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Integrated strategy for real-time wind power fluctuation mitigation and energy storage system control
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作者 Yu Zhang Yongkang Zhang Tiezhou Wu 《Global Energy Interconnection》 EI CSCD 2024年第1期71-81,共11页
To address the impact of wind-power fluctuations on the stability of power systems,we propose a comprehensive approach that integrates multiple strategies and methods to enhance the efficiency and reliability of a sys... To address the impact of wind-power fluctuations on the stability of power systems,we propose a comprehensive approach that integrates multiple strategies and methods to enhance the efficiency and reliability of a system.First,we employ a strategy that restricts long-and short-term power output deviations to smoothen wind power fluctuations in real time.Second,we adopt the sliding window instantaneous complete ensemble empirical mode decomposition with adaptive noise(SW-ICEEMDAN)strategy to achieve real-time decomposition of the energy storage power,facilitating internal power distribution within the hybrid energy storage system.Finally,we introduce a rule-based multi-fuzzy control strategy for the secondary adjustment of the initial power allocation commands for different energy storage components.Through simulation validation,we demonstrate that the proposed comprehensive control strategy can smoothen wind power fluctuations in real time and decompose energy storage power.Compared with traditional empirical mode decomposition(EMD),ensemble empirical mode decomposition(EEMD),and complete ensemble empirical mode decomposition with adaptive noise(CEEMDAN)decomposition strategies,the configuration of the energy storage system under the SW-ICEEMDAN control strategy is more optimal.Additionally,the state-of-charge of energy storage components fluctuates within a reasonable range,enhancing the stability of the power system and ensuring the secure operation of the energy storage system. 展开更多
关键词 SW-ICEEMDAN HESS real-time smoothing Rule-based multi-fuzzy control SoC
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Project⁃based Pollutant Emission Control of Diesel Construction Equipment
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作者 XIE Yixin FAN Hongqin 《施工技术(中英文)》 CAS 2024年第17期85-93,共9页
This paper examines project⁃based policies and regulations implemented globally to control and mitigate emissions from diesel⁃powered construction equipment.This study systematically reviews and analyzes various manag... This paper examines project⁃based policies and regulations implemented globally to control and mitigate emissions from diesel⁃powered construction equipment.This study systematically reviews and analyzes various managerial,regulatory,and technical measures adopted across countries and regions,mostly of advanced economy.Key strategies of control include setting emission thresholds,idling restrictions,perform remote online monitoring,operational time limits,setting low emission zones,and enforced registration systems.The review highlights the rationale,implementation details,and experiences gained from these localized approaches,reduces localized emission sources,improve urban air quality and environmental management efficiency. 展开更多
关键词 equipment diesel emissions control policy analysis
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Real-time localization for underwater equipment using an extremely low frequency electric field 被引量:1
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作者 Jia-wei Zhang Peng Yu +1 位作者 Run-xiang Jiang Tao-tao Xie 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2023年第8期203-212,共10页
A new real-time underwater equipment location method adopting an electric field induced by a standard current source is proposed.Our goals were real-time tracking and location of stationary or moving underwater equipm... A new real-time underwater equipment location method adopting an electric field induced by a standard current source is proposed.Our goals were real-time tracking and location of stationary or moving underwater equipment both in shallow and deep seas,under noisy conditions.The main features of this method are as follows:(1)a standard current source on the water surface,which can be towed by a vehicle,consisting of two electrodes,a signal generator,and a GPS unit;(2)measurement of the extremely low frequency(ELF)electric field emitted by the current source,made possible by electric field sensors on the underwater equipment;(3)position of the underwater equipment is estimated in real time based on a progressive update extended Kalman filter(PUEKF),which is carried out using the propagation model of an ELF electric field because the electric field at the position of the underwater equipment and the current source position are known.We verified the accuracy of our method and confirmed real-time location feasibility through numerical,physical scale,and real-time sea experiments.Through numerical experiments,we verified that our method works for underwater equipment location in real-world conditions,and the location error can be less than 0.2 m.Next,real-time location experiments for stationary underwater measuring equipment in water tank were conducted.The result shows that the location error can be less than 0.1 m.We also confirmed real-time location feasibility through the use of offshore experiment.We expect that our method will complement conventional underwater acoustic location methods for underwater equipment in acoustically noisy environments. 展开更多
