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An iterative ITI cancellation method for multi-head multi-track bit-patterned magnetic recording systems
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作者 Santi Koonkamkhai Piya Kovintavewat 《Digital Communications and Networks》 SCIE CSCD 2021年第1期107-112,共6页
Bit-Pattemed Magnetic Recording(BPMR)is one of the emerging data storage technologies,which promises an Areal Density(AD)of about 4 Tb/in2.However,a major problem practically encountered in a BPMR system is Inter-Trac... Bit-Pattemed Magnetic Recording(BPMR)is one of the emerging data storage technologies,which promises an Areal Density(AD)of about 4 Tb/in2.However,a major problem practically encountered in a BPMR system is Inter-Track Interference(ITI)that can deteriorate the overall system performance,especially at high ADs.This paper proposes an iterative ITI cancellation method for an m-head m-track BPMR system,which uses m heads to read m adjacent tracks and decodes them simultaneously.To cancel the ITI,we subtract the weighted readback signals of adjacent tracks,acting as the ITI signals,from the readback signal of the target track,before passing the refined readback signal to a turbo decoder.Then,the decoded data will be employed to reconstruct the ITI signal for the next turbo iteration.Experimental results indicate that the proposed system performs better than the conventional system that uses one head to read one data track.Furthermore,we also find out that the proposed system is more robust to media noise and track misregistration than the conventional system. 展开更多
关键词 Emerging magnetic recording technology Multi-head multi-track Inter-track interference Turbo decoder
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FAULT LOCATION AND RECORDING SYSTEM OF TRANSMISSION LINES BASED ON COMPUTER NETWORK 被引量:1
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作者 贺家李 孙雅明 贺继红 《Transactions of Tianjin University》 EI CAS 1997年第1期17-23,共7页
In this paper a fault location and recording system based on a computer network is presented. A brief description of the system structure and main features are given. Emphasis is placed on the accurate fault location ... In this paper a fault location and recording system based on a computer network is presented. A brief description of the system structure and main features are given. Emphasis is placed on the accurate fault location method for extra high voltage and long distance transmission lines. 展开更多
关键词 computer network fault location recording system
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HF-VHF dual-channel multifunctional radio astronomy terminal system
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作者 Kaijing Liu Liang Dong +2 位作者 Huanhuan Xie Baoxin Li Jingzhi Zhou 《Astronomical Techniques and Instruments》 CSCD 2024年第2期140-149,共10页
The high frequency-very high frequency(HF-VHF)frequency band is of significant importance in astronomical observations,with applications studying various phenomena such as space weather,solar radio emissions,planetary... The high frequency-very high frequency(HF-VHF)frequency band is of significant importance in astronomical observations,with applications studying various phenomena such as space weather,solar radio emissions,planetary eruptions in the solar system,pulsars,transient sources,and reionization of the early universe.This article introduces the HF-VHF frequency band multifunctional radio astronomical terminal system based on a dual-channel high-speed acquisition board with a frequency observation range of 1-250 MHz and a sampling rate of 500 Msps(Mega samples per second).The maximum quantization bit of the system is 14 bits,with a maximum time resolution of 0.1 s and a maximum spectral resolution of 16 kHz.The system combines spectral analysis of solar radio signals and recording of time-domain data of signals interfering with long baselines,and adopts a server-client separation mode to allow remote operation with separate permissions.It is used in the China-Malaysia joint astronomy project,which can carry out single-site observation of solar radio signals as well as interferometric observation of signals from multiple sites. 展开更多
关键词 HF-VHF Solar radio High-speed acquisition Frequency domain analysis Time-domain recording.
