An Assessment of Saltwater Intrusion in Coastal Regions of Lagos, Nigeria

This paper explains various factors that contribute to saltwater intrusion, including overexploitation of freshwater resources and climate change as well as the different techniques essential for effective saltwater intrusion management. The impact of saltwater intrusion along coastal regions and its impact on the environment, hydrogeology and groundwater contamination. It suggests potential solutions to mitigate the impact of saltwater intrusion, including effective water management and techniques for managing SWI. The application of A.I (assessment index) serves as a guideline to correctly identify wells with SWI ranging from no intrusion, slight intrusion and strong intrusion. The challenges of saltwater intrusion in Lagos and the salinization of wells were investigated using the hydro-chemical parameters. The study identifies four wells (“AA”, “CMS”, “OBA” and “VIL”) as having high electric conductivities, indicating saline water intrusion, while other wells (“EBM”, “IKJ, and “IKO”) with lower electric conductivities, indicate little or no salt-water intrusion, and “AJ” well shows slight intrusion. The elevation of the wells also played a vital role in the SWI across coastal regions of Lagos. The study recommends continuous monitoring of coastal wells to help sustain and reduce saline intrusion. The findings of the study are important for policymakers, researchers, and practitioners who are interested in addressing the challenges of saltwater intrusion along coastal regions. We assessed the SWI across the eight (8) wells using the Assessment Index to identify wells with SWI. Wells in “CMS” and “VIL” has strong intrusions. A proposed classification system based on specific ion ratios categorizes water quality from good (+) to highly (-) contaminated (refer to Table 4). These findings underscore the need for attention and effective management strategies to address groundwater unsuitability for various purposes.

Factors affecting land dissection density in geomorphological regions of China

Land dissection density(LDD)provides morphological evidence regarding prior intense soil erosion and quantifies the distribution of land dissections.A comprehensive understanding of the potential factors influencing the spatial pattern and value of the LDD is vital in geological disasters,soil erosion,and other related domains.Land dissection phenomena in China affects large areas with different morphological,pedological,and climatic characteristics.Prior studies have focused on the potential factors influencing the LDD at a watershed scale.However,these results are insufficient to reflect the status quo of dissection development and its primary influencing factors on a national scale.LDD’s spatial patterns and the dominant factors at a regional scale in millions of square kilometers remain to be ascertained.This study used the geomorphon-based method and the geographical detector model to quantify the spatial pattern of LDD over China and identify the dominant factors affecting this pattern in China’s six first-order geomorphological regions(GR1~GR6).The results yield the following findings:(1)LDD in China ranges from 0~4.55 km/km^(2),which is larger in central and eastern regions than in other regions of China;(2)dominant factors and their dominant risk subcategories vary with each geomorphological region’s primary internal and external forces;(3)the influence of natural factors is more significant on the large regional scale in millions of square kilometers compared to anthropogenic factors;relief degree of land surface(RDLS)is dominant in GR1,GR2,and GR5;the slope is dominant in GR6,soil type is dominant in GR3 and GR4,and lithology plays a critical role in the dominant interactions of GR3,GR4,and GR6;(4)the interactions between factors on LDD’s spatial pattern have a more significant effect than individual factors.

A phylogenetic approach identifies patterns of beta diversity and floristic subregions of the Qinghai-Tibet Plateau

Patterns of taxonomic and phylogenetic beta diversity and their relationships with environmental correlates can help reveal the origin and evolutionary history of regional biota.The Qinghai-Tibet Plateau(QTP)harbors an exceptionally diverse flora,however,a phylogenetic perspective has rarely been used to investigate its beta diversity and floristic regions.In this study,we used a phylogenetic approach to identify patterns of beta diversity and quantitatively delimit floristic regions on the Qinghai-Tibet Plateau.We also examined the relationships between multifaceted beta diversity,geographical distance,and climatic difference,and evaluated the relative importance of various factors(i.e.,climate,topography and history)in shaping patterns of beta diversity.Sørensen dissimilarity indices indicated that patterns of species turnover among sites dominated the QTP.We also found that patterns of both taxonomic and phylogenetic beta diversity were significantly related to geographical distance and climatic difference.The environmental factors that contributed most to these patterns of beta diversity include annual precipitation,mean annual temperature,climatic gradients and climatic instability.Hierarchical dendrograms of dissimilarity and non-metric multidimensional scaling ordination based on phylogenetic beta diversity data identified ten floristic subregions in the QTP.Our results suggest that the contemporary environment and historical climate changes have filtered species composition among sites and eventually determined beta diversity patterns of plants in the QTP.