关键词 Termsd real-time tracking and location method Underwater equipment location ELF electric field Shallow sea Physical scale experiment Sea experiment
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Real-time parameters monitoring system for underground equipment based on panoramic images
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作者 Chen-guang ZHAO Bo-qiang SHI Zhi-jun HAO Ming-chong XU Chen-tong BIAN 《Journal of Coal Science & Engineering(China)》 2013年第1期109-112,共4页
Complex terrain and working equipment in coal mine underground need a way to ensure coal mine safety. In this paper, the way to monitor the real-time status of underground equipment was put forward, and it was proved ... Complex terrain and working equipment in coal mine underground need a way to ensure coal mine safety. In this paper, the way to monitor the real-time status of underground equipment was put forward, and it was proved to be effective as commanding and dispatching system. Monitoring system for underground equipment based on panoramic images was effectively combined with real-time sensor data and static panoramic images of underground surrounding, which not only realizes real-time status monitoring for underground equipment, but also gets a direct scene for underground surrounding. B/S mode was applied in the monitoring system and this is convenient for users to monitor the equipment. Meantime, it can reduce the waste of the data resource. 展开更多
关键词 panoramic images underground equipment real-time parameters MONITOR B/S mode
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Cold Chain Logistics Equipment Management and Control Based on Internet of Things
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作者 秦立公 吴娇 +1 位作者 董津津 袁媛 《Agricultural Science & Technology》 CAS 2012年第5期1133-1138,共6页
The status and supporting policies of cold chain logistics equipment man- agement and control in China were described. The connotation of Internet of Things and its impact on cold chain logistics equipment management ... The status and supporting policies of cold chain logistics equipment man- agement and control in China were described. The connotation of Internet of Things and its impact on cold chain logistics equipment management and control were ana- lyzed from external form to internal nature. Through introducing the value chain and relevant equipments of cold chain logistics, the correlation between the main technologies in Internet of Things and the common indices for cold chain logistics equipment management and control was analyzed in detail. The application values of Internet of Things technologies in cold chain logistics equipment management and control were illustrated, including the sample analysis on the application of radio-frequency identification (RFID). After the establishment of BSC performance evaluation index system of cold chain logistics equipment management and control, the optimization measures and suggestions on cold chain logistics equipment management and control under Internet of Things were put forward. 展开更多
关键词 Internet of Things Cold chain logistics equipment management and control Radio-frequency identification Balanced scorecard
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Quality control of marine big data——a case study of real-time observation station data in Qingdao 被引量:6
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作者 QIAN Chengcheng LIU Aichao +4 位作者 HUANG Rui LIU Qingrong XU Wenkun ZHONG Shan YU Le 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2019年第6期1983-1993,共11页
Offshore waters provide resources for human beings,while on the other hand,threaten them because of marine disasters.Ocean stations are part of offshore observation networks,and the quality of their data is of great s... Offshore waters provide resources for human beings,while on the other hand,threaten them because of marine disasters.Ocean stations are part of offshore observation networks,and the quality of their data is of great significance for exploiting and protecting the ocean.We used hourly mean wave height,temperature,and pressure real-time observation data taken in the Xiaomaidao station(in Qingdao,China)from June 1,2017,to May 31,2018,to explore the data quality using eight quality control methods,and to discriminate the most effective method for Xiaomaidao station.After using the eight quality control methods,the percentages of the mean wave height,temperature,and pressure data that passed the tests were 89.6%,88.3%,and 98.6%,respectively.With the marine disaster(wave alarm report)data,the values failed in the test mainly due to the influence of aging observation equipment and missing data transmissions.The mean wave height is often affected by dynamic marine disasters,so the continuity test method is not effective.The correlation test with other related parameters would be more useful for the mean wave height. 展开更多
关键词 quality control real-time STATION DATA MARINE BIG DATA Xiaomaidao STATION MARINE DISASTER
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Applying real-time control to enhance the performance of nitrogen removal in CAST system 被引量:10
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作者 WANG Shao-po PENG Yong-zhen +1 位作者 WANG Shu-ying GAO Shou-you 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2005年第5期736-739,共4页