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A Novel System for Recognizing Recording Devices from Recorded Speech Signals
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作者 Yongqiang Bao Qi Shao +4 位作者 Xuxu Zhang Jiahui Jiang Yue Xie Tingting Liu Weiye Xu 《Computers, Materials & Continua》 SCIE EI 2020年第12期2557-2570,共14页
The field of digital audio forensics aims to detect threats and fraud in audio signals.Contemporary audio forensic techniques use digital signal processing to detect the authenticity of recorded speech,recognize speak... The field of digital audio forensics aims to detect threats and fraud in audio signals.Contemporary audio forensic techniques use digital signal processing to detect the authenticity of recorded speech,recognize speakers,and recognize recording devices.User-generated audio recordings from mobile phones are very helpful in a number of forensic applications.This article proposed a novel method for recognizing recording devices based on recorded audio signals.First,a database of the features of various recording devices was constructed using 32 recording devices(20 mobile phones of different brands and 12 kinds of recording pens)in various environments.Second,the audio features of each recording device,such as the Mel-frequency cepstral coefficients(MFCC),were extracted from the audio signals and used as model inputs.Finally,support vector machines(SVM)with fractional Gaussian kernel were used to recognize the recording devices from their audio features.Experiments demonstrated that the proposed method had a 93.4%accuracy in recognizing recording devices. 展开更多
关键词 recording device recognition Mel-frequency cepstral coefficients support vector machines
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Systematic Recording of Chinese Storytelling Art
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作者 Vibeke Bordahl 《China & The World Cultural Exchange》 2002年第4期36-38,共3页
Chinese Storytelling; Gem of theWorld Oral HeritageAccording to historical records, storytelling,or "Shuoshu", had already become a professionsince the Song Dynasty (960-1279). In theprocess of the increasin... Chinese Storytelling; Gem of theWorld Oral HeritageAccording to historical records, storytelling,or "Shuoshu", had already become a professionsince the Song Dynasty (960-1279). In theprocess of the increasing urbanization ever since,the storyteller was regarded as an integral 展开更多
关键词 St In systematic recording of Chinese Storytelling Art
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A DYNAMIC ECG MONITORING AND RECORDING SYSTEM
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作者 Lijun Tian, Zongzhan Du, Dongqing Wang(Department of Electrical PowerShandong Polytechnic University,Jinan, 250014,China)Aili Wang(Shandong Institute of Traditional Chinese Medicine,Jinan, 250014,China) 《Chinese Journal of Biomedical Engineering(English Edition)》 1994年第1期38-46,共9页
This peper proposes a new type of dynamic ECG (Holier) monitoring andrecording system based on technology or computer controlling and high storage capacitydynamic RAM (DRAM). The system realizes the monitoring and rec... This peper proposes a new type of dynamic ECG (Holier) monitoring andrecording system based on technology or computer controlling and high storage capacitydynamic RAM (DRAM). The system realizes the monitoring and recording ECG signalscontinuously up to 24 hours with the double error criterion ECG data compression algorithm or time error and amplitude error[1], adaptive algorithm for detecting andanalysing the oRS complexes[2] and the technology of high storage capacity DRAM. Itsadvantages are high storage Capacity (4MB), low power dissipation (less titan 30mA). 展开更多
关键词 MONITORING and recording high storage capacity DYNAMIC RAM DATACOMPRESSION detecting and analysing QRS SINGLE-CHIP microcomputer.
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A 3-LEAD 24-HOUR HOLTER SYSTEM BASED ON CASSETTE TAPE DATA RECORDING TECHNOLOGY
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作者 Jiang-ming Li Xin-yu Li Jiang-an Li Institute of Biomedical Engineering,University of Science and Technology of China,Hefei 230026,Anhui,China 《Chinese Journal of Biomedical Engineering(English Edition)》 1993年第4期165-165,共1页
Holter is a kind of widely-used clinical instrument to monitor and diagnoseheart diseases.At present,some kinds of Holter record analogous ECG signal di-rectly.But their complicated recording devices lead to their hig... Holter is a kind of widely-used clinical instrument to monitor and diagnoseheart diseases.At present,some kinds of Holter record analogous ECG signal di-rectly.But their complicated recording devices lead to their high price.Other kindsintroduce semiconductor memory.But their limited capacity makes them quite diffi-cult to record 24-hour ECG signal.The 24-hour Holter system based on digital stor- 展开更多
关键词 HOLTER analogous instrument recording monitor minutes distortion TRANSFERRED
