ling 《Computers, Materials & Continua》 SCIE EI 2024年第1期1481-1501,共21页
The real-time detection and instance segmentation of strawberries constitute fundamental components in the development of strawberry harvesting robots.Real-time identification of strawberries in an unstructured envi-r... The real-time detection and instance segmentation of strawberries constitute fundamental components in the development of strawberry harvesting robots.Real-time identification of strawberries in an unstructured envi-ronment is a challenging task.Current instance segmentation algorithms for strawberries suffer from issues such as poor real-time performance and low accuracy.To this end,the present study proposes an Efficient YOLACT(E-YOLACT)algorithm for strawberry detection and segmentation based on the YOLACT framework.The key enhancements of the E-YOLACT encompass the development of a lightweight attention mechanism,pyramid squeeze shuffle attention(PSSA),for efficient feature extraction.Additionally,an attention-guided context-feature pyramid network(AC-FPN)is employed instead of FPN to optimize the architecture’s performance.Furthermore,a feature-enhanced model(FEM)is introduced to enhance the prediction head’s capabilities,while efficient fast non-maximum suppression(EF-NMS)is devised to improve non-maximum suppression.The experimental results demonstrate that the E-YOLACT achieves a Box-mAP and Mask-mAP of 77.9 and 76.6,respectively,on the custom dataset.Moreover,it exhibits an impressive category accuracy of 93.5%.Notably,the E-YOLACT also demonstrates a remarkable real-time detection capability with a speed of 34.8 FPS.The method proposed in this article presents an efficient approach for the vision system of a strawberry-picking robot. 展开更多
关键词 YOLACT real-time detection instance segmentation attention mechanism STRAWBERRY
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Reachability-Based Confidence-Aware Probabilistic Collision Detection in Highway Driving
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作者 Xinwei Wang Zirui Li +1 位作者 Javier Alonso-Mora Meng Wang 《Engineering》 SCIE EI CAS CSCD 2024年第2期90-107,共18页
Risk assessment is a crucial component of collision warning and avoidance systems for intelligent vehicles.Reachability-based formal approaches have been developed to ensure driving safety to accurately detect potenti... Risk assessment is a crucial component of collision warning and avoidance systems for intelligent vehicles.Reachability-based formal approaches have been developed to ensure driving safety to accurately detect potential vehicle collisions.However,they suffer from over-conservatism,potentially resulting in false–positive risk events in complicated real-world applications.In this paper,we combine two reachability analysis techniques,a backward reachable set(BRS)and a stochastic forward reachable set(FRS),and propose an integrated probabilistic collision–detection framework for highway driving.Within this framework,we can first use a BRS to formally check whether a two-vehicle interaction is safe;otherwise,a prediction-based stochastic FRS is employed to estimate the collision probability at each future time step.Thus,the framework can not only identify non-risky events with guaranteed safety but also provide accurate collision risk estimation in safety-critical events.To construct the stochastic FRS,we develop a neural network-based acceleration model for surrounding vehicles and further incorporate a confidence-aware dynamic belief to improve the prediction accuracy.Extensive experiments were conducted to validate the performance of the acceleration prediction model based on naturalistic highway driving data.The efficiency and effectiveness of the framework with infused confidence beliefs were tested in both naturalistic and simulated highway scenarios.The proposed risk assessment framework is promising for real-world applications. 展开更多
关键词 Probabilistic collision detection Confidence awareness Probabilistic acceleration prediction Reachability analysis Risk assessment
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Real-Time Object Detection and Face Recognition Application for the Visually Impaired
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作者 Karshiev Sanjar Soyoun Bang +1 位作者 SookheeRyue Heechul Jung 《Computers, Materials & Continua》 SCIE EI 2024年第6期3569-3583,共15页
The advancement of navigation systems for the visually impaired has significantly enhanced their mobility by mitigating the risk of encountering obstacles and guiding them along safe,navigable routes.Traditional appro... The advancement of navigation systems for the visually impaired has significantly enhanced their mobility by mitigating the risk of encountering obstacles and guiding them along safe,navigable routes.Traditional approaches primarily focus on broad applications such as wayfinding,obstacle detection,and fall prevention.However,there is a notable discrepancy in applying these technologies to more specific scenarios,like identifying distinct food crop types or recognizing faces.This study proposes a real-time application designed for visually impaired individuals,aiming to bridge this research-application gap.It