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中国品牌形象设计的国际化表达研究 被引量:1
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作者 杨超 Marja Seliger 《设计》 2016年第3期46-47,共2页
对中国品牌形象的国际化设计进行研究。用视觉人种志的研究方法,通过笔者在欧洲市场对中国品牌的形象设计现状进行研究,得出经济全球化、信息全球化的背景下,中国品牌进入全球市场,要实现品牌价值的提升,从而达到"Made in china&qu... 对中国品牌形象的国际化设计进行研究。用视觉人种志的研究方法,通过笔者在欧洲市场对中国品牌的形象设计现状进行研究,得出经济全球化、信息全球化的背景下,中国品牌进入全球市场,要实现品牌价值的提升,从而达到"Made in china"到"Design in china"的目的,在这个过程中,视觉传达设计到了很重要的作用。有效提升中国品牌形象设计的方法是对品牌名称进行翻译和对品牌形象进行Redesign。在当代视觉审美语境下,品牌形象的视觉传达设计应将中国审美与西方审美相融合,最终实现品牌形象的国际化设计。 展开更多
关键词 中国品牌 品牌形象设计 国际化设计 Designed in China REDESIGN
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EFL Learner-Focused Curriculum Redesign,Implementation and Evaluation——a case from China Agricultural University
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作者 王智杰 《海外英语》 2017年第21期56-57,59,共3页
The paper investigates the learner-focused English as a Foreign Langue(EFL) curricular innovation in China Agricultural University(referred to as CAU in the following text) from the perspectives of curricular formulat... The paper investigates the learner-focused English as a Foreign Langue(EFL) curricular innovation in China Agricultural University(referred to as CAU in the following text) from the perspectives of curricular formulation,implementation and evaluation of EFL learning & teaching in the one-year duration from Autumn semester of 2016 to Spring semester of 2017.A discussion of the effects of the curricular restructuring is presented. 展开更多
关键词 ELF learner Curriculum redesign IMPLEMENTATION EVALUATION
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Redesigned Surface Based Machining Strategy and Method in Peripheral Milling of Thin-walled Parts 被引量:6
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作者 JIA Zhenyuan GUO Qiang SUN Yuwen GUO Dongming 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2010年第3期282-287,共6页
Currently, simultaneously ensuring the machining accuracy and efficiency of thin-walled structures especially high performance parts still remains a challenge. Existing compensating methods are mainly focusing on 3-ai... Currently, simultaneously ensuring the machining accuracy and efficiency of thin-walled structures especially high performance parts still remains a challenge. Existing compensating methods are mainly focusing on 3-aixs machining, which sometimes only take one given point as the compensative point at each given cutter location. This paper presents a redesigned surface based machining strategy for peripheral milling of thin-walled parts. Based on an improved cutting force/heat model and finite element method(FEM) simulation environment, a deflection error prediction model, which takes sequence of cutter contact lines as compensation targets, is established. And an iterative algorithm is presented to determine feasible cutter axis positions. The final redesigned surface is subsequently generated by skinning all discrete cutter axis vectors after compensating by using the proposed algorithm. The proposed machining strategy incorporates the thermo-mechanical coupled effect in deflection prediction, and is also validated with flank milling experiment by using five-axis machine tool. At the same time, the deformation error is detected by using three-coordinate measuring machine. Error prediction values and experimental results indicate that they have a good consistency and the proposed approach is able to significantly reduce the dimension error under the same machining conditions compared with conventional methods. The proposed machining strategy has potential in high-efficiency precision machining of thin-walled parts. 展开更多
关键词 redesigned surface tool path part deflection error prediction finite element method
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Research on Opportunity-Driven Redesign Process to Cooperate With Training Innovative Engineers in China 被引量:3
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作者 Run-Hua Tan Ya-Fan Dong +1 位作者 Bo-Jun Yang Peng Zhang 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2018年第4期148-159,共12页
Many design engineers in cross-domain industries have attended training classes of TRIZ to improve their innovative abilities in China. Most of them are successful, but others are not. So the latest target of the trai... Many design engineers in cross-domain industries have attended training classes of TRIZ to improve their innovative abilities in China. Most of them are successful, but others are not. So the latest target of the trainers is to improve the training process used now in industry in China and to make the engineers to understand the basic principles of TRIZ better. Based on the mass-engineer-oriented training model(MEOTM) and mechanical engineers’ design cases, a relationship between managing activities about the opportunities for innovation and the training process is set up. It is shown that the inventive problems come first from opportunity searching for engineers. A training and gate system for evaluation is developed to involve the managing activities of the companies in the training process. Then comparison between the general analogous process and the application of TRIZ is made, which shows the advantages and depth principles of TRIZ for the engineers to apply them confidently. Lastly a new process is formed in which opportunity searching, engineers training, inventive problems identifying and solving,and three redesign paths are connected seamlessly. The research proposes an opportunity-driven redesign path that cooperates the training and opportunity searching, which will be applied in future training classes to make more and more engineers to follow. 展开更多