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High repetition granular Co/Pt multilayers with improved perpendicular remanent magnetization for high-density magnetic recording
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作者 李智 张昆 +7 位作者 杜奥 张洪超 陈伟斌 徐宁 郝润润 颜世申 赵巍胜 冷群文 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第2期405-410,共6页
Thanks to the strong perpendicular magnetic anisotropy(PMA), excellent processing compatibility as well as novel spintronic phenomenon, Co/Pt multilayers have been attracting massive attention and widely used in magne... Thanks to the strong perpendicular magnetic anisotropy(PMA), excellent processing compatibility as well as novel spintronic phenomenon, Co/Pt multilayers have been attracting massive attention and widely used in magnetic storage.However, reversed magnetic domains come into being with the increasing layer repetition ‘N’ to reduce magneto-static energy, resulting in the remarkable diminishment of the remanent magnetization(Mr). As a result, the product of Mrand thickness(i.e., the remanent moment-thickness product, Mrt), a key parameter in magnetic recording for reliable data storing and reading, also decreases dramatically. To overcome this issue, we deposit an ultra-thick granular [Co/Pt]80multilayer with a total thickness of 68 nm on granular SiNxbuffer layer. The Mrt value, Mrto saturation magnetization(Ms) ratio as well as out of plane(OOP) coercivity(Hcoop) are high up to 2.97 memu/cm^(2), 67%, and 1940 Oe(1 Oe = 79.5775 A·m^(-1)),respectively, which is remarkably improved compared with that of continuous [Co/Pt]80multilayers. That is because large amounts of grain boundaries in the granular multilayers can efficiently impede the propagation and expansion of reversed magnetic domains, which is verified by experimental investigations and micromagnetic simulation results. The simulation results also indicate that the value of Mrt, Mr/Msratio, and Hcoopcan be further improved through optimizing the granule size, which can be experimentally realized by manipulating the process parameter of SiNxbuffer layer. This work provides an alternative solution for achieving high Mrt value in ultra-thick Co/Pt multilayers, which is of unneglectable potential in applications of high-density magnetic recording. 展开更多
关键词 granular Co/Pt multilayers perpendicular magnetic anisotropy remanent moment-thickness product magnetic recording
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Recording the direct surgeon’s view with an operating microscopic view improves microscopic ophthalmic surgery training
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作者 Masayuki Akimoto Kosei Tomita +1 位作者 Miyo Yoshida Yuki Hama 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第9期1555-1558,共4页
AIM:To establish a recording system with a direct view of the surgeon to supplement video recording under an operating microscope,which lacks information on the movement and position of the surgeon’s hands,and to fac... AIM:To establish a recording system with a direct view of the surgeon to supplement video recording under an operating microscope,which lacks information on the movement and position of the surgeon’s hands,and to facilitate the reproduction of a skilled surgeon’s technique by a surgeon in training.METHODS:A small camera was attached to the operating microscope with a custom adapter.Microscopic surgeon’s view and direct surgeon’s view through this new camera were recorded in the surgical recording system.Both movies were synchronized and analyzed how do surgeons handle the instruments.RESULTS:A small camera attached to the operating microscope allowed the surgeon’s hands motion to be recorded without interfering with the surgeon’s movements.Different surgeons used different methods to manipulate the ultrasound handpiece and the irrigation/aspiration device.Even in the simple paracentesis procedure,different surgeons used different methods.Surgeons-in-training were able to identify and improve their weaknesses by watching synchronized movies of their hand motions and microscopic view.CONCLUSION:Simultaneous recording the surgical field out of the operating microscopic view by a small camera set on the microscope is comprehensive and improves surgeons-in-training understanding and learning surgeries. 展开更多
关键词 operating microscopic view direct surgeon’s view video recording microscopic surgery training
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An Automatic Threshold Selection Using ALO for Healthcare Duplicate Record Detection with Reciprocal Neuro-Fuzzy Inference System
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作者 Ala Saleh Alluhaidan Pushparaj +4 位作者 Anitha Subbappa Ved Prakash Mishra P.V.Chandrika Anurika Vaish Sarthak Sengupta 《Computers, Materials & Continua》 SCIE EI 2023年第3期5821-5836,共16页
