[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,us...[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,using the primers of reference gene actin or ubiquitin.[Result] Actin was more suitable to be the reference gene than ubiquitin.More accurate results were obtained when the 100 ng cDNA template was added at a large volume and a lower concentration.The primer concentration in the range from 0.2 to 0.8 μmol/L we set had no significant influence on the results,so,0.4 μmol/L was selected as the optimal primer concentration in this study.The amplification efficiency was greatly reduced when the annealing temperature was set at 64 ℃,therefore,annealing temperature was set at 60 ℃.Compared with the reaction system of 25 μl,the fluorescence intensity was significantly lower but the CT value did not change greatly in 10 μl system.So,the 10 μl reaction system was selected,which significantly reduces the research costs for the detection of a large amount of samples in future study.展开更多
The controller plays an important rol e in microwave landing system(MLS) navigation equipment.The embedded advanced reduced-instruction-set-computing(RISC) machine(ARM) is introduced to de velop a new MLS controller b...The controller plays an important rol e in microwave landing system(MLS) navigation equipment.The embedded advanced reduced-instruction-set-computing(RISC) machine(ARM) is introduced to de velop a new MLS controller based on the functional requirements.A design includ ing hardware and software of an ARM system is given.The hardware design involve s a ARM smallest system and its interface.μC/OS-II,a good real-time operating system(RTOS),is i ntroduced in the software design.The task partitioning and management are also i nvolved.The experimental results demonstrate that the embedded ARM technique is an effective way in the complex multi-task system,and it meets the high inte gration and real-time processing requirements of the navigation equipment.展开更多
OSEK/VDX(open systems and the corresponding interfaces for automobile electronic/vehicle distributed execu-tive)OS规范描述了一个嵌入式实时操作系统,对系统服务调用的上下文做了严格的限制。违反系统服务调用规范是一种很隐...OSEK/VDX(open systems and the corresponding interfaces for automobile electronic/vehicle distributed execu-tive)OS规范描述了一个嵌入式实时操作系统,对系统服务调用的上下文做了严格的限制。违反系统服务调用规范是一种很隐蔽的错误,不会被编译器发现。提出一种OSEK/VDX OS系统服务调用规范一致性的检测方法,利用森林图描述用户自定义代码中的函数及其调用关系,采用矩阵描述OSEK/VDX OS系统服务调用的规则,遍历该森林图的函数调用路径,对于每条路径,结合该规则矩阵判断其规范一致性。实验和分析表明该方法能有效地检测用户自定义代码中隐藏的违反OSEK/VDX OS系统服务调用规范的错误。展开更多
基金Supported by Guizhou International Cooperation Project on Science and Technology[(2013)7040]the 20th Project of the Joint Committee on Scientific and Technical Cooperation between the Government of the Kingdom of Thailand and the Government of the People’s Republic of China (20-606J)the Fund from Suranaree University of Technology,Thailand (SUT3-304-54-12-29)
文摘[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,using the primers of reference gene actin or ubiquitin.[Result] Actin was more suitable to be the reference gene than ubiquitin.More accurate results were obtained when the 100 ng cDNA template was added at a large volume and a lower concentration.The primer concentration in the range from 0.2 to 0.8 μmol/L we set had no significant influence on the results,so,0.4 μmol/L was selected as the optimal primer concentration in this study.The amplification efficiency was greatly reduced when the annealing temperature was set at 64 ℃,therefore,annealing temperature was set at 60 ℃.Compared with the reaction system of 25 μl,the fluorescence intensity was significantly lower but the CT value did not change greatly in 10 μl system.So,the 10 μl reaction system was selected,which significantly reduces the research costs for the detection of a large amount of samples in future study.
文摘The controller plays an important rol e in microwave landing system(MLS) navigation equipment.The embedded advanced reduced-instruction-set-computing(RISC) machine(ARM) is introduced to de velop a new MLS controller based on the functional requirements.A design includ ing hardware and software of an ARM system is given.The hardware design involve s a ARM smallest system and its interface.μC/OS-II,a good real-time operating system(RTOS),is i ntroduced in the software design.The task partitioning and management are also i nvolved.The experimental results demonstrate that the embedded ARM technique is an effective way in the complex multi-task system,and it meets the high inte gration and real-time processing requirements of the navigation equipment.
文摘OSEK/VDX(open systems and the corresponding interfaces for automobile electronic/vehicle distributed execu-tive)OS规范描述了一个嵌入式实时操作系统,对系统服务调用的上下文做了严格的限制。违反系统服务调用规范是一种很隐蔽的错误,不会被编译器发现。提出一种OSEK/VDX OS系统服务调用规范一致性的检测方法,利用森林图描述用户自定义代码中的函数及其调用关系,采用矩阵描述OSEK/VDX OS系统服务调用的规则,遍历该森林图的函数调用路径,对于每条路径,结合该规则矩阵判断其规范一致性。实验和分析表明该方法能有效地检测用户自定义代码中隐藏的违反OSEK/VDX OS系统服务调用规范的错误。