A bench-scale reactor(72 L) red with domestic sewage, was operated more than 3 months with three operation modes: traditional mode, modified mode and real-time control mode, so as to evaluate effects of the operati... A bench-scale reactor(72 L) red with domestic sewage, was operated more than 3 months with three operation modes: traditional mode, modified mode and real-time control mode, so as to evaluate effects of the operation mode on the system performance and to develop a feasible control strategy. Results obtained from fixed-time control study indicate that the variations of the pH and oxidation-reduction potential(ORP) profiles can represent dynamic characteristics of system and the cycle sequences can be controlled and optimized by the control points on the pH and ORP profiles. A control strategy was, therefore, developed and applied to real-time control mode. Compared with traditional mode, the total nitrogen(TN) removal can be increased by approximately 16% in modified mode and a mean TN removal of 92% was achieved in real-time control mode. Moreover, approximately 12.5% aeration energy was saved in real- time control mode. The result of this study shows that the performance of nitrogen removal was enhanced in modified operation mode. Moreover, the real-time control made it possible to optimize process operation and save aeration energy. 展开更多
关键词 cyclic activated sludge technology biological nitrogen removal real-time control oxidation-reduction potential(ORP)
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Control performance comparison between tuned liquid damper and tuned liquid column damper using real-time hybrid simulation 被引量:4
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作者 Zhu Fei Wang Jinting +1 位作者 Jin Feng Lu Liqiao 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2019年第3期695-701,共7页
Tuned liquid damper (TLD) and tuned liquid column damper (TLCD) are two types of passive control devices that are widely used in structural control. In this study, a real-time hybrid simulation (RTHS) technique is emp... Tuned liquid damper (TLD) and tuned liquid column damper (TLCD) are two types of passive control devices that are widely used in structural control. In this study, a real-time hybrid simulation (RTHS) technique is employed to investigate the diff erence in control performance between TLD and TLCD. A series of RTHSs is presented with the premise of the same liquid length, mass ratio, and structural parameters. Herein, TLD and TLCD are physically experimented, and controlled structures are numerically simulated. Then, parametric studies are performed to further evaluate the diff erent performance between TLD and TLCD. Experimental results demonstrate that TLD is more eff ective than TLCD under diff erent amplitude excitations. 展开更多
关键词 tuned LIQUID DAMPER tuned LIQUID COLUMN DAMPER real-time hybrid simulation control effi ciency STRUCTURAL control PARAMETRIC study
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Intercross real-time control strategy in alternating activated sludge process for short-cut biological nitrogen removal treating domestic wastewater 被引量:4
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作者 AKIO TAKIGAWA 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2008年第8期957-963,共7页
To develop technically feasible and economically favorable dynamic process control(DPC)strategies for an alternating activated sludge(AAS)system,a bench-scale continuous-flow alternating aerobic and anoxic reactor,per... To develop technically feasible and economically favorable dynamic process control(DPC)strategies for an alternating activated sludge(AAS)system,a bench-scale continuous-flow alternating aerobic and anoxic reactor,performing short-cut nitrogen removal from real domestic wastewater was operated under different control strategies for more than five months.A fixed-time control(FTC) study showed that bending-points on pH and oxidation-reduction potential(ORP)profiles accurately coincided with the major biologic... 展开更多
关键词 alternating activated sludge process CONTINUOUS-FLOW real-time control NITRIFICATION
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Validation of housekeeping genes as internal controls for studying the gene expression in Pyropia haitanensis(Bangiales, Rhodophyta) by quantitative real-time PCR 被引量:5
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作者 LI Bing CHEN Changsheng +2 位作者 XU Yan JI Dehua XIE Chaotian 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2014年第9期152-159,共8页
Pyropia haitanensis is an economically important mariculture crop in China and has a high research value for several life phenomena, for example environmental tolerance. To explore the mechanisms underlying these char... Pyropia haitanensis is an economically important mariculture crop in China and has a high research value for several life phenomena, for example environmental tolerance. To explore the mechanisms underlying these characteristics, gene expression has been investigated at the whole transcriptome level. Gene expression studies using quantitative real-time PCR should start by selecting an appropriate internal control gene; therefore, the absolute expression abundance of six housekeeping genes (18S rRNA (18S), ubiquitin-conju-ating enzyme (UBC), actin (ACT), β-tubulin (TUB), elongation factors 2 (EF2), and glyceraldehyde-3-phos- phate dehydrogenase (GAPDH) examined by the quantitative real-time PCR in samples corresponding to different strains, life-cycle stages and abiotic stress treatments. Their expression stabilities were assessed by the comparative cycle threshold (Ct) method and by two different software packages: geNorm and NormFinder. The most stable housekeeping gene is UBC and the least stable housekeeping is GADPH. Thus, it is proposed that the most appropriate internal control gene for expression analyses in P. haitanensis is UBC. The results pave the way for further gene expression analyses of different aspects of P. haitanensis biology including different strains, life-history stages and abiotic stress responses. 展开更多