ESystems based on EHRs(Electronic health records)have been in use for many years and their amplified realizations have been felt recently.They still have been pioneering collections of massive volumes of health data.D... ESystems based on EHRs(Electronic health records)have been in use for many years and their amplified realizations have been felt recently.They still have been pioneering collections of massive volumes of health data.Duplicate detections involve discovering records referring to the same practical components,indicating tasks,which are generally dependent on several input parameters that experts yield.Record linkage specifies the issue of finding identical records across various data sources.The similarity existing between two records is characterized based on domain-based similarity functions over different features.De-duplication of one dataset or the linkage of multiple data sets has become a highly significant operation in the data processing stages of different data mining programmes.The objective is to match all the records associated with the same entity.Various measures have been in use for representing the quality and complexity about data linkage algorithms,and many other novel metrics have been introduced.An outline of the problem existing in themeasurement of data linkage and de-duplication quality and complexity is presented.This article focuses on the reprocessing of health data that is horizontally divided among data custodians,with the purpose of custodians giving similar features to sets of patients.The first step in this technique is about an automatic selection of training examples with superior quality from the compared record pairs and the second step involves training the reciprocal neuro-fuzzy inference system(RANFIS)classifier.Using the Optimal Threshold classifier,it is presumed that there is information about the original match status for all compared record pairs(i.e.,Ant Lion Optimization),and therefore an optimal threshold can be computed based on the respective RANFIS.Febrl,Clinical Decision(CD),and Cork Open Research Archive(CORA)data repository help analyze the proposed method with evaluated benchmarks with current techniques. 展开更多
关键词 Duplicate detection healthcare record linkage dataset pre-processing reciprocal neuro-fuzzy inference system and ant lion optimization fuzzy system
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Medi-Block Record Secure Data Sharing in Healthcare System:Issues,Solutions and Challenges
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作者 Zuriati Ahmad Zukarnain Amgad Muneer +1 位作者 Nur Atirah Mohamad Nassir Akram A。Almohammedi 《Computer Systems Science & Engineering》 SCIE EI 2023年第12期2725-2740,共16页
With the advancements in the era of artificial intelligence,blockchain,cloud computing,and big data,there is a need for secure,decentralized medical record storage and retrieval systems.While cloud storage solves stor... With the advancements in the era of artificial intelligence,blockchain,cloud computing,and big data,there is a need for secure,decentralized medical record storage and retrieval systems.While cloud storage solves storage issues,it is challenging to realize secure sharing of records over the network.Medi-block record in the healthcare system has brought a new digitalization method for patients’medical records.This centralized technology provides a symmetrical process between the hospital and doctors when patients urgently need to go to a different or nearby hospital.It enables electronic medical records to be available with the correct authentication and restricts access to medical data retrieval.Medi-block record is the consumer-centered healthcare data system that brings reliable and transparent datasets for the medical record.This study presents an extensive review of proposed solutions aiming to protect the privacy and integrity of medical data by securing data sharing for Medi-block records.It also aims to propose a comprehensive investigation of the recent advances in different methods of securing data sharing,such as using Blockchain technology,Access Control,Privacy-Preserving,Proxy Re-Encryption,and Service-On-Chain approach.Finally,we highlight the open issues and identify the challenges regarding secure data sharing for Medi-block records in the healthcare systems. 展开更多
关键词 Medi-block record healthcare system Blockchain technology secure data sharing
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Ensuring Information Security in Electronic Health Record System Using Cryptography and Cuckoo Search Algorithm
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作者 Arkan Kh Shakr Sabonchi Zainab Hashim Obaid 《Journal of Information Hiding and Privacy Protection》 2023年第1期1-18,共18页