关键词 TRIZ Mass-engineer oriented training model Redesign path Opportunity-driven Redesign process
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Applied research on reliable increase measures of CNC lathes 被引量:4
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作者 于捷 贾亚洲 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2005年第2期218-220,共3页
According to the reliable data of sixteen CNC lathes, each reliable index of the serial CNC lathes is evaluated before tackling and after tackling. According to the failure analysis, reliable improvement measures of t... According to the reliable data of sixteen CNC lathes, each reliable index of the serial CNC lathes is evaluated before tackling and after tackling. According to the failure analysis, reliable improvement measures of the serial CNC lathes are put forward. 展开更多
关键词 CNC lathes reliability increasement measure evaluation index IMPROVEMENT REDESIGN FAILURE tackling
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Robust simultaneous tracking and stabilization of wheeled mobile robots not satisfying nonholonomic constraint 被引量:5
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作者 祝晓才 董国华 +1 位作者 蔡自兴 胡德文 《Journal of Central South University of Technology》 EI 2007年第4期537-545,共9页
A robust unified controller was proposed for wheeled mobile robots that do not satisfy the ideal rolling without slipping constraint.Practical trajectory tracking and posture stabilization were achieved in a unified f... A robust unified controller was proposed for wheeled mobile robots that do not satisfy the ideal rolling without slipping constraint.Practical trajectory tracking and posture stabilization were achieved in a unified framework.The design procedure was based on the transverse function method and Lyapunov redesign technique.The Lie group was also introduced in the design.The left-invariance property of the nominal model was firstly explored with respect to the standard group operation of the Lie group SE(2).Then,a bounded transverse function was constructed,by which a corresponding smooth embedded submanifold was defined.With the aid of the group operation,a smooth control law was designed,which fulfills practical tracking/stabilization of the nominal system.An additional component was finally constructed to robustify the nominal control law with respect to the slipping disturbance by using the Lyapunov redesign technique.The design procedure can be easily extended to the robot system suffered from general unknown but bounded disturbances.Simulations were provided to demonstrate the effectiveness of the robust unified controller. 展开更多
关键词 wheeled mobile robot robust control Lie group transverse function Lyapunov redesign
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MIA-UNet:Multi-Scale Iterative Aggregation U-Network for Retinal Vessel Segmentation 被引量:2
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作者 Linfang Yu Zhen Qin +1 位作者 Yi Ding Zhiguang Qin 《Computer Modeling in Engineering & Sciences》 SCIE EI 2021年第11期805-828,共24页
As an important part of the new generation of information technology,the Internet of Things(IoT)has been widely concerned and regarded as an enabling technology of the next generation of health care system.The fundus ... As an important part of the new generation of information technology,the Internet of Things(IoT)has been widely concerned and regarded as an enabling technology of the next generation of health care system.The fundus photography equipment is connected to the cloud platform through the IoT,so as to realize the realtime uploading of fundus images and the rapid issuance of diagnostic suggestions by artificial intelligence.At the same time,important security and privacy issues have emerged.The data uploaded to the cloud platform involves more personal attributes,health status and medical application data of patients.Once leaked,abused or improperly disclosed,personal information security will be violated.Therefore,it is important to address the security and privacy issues of massive medical and healthcare equipment connecting to the infrastructure of IoT healthcare and health systems.To meet this challenge,we propose MIA-UNet,a multi-scale iterative aggregation U-network,which aims to achieve accurate and efficient retinal vessel segmentation for ophthalmic auxiliary diagnosis while ensuring that the network has low computational complexity to adapt to mobile terminals.In this way,users do not need to upload the data to the cloud platform,and can analyze and process the fundus images on their own mobile terminals,thus eliminating the leakage of personal information.Specifically,the interconnection between encoder and decoder,as well as the internal connection between decoder subnetworks in classic U-Net are redefined and redesigned.Furthermore,we propose a hybrid loss function to smooth the gradient and deal with the imbalance between foreground and background.Compared with the UNet,the segmentation performance of the proposed network is significantly improved on the premise that the number of parameters is only increased by 2%.When applied to three publicly available datasets:DRIVE,STARE and CHASE DB1,the proposed network achieves the accuracy/F1-score of 96.33%/84.34%,97.12%/83.17%and 97.06%/84.10%,respectively.The experimental results show that the MIA-UNet is superior to the state-of-the-art methods. 展开更多
