Creation and anti-cancer potency in HeLa cells of a novel chimeric toxin, HMGNCIDIN, composed of HMGN2 α- helical domain and PE38 KDEL domain Ⅲ

Using targeted toxins is a promising approach for the therapy of cancer and autoimmune diseases, as well as other disorders/The high mobility group chromosomal protein N2 （HMGN2） is one of the most abundant and well-characterized classes of nonhistone nuclear proteins, which seems to function as architectural elements in chromatin.2 Recently our group isolated an antimicrobial polypeptide from human LAK cells and cervical mucus,

型间嵌合性脊髓灰质炎病毒的组建及抗原性分析

以脊髓灰质炎病毒1型Mahoney株全基因cDNA为载体,将人工合成的2型Laosing株与3型Leon株的中和抗原位点Ⅰ(N-AgⅠ)的cDNA片段,插入Mahoney株N-AgⅠ的相应部位,构成1/2型与1/3型嵌合基因,经体外转染与病毒纯化,得到4株型间嵌合病毒。血清学试验表明,此嵌合病毒呈现多价抗原反应性;嵌合病毒可同时或分别被2个母型毒株的抗血清中和;免疫家兔后能产生2个型的中和抗体。