In the contemporary era,the abundant availability of health information through internet and mobile technology raises concerns.Safeguarding and maintaining the confidentiality of patients’medical data becomes paramou... In the contemporary era,the abundant availability of health information through internet and mobile technology raises concerns.Safeguarding and maintaining the confidentiality of patients’medical data becomes paramount when sharing such information with authorized healthcare providers.Although electronic patient records and the internet have facilitated the exchange of medical information among healthcare providers,concerns persist regarding the security of the data.The security of Electronic Health Record Systems(EHRS)can be improved by employing the Cuckoo Search Algorithm(CS),the SHA-256 algorithm,and the Elliptic Curve Cryptography(ECC),as proposed in this study.The suggested approach involves usingCS to generate the ECCprivate key,thereby enhancing the security of data storage in EHR.The study evaluates the proposed design by comparing encoding and decoding times with alternative techniques like ECC-GA-SHA-256.The research findings indicate that the proposed design achieves faster encoding and decoding times,completing 125 and 175 iterations,respectively.Furthermore,the proposed design surpasses other encoding techniques by exhibiting encoding and decoding times that are more than 15.17%faster.These results imply that the proposed design can significantly enhance the security and performance of EHRs.Through the utilization of CS,SHA-256,and ECC,this study presents promising methods for addressing the security challenges associated with EHRs. 展开更多
关键词 Information security electronic health record system CRYPTOGRAPHY cuckoo search algorithms
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Isolation of Protoplast and Ion Channel Recording in Plasma Membrane of Suspension Cells of Populus euphratica 被引量:4
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作者 陈少良 戴松香 +3 位作者 李金克 王沙生 Andrea Polle Aloys Hüttermann 《Forestry Studies in China》 CAS 2002年第1期1-4,共4页
The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “o... The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species. 展开更多
关键词 Populus euphratica suspension cells PROTOPLASTS patch\|clamp cell\|attached recording technique single ion channel
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Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma(DBA/2J)mice 被引量:1
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作者 Yin Yang Zhaoxi Yang +9 位作者 Maoxia Lv Ang Jia Junjun Li Baitao Liao Jing’an Chen Zhengzheng Wu Yi Shi Yang Xia Dezhong Yao Ke Chen 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第1期220-225,共6页
Glaucoma is a leading cause of irreve rsible blindness wo rldwide,and previous studies have shown that,in addition to affecting the eyes,it also causes abnormalities in the brain.However,it is not yet clear how the pr... Glaucoma is a leading cause of irreve rsible blindness wo rldwide,and previous studies have shown that,in addition to affecting the eyes,it also causes abnormalities in the brain.However,it is not yet clear how the primary visual cortex(V1)is altered in glaucoma.This study used DBA/2J mice as a model for spontaneous secondary glaucoma.The aim of the study was to compare the electrophysiological and histomorphological chara cteristics of neurons in the V1between 9-month-old DBA/2J mice and age-matched C57BL/6J mice.We conducted single-unit recordings in the V1 of light-anesthetized mice to measure the visually induced responses,including single-unit spiking and gamma band oscillations.The morphology of layerⅡ/Ⅲneurons was determined by neuronal nuclear antigen staining and Nissl staining of brain tissue sections.Eighty-seven neurons from eight DBA/2J mice and eighty-one neurons from eight C57BL/6J mice were examined.Compared with the C57BL/6J group,V1 neurons in the DBA/2J group exhibited weaker visual tuning and impaired spatial summation.Moreove r,fewer neuro ns were observed in the V1 of DBA/2J mice compared with C57BL/6J mice.These findings suggest that DBA/2J mice have fewer neurons in the VI compared with C57BL/6J mice,and that these neurons have impaired visual tuning.Our findings provide a better understanding of the pathological changes that occur in V1 neuron function and morphology in the DBA/2J mouse model.This study might offer some innovative perspectives regarding the treatment of glaucoma. 展开更多
关键词 DBA/2J DEGENERATION gamma band oscillations GLAUCOMA primary visual cortex(V1) RETINA single-unit recording tuning curve
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Detection of Turbulence Anomalies Using a Symbolic Classifier Algorithm in Airborne Quick Access Record(QAR)Data Analysis 被引量:1
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作者 Zibo ZHUANG Kunyun LIN +1 位作者 Hongying ZHANG Pak-Wai CHAN 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2024年第7期1438-1449,共12页