关键词 Retinal vessel segmentation security and privacy redesigned skip connection feature maps aggregation hybrid loss function
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Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses 被引量:1
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作者 Danlei Liu Zilei Zhang +4 位作者 Qingping Wu Peng Tian Haoran Geng Ting Xu Dapeng Wang 《Engineering》 SCIE EI 2020年第4期442-448,共7页
Human noroviruses(HuNoVs)are major foodborne pathogens that cause nonbacterial acute gastroenteritis worldwide.As the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus,detection ... Human noroviruses(HuNoVs)are major foodborne pathogens that cause nonbacterial acute gastroenteritis worldwide.As the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus,detection is mainly dependent on molecular approaches such as reverse transcription polymerase chain reaction(RT-PCR)and reverse transcription quantitative real-time polymerase chain reaction(RTqPCR).The widely used primers and probes for RT-qPCR were established in the early 2000s.As HuNoVs are highly variant viruses,viral genome mutations result in previously designed primers and/or probes that were perfectly matched working less efficiently over time.In this study,a new duplex RT-qPCR(ND-RT-qPCR)was designed for the detection of genogroup Ⅰ(GⅠ)and genogroup Ⅱ(GⅡ)HuNoVs based on an analysis of viral sequences added in the database after 2010.Using long transcribed viral RNAs,the results demonstrate that the sensitivity of ND-RT-qPCR is as low as one genomic copy for both GⅠ and GⅡ HuNoVs.The performance of ND-RT-qPCR was further evaluated by a comparison with the commonly used Kageyama primer/probe sets for RT-qPCR(Kageyama RT-qPCR)for 23 HuNoV-positive clinical samples.All five GⅠ samples were registered as positive by ND-RT-qPCR,whereas only two samples were registered as positive by Kageyama RT-qPCR.All 18 GⅡ samples were registered as positive by ND-RT-qPCR,while 17 samples were registered as positive by Kageyama RT-qPCR.The sensitivity reflected by the quantification cycle(Cq)value was lower in ND-RT-qPCR than in Kageyama RT-qPCR.Our data suggest that ND-RT-qPCR could be a good fit for the detection of current strains of HuNoVs. 展开更多
关键词 Human noroviruses RT-QPCR REDESIGN Primer Probe DETECTION
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Design of controlled elastic and inelastic structures 被引量:1
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作者 A.M.Reinhorn O.Lavan G.P.Cimellaro 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2009年第4期469-479,共11页
One of the founders of structural control theory and its application in civil engineering, Professor Emeritus Tsu T. Soong, envisioned the development of the integral design of structures protected by active control d... One of the founders of structural control theory and its application in civil engineering, Professor Emeritus Tsu T. Soong, envisioned the development of the integral design of structures protected by active control devices. Most of his disciples and colleagues continuously attempted to develop procedures to achieve such integral control. In his recent papers published jointly with some of the authors of this paper, Professor Soong developed design procedures for the entire structure using a design - redesign procedure applied to elastic systems. Such a procedure was developed as an extension of other work by his disciples. This paper summarizes some recent techniques that use traditional active control algorithms to derive the most suitable (optimal, stable) control force, which could then be implemented with a combination of active, passive and semi-active devices through a simple match or more sophisticated optimal procedures. Alternative design can address the behavior of structures using Liapunov stability criteria. This paper shows a unified procedure which can be applied to both elastic and inelastic structures. Although the implementation does not always preserve the optimal criteria, it is shown that the solutions are effective and practical for design of supplemental damping, stiffness enhancement or softening, and strengthening or weakening. 展开更多
关键词 active control integral control design redesign procedure inelastic structures viscoelastic braces
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The Influence of Residents’ Opinions on the Redesign of Urban Underground Streets: A Case Study from <i>Diyi</i>Underground Street 被引量:1
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作者 Pei Zhang 《Journal of Building Construction and Planning Research》 2018年第4期215-233,共19页
