In the present study, the effect of electroacupuncture (EA) on immune system was observed in the rat by using micro- whole blood direct immunofluorescence staining assay to detect changes of the peripheral blood T lym...In the present study, the effect of electroacupuncture (EA) on immune system was observed in the rat by using micro- whole blood direct immunofluorescence staining assay to detect changes of the peripheral blood T lymphocyte subgroup and employing red blood cell (RBC) C 3b receptor- yeast rosette test and red blood cell-IC rosette test to analyze erythrocytic immune function. Results showed that after EA of “Zusanli" (ST 36), CD+ 4, RBC-C 3bRR and RBC-ICR in the peripheral blood of the normal rats increased significantly while CD+ 8 had no any considerable changes and a positive correlation between CD+ 4 and RBC-C 3bRR was found. In immunosuppression model rats, the values of CD+ 4 and RBC-C 3bRR were obviously lower than those of the normal control group while CD+ 8 had no any striking changes; but after EA treatment, there were no evident differences between EA group and normal control group in the above-mentioned indexes. There were also no any significant differences between non-acupoint group and normal control group in those indexes. Results suggest that EA of “Zusanli" (ST 36) can raise T cell immune function and RBC adhesion function in both normal rats and immunosuppression model rats, both of which present a positive correlation.展开更多
Summary: The efficiency of cold storage red blood cells (CSRBC) or whole blood at -80 ℃ used in 27 Rh(D) negative patients during surgical operation was reported. The Rh(D) negative patients received the transfusion...Summary: The efficiency of cold storage red blood cells (CSRBC) or whole blood at -80 ℃ used in 27 Rh(D) negative patients during surgical operation was reported. The Rh(D) negative patients received the transfusion of CSRBC or whole blood stored at -80 ℃ for 180 to 360 days. The changes in the indexes, such as blood TB, DB, K +, Na +, BUN, Cr, urine protein (URPO), UOB, Hb, HCT, serum total protein, relative to hemolytic reaction and blood volume before and after transfusion were observed. The results showed that after transfusion of CSRBC or whole blood 27 cases were negative for urine protein and UOB, and the levels of BUN and Cr were normal (P>0.05). Blood TB, DB, Hb, and HCT were increased, while pH, blood K + and blood Na + was normal with the difference being not significant before and after operation (P>0.05). Plasma protein was decreased, but there was no significant difference before and after operation (P>0.05). It was suggested that CSRBC or whole blood at -80 ℃ could be safely infused to the Rh(D) negative patients without side effects during the surgical operation.展开更多
To find a simple, effective method of isolating fetal cells from maternal peripheral blood for prenatal diagnosis, 45 women were studied with their gestation being 6-14 weeks and age 21- 30 years. The fetal cells wer...To find a simple, effective method of isolating fetal cells from maternal peripheral blood for prenatal diagnosis, 45 women were studied with their gestation being 6-14 weeks and age 21- 30 years. The fetal cells were isolated from maternal blood by using discontinuous density gradient centrifugation. Some of the isolated cells were made smear and counted under the microscope; others were used for predicting fetal sex by PCR amplification of Y chromosome specific DYZ1 gene. The major cells in the upper separation interface were lymphocytes and monocytes, with occasionally seen nucleated red blood cells (NRBC); while those in the middle separation interface were neutrocytes, with NRBC scattering. The ratio of NRBC/nucleated cells was 1. 98±0. 28× 10-5. There was no significant difference between the first and second trimester (P>0. 05). The amount of isolated fetal cells was sufficient for prenatal genetic diagnosis. Male pregnancy was correctly predicted in 10 out of 13 cases. It is concluded that the method of discontinuous density gradient centrifugation was of considerable importance in the development of non-invasive prenatal genetic diagnosis.展开更多