As the risks associated with air turbulence are intensified by climate change and the growth of the aviation industry,it has become imperative to monitor and mitigate these threats to ensure civil aviation safety.The ... As the risks associated with air turbulence are intensified by climate change and the growth of the aviation industry,it has become imperative to monitor and mitigate these threats to ensure civil aviation safety.The eddy dissipation rate(EDR)has been established as the standard metric for quantifying turbulence in civil aviation.This study aims to explore a universally applicable symbolic classification approach based on genetic programming to detect turbulence anomalies using quick access recorder(QAR)data.The detection of atmospheric turbulence is approached as an anomaly detection problem.Comparative evaluations demonstrate that this approach performs on par with direct EDR calculation methods in identifying turbulence events.Moreover,comparisons with alternative machine learning techniques indicate that the proposed technique is the optimal methodology currently available.In summary,the use of symbolic classification via genetic programming enables accurate turbulence detection from QAR data,comparable to that with established EDR approaches and surpassing that achieved with machine learning algorithms.This finding highlights the potential of integrating symbolic classifiers into turbulence monitoring systems to enhance civil aviation safety amidst rising environmental and operational hazards. 展开更多
关键词 turbulence detection symbolic classifier quick access recorder data
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Delivery of luminescent particles to plants for information encoding and storage
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作者 Wei Li Junjie Lin +7 位作者 Wanyi Huang Qingrou Wang Haoran Zhang Xuejie Zhang Jianle Zhuang Yingliang Liu Songnan Qu Bingfu Lei 《Light(Science & Applications)》 SCIE EI CSCD 2024年第10期2288-2299,共12页
In the era of smart agriculture,the precise labeling and recording of growth information in plants pose challenges for modern agricultural production.This study introduces strontium aluminate particles coated with H_(... In the era of smart agriculture,the precise labeling and recording of growth information in plants pose challenges for modern agricultural production.This study introduces strontium aluminate particles coated with H_(3)PO_(4)as luminescent labels capable of spatial embedding within plants for information encoding and storage during growth.The encapsulation with H_(3)PO_(4)imparts stability and enhanced luminescence to SrAl_(2)O_(4):Eu^(2+),Dy^(3+)(SAO).Using SAO@H_(3)PO_(4)as a low-damage luminescent label,we implement its delivery into plants through microneedles(MNs)patches.The embedded SAO@H_(3)PO_(4)within plants exhibits sustained and unaltered high signal-to-noise afterglow emission,with luminous intensity remaining at approximately 78%of the original for 27 days.To cater to diverse information recording needs,MNs of various geometric shapes are designed for loading SAO@H3PO4,and the luminescent signals in different shapes can be accurately identified through a designed program,the corresponding information can be conveniently viewed on a computer.Additionally,inspired by binary information concepts,MNs patches with specific arrangements of luminescent and non-luminescent points are created,resulting in varied luminescent MNs arrays on leaves.An advanced camera system with a tailored program accurately identifies and maps the labels to the corresponding recorded information.These findings showcase the potential of low-damage luminescent labels within plants,paving the way for convenient and widespread storage of plant growth information. 展开更多
关键词 recording DAMAGE LUMINOUS
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Microelectrode array for bioelectrical signal stimulation and recording
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作者 潘海仙 吕晓迎 +3 位作者 王志功 方涛 邱雷 黄宗浩 《Journal of Southeast University(English Edition)》 EI CAS 2011年第4期361-366,共6页
A microelectrode array(MEA) is presented, which is composed of 60 independent electrodes with 59 working ones and one reference one, and they are divided into 30 pairs. Except for the reference electrode, each pair ... A microelectrode array(MEA) is presented, which is composed of 60 independent electrodes with 59 working ones and one reference one, and they are divided into 30 pairs. Except for the reference electrode, each pair consists of one stimulating electrode and one recording electrode. Supported by the peripheral circuits, four electrode states to study the bioelectrical signal of biological tissue or slice cultured in-vitro on the surface of the electrodes can be realized through each pair of electrodes. The four electrode states are stimulation, recording, stimulation and recording simultaneously, and isolation. The state of each pair of working electrodes can be arbitrarily controlled according to actual needs. The MEAs are fabricated in printed circuit board (PCB) technology. The total area of the PCB-based MEA is 49 mm × 49 mm. The impedance measurement of MEA is carried out in 0.9% sodium chloride solution at room temperature by means of 2-point measurements with an Agilent LCR meter, and the test signal for the impedance measurement is sinusoidal (AC voltage 50 mV, sweeping frequency 20 Hz to 10 kHz). The electrode impedance is between 200 and 3 kΩ while the frequency is between 500 and 1 000 Hz. The electrode impedance magnitude is inversely proportional to the frequency. Experiments of toad sciatic nerve in-vitro stimulation and recording and signal regeneration between isolated toad sciatic nerves are carried out on the PCB-based MEA. The results show that the MEA can be used for bioelectrical signal stimulation, recording, stimulation and recording simultaneously, and isolation of biological tissues or slices in-vitro. 展开更多