introduces a system capable of detecting 20 different food crop types and recognizing faces with impressive accuracies of 83.27%and 95.64%,respectively.These results represent a significant contribution to the field of assistive technologies,providing visually impaired users with detailed and relevant information about their surroundings,thereby enhancing their mobility and ensuring their safety.Additionally,it addresses the vital aspects of social engagements,acknowledging the challenges faced by visually impaired individuals in recognizing acquaintances without auditory or tactile signals,and highlights recent developments in prototype systems aimed at assisting with face recognition tasks.This comprehensive approach not only promises enhanced navigational aids but also aims to enrich the social well-being and safety of visually impaired communities. 展开更多
关键词 Artificial intelligence deep learning real-time object detection application
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A CNN-Based Single-Stage Occlusion Real-Time Target Detection Method
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作者 Liang Liu Nan Yang +4 位作者 Saifei Liu Yuanyuan Cao Shuowen Tian Tiancheng Liu Xun Zhao 《Journal of Intelligent Learning Systems and Applications》 2024年第1期1-11,共11页
Aiming at the problem of low accuracy of traditional target detection methods for target detection in endoscopes in substation environments, a CNN-based real-time detection method for masked targets is proposed. The m... Aiming at the problem of low accuracy of traditional target detection methods for target detection in endoscopes in substation environments, a CNN-based real-time detection method for masked targets is proposed. The method adopts the overall design of backbone network, detection network and algorithmic parameter optimisation method, completes the model training on the self-constructed occlusion target dataset, and adopts the multi-scale perception method for target detection. The HNM algorithm is used to screen positive and negative samples during the training process, and the NMS algorithm is used to post-process the prediction results during the detection process to improve the detection efficiency. After experimental validation, the obtained model has the multi-class average predicted value (mAP) of the dataset. It has general advantages over traditional target detection methods. The detection time of a single target on FDDB dataset is 39 ms, which can meet the need of real-time target detection. In addition, the project team has successfully deployed the method into substations and put it into use in many places in Beijing, which is important for achieving the anomaly of occlusion target detection. 展开更多
关键词 real-time Mask Target CNN (Convolutional Neural Network) Single-Stage detection Multi-Scale Feature Perception
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Force Sensitive Resistors-Based Real-Time Posture Detection System Using Machine Learning Algorithms
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作者 Arsal Javaid Areeb Abbas +4 位作者 Jehangir Arshad Mohammad Khalid Imam Rahmani Sohaib Tahir Chauhdary Mujtaba Hussain Jaffery Abdulbasid S.Banga 《Computers, Materials & Continua》 SCIE EI 2023年第11期1795-1814,共20页
To detect the improper sitting posture of a person sitting on a chair,a posture detection system using machine learning classification has been proposed in this work.The addressed problem correlates to the third Susta... To detect the improper sitting posture of a person sitting on a chair,a posture detection system using machine learning classification has been proposed in this work.The addressed problem correlates to the third Sustainable Development Goal(SDG),ensuring healthy lives and promoting well-being for all ages,as specified by the World Health Organization(WHO).An improper sitting position can be fatal if one sits for a long time in the wrong position,and it can be dangerous for ulcers and lower spine discomfort.This novel study includes a practical implementation of a cushion consisting of a grid of 3×3 force-sensitive resistors(FSR)embedded to read the pressure of the person sitting on it.Additionally,the Body Mass Index(BMI)has been included to increase the resilience of the system across individual physical variances and to identify the incorrect postures(backward,front,left,and right-leaning)based on the five machine learning algorithms:ensemble boosted trees,ensemble bagged trees,ensemble subspace K-Nearest Neighbors(KNN),ensemble subspace discriminant,and ensemble RUSBoosted trees.The proposed arrangement is novel as existing works have only provided simulations without practical implementation,whereas we have implemented the proposed design in Simulink.The results validate the proposed sensor placements,and the machine learning(ML)model reaches a maximum accuracy of 99.99%,which considerably outperforms the existing works.The proposed concept is valuable as it makes it easier for people in workplaces or even at individual household levels to work for long periods without suffering from severe harmful effects from poor posture. 展开更多
关键词 Posture detection FSR sensor machine learning real-time KNN