Research on the Digital Economy's Impact on China's Economic Growth:Based on the Variations in Urbanization Levels Across China's Eastern,Central,and Western Regions and Sectoral Heterogeneity

This paper uses inter-provincial panel data from 2011 to 2017,a linear regression model,and a threshold model to conduct empirical analyses of the impact of the digital economy on China's overall economic growth and the three main sectors of industry.The paper then investigates the impact and effects the digital economy has had on the economic growth of the three main sectors of industry in China's eastern,central,and western regions.Finally,the paper investigates the most significant differences among the various regions and the threshold effects of urbanization levels on the relationship between the digital economy and economic growth.The findings indicate a significantly positive correlation between the digital economy and regional economic growth.Moreover,geographical factors notably influence this correlation.The digital economy exerts a positive effect on all sectors of industry.It may not substantially impact industrial development in regions with highly developed infrastructure.Regarding the other regions,the digital economy exhibits varying degrees of impact due to the differences in the specific indicators.The conclusion drawn by the threshold model is that the magnitude of the threshold effect correlates with geographic factors.No threshold effect was observed in the eastern region,while the threshold effect occurred in the central region when the urbanization levels for the provinces were below 0.6645.Similarly,the threshold effect was noted in the western region when the urbanization level was below 0.3931.Considering all of this,the study also offers policy recommendations that will help balance the regional development of digital economies,accelerate the digital transformation of traditional industries,enhance digital infrastructure construction,refine the formulation and implementation of data policy,and establish relevant incentive mechanisms.

Chemical composition of walnuts from three regions in China

To reveal the characteristic chemicals of walnuts from different origins, we analyzed fatty acid composition,tocopherols, phytosterols and total phenolic content (TPC) of walnuts from three main producing regions inChina. The results showed that walnuts were rich in polyunsaturated fatty acids, and the ratio of ω-6 to ω-3 fattyacids was close to the recommendation of Chinese Nutrition Society. Moreover, walnuts contain high contents oftocopherols (331.20–414.71 mg/kg), phytosterols (97.17–110.35 mg/100 g) and phenols (38.51–48.08 mg GAE/kg). Significant chemical differences exist among walnuts from three production regions. The highest content ofpolyunsaturated fatty acids was found in walnuts from the northern China, the highest content of tocopherols inwalnuts from southwest China, and the highest contents of phytosterol and TPC in walnuts from northwest China.However, there was no significant difference in the tocopherol, phytosterol and TPC content of walnuts betweenthe Northern China and Northwest China. The above results provide important references for manufacturers andconsumers to select suitable walnut scientifically and reasonably.

Dissecting the key genomic regions underlying high yield potential in common wheat variety‘Kenong 9204’

The foundation parents play key roles in the genetic improvement of both yield potential and end-use quality in wheat.Characterizing the genetic basis that underlies certain beneficial traits in the foundation parents will provide theoretical reference for molecular breeding by a design approach.‘Kenong 9204’(KN9204)is a candidate foundation parent characterized by ideotype,high yield potential,and particularly high nitrogen fertilizer utilization.To better understand the genetic basis of its high yield potential,high throughput whole-genome re-sequencing(10×)was performed on KN9204,its parental lines and its derivatives.A high-resolution genetic composition map of KN9204 was constructed,which showed the parental origin of the favorable genomic segments based on the identification of excellent yield-related quantitative trait loci(QTL)from a bi-parental mapping population.Xiaoyan 693(XY693),a wheat–Thinopyrum ponticum partial amphidiploid,contributed a great deal to the high yield potential of KN9204,and three major stable QTLs from XY693 were fine mapped.The transmissibility of key genomic segments from KN9204 to its derivatives were delineated,indicating that haplotype blocks containing beneficial gene combinations were conserved along with directional selection by breeders.Evidence for selection sweeps in the breeding programs was identified.This study provides a theoretical reference for the breeding of high-yield wheat varieties by a molecular design approach.