关键词 microelectrode array (MEA) stimulation and recording extracellular recording toad sciatic nerve
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An optimized recording method to characterize biophysical and pharmacological properties of acid-sensing ion channel
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作者 李爱 司文 +2 位作者 胡新武 刘长金 曹晓华 《Neuroscience Bulletin》 SCIE CAS CSCD 2008年第3期160-165,共6页
Objective To re-confirm and characterize the biophysical and pharmacological properties of endogenously expressed human acid-sensing ion channel 1a (hASIC1a) current in HEK293 cells with a modified perfusion methods... Objective To re-confirm and characterize the biophysical and pharmacological properties of endogenously expressed human acid-sensing ion channel 1a (hASIC1a) current in HEK293 cells with a modified perfusion methods. Methods With cell floating method, which is separating the cultured cell from coverslip and putting the cell in front of perfusion tubing, whole cell patch clamp technique was used to record hASICla currents evoked by low pH external solution. Results Using cell floating method, the amplitude of hASICla currents activated by pH 5.0 in HEK293 cells is twice as large as that by the conventional method where the cells remain attached to coverslip. The time to reach peak at two different recording conditions is (21±5) ms and (270±25) ms, respectively. Inactivation time constants are (496±23) ms and (2284±120) ms, respectively. The cell floating method significantly increases the amiloride potency of block on hASIC 1 a [IC50 is (3.4± 1.1 ) μmol/L and (2.4± 0.9) μmol/L, respectively]. Both recording methods have similar pH activation ECs0 (6.6±0.6, 6.6±0.7, respectively). Conclusion ASICs channel activation requires fast exchange of extracellular solution with the different pH values. With cell floating method, the presence of hASIC la current was re-confirmed and the biophysical and pharmacological properties of hASIC la channel in HEK293 cells was precisely characterized. This method could be used to study all ASICs and other ligand-gated channels that require fast extracellular solution exchange. 展开更多
关键词 acid-sensing ion channel patch-clamp recording PH
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Fifty Years of Dedication to Technology Dissemination,New Journey Ahead with Splendid Chapter:To the 50^(th) Anniversary of Petroleum Exploration and Development
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作者 Editorial Office of Petroleum Exploration and Development 《Petroleum Exploration and Development》 SCIE 2024年第1期F0002-F0002,共1页
As the New Year kicks off,it's also time to celebrate the 50^(th) Anniversary of Petroleum Exploration and Development.In the past 50 years,Petroleum Exploration and Development has been endeavoring to disseminati... As the New Year kicks off,it's also time to celebrate the 50^(th) Anniversary of Petroleum Exploration and Development.In the past 50 years,Petroleum Exploration and Development has been endeavoring to disseminating technology,witnessing and recording the glorious history of China's oil industry developing from weak to strong.In the new era,we have been focusing on theoretical innovation and technology advancement in oil and gas exploration and development,taking an edge on the latest development of the industry,continuously improving the quality of our journal,and leading the journal in a way of high quality and with special features. 展开更多
关键词 journal Petroleum recording
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Chrysosplenium fallax Koldaeva(Chrysosplenium),a newly recorded species from China
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作者 Xiang Niyan Chen Xitang +2 位作者 Yang Tiange Liu Xihong Liu Hong 《植物科学学报》 CAS CSCD 北大核心 2024年第4期423-430,共8页
Chrysosplenium fallax Koldaeva,recently discovered and collected in Yanji,Jilin Province,repre-sents a newly recorded species of Saxifragaceae in China.This species,native to the Russian Far East,was first described a... Chrysosplenium fallax Koldaeva,recently discovered and collected in Yanji,Jilin Province,repre-sents a newly recorded species of Saxifragaceae in China.This species,native to the Russian Far East,was first described as a new species in 2021.Based on precise field investigations and specimen examination,we provide a comprehensive description of C.fallax and its seed micromorphology.Phylogenetic analysis based on chloroplast genomes of 45 Chrysosplenium species confirmed the systematic position of C.fallax in Chrysosplenium.Voucher specimens were deposited in the Herbarium of South-Central Minzu University(HSN). 展开更多
关键词 Chrysosplenium fallax Chrysosplenium Newly record PHYLOGENY China
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