The rapid development of urbanization and rapid growth of urban population have brought about various urban problems, and the development of cities to the ground and underground has become an inevitable choice. Underg... The rapid development of urbanization and rapid growth of urban population have brought about various urban problems, and the development of cities to the ground and underground has become an inevitable choice. Underground street is a kind of underground space types, and it is also an important urban recreational resources and tourism resources. The evaluation of underground street users, especially the residents and tourists, has important reference value for the recreational resources’ redevelopment of underground street. Firstly, this paper reviews the relevant research literature on underground street and Post-Occupancy Evaluation theory, then chooses Diyi Underground Street, Chengdu, China, as the research object, and conducts a questionnaire survey and in-depth interviews on residents’ shopping intention from five dimensions of underground street environment, decoration, commercial form, public facilities and other aspects. Finally, the survey data are analyzed quantitatively. The results show that some influence factors such as air quality, daylighting, decoration style etc. greatly affect the willingness of residents to enter the street. Therefore, residents’ recreational experience should be emphasized during the underground street re-planning according to the above factors. 展开更多
关键词 Visitors’ Opinion Resident Post-Occupancy Evaluation REDESIGN URBAN UNDERGROUND Space RECREATION URBAN UNDERGROUND Street
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Collaborative Supply Chain Management: A Critical Redesigning Factor
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作者 Z M Udin 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第S1期257-258,共2页
In improving the competitiveness of business organi sa tion in the 21st century, minimising cost and increasing productivity are no lon ger factors that could promise the success. The changes in customer trends whic h... In improving the competitiveness of business organi sa tion in the 21st century, minimising cost and increasing productivity are no lon ger factors that could promise the success. The changes in customer trends whic h focusing more on product or service customising, high quality and short delive ry times are additional crucial factors that organisation should be aware of. T hese factors have direct relations on how the management could utilise the capab ility of its supply chain management (SCM). The importance of SCM in organisati on specifically to manufacturer is to play a vital role in managing the flow of material and information along the chain from suppliers to customers. In a trad itional way, SCM is mainly manage by the production department in organisation b ut with the advancement in information technology, resulted the changes in worki ng environment. One of the ways that could be implemented in decision making p rocess in order to improve the effectiveness and efficiency of the decision is t hrough collaborative environment. Due to this situation, there is a need to rede sign the existing SCM in order to optimise its capability and functionality in t he way to ensure the organisation competitiveness is sustainable. A minor or ma jor alignment to SCM business processes should be done in order to streamline th e flow of information, which could also affect the flow of materials. Factors s uch as SCM operational, structural and even its managerial are among the issues that are critical in redesigning its business process. Each of these factors has its own attributes such as lead-times, complexity, frequency and organisationa l setting that could lead in improving organisational competitiveness. In additi on, by identifying these factors, it would help the management to plan and desig n the collaborative SCM that would effectively correspond to the changes in busi ness and customer trends. The intention of the paper is to promote a list of fa ctors and attributes, which are critical in redesigning an existing SCM in order to shift its environment to become collaborative SCM. By utilising these facto rs and attributes, a model for redesigning SCM into collaborative environment is currently developed by the author and will be used in the next stage of his res earch. 展开更多
关键词 SCM A Critical Redesigning Factor Collaborative Supply Chain Management
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Fatigue Life Prediction of Horizontal Press Frame Based on Statistical Probability and Its Redesign
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作者 Wei-Wei Zhang Xiao-Song Wang +1 位作者 Bo Yang Shi-Jian Yuan 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2014年第2期43-49,共7页