Histone H3K27me3 methylation regulates the expression of secreted proteins distributed at fast-evolving regions through transcriptional repression of transposable elements

The fine-tuned expression dynamics of the effector genes are pivotal for the transition from vegetative growth to host colonization of pathogenic filamentous fungi.However,mechanisms underlying the dynamic regulation of these genes remain largely unknown.Here,through comparative transcriptome and chromatin immunoprecipitation sequencing(ChIP-seq)analyses of the methyltransferase PoKmt6 in rice blast fungus Pyricularia oryzae(syn.Magnaporthe oryzae),we found that PoKmt6-mediated H3K27me3 deposition was enriched mainly at fast-evolving regions and contributed to the silencing of a subset of secreted proteins(SP)and transposable element(TE)families during the vegetative growth of P.oryzae.Intriguingly,we observed that a group of SP genes,which were depleted of H3K27me3 modification,could also be silenced via the H3K27me3-mediated repression of the nearby TEs.In conclusion,our results indicate that H3K27me3 modification mediated by PoKmt6 regulates the expression of some SP genes in fast-evolving regions through the suppression of nearby TEs.

Plastic-film-side seeding,as an alternative to traditional film mulching,improves yield stability and income in maize production in semi-arid regions

Planting under plastic-film mulches is widely used in spring maize production in arid-cold regions for water conservation and warming the soil.To ameliorate the associated issues such as plastic-film residues and additional labor during the“seedling release”in spring maize production,we have developed a plastic-film-side seeding(PSS)technology with the supporting machinery.In the semi-arid regions of Northwest China,a 7-year trial demonstrated that PSS increased plant number per hectare by 6547 and maize yield by 1686 kg ha–1compared with the traditional method of seeding under plastic-film mulch(PM).Two-year experiments were conducted in two semi-arid regions to further understand the effects of PSS on three important aspects of production:(i)the moisture and temperature of soil,(ii)maize development,yield output,and water use efficiency(WUE),and(iii)the revenue and plastic-film residuals in comparison with that of flat planting(CK)and PM.Continuous monitoring of the soil status demonstrated that,compared with CK,the PSS treatment significantly increased the temperature and moisture of the 0–20 cm soil in the seeding row at the early stage of maize development,and it also promoted grain yield(at 884–1089 kg ha^(–1))and WUE,achieving a similar effect as the PM treatment.Economically,the labor inputs of PSS were equal to CK,whereas the PM cost an additional 960 CNY ha–1in labor for releasing the seedlings from below the film.Overall,the PSS system increased profits by 5.83%(547 CNY ha^(–1)yr^(–1))and 8.16%(748 CNY ha^(–1)yr^(–1))compared with CK and PM,respectively.Environmentally,PSS achieved a residual film recovery rate of nearly 100%and eliminated 96 to 130 kg ha^(–1)of residual plastic-film in PM in 3–5 years of maize production.Collectively,these results show that PSS is an eco-friendly technique for improving yield stability and incomes for the sustainable production of maize in semi-arid regions.

Selecting proper sites for underground dam construction using Multi-Attribute Utility Theory in arid and semi-arid regions

Although the construction of underground dams is one of the best methods to conserve water resources in arid and semi-arid regions,applying efficient methods for the selection of suitable sites for subsurface dam construction remains a challenge.Due to the costly and time-consuming methods of site selection for underground dam construction,this study aimed to present a new method using geographic information systems techniques and decision-making processes.The exclusionary criteria including fault,slope,hypsometry,land use,soil,stream,geology,and chemical properties of groundwater were selected for site selection of dam construction and inappropriate regions were omitted by integration and scoring layers in ArcGIS based on the Boolean logic.Finally,appropriate sites were prioritized using the Multi-Attribute Utility Theory.According to the results of the utility coefficient,seven sites were selected as the region for underground dam construction based on all criteria and experts’opinions.The site of Nazarabad dam was the best location for underground dam construction with a utility coefficient of 0.7137 followed by sites of Akhavan with a utility coefficient of 0.4633 and Mirshamsi with a utility coefficient of 0.4083.This study proposed a new approach for the construction of the subsurface dam at the proper site and help managers and decision-makers achieve sustainable water resources with limited facilities and capital and avoid wasting national capital.