关键词 Pyropia haitanensis quantitative real-time PCR internal control genes gene expression
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Construction Simulation and Real-Time Control for High Arch Dam 被引量:6
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作者 钟登华 任炳昱 吴康新 《Transactions of Tianjin University》 EI CAS 2008年第4期248-253,共6页
A method of combining dynamic simulation with real-time control was proposed to fit the randomness and uncertainty in the high arch dam construction process. The mathematical logic model of high arch dam construction ... A method of combining dynamic simulation with real-time control was proposed to fit the randomness and uncertainty in the high arch dam construction process. The mathematical logic model of high arch dam construction process was established. By combining dynamic construction simulation with schedule analysis, the process of construction schedule forecasting and analysis based on dynamic simulation was studied. The process of real-time schedule control was constructed and some measures for dynamic adjustment and control of construction schedule were provided. A system developed with the method is utilized in a being constructed hydroelectric project located at the Yellow River in northwest China, which can make the pouring plan of the dam in the next stage (a month, quarter or year) to guide the practical construction. The application result shows that the system provides an effective technical support for the construction and management of the dam. 展开更多
关键词 high arch dam construction dynamic simulation schedule forecasting real-time control
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Real-time hybrid simulation for structural control performance assessment 被引量:2
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作者 Juan E Carrion BF Spencer Jr Brian M Phillips 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2009年第4期481-492,共12页
Real-time hybrid simulation is an attractive method to evaluate the response of structures under earthquake loads. The method is a variation of the pseudodynamic testing technique in which the experiment is executed i... Real-time hybrid simulation is an attractive method to evaluate the response of structures under earthquake loads. The method is a variation of the pseudodynamic testing technique in which the experiment is executed in real time, thus allowing investigation of structural systems with rate-dependent components. Real-time hybrid simulation is challenging because it requires performance of all calculations, application of displacements, and acquisition of measured forces, within a very small increment of time. Furthermore, unless appropriate compensation for actuator dynamics is implemented, stability problems are likely to occur during the experiment. This paper presents an approach for real-time hybrid simulation in which compensation for actuator dynamics is implemented using a model-based feedforward compensator. The method is used to evaluate the response of a semi-active control of a structure employing an MR damper. Experimental results show good agreement with the predicted responses, demonstrating the effectiveness of the method for structural control performance assessment. 展开更多
关键词 real-time hybrid simulation MR damper semi-active control actuator dynamics
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Real-Time Iterative Compensation Framework for Precision Mechatronic Motion Control Systems 被引量:2
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作者 Chuxiong Hu Ran Zhou +2 位作者 Ze Wang Yu Zhu Masayoshi Tomizuka 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2022年第7期1218-1232,共15页
With regard to precision/ultra-precision motion systems,it is important to achieve excellent tracking performance for various trajectory tracking tasks even under uncertain external disturbances.In this paper,to overc... With regard to precision/ultra-precision motion systems,it is important to achieve excellent tracking performance for various trajectory tracking tasks even under uncertain external disturbances.In this paper,to overcome the limitation of robustness to trajectory variations and external disturbances in offline feedforward compensation strategies such as iterative learning control(ILC),a novel real-time iterative compensation(RIC)control framework is proposed for precision motion systems without changing the inner closed-loop controller.Specifically,the RIC method can be divided into two parts,i.e.,accurate model prediction and real-time iterative compensation.An accurate prediction model considering lumped disturbances is firstly established to predict tracking errors at future sampling times.In light of predicted errors,a feedforward compensation term is developed to modify the following reference trajectory by real-time iterative calculation.Both the prediction and compen-sation processes are finished in a real-time motion control sampling period.The stability and convergence of the entire control system after real-time iterative compensation is analyzed for different conditions.Various simulation results consistently demonstrate that the proposed RIC framework possesses satisfactory dynamic regulation capability,which contributes to high tracking accuracy comparable to ILC or even better and strong robustness. 展开更多