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Towards Cache-Assisted Hierarchical Detection for Real-Time Health Data Monitoring in IoHT
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作者 Muhammad Tahir Mingchu Li +4 位作者 Irfan Khan Salman AAl Qahtani Rubia Fatima Javed Ali Khan Muhammad Shahid Anwar 《Computers, Materials & Continua》 SCIE EI 2023年第11期2529-2544,共16页
Real-time health data monitoring is pivotal for bolstering road services’safety,intelligence,and efficiency within the Internet of Health Things(IoHT)framework.Yet,delays in data retrieval can markedly hinder the eff... Real-time health data monitoring is pivotal for bolstering road services’safety,intelligence,and efficiency within the Internet of Health Things(IoHT)framework.Yet,delays in data retrieval can markedly hinder the efficacy of big data awareness detection systems.We advocate for a collaborative caching approach involving edge devices and cloud networks to combat this.This strategy is devised to streamline the data retrieval path,subsequently diminishing network strain.Crafting an adept cache processing scheme poses its own set of challenges,especially given the transient nature of monitoring data and the imperative for swift data transmission,intertwined with resource allocation tactics.This paper unveils a novel mobile healthcare solution that harnesses the power of our collaborative caching approach,facilitating nuanced health monitoring via edge devices.The system capitalizes on cloud computing for intricate health data analytics,especially in pinpointing health anomalies.Given the dynamic locational shifts and possible connection disruptions,we have architected a hierarchical detection system,particularly during crises.This system caches data efficiently and incorporates a detection utility to assess data freshness and potential lag in response times.Furthermore,we introduce the Cache-Assisted Real-Time Detection(CARD)model,crafted to optimize utility.Addressing the inherent complexity of the NP-hard CARD model,we have championed a greedy algorithm as a solution.Simulations reveal that our collaborative caching technique markedly elevates the Cache Hit Ratio(CHR)and data freshness,outshining its contemporaneous benchmark algorithms.The empirical results underscore the strength and efficiency of our innovative IoHT-based health monitoring solution.To encapsulate,this paper tackles the nuances of real-time health data monitoring in the IoHT landscape,presenting a joint edge-cloud caching strategy paired with a hierarchical detection system.Our methodology yields enhanced cache efficiency and data freshness.The corroborative numerical data accentuates the feasibility and relevance of our model,casting a beacon for the future trajectory of real-time health data monitoring systems. 展开更多
关键词 real-time health data monitoring Cache-Assisted real-time detection(CARD) edge-cloud collaborative caching scheme hierarchical detection Internet of Health Things(IoHT)
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LDA-ID:An LDA-Based Framework for Real-Time Network Intrusion Detection
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作者 Weidong Zhou Shengwei Lei +1 位作者 Chunhe Xia Tianbo Wang 《China Communications》 SCIE CSCD 2023年第12期166-181,共16页
Network intrusion poses a severe threat to the Internet.However,existing intrusion detection models cannot effectively distinguish different intrusions with high-degree feature overlap.In addition,efficient real-time ... Network intrusion poses a severe threat to the Internet.However,existing intrusion detection models cannot effectively distinguish different intrusions with high-degree feature overlap.In addition,efficient real-time detection is an urgent problem.To address the two above problems,we propose a Latent Dirichlet Allocation topic model-based framework for real-time network Intrusion Detection(LDA-ID),consisting of static and online LDA-ID.The problem of feature overlap is transformed into static LDA-ID topic number optimization and topic selection.Thus,the detection is based on the latent topic features.To achieve efficient real-time detection,we design an online computing mode for static LDA-ID,in which a parameter iteration method based on momentum is proposed to balance the contribution of prior knowledge and new information.Furthermore,we design two matching mechanisms to accommodate the static and online LDA-ID,respectively.Experimental results on the public NSL-KDD and UNSW-NB15 datasets show that our framework gets higher accuracy than the others. 展开更多
关键词 feature overlap LDA-ID optimal topic number determination real-time intrusion detection
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Portable FBAR based E-nose for cold chain real-time bananas shelf time detection
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作者 Chen Wu Jiuyan Li 《Nanotechnology and Precision Engineering》 CAS CSCD 2023年第1期32-39,共8页