BACKGROUND Erythrocyte alloantibodies are mainly produced after immune stimulation,such as blood transfusion,pregnancy,and transplantation,and are the leading causes of severe hemolytic transfusion reactions and diffi...BACKGROUND Erythrocyte alloantibodies are mainly produced after immune stimulation,such as blood transfusion,pregnancy,and transplantation,and are the leading causes of severe hemolytic transfusion reactions and difficulty in blood grouping and matching.Therefore,antibody screening is critical to prevent and improve red cell alloantibodies.Routine tube assay is the primary detection method of antibody screening.Recently,erythrocyte-magnetized technology(EMT)has been increasingly used in clinical practice.This study intends to probe the application and efficacy of the conventional tube and EMT in red blood cell alloantibody titration to provide a reference for clinical blood transfusion.AIM To investigate the application value of conventional tube and EMT in red blood cell alloantibody titration and enhance the safety of blood transfusion practice.METHODS A total of 1298 blood samples were harvested from blood donors at the Department of Blood Transfusion of our hospital from March 2021 to December 2022.A 5 mL blood sample was collected in tubing,which was then cut,and the whole blood was put into a test tube for centrifugation to separate the serum.Different red blood cell blood group antibody titers were simultaneously detected using the tube polybrene test,tube antiglobulin test(AGT),and EMT screening irregular antibody methods to determine the best test method.RESULTS Simultaneous detection was performed through the tube polybrene test,tube AGT and EMT screening irregular antibodies.It was discovered that the EMT screening irregular antibody method could detect all immunoglobulin G(IgG)and immunoglobulin M(IgM)irregular antibodies,and the results of manual tube AGT were satisfactory,but the operation time was lengthy,and the equipment had a large footprint.The EMT screening irregular antibody assay was also conducted to determine its activity against type O Rh(D)red blood cells,and the outcomes were satisfactory.Furthermore,compared to the conventional tube method,the EMT screening irregular antibody method was more cost-effective and had significantly higher detection efficiency.CONCLUSION With a higher detection rate,the EMT screening irregular antibody method can detect both IgG and IgM irregular antibodies faster and more effectively than the conventional tube method.展开更多
Eighty-six senile persons,aged 60 years old or over,were treated by medicinalcake-separated moxibustion and the influence of moxibustion upon red blood cell immunity func-tion and free radical was observed.We have ado...Eighty-six senile persons,aged 60 years old or over,were treated by medicinalcake-separated moxibustion and the influence of moxibustion upon red blood cell immunity func-tion and free radical was observed.We have adopted the principle of tonifying the kidney andstrengthening the spleen,promoting blood flow and removing blood stasis.The tonic points suchas Dazhui(GV 14),Shenshu(BL 23),Guanyuan(CV 4),Shenque(CV 8)and Zhsanli(ST36)were chosen.Besides,it was supplemented with the principle of nourishing blood,tonifyingspleen and replenishing essence,using medicinal cake moxibustion.The moxibustion was givenonce every other day,three moxa-cone on each point,and twenty-four times as a therapeuticcourse.The total moxibustion amount is 264 moxa-cone.Before and after the treatment,wemeasured the red blood cell immunity function(RBC-C<sub>3</sub>b,RBC-IC,RFER,RFIR),the SOD ac-tivity of RBC,and the contents of serum LPo. Results show that in senile person,the RBC-C<sub>3</sub>b rosette formation falls while the展开更多