关键词 Precision motion control prediction model real-time iterative compensation trajectory tracking
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Real-time hybrid simulation of structures equipped with viscoelastic-plastic dampers using a user-programmable computational platform 被引量:2
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作者 Jack Wen Wei Guo Ali Ashasi-Sorkhabi +1 位作者 Oya Mercan Constantin Christopoulos 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2017年第4期693-711,共19页
A user-programmable computational/control platform was developed at the University of Toronto that offers real-time hybrid simulation (RTHS) capabilities. The platform was verified previously using several linear ph... A user-programmable computational/control platform was developed at the University of Toronto that offers real-time hybrid simulation (RTHS) capabilities. The platform was verified previously using several linear physical substructures. The study presented in this paper is focused on further validating the RTHS platform using a nonlinear viscoelastic-plastic damper that has displacement, frequency and temperature-dependent properties. The validation study includes damper component characterization tests, as well as RTHS of a series of single-degree-of-freedom (SDOF) systems equipped with viscoelastic-plastic dampers that represent different structural designs. From the component characterization tests, it was found that for a wide range of excitation frequencies and friction slip loads, the tracking errors are comparable to the errors in RTHS of linear spring systems. The hybrid SDOF results are compared to an independently validated thermal- mechanical viscoelastic model to further validate the ability for the platform to test nonlinear systems. After the validation, as an application study, nonlinear SDOF hybrid tests were used to develop performance spectra to predict the response of structures equipped with damping systems that are more challenging to model analytically. The use of the experimental performance spectra is illustrated by comparing the predicted response to the hybrid test response of 2DOF systems equipped with viscoelastic-plastic dampers. 展开更多
关键词 real-time hybrid simulation user-programmable computational/control platform supplemental dampers performance spectra
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Performance analysis model for real-time Ethernet-based computer numerical control system 被引量:2
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作者 万加富 李迪 +1 位作者 涂钰青 张春华 《Journal of Central South University》 SCIE EI CAS 2011年第5期1545-1553,共9页
In order to optimize the embedded system implementation for Ethernet-based computer numerical control (CNC) system, it is very necessary to establish the performance analysis model and further adopt the codesign met... In order to optimize the embedded system implementation for Ethernet-based computer numerical control (CNC) system, it is very necessary to establish the performance analysis model and further adopt the codesign method from the control, communication and computing perspectives. On the basis of analyzing real-time Ethemet, system architecture, time characteristic parameters of control-loop ere, a performance analysis model for real-time Ethemet-based CNC system was proposed, which is able to include the timing effects caused by the implementation platform in the simulation. The key for establishing the model is accomplished by designing the error analysis module and the controller nodes. Under the restraint of CPU resource and communication bandwidth, the experiment with a case study was conducted, and the results show that if the deadline miss ratio of data packets is 0.2%, then the percentage error is 1.105%. The proposed model can be used at several stages of CNC system development. 展开更多
关键词 computer numerical control (CNC) system real-time Ethemet time characteristic parameters performance analysis model manufacturing accuracy
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A Model Predictive Scheduling Algorithm inReal-Time Control Systems 被引量:2
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作者 Mengya Kang Chenglin Wen Chenxi Wu 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2018年第2期471-478,共8页
Abstract-The ineffective utilization of power resources has attracted much attention in current years. This paper proposes a real-time distributed load scheduling algorithm considering constraints of power supply. Fir... Abstract-The ineffective utilization of power resources has attracted much attention in current years. This paper proposes a real-time distributed load scheduling algorithm considering constraints of power supply. Firstly, an objective function is designed based on the constraint, and a base load forecasting model is established when aggregating renewable generation and non-deferrable load into a power system, which aims to transform the problem of deferrable loads scheduling into a distributed optimal control problem. Then, to optimize the objective function, a real-time scheduling algorithm is presented to solve the proposed control problem. At every time step, the purpose is to minimize the variance of differences between power supply and aggregate load, which can thus ensure the effective utilization of power resources. Finally, simulation examples are provided to illustrate the effectiveness of the proposed algorithm. 展开更多
关键词 Index Terms-Deferrable loads scheduling electric vehicles(EVs) optimal control power supply real-time distributed con-trol.