Being cheap,nondestructive,and easy to use,gas sensors play important roles in the food industry.However,most gas sensors are suitable more for laboratory-quality fast testing rather than for cold-chain continuous and... Being cheap,nondestructive,and easy to use,gas sensors play important roles in the food industry.However,most gas sensors are suitable more for laboratory-quality fast testing rather than for cold-chain continuous and cumulative testing.Also,an ideal electronic nose(E-nose)in a cold chain should be stable to its surroundings and remain highly accurate and portable.In this work,a portable film bulk acoustic resonator(FBAR)-based E-nose was built for real-time measurement of banana shelf time.The sensor chamber to contain the portable circuit of the E-nose is as small as a smartphone,and by introducing an air-tight FBAR as a reference,the E-nose can avoid most of the drift caused by surroundings.With the help of porous layer by layer(LBL)coating of the FBAR,the sensitivity of the E-nose is 5 ppm to ethylene and 0.5 ppm to isoamyl acetate and isoamyl butyrate,while the detection range is large enough to cover a relative humidity of 0.8.In this regard,the E-nose can easily discriminate between yellow bananas with green necks and entirely yellow bananas while allowing the bananas to maintain their biological activities in their normal storage state,thereby showing the possibility of real-time shelf time detection.This portable FBAR-based E-nose has a large testing scale,high sensitivity,good humidity tolerance,and low frequency drift to its surroundings,thereby meeting the needs of cold-chain usage. 展开更多
关键词 Film bulk acoustic resonator(FBAR) Portable E-nose real-time detection Layer by layer
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NFA:A neural factorization autoencoder based online telephony fraud detection
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作者 Abdul Wahid Mounira Msahli +1 位作者 Albert Bifet Gerard Memmi 《Digital Communications and Networks》 SCIE CSCD 2024年第1期158-167,共10页
The proliferation of internet communication channels has increased telecom fraud,causing billions of euros in losses for customers and the industry each year.Fraudsters constantly find new ways to engage in illegal ac... The proliferation of internet communication channels has increased telecom fraud,causing billions of euros in losses for customers and the industry each year.Fraudsters constantly find new ways to engage in illegal activity on the network.To reduce these losses,a new fraud detection approach is required.Telecom fraud detection involves identifying a small number of fraudulent calls from a vast amount of call traffic.Developing an effective strategy to combat fraud has become challenging.Although much effort has been made to detect fraud,most existing methods are designed for batch processing,not real-time detection.To solve this problem,we propose an online fraud detection model using a Neural Factorization Autoencoder(NFA),which analyzes customer calling patterns to detect fraudulent calls.The model employs Neural Factorization Machines(NFM)and an Autoencoder(AE)to model calling patterns and a memory module to adapt to changing customer behaviour.We evaluate our approach on a large dataset of real-world call detail records and compare it with several state-of-the-art methods.Our results show that our approach outperforms the baselines,with an AUC of 91.06%,a TPR of 91.89%,an FPR of 14.76%,and an F1-score of 95.45%.These results demonstrate the effectiveness of our approach in detecting fraud in real-time and suggest that it can be a valuable tool for preventing fraud in telecommunications networks. 展开更多
关键词 Telecom industry Streaming anomaly detection Fraud analysis Factorization machine real-time system Security
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Cyber Resilience through Real-Time Threat Analysis in Information Security
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作者 Aparna Gadhi Ragha Madhavi Gondu +1 位作者 Hitendra Chaudhary Olatunde Abiona 《International Journal of Communications, Network and System Sciences》 2024年第4期51-67,共17页
This paper examines how cybersecurity is developing and how it relates to more conventional information security. Although information security and cyber security are sometimes used synonymously, this study contends t... This paper examines how cybersecurity is developing and how it relates to more conventional information security. Although information security and cyber security are sometimes used synonymously, this study contends that they are not the same. The concept of cyber security is explored, which goes beyond protecting information resources to include a wider variety of assets, including people [1]. Protecting information assets is the main goal of traditional information security, with consideration to the human element and how people fit into the security process. On the other hand, cyber security adds a new level of complexity, as people might unintentionally contribute to or become targets of cyberattacks. This aspect presents moral questions since it is becoming more widely accepted that society has a duty to protect weaker members of society, including children [1]. The study emphasizes how important cyber security is on a larger scale, with many countries creating plans and laws to counteract cyberattacks. Nevertheless, a lot of these sources frequently neglect to define the differences or the relationship between information security and cyber security [1]. The paper focus on differentiating between cybersecurity and information security on a larger scale. The study also highlights other areas of cybersecurity which includes defending people, social norms, and vital infrastructure from threats that arise from online in addition to information and technology protection. It contends that ethical issues and the human factor are becoming more and more important in protecting assets in the digital age, and that cyber security is a paradigm shift in this regard [1]. 展开更多