Objective: To probe into the effect of electroacupuncture (EA)on vascular dementia and red cell immune function in the rat. Methods: 30 SD rats were made into renal hypertension rats(RHR) by clamping the kidney arteri...Objective: To probe into the effect of electroacupuncture (EA)on vascular dementia and red cell immune function in the rat. Methods: 30 SD rats were made into renal hypertension rats(RHR) by clamping the kidney arteries with silver clip. 42 days later, their bilateral common carotid arteries were blocked repeatedly to cause cerebral ischemia. The Hypertension vascular dementia model was then set up. Then they were randomly divided into VD model group, EA groupand medication group (Dihydroergotoxine, DHET), with 10 cases in each group. The therapeutic course was 28 days. The ability of learning and memory was using an obs erved by water maze, and the function of red blood cell immune was detected after treatment. Results: the latecy of the EA group and medication group was shorter than that of model group (P<0.05, P<0.005), and that of EA group was shorter than medication group (P<0.05, P<0.005). EA and medication could increase the RBCC 3b receptor flower circle rate and reduce the RBCIC flower circle rate significantly(P<0.05, P< 0.01). Conclusion: The results indicated that EA therapy could raise the ability of learning and memory and improve the function of red cell immune in VD rats, while the therapeutic effect of EAis better than DHET.展开更多
The aim of the present study was to investigate the expression of toll-like receptors (TLR) 9 in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B and C with different virus copies. Th...The aim of the present study was to investigate the expression of toll-like receptors (TLR) 9 in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B and C with different virus copies. The study group included 90 patients (60 with chronic hepatitis B, and 30 with chronic hepatitis C), and 20 healthy people served as control group. The protein and mRNA levels of TLR9 were detected by using flow cytometry and real-time PCR. The serum viral copies of HBV and HCV were measured in all patients, and the correlation between HBV-DNA copies or HCV-RNA copies and the TLR9 expression was analyzed. Our results demonstrated that HBV or HCV infection led to a decreased expression of TLR9 mRNA and protein compared to the control group (P〈0.05). The TLR9 protein and mRNA levels were negatively correlated with serum viral copies of HBV and HCV (r=-0.632, r=-0.909, P〈0.01). It was concluded that TLR9 mRNA and protein are down-regulated in PBMC of HBV-infected or HCV-infected patients, and they are negatively correlated with serum viral copies and play an important role in detecting viral replication of HBV and HCV.展开更多
Objective To investigate the relationship between erythrocyte immune function and selenium (Se) level. Methods Forty-nine Kashin-Beck patients in endemic area aged 13-16 years were divided into two groups and were ora...Objective To investigate the relationship between erythrocyte immune function and selenium (Se) level. Methods Forty-nine Kashin-Beck patients in endemic area aged 13-16 years were divided into two groups and were orally given either selenized yeast or sodium selenite to provide 200 μ g selenium per day for 12 weeks. Erythrocyte selenium level, glutathione peroxidase activity, the rosette formation rates of red blood cells complement receptor typeⅠ(CR1), the immune function of red blood cells, and circulating immune complexes(CIC) were determined. Results After supplementing with selenium for 12 weeks, erythrocyte selenium level, glutathione peroxidase activity, the rosette formation rates of red blood cells CR1 were significantly increased. But the difference in rosette formation rates of IC and CIC content was not significant between before and after Se supplementation. Conclusion The increase of the immune function of the erythrocyte by selenium-supplement may be one of the effective mechanisms for the prevention of Kashin-Beck disease.展开更多