Horizontal press as an important part of hydro-forming machine is used to output the horizontal force to keep the high internal pressure during tube hydro-forming. However,the horizontal press frame is usually mounted... Horizontal press as an important part of hydro-forming machine is used to output the horizontal force to keep the high internal pressure during tube hydro-forming. However,the horizontal press frame is usually mounted on the press bed and not pre-stressed. Meanwhile it will be subjected to the reaction force caused by liquid pressure. Stresses are concentrated severely on the assemble region due to deformation,and total fatigue life will decrease. In order to predict the total fatigue life of the frame,the simulations are used firstly to determine to stress concentration region,and then strain gauge measurements are carried out under different loads. Next,the methods of statistical probability are conducted to calculate the fatigue life based on long-term load history. Finally a structure with the considerable longer fatigue life is redesigned. 展开更多
关键词 horizontal press statistical probability fatigue life prediction structure redesign
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Stress Analysis of Ellipsoidal Shell with Inner Guide Structure
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作者 Wei-Wei Zhang Xiao-Song Wang +1 位作者 Meng Chen Shi-Jian Yuan 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2014年第5期96-100,共5页
In order to overcome stress concentration and increase fatigue life of ellipsoidal shells with inner guide structure,the stress analysis for strength check is very important. Owing to the main sectional profile with e... In order to overcome stress concentration and increase fatigue life of ellipsoidal shells with inner guide structure,the stress analysis for strength check is very important. Owing to the main sectional profile with ellipsoidal shape,the stress distribution for perfect ellipsoidal shell is firstly conducted based on the theoretical calculation and strain gauges measurement. The experiment results show that the stresses increase gradually from pole region to equatorial plane,but still within elastic range. Secondly,strain gauge measurement for ellipsoidal shells with inner guide structure is conducted. The results show that stresses are concentrated at the vicinity of bottom plate and beyond elastic range,so the structural redesign is needed. Finally based on the analysis mentioned above,a redesigned structure with local thickening is proposed. Experimental research shows that the stress varies more even after structural redesign and within allowable range. Numerical simulation shows that both the deformation and fatigue life after redesign are acceptable. 展开更多
关键词 Iellipsoidal shell strain gauge measurement REDESIGN fatigue life
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Modular Structure Reengineering:Design and Study on Small Mobile Exhibition Modes
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作者 诸葛阳 雷月 《Journal of Donghua University(English Edition)》 EI CAS 2014年第1期80-82,共3页
Modular structure reengineering is a design approach conforming to the mobile exhibition features in nature and giving full play to the flexibility,convenience,and environmental protection of the mobile exhibition mod... Modular structure reengineering is a design approach conforming to the mobile exhibition features in nature and giving full play to the flexibility,convenience,and environmental protection of the mobile exhibition modes. The objective of this paper on modular reengineering study is to analyze the innovative applications of modular design in small mobile exhibition. Firstly,the modular design in the domestic small mobile exhibition was investigated in this paper,and then the significance of modular design in small mobile exhibition was proposed via combining the design and future orientation of small mobile exhibition modes. The conclusion is that modular multi-functional small mobile exhibition is the inevitable direction of the future development of the exhibition industry,as this design mode solves a series of problems including resources intensification,structure rationalization,and cost reduction in a foreseeable scope to facilitate sufficient utilization and development of the small mobile exhibition modes. 展开更多
关键词 mobile exhibition modular REDESIGN environmental protection
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Inverse Eigenvalue Problems for a Structure with Linear Parameters
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作者 伍良生 杨家华 +3 位作者 魏源迁 门浩 杨庆坤 刘振宇 《Journal of Donghua University(English Edition)》 EI CAS 2005年第1期116-119,共4页
The inverse design method of a dynamic system with linear parameters has been studied. For some specified eigenvalues and eigenvectors, the design parameter vector which is often composed of whole or part of coefficie... The inverse design method of a dynamic system with linear parameters has been studied. For some specified eigenvalues and eigenvectors, the design parameter vector which is often composed of whole or part of coefficients of spring and mass of the system can be obtained and the rigidity and mass matrices of an initially designed structure can be reconstructed through solving linear algebra equations. By using implicit function theorem, the conditions of existence and uniqueness of the solution are also deduced. The theory and method can be used for inverse vibration design of complex structure system. 展开更多
关键词 inverse eigenvalue problems REDESIGN structure.