关键词 Cybersecurity Information Security Network Security Cyber Resilience real-time Threat Analysis Cyber Threats Cyberattacks Threat Intelligence Machine Learning Artificial Intelligence Threat detection Threat Mitigation Risk Assessment Vulnerability Management Incident Response Security Orchestration Automation Threat Landscape Cyber-Physical Systems Critical Infrastructure Data Protection Privacy Compliance Regulations Policy Ethics CYBERCRIME Threat Actors Threat Modeling Security Architecture
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Real-Time Detection of Cracks on Concrete Bridge Decks Using Deep Learning in the Frequency Domain 被引量:9
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作者 Qianyun Zhang Kaveh Barri +1 位作者 Saeed K.Babanajad Amir H.Alavi 《Engineering》 SCIE EI 2021年第12期1786-1796,共11页
This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequen... This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequency domain.The so-called 1D-CNN-LSTM algorithm is trained using thousands of images of cracked and non-cracked concrete bridge decks.In order to improve the training efficiency,images are first transformed into the frequency domain during a preprocessing phase.The algorithm is then calibrated using the flattened frequency data.LSTM is used to improve the performance of the developed network for long sequence data.The accuracy of the developed model is 99.05%,98.9%,and 99.25%,respectively,for training,validation,and testing data.An implementation framework is further developed for future application of the trained model for large-scale images.The proposed 1D-CNN-LSTM method exhibits superior performance in comparison with existing deep learning methods in terms of accuracy and computation time.The fast implementation of the 1D-CNN-LSTM algorithm makes it a promising tool for real-time crack detection. 展开更多
关键词 Crack detection Concrete bridge deck Deep learning real-time
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A fast and adaptive method for automatic weld defect detection in various real-time X-ray imaging systems 被引量:10
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作者 邵家鑫 都东 +2 位作者 石涵 常保华 郭桂林 《China Welding》 EI CAS 2012年第1期8-12,共5页
A first and effective method is proposed to detect weld deject adaptively in various Dypes of real-time X-ray images obtained in different conditions. After weld extraction and noise reduction, a proper template of me... A first and effective method is proposed to detect weld deject adaptively in various Dypes of real-time X-ray images obtained in different conditions. After weld extraction and noise reduction, a proper template of median filter is used to estimate the weld background. After the weld background is subtracted from the original image, an adaptite threshold segmentation algorithm is proposed to obtain the binary image, and then the morphological close and open operation, labeling algorithm and fids'e alarm eliminating algorithm are applied to pracess the binary image to obtain the defect, ct detection result. At last, a fast realization procedure jbr proposed method is developed. The proposed method is tested in real-time X-ray image,s obtairted in different X-ray imaging sutems. Experiment results show that the proposed method is effective to detect low contrast weld dejects with few .false alarms and is adaptive to various types of real-time X-ray imaging systems. 展开更多
关键词 non-destructive testing real-time X-ray imaging weld defect automatie detection
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Real-time Fluorescence PCR Method for Detection of Burkholderia glumae from Rice 被引量:5
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作者 FANG Yuan XU Li-hui TIAN Wen-xiao HUAI Yan YU Shan-hong LOU Miao-miao XIE Guan-lin 《Rice science》 SCIE 2009年第2期157-160,共4页
Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It's quite necessary to set up effective detection methods for the pathogen to manage further ... Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It's quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×10^4 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. B. glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1:100. 展开更多
关键词 Burkholderia glumae bacterial grain rot detection real-time fluorescence polymerase chain reaction DCE
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Collision detection of virtual plant based on bounding volume hierarchy: A case study on virtual wheat 被引量:6
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作者 TANG Liang SONG Wei-guo +3 位作者 HOU Tian-cheng LIU Lei-lei CAO Wei-xing ZHU Yan 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第2期306-314,共9页