Immune evasion is a strategy used by pathogenic microbes to evade the host immune system in order to ensure successful propagation. Immune evasion is particularly important for the blood stages of Plasmodium falciparu...Immune evasion is a strategy used by pathogenic microbes to evade the host immune system in order to ensure successful propagation. Immune evasion is particularly important for the blood stages of Plasmodium falciparum, the causative agent of the deadly disease malaria tropica. Because Plasmodium blood stage parasites require human erythrocytes for replication, their ability to evade attack by the human immune system is essential for parasite survival. In order to escape immunity-induced killing, the intraerythrocytic parasites have evolved a variety of evasion mechanisms, including expansion of plasmodial surface proteins, organ-specific sequestration of the infected red blood cells and acquisition of immune-regulatory proteins by the parasite. This review aims to highlight recent advances in the molecular understanding of the immune evasion strategies by P. falciparum, including antigenic variation, surface protein polymorphisms and invasion ligand diversification. The review will further discuss new findings on the regulatory mechanisms applied by P. falciparum to avoid lysis by the human complement as well as killing by immune factors of the mosquito vector.展开更多
目的探讨外周血红细胞分布宽度(red blood cell distribution width,RDW)、系统免疫炎症指数(systemic immune-inflammation index,SⅡ)与重性抑郁障碍(major depressive disorder,MDD)的相关性。方法回顾性分析2020-2022年于我院临床...目的探讨外周血红细胞分布宽度(red blood cell distribution width,RDW)、系统免疫炎症指数(systemic immune-inflammation index,SⅡ)与重性抑郁障碍(major depressive disorder,MDD)的相关性。方法回顾性分析2020-2022年于我院临床心理科住院的176例MDD患者和常规体检的209例非MDD对照者的临床资料。从血液分析结果中,得到RDW、SⅡ、红细胞分布宽度与血小板计数比值(RDW to platelet ratio,RPR)。绘制受试者操作特征(receiver operator characteristic,ROC)曲线以确定RDW区分患者与对照的最佳临界值及曲线下面积(area under the curve,AUC)。结果MDD组患者的RDW[中位数及四分位数:13.20(12.70,13.98)vs.12.80(12.40,13.35)]、SⅡ水平[中位数及四分位数:510.87(350.95,878.12)vs.405.33(313.74,539.92)]高于非MDD组对照者,差异有统计学意义(P<0.05),两组间RPR差异无统计学意义(P>0.05)。多因素logistic回归分析结果显示,调整混杂因素后,RDW与MDD呈正关联(OR=3.086,95%CI:1.926~4.944)。ROC曲线结果显示,RDW区分MDD与非MDD的最佳临界值为12.85,AUC为0.647(95%CI:0.592~0.702;P<0.001)。结论高RDW可能是MDD发生的危险因素,是对MDD诊断有价值的重要参数。展开更多
目的探讨丁苯酞胶囊联合依达拉奉在自发性蛛网膜下腔出血术后脑血管痉挛(cerebral vasospasm,CVS)中的应用价值,分析其对脑脊液红细胞和神经功能的影响。方法回顾性分析2020年2月—2022年2月广东省中山大学附属汕头医院/汕头市中心医院...目的探讨丁苯酞胶囊联合依达拉奉在自发性蛛网膜下腔出血术后脑血管痉挛(cerebral vasospasm,CVS)中的应用价值,分析其对脑脊液红细胞和神经功能的影响。方法回顾性分析2020年2月—2022年2月广东省中山大学附属汕头医院/汕头市中心医院收治的80例蛛网膜下腔出血术后CVS患者,按用药方式分为2组。在综合治疗基础上,对照组(n=40)用依达拉奉治疗,研究组(n=40)联合丁苯酞胶囊治疗。比较2组脑脊液红细胞数值、神经功能及不良反应。结果治疗后7、14 d,2组脑脊液红细胞计数均逐渐降低,其中研究组治疗后7、14 d脑脊液红细胞计数分别为(32.52±3.54)×10^(12)/L、(5.52±1.58)×10^(12)/L,低于对照组的(40.20±8.21)×10^(12)/L、(10.25±1.65)×10^(12)/L,差异有统计学意义(P<0.05);治疗后7、14 d,2组美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)评分均逐渐降低,且研究组更低;Barthel指数均逐渐升高,且研究组更高,差异有统计学意义(P<0.05)。2组不良反应总发生率比较,差异无统计学意义(P>0.05)。结论丁苯酞胶囊联合依达拉奉治疗自发性蛛网膜下腔出血术后CVS对微循环和脑组织灌注有改善作用,利于神经功能恢复,不增加不良反应。展开更多
文摘In the present study, the effect of electroacupuncture (EA) on immune system was observed in the rat by using micro- whole blood direct immunofluorescence staining assay to detect changes of the peripheral blood T lymphocyte subgroup and employing red blood cell (RBC) C 3b receptor- yeast rosette test and red blood cell-IC rosette test to analyze erythrocytic immune function. Results showed that after EA of “Zusanli" (ST 36), CD+ 4, RBC-C 3bRR and RBC-ICR in the peripheral blood of the normal rats increased significantly while CD+ 8 had no any considerable changes and a positive correlation between CD+ 4 and RBC-C 3bRR was found. In immunosuppression model rats, the values of CD+ 4 and RBC-C 3bRR were obviously lower than those of the normal control group while CD+ 8 had no any striking changes; but after EA treatment, there were no evident differences between EA group and normal control group in the above-mentioned indexes. There were also no any significant differences between non-acupoint group and normal control group in those indexes. Results suggest that EA of “Zusanli" (ST 36) can raise T cell immune function and RBC adhesion function in both normal rats and immunosuppression model rats, both of which present a positive correlation.