Characteristics and driving factors of abandoned cultivated land in the hilly regions of southern China:A case study in Longnan,Jiangxi Province

The abandonment of cultivated land in southern China was gradually obvious.This research aims to provide a reference for solving the abandonment of cultivated land in hilly regions and promote rural development in China.We examined Longnan county located in the hilly regions of southern China as an example,where abandoned cultivated land is very common.We analyzed its land use data with a field survey to identify the abandoned cultivated land and geospatial characteristics.From the two aspects of social and natural factors,we analyzed the factors driving cultivated land abandonment with the help of Geodetector.The results showed that in 2019,the total area of the abandoned cultivated land in Longnan county was 4,962.35 hm^(2),covering 39.51% of this region.Among the topographic factors,the abandonment rate is positively correlated with elevation and slope gradient,but not with slope direction.Among the land parcel conditions,the abandonment rate is positively correlated with the access to road network and cultivation distance from settlement.At the county level,the abandonment of cultivated land in study area was affected by multiple factors,among which,the direct factor was the reduction in the labor force,such as the decrease of farming laborers and the increase of female population,which made farming unsustainable.Changes in production factors also promoted transformations in farmers’motivation to engage in production,such as the decrease of grain crops and the increase of cash crops,which was the indirect factor affecting cultivated land abandonment.The development of the rural nonagricultural industry affected farmers’enthusiasm,such as the decrease of farming households,which was the fundamental factor leading to cultivated land abandonment in this area.

Dynamic response characteristics of a circular lined tunnel under anisotropy frost heave of overlying soil at the tunnel portal section in cold regions

The rapid development of traffic engineering in cold regions and its consequent problems need to be considered.In this paper,the dynamic response characteristics of the tunnel portal section in cold regions with harmonic load acting on the lining were studied in the frequency domain.The lining is in close contact with the frozen soil,and there is relative movement between the frozen and unfrozen soil due to the phase change.The analytical solution of the vibration of tunnel portal section caused by the harmonic load acting on the lining was derived under the consideration of the anisotropy frost heave of overlying soil.Based on the continuity conditions and boundary conditions,the undetermined coefficients were obtained,and the analytical solutions for different medium displacements and stresses of the cold-region tunnel system were acquired.The vertical pressure coefficient was equivalently simplified as a variable that could be used to replace the thickness of the overlying soil above the tunnel.The analysis of the parameter model shows that the change of the medium parameters(lining,frozen,and unfrozen soil)affects the circumferential stresses,the radial displacements and their peak frequencies of the soil.For example,the increase of density ratio of tunnel lining to frozen soil decreases the radial stresses of the frozen and unfrozen soil;the increase of volumetric frost heaving strain of the frozen soil increases the radial displacements of the frozen surface and decreases the stability of the frozen surface;the increasing of thickness of the frozen soil significantly reduces the radial displacement of unfrozen soil at dimensionless radius η=4.5 compared with that of frozen soil at η=1.5.

Correlation analysis of longitudinal cracks and vertical deformation within asphalt pavement of cold regions

The asphalt pavement longitudinal crack is a common distress in cold regions,resulting from uneven deformation of the subgrade.Analysis of the correlation law between uneven deformation and crack distress is of positive significance for understanding the mechanism of crack initiation,and putting forward treatment measures.In view of the complexity of longitudinal crack inducement and road surface deformation,the grey relational method was used to analyze this relationship.Through long-term monitoring of the vertical deformation data of typical road sections,the vertical deformation law of the pavement surface and its deformation characteristics under the action of temperature field are analyzed.Parameters such as vertical relative deformation,vertical relative deformation rate and vertical differential deformation VDSr were constructed to describe vertical deformation characteristics.Typical distribution characteristics of longitudinal fractures and their length and distribution characteristics are also described.The grey correlation analysis theory was utilized to analyze the relationship between deformation characteristics of sections,cross sections and monitoring points and longitudinal crack characteristics(length and location).The analysis reveals a linear positive correlation or a high correlation between several indicators.This study can provide a deeper understanding of the occurrence and development mechanism of longitudinal cracks in asphalt pavement of cold areas,and give references for the research of road engineering structure,materials and distress prevention.

Are cyclones in Jupiter's polar regions modulated by the radially directional Rayleigh–Taylor instability?

The persistence and symmetry of cyclones around the poles of Jupiter are unknown.In the present investigation,inspired by cyclones at the South Pole of the Earth,we propose a mechanism that provides an explanation for this problem.The negative temperature gradient with respect to latitude may play an important role here.This temperature gradient is induced by solar radiation because of the small axial inclination of Jupiter.Our numerical simulations suggest that cyclones in the polar regions of Jupiter may be modulated or controlled by the radially directional Rayleigh–Taylor instability,driven by centrifugal force and the negative temperature gradient along the latitude.