Visualization of simulated crop growth and development is of significant interest to crop research and production. This study aims to address the phenomenon of organs cross-drawing by developing a method of collision ... Visualization of simulated crop growth and development is of significant interest to crop research and production. This study aims to address the phenomenon of organs cross-drawing by developing a method of collision detection for improving vivid 3D visualizations of virtual wheat crops. First, the triangular data of leaves are generated with the tessellation of non-uniform rational B-splines surfaces. Second, the bounding volumes(BVs) and bounding volume hierarchies(BVHs) of leaves are constructed based on the leaf morphological characteristics and the collision detection of two leaves are performed using the Separating Axis Theorem. Third, the detecting effect of the above method is compared with the methods of traditional BVHs, Axis-Aligned Bounding Box(AABB) tree, and Oriented Bounding Box(OBB) tree. Finally, the BVs of other organs(ear, stem, and leaf sheath) in virtual wheat plant are constructed based on their geometric morphology, and the collision detections are conducted at the organ, individual and population scales. The results indicate that the collision detection method developed in this study can accurately detect collisions between organs, especially at the plant canopy level with high collision frequency. This collision detection-based virtual crop visualization method could reduce the phenomenon of organs cross-drawing effectively and enhance the reality of visualizations. 展开更多
关键词 wheat collision detection bounding volume hierarchy virtual plant morphology
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Development and validation of a TaqMan^(TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda 被引量:2
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作者 XIE Guosi HUANG Jie +3 位作者 ZHANG Qingli HAN Nana SHI Chengyin WANG Xiuhua 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2012年第4期140-148,共9页
Edwardsiella tarda is one of the most important emerging pathogens in tile global aquaculture industries. As such, an accurate diagnosis and quantitative analytical methods are urgently needed for this bacterium. In t... Edwardsiella tarda is one of the most important emerging pathogens in tile global aquaculture industries. As such, an accurate diagnosis and quantitative analytical methods are urgently needed for this bacterium. In this study, primers and a TaqMan probe specific to the conservative sequences of the 16S rRNA gene of E. tarda were designed. The concentration of primers and TaqMan probe were optimized to 200 nmol/L and 120 nmol/L, respectively. The detection sensitivity of the FQ- PCR assay was determined to be as low as five copies of the target sequence per reaction using the pGEM-16S rDNA recombinant plasmid as a template, which was 100 times more sensitive than conventional PCR. A standard curve by plotting the threshold cycle values (y) against the common logarithmic copies (logl0n~ as x; n~ is copy number) of pGEM-16S rDNA was generated. The results of intra- and inter-assay variability tests demonstrate that the established FQ-PCR method was highly reproducible. The assay was specific for E. tarda as it showed that there was no cross-reactivity to eight additional bacterial pathogen strains in aquaculture. Thus, the FQ-PCR assay has the potential for diagnostic purposes and for other applications, especially for the rapid detection and quantification of low-grade E. tarda infections. 展开更多
关键词 Edwardsiella tarda TAQMAN real-time PCR detection 16S rDNA
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Real-time RT-PCR Assay for the detection of Tahyna Virus 被引量:2
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作者 LI Hao CAO Yu Xi +6 位作者 HE Xiao Xia FU Shi Hong LYU Zhi HE Ying GAO Xiao Yan LIANG Guo Dong WANG Huan Yu 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第5期374-377,共4页
A real-time RT-PCR (RT-qPCR) assay for the detection of Tahyna virus was developed to monitor Tahyna virus infection in field-collected vector mosquito samples. The targets selected for the assay were S segment sequ... A real-time RT-PCR (RT-qPCR) assay for the detection of Tahyna virus was developed to monitor Tahyna virus infection in field-collected vector mosquito samples. The targets selected for the assay were S segment sequences encoding the nucleocapsid protein from the Tahyna virus. Primers and probes were selected in conserved regions by aligning genetic sequences from various Tahyna virus strains available from GenBank. The sensitivity of the RT-qPCR approach was compared to that of a standard plaque assay in BHK cells. RT-qPCR assay can detect 4.8 PFU of titrated Tahyna virus. Assay specificities were determined by testing a battery of arboviruses, including representative strains of Tahyna virus and other arthropod-borne viruses from China. Seven strains of Tahyna virus were confirmed as positive; the other seven species of arboviruses could not be detected by RT-qPCR. Additionally, the assay was used to detect Tahyna viral RNA in pooled mosquito samples. The RT-qPCR assay detected Tahyna virus in a sensitive, specific, and rapid manner; these findings support the use of the assay in viral surveillance. 展开更多