文摘Summary: The efficiency of cold storage red blood cells (CSRBC) or whole blood at -80 ℃ used in 27 Rh(D) negative patients during surgical operation was reported. The Rh(D) negative patients received the transfusion of CSRBC or whole blood stored at -80 ℃ for 180 to 360 days. The changes in the indexes, such as blood TB, DB, K +, Na +, BUN, Cr, urine protein (URPO), UOB, Hb, HCT, serum total protein, relative to hemolytic reaction and blood volume before and after transfusion were observed. The results showed that after transfusion of CSRBC or whole blood 27 cases were negative for urine protein and UOB, and the levels of BUN and Cr were normal (P>0.05). Blood TB, DB, Hb, and HCT were increased, while pH, blood K + and blood Na + was normal with the difference being not significant before and after operation (P>0.05). Plasma protein was decreased, but there was no significant difference before and after operation (P>0.05). It was suggested that CSRBC or whole blood at -80 ℃ could be safely infused to the Rh(D) negative patients without side effects during the surgical operation.
文摘To find a simple, effective method of isolating fetal cells from maternal peripheral blood for prenatal diagnosis, 45 women were studied with their gestation being 6-14 weeks and age 21- 30 years. The fetal cells were isolated from maternal blood by using discontinuous density gradient centrifugation. Some of the isolated cells were made smear and counted under the microscope; others were used for predicting fetal sex by PCR amplification of Y chromosome specific DYZ1 gene. The major cells in the upper separation interface were lymphocytes and monocytes, with occasionally seen nucleated red blood cells (NRBC); while those in the middle separation interface were neutrocytes, with NRBC scattering. The ratio of NRBC/nucleated cells was 1. 98±0. 28× 10-5. There was no significant difference between the first and second trimester (P>0. 05). The amount of isolated fetal cells was sufficient for prenatal genetic diagnosis. Male pregnancy was correctly predicted in 10 out of 13 cases. It is concluded that the method of discontinuous density gradient centrifugation was of considerable importance in the development of non-invasive prenatal genetic diagnosis.
基金Supported by Project of Shanxi Provincial Health Commission,No.2021144.
文摘BACKGROUND Erythrocyte alloantibodies are mainly produced after immune stimulation,such as blood transfusion,pregnancy,and transplantation,and are the leading causes of severe hemolytic transfusion reactions and difficulty in blood grouping and matching.Therefore,antibody screening is critical to prevent and improve red cell alloantibodies.Routine tube assay is the primary detection method of antibody screening.Recently,erythrocyte-magnetized technology(EMT)has been increasingly used in clinical practice.This study intends to probe the application and efficacy of the conventional tube and EMT in red blood cell alloantibody titration to provide a reference for clinical blood transfusion.AIM To investigate the application value of conventional tube and EMT in red blood cell alloantibody titration and enhance the safety of blood transfusion practice.METHODS A total of 1298 blood samples were harvested from blood donors at the Department of Blood Transfusion of our hospital from March 2021 to December 2022.A 5 mL blood sample was collected in tubing,which was then cut,and the whole blood was put into a test tube for centrifugation to separate the serum.Different red blood cell blood group antibody titers were simultaneously detected using the tube polybrene test,tube antiglobulin test(AGT),and EMT screening irregular antibody methods to determine the best test method.RESULTS Simultaneous detection was performed through the tube polybrene test,tube AGT and EMT screening irregular antibodies.It was discovered that the EMT screening irregular antibody method could detect all immunoglobulin G(IgG)and immunoglobulin M(IgM)irregular antibodies,and the results of manual tube AGT were satisfactory,but the operation time was lengthy,and the equipment had a large footprint.The EMT screening irregular antibody assay was also conducted to determine its activity against type O Rh(D)red blood cells,and the outcomes were satisfactory.Furthermore,compared to the conventional tube method,the EMT screening irregular antibody method was more cost-effective and had significantly higher detection efficiency.CONCLUSION With a higher detection rate,the EMT screening irregular antibody method can detect both IgG and IgM irregular antibodies faster and more effectively than the conventional tube method.
