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Studies on Reduced Extent Method for Inhibited Thermokinetics Of Enzyme-Catalyzed Reaction──Inhibition of the laccase-catalyzed oxidation of o-dihydroxybenzene by m-dihydroxybenzene
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作者 Xiong Ya Wu Dingquan on Songsheng (Department of Chemistry, Wuhan University, Wuhan 430072,China)Du Yumin (Department of Enviromental Science, Wuhan University) 《Wuhan University Journal of Natural Sciences》 CAS 1996年第1期97-100,共4页
The thermokinetic reduced extent equations of reversible inhibitions for Michaiels-Menten enzymatic reaction were deduced, and then the criteria for distingushing inhibition type was given and the methods for calculat... The thermokinetic reduced extent equations of reversible inhibitions for Michaiels-Menten enzymatic reaction were deduced, and then the criteria for distingushing inhibition type was given and the methods for calculating kinetic parameters, KM,Ki and Urn were suggested. This theory was applied to inverstigate the inhibited thermokinetics of laccase-catalyzed oxidation of o-dihydroxybenzene by m-dihydroxybenzene. The experimental results show the inhibition belongs to reversible competitive type, KM=6.224×10-3 mol L-1, Ki=2. 363 × 10-2 mol. L-1. 展开更多
关键词 THERMOKINETICS enzyme-catalyzed reaction inhibition reduced extent method LACCASE
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Study of Activation and Inhibition of Certain Metal Ions to Amylase Catalyzed Reaction by Microcalorimetry 被引量:1
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作者 张洪林 于秀芳 +2 位作者 聂毅 刘晓静 张刚 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2003年第11期1466-1469,共4页
With or without activation or inhibition of metal ion,the power-time curves of amylase catalyzed reaction were determined by a 2277 thermal activity monitor (Sweden). The Michaelis constant ( K ),apparent Michaelis co... With or without activation or inhibition of metal ion,the power-time curves of amylase catalyzed reaction were determined by a 2277 thermal activity monitor (Sweden). The Michaelis constant ( K ),apparent Michaelis constant ( K _m),maximum velocity ( v _m) and apparent maximum velocity ( v _ am ) of amylase catalyzed reaction were obtained using thermokinetic theory and reduced extent method. On the basis of data obtained,the following relationships between K _m and concentration of metal ion ( c ) were established: for inhibitor of Ni 2+ K _m=2.9648×10 -3 -1.3912×10 -4 cR =0.9998for inhibitor of Co 2+ K _m=1.0227×10 -3 +8.2676×10 -6 cR =0.9955for activator of Ca 2+ K _m=1.0630×10 -7 c 2-1.8311×10 -6 c +9.3058×10 -6 R =0.9999for activator of Li + K _m=5.6300×10 -8 c 2-1.5329×10 -6 c +1.2662×10 -5 R =0.9999 The K _m- c relationships show a strenuous inhibitory effect for Ni 2+ and a strenuous active effect for Ca 2+ . 展开更多
关键词 MICROCALORIMETRY THERMOKINETICS reduced extent amylase catalyzed reaction ACTIVATION INHIBITION metal ions(Ca2+ Li+ Co2+ Ni2+)
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