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Using Rapid Prototyping Data to Enhance a Knowledge-Based Framework for Product Redesign
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作者 Stefano FILIPPI Ilaria CRISTOFOLINI 《Intelligent Information Management》 2010年第1期1-13,共13页
The particular characteristics of Rapid Prototyping technologies, both in terms of constrains and opportunities, often require the reconfiguration of the product model to obtain the best compliance with the product fu... The particular characteristics of Rapid Prototyping technologies, both in terms of constrains and opportunities, often require the reconfiguration of the product model to obtain the best compliance with the product functionalities and performances. Within this field of research, a knowledge-based tool named Design GuideLines Collaborative Framework (DGLs-CF) was developed to support both the designers defining the product consistently with the manufacturing technologies and the manufacturers defining the building setup consistently with the product requirements. Present work is focused on enhancing the DGLs-CF knowledge base and on updating the DGLs-CF knowledge management by using the information gathered on some RP technologies. The added-value of this research is represented by an improvement in the Redesign/Reconfig- uration Package, the final result of the DGLs-CF adoption. This is a list of actions to be performed on the product model and on the process parameters to avoid the limitations of the technology and to exploit at best its opportunities. 展开更多
关键词 rapid PROTOTYPING KNOWLEDGE-BASED ENGINEERING product REDESIGN COLLABORATIVE ENGINEERING
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The Redesign of the Business Model Based on the Structural Perspective
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作者 Ding Ning Song Yingge Zhang Yijin 《Chinese Business Review》 2013年第6期407-413,共7页
At present, whether internally or abroad, whether in the theory or business, there is a lack of a unified understanding about the basic concepts of structural system and theoretical category of enterprise business mod... At present, whether internally or abroad, whether in the theory or business, there is a lack of a unified understanding about the basic concepts of structural system and theoretical category of enterprise business model, and its theoretical analysis framework has not been built up, it is the exploratory phase of the model research in the mass. This study aims to analyze the formation mechanism, action mechanism, and evolution mechanism about the business model from the perspective of structure, and designs the enterprise business model architecture and forms a system, besides, explores approaches and strategies about enterprise carrying out business model innovation, and does beneficial bedding in order to use and improve the theoretical system in the process of the enterprise management practice. The significance of this study is to provide analytical methods and tools for the business model questions in the enterprise management practice. This paper firstly defined the connotation of enterprise business model and summarized the seven characteristics of the corporate business model. On the basis of outlining the enterprise business model design theory, the structural design of the corporate business model is mainly discussed. This paper designed the structure of the business model on modular perspective, proposed four constituent units and nine elements of enterprise business model structure system. Through the design of the enterprise business model structure and analysis of the elements that constitute structure, provide the basis for the transformation of innovative enterprise business model, or both enterprise business models, avoid the lack of elements of the enterprise business model structure and lead to business failure. Finally, the paper concludes with the five core strategies for the implementation of the enterprise business model design. 展开更多
关键词 analysis of methods enterprise business model STRUCTURE REDESIGN
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Business Process Reengineering: a Review
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作者 薛华成 《High Technology Letters》 EI CAS 1998年第2期33-37,共5页