基金The project was supported by the National Natural Science Foundation of China
文摘Eighty-six senile persons,aged 60 years old or over,were treated by medicinalcake-separated moxibustion and the influence of moxibustion upon red blood cell immunity func-tion and free radical was observed.We have adopted the principle of tonifying the kidney andstrengthening the spleen,promoting blood flow and removing blood stasis.The tonic points suchas Dazhui(GV 14),Shenshu(BL 23),Guanyuan(CV 4),Shenque(CV 8)and Zhsanli(ST36)were chosen.Besides,it was supplemented with the principle of nourishing blood,tonifyingspleen and replenishing essence,using medicinal cake moxibustion.The moxibustion was givenonce every other day,three moxa-cone on each point,and twenty-four times as a therapeuticcourse.The total moxibustion amount is 264 moxa-cone.Before and after the treatment,wemeasured the red blood cell immunity function(RBC-C<sub>3</sub>b,RBC-IC,RFER,RFIR),the SOD ac-tivity of RBC,and the contents of serum LPo. Results show that in senile person,the RBC-C<sub>3</sub>b rosette formation falls while the
文摘Objective: To probe into the effect of electroacupuncture (EA)on vascular dementia and red cell immune function in the rat. Methods: 30 SD rats were made into renal hypertension rats(RHR) by clamping the kidney arteries with silver clip. 42 days later, their bilateral common carotid arteries were blocked repeatedly to cause cerebral ischemia. The Hypertension vascular dementia model was then set up. Then they were randomly divided into VD model group, EA groupand medication group (Dihydroergotoxine, DHET), with 10 cases in each group. The therapeutic course was 28 days. The ability of learning and memory was using an obs erved by water maze, and the function of red blood cell immune was detected after treatment. Results: the latecy of the EA group and medication group was shorter than that of model group (P<0.05, P<0.005), and that of EA group was shorter than medication group (P<0.05, P<0.005). EA and medication could increase the RBCC 3b receptor flower circle rate and reduce the RBCIC flower circle rate significantly(P<0.05, P< 0.01). Conclusion: The results indicated that EA therapy could raise the ability of learning and memory and improve the function of red cell immune in VD rats, while the therapeutic effect of EAis better than DHET.
基金supported by a grant from Natural Sciences Foundation of Hubei Province, China (No. 2006ABA139)
文摘The aim of the present study was to investigate the expression of toll-like receptors (TLR) 9 in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B and C with different virus copies. The study group included 90 patients (60 with chronic hepatitis B, and 30 with chronic hepatitis C), and 20 healthy people served as control group. The protein and mRNA levels of TLR9 were detected by using flow cytometry and real-time PCR. The serum viral copies of HBV and HCV were measured in all patients, and the correlation between HBV-DNA copies or HCV-RNA copies and the TLR9 expression was analyzed. Our results demonstrated that HBV or HCV infection led to a decreased expression of TLR9 mRNA and protein compared to the control group (P〈0.05). The TLR9 protein and mRNA levels were negatively correlated with serum viral copies of HBV and HCV (r=-0.632, r=-0.909, P〈0.01). It was concluded that TLR9 mRNA and protein are down-regulated in PBMC of HBV-infected or HCV-infected patients, and they are negatively correlated with serum viral copies and play an important role in detecting viral replication of HBV and HCV.
文摘Objective To investigate the relationship between erythrocyte immune function and selenium (Se) level. Methods Forty-nine Kashin-Beck patients in endemic area aged 13-16 years were divided into two groups and were orally given either selenized yeast or sodium selenite to provide 200 μ g selenium per day for 12 weeks. Erythrocyte selenium level, glutathione peroxidase activity, the rosette formation rates of red blood cells complement receptor typeⅠ(CR1), the immune function of red blood cells, and circulating immune complexes(CIC) were determined. Results After supplementing with selenium for 12 weeks, erythrocyte selenium level, glutathione peroxidase activity, the rosette formation rates of red blood cells CR1 were significantly increased. But the difference in rosette formation rates of IC and CIC content was not significant between before and after Se supplementation. Conclusion The increase of the immune function of the erythrocyte by selenium-supplement may be one of the effective mechanisms for the prevention of Kashin-Beck disease.