关键词 PCR real-time RT-PCR Assay for the detection of Tahyna Virus TIME RT
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Real-time RT-PCR Assay for the Detection of Culex flavivirus 被引量:2
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作者 CAO Yu Xi HE Xiao Xia +5 位作者 FU Shi Hong HE Ying LI Hao GAO Xiao Yan LIANG Guo Dong WANG Huan Yu 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第12期917-919,共3页
Based on the Culex flavivirus (CxFV) E gene sequences in GenBank, CxFV-specific primers and probes were designed for real-time reverse transcription-polymerase chain reaction (RT-qPCR). The specificity test revealed t... Based on the Culex flavivirus (CxFV) E gene sequences in GenBank, CxFV-specific primers and probes were designed for real-time reverse transcription-polymerase chain reaction (RT-qPCR). The specificity test revealed that CxFV could be detected using RT-qPCR with the specific CxFV primers and probes; other species of arboviruses were not detected. The stability test demonstrated a coefficient of variation of <1.5%. A quantitative standard curve for CxFV RT-qPCR was established. Quantitative standard curve analysis revealed that the lower detection limit of the RT-qPCR system is 100 copies/mu L. Moreover, RT-qPCR was used to detect CxFV viral RNA in mosquito pool samples. In conclusion, we established a real-time RT-PCR assay for CxFV detection, and this assay is more sensitive and efficient than general RT-PCR. This technology may be used to monitor changes in the environmental virus levels. 展开更多
关键词 PCR real-time RT-PCR Assay for the detection of Culex flavivirus RT time
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Stream-computing of High Accuracy On-board Real-time Cloud Detection for High Resolution Optical Satellite Imagery 被引量:7
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作者 Mi WANG Zhiqi ZHANG +2 位作者 Zhipeng DONG Shuying JIN Hongbo SU 《Journal of Geodesy and Geoinformation Science》 2019年第2期50-59,共10页
This paper focuses on the time efficiency for machine vision and intelligent photogrammetry, especially high accuracy on-board real-time cloud detection method. With the development of technology, the data acquisition... This paper focuses on the time efficiency for machine vision and intelligent photogrammetry, especially high accuracy on-board real-time cloud detection method. With the development of technology, the data acquisition ability is growing continuously and the volume of raw data is increasing explosively. Meanwhile, because of the higher requirement of data accuracy, the computation load is also becoming heavier. This situation makes time efficiency extremely important. Moreover, the cloud cover rate of optical satellite imagery is up to approximately 50%, which is seriously restricting the applications of on-board intelligent photogrammetry services. To meet the on-board cloud detection requirements and offer valid input data to subsequent processing, this paper presents a stream-computing of high accuracy on-board real-time cloud detection solution which follows the “bottom-up” understanding strategy of machine vision and uses multiple embedded GPU with significant potential to be applied on-board. Without external memory, the data parallel pipeline system based on multiple processing modules of this solution could afford the “stream-in, processing, stream-out” real-time stream computing. In experiments, images of GF-2 satellite are used to validate the accuracy and performance of this approach, and the experimental results show that this solution could not only bring up cloud detection accuracy, but also match the on-board real-time processing requirements. 展开更多
关键词 machine VISION intelligent PHOTOGRAMMETRY cloud detection STREAM COMPUTING ON-BOARD real-time processing
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A collision detection approach in virtual environment of micromanipulation robot 被引量:1
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作者 孙立宁 Tan Fusheng Rong Weibin Zhu Jiang 《High Technology Letters》 EI CAS 2005年第4期371-376,共6页
Operators suffer much difficulty in manipulating micro-size objects without the assistance of friendly interfaces due to the scaling effects in micro world. The paper presented a general framework for micromanipulatio... Operators suffer much difficulty in manipulating micro-size objects without the assistance of friendly interfaces due to the scaling effects in micro world. The paper presented a general framework for micromanipulation robot based on virtual reality technology. With the framework we brought forward a FDH (Fixed Direction Hulls) based bounding box method to handle the collision detection of the peg-in-hole microassembly. The collision response model for the collision between micro needle and hole was presented. The virtual force and corresponding displacement were calculated with the model of bending deformation and pressing deformation. Experiments verify the validity of collision response model. 展开更多
关键词 显微操作机器人 虚拟现实 微型组件 碰撞检测
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