The MADS-box gene RhAGL6 plays a master role in regulating the receptacle malformation in rose at low temperature

Low temperature usually results in the developmental deformity of flower organs,immensely affecting the quality of rose flowers.However,it's largely unknown about the regulatory mechanisms activated by low temperature.Here,we used a low temperature-sensitive Rosa hybrida cv.‘Peach Avalanche’to screen a MADS-box gene RhAGL6 via conjoint analysis between RNA sequencing(RNA-seq)and whole-genome bisulfite sequencing(WGBS).Furthermore,we found that low temperature induced the hypermethylation and elevated histone 3 lys-27 trimethylation(H3K27me3)level on the RhAGL6 promoter,leading to decreased RhAGL6 expression.In addition,RhAGL6 silencing resulted in the formation of abnormal receptacles.We also found that the levels of gibberellins(GA3)and abscisic acid(ABA)in the receptacle under low temperature were lower and higher,respectively,than under normal temperature.Promoter activity analysis revealed that GA3 significantly activated RhAGL6 promoter activity,whereas ABA inhibited it.Thus,we propose that RhAGL6 regulates rose receptacle development by integrating epigenetic regulation and phytohormones signaling at low temperature.

Interpretation of GB/T 26779-2021,Hydrogen fuel cell electric vehicles refueling receptacle

With the development of fuel cell electric vehicle industry in China,the 70-MPa hydrogen storage cylinders have been widely applied on vehicles in recent years.The revised standard,GB/T 26779-2021,Hydrogen fuel cell electric vehicle refueling receptacle,was released on March 9,2021 with added stipulations for the 70-MPa hydrogen refuelling receptacle.The main technical contents of GB/T 26779-2021 and its similarities and differences with GB/T 26779-2011 are discussed in this paper.

Platform and receptacle： Musings of modernity arising from Utzon＇s own houses

Utzon throughout his life time designed and built three houses for himself and his family. A study of these houses shows the slow development of a doubt, albeit understated, on his early architectural belief that was much celebrated in his public buildings, such as the Sydney Opera House. But Utzon is not unique in this instance. Some other modern architects too had experienced a similar change, gradual or sudden, in their attitudes towards life and the world, hence the transformation of their architecture as a consequence. This change, on the surface and as represented in architecture, is from a building that boldly embraces outside vista to that of a more internalized receptacle. But unlike Corbusier, Utzon, instead of a complete transformation, showed an inner conflict arising from this doubt, which is between the modern urge to conquer the capacious space out there and a yearning for an interior life.

Intraspecific genetic analysis,gamete release performance,and growth of Sargassum muticum(Fucales,Phaeophyta) from China

Sargassum muticum is one of the most abundant and conspicuous native macroalgae species on the northern coasts of China. It often forms large-scale seaweed beds in subtidal zones. This investigation was designed to understand the intraspecific genetic relationships of this alga based on samples collected from four northern coastal sites of China, and to evaluate gamete release and growth capacity in laboratory conditions. The nuclear internal transcribed spacer 2 （ITS2） sequences of 16 samples from four locations were identical. Based on cox3 gene and partial rbcLS operon sequences, intraspecific genetic variability was detected with three and two ribotypes, respectively. Temperature, not irradiance, was shown to significantly affect gamete release and fertilization. Elevated temperature and irradiance enhanced the growth of germlings and vegetative branchlets. Maximum growth rate of germlings was detected at 18-24℃and an irradiance of 60-100 μmol photons/（m^2.s）. Under ambient conditions （12-25℃ and 60-125 gmol photons/ （m2.s））, relative growth rate of young branchlets could reach 7.5%/d.

Imitations of Beings Enter and Exit： Plotinus on Incorporeal Matter in Plato： Ⅲ 6[26] 11-15

Plotinus＇ account of matter in Ennead III 6[26] 11-15 serves two purposes. The terms, evil and ugly, present the negative side of matter＇s causality, providing for the change characteristic of the sensible world and the possibility of ontological evil and privation as well as of moral evil among human beings. The receptacle and other images from Plato＇s Timaeus present the positive side of this causality, matter as allowing for the presence of forms in the bodies of the sensible world. Plotinus explicitly articulates the linguistic problem surrounding the nature of matter, since language is derived from the corporeal and thus needs constant correction when applied to matter as incorporeal. His use of language, thus, always has two phases, first, capturing the nature of matter as aptly as possible, and second, highlighting the difference between matter and the image, analogy, or metaphor used to help explain it.

Early Development of Silvetia babingtonii(Fucales, Phaeophyceae)

Silvetia babingtonii is a potentially economic brown alga for sources of food and high-value added utilization. So far, sporeling nursery and field cultivation has not been successful. The lack of knowledge on development and life cycle of this alga hinder the development of techniques for the sporeings and cultivation. In this study, internal structure of oogonium and antherium of S. babingtonii was observed with hematoxylin and eosin staining and through microscope. Meanwhile, early development from zygotes to juvenile sporelings was studied at 20℃ under 60–100 μmol photons m-2 s-1. Zygotes germinated and divided into thallus and rhizoid cells. The larger thallus cells further divided and developed into juvenile sporelings; while the smaller rhizoid cells divided and elongated into rhizoid hairs. These findings documented the life cycle of S. babingtonii and provided fundamental knowledge for sporeling nursery in the near future.