This paper presents a review of some principle components of Business Process Reengineering(BPR) for the purpose of understanding and deploying BPR related issues. The historical roots of BPR, the Critical Success Fa... This paper presents a review of some principle components of Business Process Reengineering(BPR) for the purpose of understanding and deploying BPR related issues. The historical roots of BPR, the Critical Success Factors (CSFs) and enablers of BPR, and the modeling and simulation of BPR are discussed and reviewed. 展开更多
关键词 Business process reengineering Business process redesign(BPR) BPR enablers
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Mutations in FMN binding pocket diminish chromate reduction rates for Gh-ChrR isolated from <i>Gluconacetobacter hansenii</i>
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作者 Janin A. Khaleel Chunhong Gong +3 位作者 Yanfeng Zhang Ruimin Tan Thomas C. Squier Hongjun Jin 《Natural Science》 2013年第6期20-24,共5页
A putative chromate ion binding site was identified proximal to a rigidly bound FMN from electron densities in the crystal structure of the quinone reductase from Gluconacetobacter hansenii (Gh-ChrR) (3s2y.pdb). To cl... A putative chromate ion binding site was identified proximal to a rigidly bound FMN from electron densities in the crystal structure of the quinone reductase from Gluconacetobacter hansenii (Gh-ChrR) (3s2y.pdb). To clarify the location of the chromate binding site, and to understand the role of FMN in the NADPH-dependent reduction of chromate, we have expressed and purified four mutant enzymes involving the site-specific substitution of individual side chains within the FMN binding pocket that form non-covalent bonds with the ribityl phosphate (i.e., S15A and R17A in loop 1 between β1 sheet and α1 helix) or the isoalloxanzine ring (E83A or Y84A in loop 4 between the β3 sheet and α4 helix). Mutations that selectively disrupt hydrogen bonds between either the N3 nitrogen on the isoalloxanzine ring (i.e., E83) or the ribitylphos- phoate (i.e., S15) respectively result in 50% or 70% reductions in catalytic rates of chromate reduction. In comparison, mutations that disrupt π-π ring stacking interactions with the isoal-loxanzine ring (i.e., Y84) or a salt bridge with the ribityl phosphate result in 87% and 97% inhibittion. In all cases there are minimal alterations in chromate binding affinities. Collectively, these results support the hypothesis that chromate binds proximal to FMN, and implicate a structural role for FMN positioning for optimal chromate reduction rates. As side chains proximal to the β3/α4 FMN binding loop 4 contribute to both NADH and metal ion binding, we propose a model in which structural changes around the FMN binding pocket couples to both chromate and NADH binding sites. 展开更多
关键词 Bioremediation CHROMATE Reduction ENZYME Redesign Site-Specific Mutagenesis CHROMATE Reductase FLAVIN MONONUCLEOTIDE Promiscuous ENZYME Activities
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Characteristics of User Redesign Process: A Study of Changes Made by Users in Architect-Designed Housing
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《Journal of Civil Engineering and Architecture》 2012年第4期496-503,共8页
Housing is dynamic. It changes with time. A sample of 60 houses in the Shagari Low Cost housing scheme in Kanra Namoda was studied in order to find out the characteristics of changes made by the users. Physical observ... Housing is dynamic. It changes with time. A sample of 60 houses in the Shagari Low Cost housing scheme in Kanra Namoda was studied in order to find out the characteristics of changes made by the users. Physical observations and questionnaires were used as the major instruments of data collection. The results show that 91% of occupants have made various changes to the original designs without the help of professional designers, and some of the changes have completely transformed the outlook of the houses in locational planning, materials and form. This implies that a post architectural design phase exists, suggesting that the design process can be divided into two phases; the initial design by the architect and the subsequent redesign by the user. The paper presents preliminary findings on the general characteristics of user redesign and concludes that a clear process can be established. The architectural design process can, therefore, be expanded to include the user redesign process in order to reflect the entire lifespan of the building. 展开更多
关键词 Architectural design process CHANGES expanded design process HOUSING user redesign
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