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An Intelligent Control Method for the Low-Carbon Operation of Energy-Intensive Equipment 被引量:1
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作者 Tianyou Chai Mingyu Li +3 位作者 Zheng Zhou Siyu Cheng Yao Jia Zhiwei Wu 《Engineering》 SCIE EI CAS CSCD 2023年第8期84-95,共12页
Based on an analysis of the operational control behavior of operation experts on energy-intensive equipment,this paper proposes an intelligent control method for low-carbon operation by combining mechanism analysis wi... Based on an analysis of the operational control behavior of operation experts on energy-intensive equipment,this paper proposes an intelligent control method for low-carbon operation by combining mechanism analysis with deep learning,linking control and optimization with prediction,and integrating decision-making with control.This method,which consists of setpoint control,self-optimized tuning,and tracking control,ensures that the energy consumption per tonne is as low as possible,while remaining within the target range.An intelligent control system for low-carbon operation is developed by adopting the end-edge-cloud collaboration technology of the Industrial Internet.The system is successfully applied to a fused magnesium furnace and achieves remarkable results in reducing carbon emissions. 展开更多
关键词 Energy-intensive equipment Low-carbon operation Intelligent control End-edge-cloud collaboration technology
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An Efficient Secure Real-Time Concurrency Control Protocol 被引量:1
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作者 XIAO Yingyuan LIU Yunsheng CHEN Xiangyang 《Wuhan University Journal of Natural Sciences》 CAS 2006年第6期1899-1902,共4页
Secure real-time databases must simultaneously satisfy two requirements in guaranteeing data security and minimizing the missing deadlines ratio of transactions. However, these two requirements can conflict with each ... Secure real-time databases must simultaneously satisfy two requirements in guaranteeing data security and minimizing the missing deadlines ratio of transactions. However, these two requirements can conflict with each other and achieve one requirement is to sacrifice the other. This paper presents a secure real-time concurrency control protocol based on optimistic method. The concurrency control protocol incorporates security constraints in a real-time optimistic concurrency control protocol and makes a suitable tradeoff between security and real-time requirements by introducing secure influence factor and real-time influence factor. The experimental results show the concurrency control protocol achieves data security without degrading real-time perform ance significantly. 展开更多
关键词 real-time databases concurrency control SECURITY covert channel
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Application of predictive control scheduling method to real-time periodic control tasks overrun 被引量:1
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作者 沈青 桂卫华 +1 位作者 阳春华 杨铁军 《Journal of Central South University of Technology》 EI 2007年第2期266-270,共5页
Based on the abort strategy of fixed periods, a novel predictive control scheduling methodology was proposed to efficiently solve overrun problems. By applying the latest control value in the prediction sequences to t... Based on the abort strategy of fixed periods, a novel predictive control scheduling methodology was proposed to efficiently solve overrun problems. By applying the latest control value in the prediction sequences to the control objective, the new strategy was expected to optimize the control system for better performance and yet guarantee the schedulability of all tasks under overrun. The schedulability of the real-time systems with p-period overruns was analyzed, and the corresponding stability criteria was given as well. The simulation results show that the new approach can improve the performance of control system compared to that of conventional abort strategy, it can reduce the overshoot and adjust time as well as ensure the schedulability and stability. 展开更多
关键词 real-time system OVERRUN predictive control scheduling STABILITY
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Identification of potential internal control genes for real-time PCR analysis during stress response in Pyropia haitanensis 被引量:1
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作者 王霞 冯建华 +3 位作者 黄爱优 何林文 牛建峰 王广策 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2017年第6期1432-1441,共10页
Pyropia haitanensis has prominent stress-resistance characteristics and is endemic to China. Studies into the stress responses in these algae could provide valuable information on the stress-response mechanisms in the... Pyropia haitanensis has prominent stress-resistance characteristics and is endemic to China. Studies into the stress responses in these algae could provide valuable information on the stress-response mechanisms in the intertidal Rhodophyta. Here, the effects of salinity and light intensity on the quantum yield of photosystem II in Py. haitanensis were investigated using pulse-amplitude-modulation fluorometry. Total RNA and genomic DNA of the samples under different stress conditions were isolated. By normalizing to the genomic DNA quantity, the RNA content in each sample was evaluated. The cDNA was synthesized and the expression levels of seven potential internal control genes were evaluated using qRT-PCR method. Then, we used geNorm, a common statistical algorithm, to analyze the qRT-PCR data of seven reference genes. Potential genes that may constantly be expressed under different conditions were selected, and these genes showed stable expression levels in samples under a salinity treatment, while tubulin, glyceraldehyde- 3-phosphate dehydrogenase and actin showed stability in samples stressed by strong light. Based on the results of the pulse amplitude-modulation fluorometry, an absolute quantification was performed to obtain gene copy numbers in certain stress-treated samples. The stably expressed genes as determined by the absolute quantification in certain samples conformed to the results of the geNorm screening. Based on the results of the software analysis and absolute quantification, we proposed that elongation factor 3 and 18S ribosomal RNA could be used as internal control genes when the Py. haitanensis blades were subjected to salinity stress, and that a-tubulin and 18S ribosomal RNA could be used as the internal control genes when the stress was from strong light. In general, our findings provide a convenient reference for the selection of internal control genes when designing experiments related to stress responses in Py. haitanensis. 展开更多
关键词 real-time quantitative PCR housekeeping genes internal control genes stress responding Pyropia haitanensis
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