文摘Immune evasion is a strategy used by pathogenic microbes to evade the host immune system in order to ensure successful propagation. Immune evasion is particularly important for the blood stages of Plasmodium falciparum, the causative agent of the deadly disease malaria tropica. Because Plasmodium blood stage parasites require human erythrocytes for replication, their ability to evade attack by the human immune system is essential for parasite survival. In order to escape immunity-induced killing, the intraerythrocytic parasites have evolved a variety of evasion mechanisms, including expansion of plasmodial surface proteins, organ-specific sequestration of the infected red blood cells and acquisition of immune-regulatory proteins by the parasite. This review aims to highlight recent advances in the molecular understanding of the immune evasion strategies by P. falciparum, including antigenic variation, surface protein polymorphisms and invasion ligand diversification. The review will further discuss new findings on the regulatory mechanisms applied by P. falciparum to avoid lysis by the human complement as well as killing by immune factors of the mosquito vector.
文摘目的探讨外周血红细胞分布宽度(red blood cell distribution width,RDW)、系统免疫炎症指数(systemic immune-inflammation index,SⅡ)与重性抑郁障碍(major depressive disorder,MDD)的相关性。方法回顾性分析2020-2022年于我院临床心理科住院的176例MDD患者和常规体检的209例非MDD对照者的临床资料。从血液分析结果中,得到RDW、SⅡ、红细胞分布宽度与血小板计数比值(RDW to platelet ratio,RPR)。绘制受试者操作特征(receiver operator characteristic,ROC)曲线以确定RDW区分患者与对照的最佳临界值及曲线下面积(area under the curve,AUC)。结果MDD组患者的RDW[中位数及四分位数:13.20(12.70,13.98)vs.12.80(12.40,13.35)]、SⅡ水平[中位数及四分位数:510.87(350.95,878.12)vs.405.33(313.74,539.92)]高于非MDD组对照者,差异有统计学意义(P<0.05),两组间RPR差异无统计学意义(P>0.05)。多因素logistic回归分析结果显示,调整混杂因素后,RDW与MDD呈正关联(OR=3.086,95%CI:1.926~4.944)。ROC曲线结果显示,RDW区分MDD与非MDD的最佳临界值为12.85,AUC为0.647(95%CI:0.592~0.702;P<0.001)。结论高RDW可能是MDD发生的危险因素,是对MDD诊断有价值的重要参数。
文摘目的探讨丁苯酞胶囊联合依达拉奉在自发性蛛网膜下腔出血术后脑血管痉挛(cerebral vasospasm,CVS)中的应用价值,分析其对脑脊液红细胞和神经功能的影响。方法回顾性分析2020年2月—2022年2月广东省中山大学附属汕头医院/汕头市中心医院收治的80例蛛网膜下腔出血术后CVS患者,按用药方式分为2组。在综合治疗基础上,对照组(n=40)用依达拉奉治疗,研究组(n=40)联合丁苯酞胶囊治疗。比较2组脑脊液红细胞数值、神经功能及不良反应。结果治疗后7、14 d,2组脑脊液红细胞计数均逐渐降低,其中研究组治疗后7、14 d脑脊液红细胞计数分别为(32.52±3.54)×10^(12)/L、(5.52±1.58)×10^(12)/L,低于对照组的(40.20±8.21)×10^(12)/L、(10.25±1.65)×10^(12)/L,差异有统计学意义(P<0.05);治疗后7、14 d,2组美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)评分均逐渐降低,且研究组更低;Barthel指数均逐渐升高,且研究组更高,差异有统计学意义(P<0.05)。2组不良反应总发生率比较,差异无统计学意义(P>0.05)。结论丁苯酞胶囊联合依达拉奉治疗自发性蛛网膜下腔出血术后CVS对微循环和脑组织灌注有改善作用,利于神经功能恢复,不增加不良反应。