Paired regions for shadow removal approach based on multi-features

The existence of shadow leads to the degradation of the image qualities and the defect of ground object information.Shadow removal is therefore an essential research topic in image processing filed.The biggest challenge of shadow removal is how to restore the content of shadow areas correctly while removing the shadow in the image.Paired regions for shadow removal approach based on multi-features is proposed, in which shadow removal is only performed on related sunlit areas.Feature distance between regions is calculated to find the optimal paired regions with considering of multi-features(texture, gradient feature, etc.) comprehensively.Images in different scenes with peak signal-to-noise ratio(PSNR) and structural similarity(SSIM) evaluation indexes are chosen for experiments.The results are shown with six existing comparison methods by visual and quantitative assessments, which verified that the proposed method shows excellent shadow removal effect, the brightness, color of the removed shadow area, and the surrounding non-shadow area can be naturally fused.

The effect of living environment on developmental disorders in cold regions

Developmental disorders(DDs)are a kind of chronic maladies,which can cause serious irreversible detriment to children’s physical and mental health.It is predominantly regulated by the interaction of environment and heredity.Cold regions are mainly located in the high latitudes of China.Their living environment is characterized by frequent cold wave,huge temperature difference,severe air pollution,high calorie diet,less exercise,smoking,drinking,etc.In recent years,substantial advances have been made in studies of the correlation between the living environment features in cold regions and the DDs.Accordingly,this article reviews the impact of the peculiar living environment of cold regions on DDs,with a view to provide fresh prevention strategies for reducing the morbidity of DDs in China cold regions by ameliorating living environment.

Research Progress of High-Quality and Equilibrium Economic Development of Ethnic Regions in the Context of Digital Economy

A new generation of information technology is driving the growth of digital economy across the world.In recent years,China has attached great importance to the development of digital economy and digital technology.With the support of a series of policies,the digital industry has been able to develop at an astounding rate,and at the same time the integration of other industries with digital technology has been strengthened.The profound changes in production factors,production methods,and production approaches have promoted high-quality regional economic development.Literature statistics have shown an increasing trend in digital economy research in recent years.In this paper,literature on digital economy development,digital economy and high-quality economic development,the current situation of economic development in ethnic regions,and digital economy and economic development in ethnic regions is recapitulated in an attempt to learn from existing studies.

Can climate change influence agricultural GTFP in arid and semi-arid regions of Northwest China?

There are eight provinces and autonomous regions(Gansu Province,Ningxia Hui Autonomous Region,Xinjiang Uygur Autonomous Region,Inner Mongolia Autonomous Region,Tibet Autonomous Region,Qinghai Province,Shanxi Province,and Shaanxi Province)in Northwest China,most areas of which are located in arid and semi-arid regions(northwest of the 400 mm precipitation line),accounting for 58.74%of the country's land area and sustaining approximately 7.84×10^6 people.Because of drought conditions and fragile ecology,these regions cannot develop agriculture at the expense of the environment.Given the challenges of global warming,the green total factor productivity(GTFP),taking CO2 emissions as an undesirable output,is an effective index for measuring the sustainability of agricultural development.Agricultural GTFP can be influenced by both internal production factors(labor force,machinery,land,agricultural plastic film,diesel,pesticide,and fertilizer)and external climate factors(temperature,precipitation,and sunshine duration).In this study,we used the Super-slacks-based measure(Super-SBM)model to measure agricultural GTFP during the period 2000-2016 at the regional level.Our results show that the average agricultural GTFP of most provinces and autonomous regions in arid and semi-arid regions underwent a fluctuating increase during the study period(2000-2016),and the fluctuation was caused by the production factors(input and output factors).To improve agricultural GTFP,Shaanxi,Shanxi,and Gansu should reduce agricultural labor force input;Shaanxi,Inner Mongolia,Gansu,and Shanxi should decrease machinery input;Shaanxi,Inner Mongolia,Xinjiang,and Shanxi should reduce fertilizer input;Shaanxi,Xinjiang,Gansu,and Ningxia should reduce diesel input;Xinjiang and Gansu should decrease plastic film input;and Gansu,Shanxi,and Inner Mongolia should cut pesticide input.Desirable output agricultural earnings should be increased in Qinghai and Tibet,and undesirable output(CO2 emissions)should be reduced in Inner Mongolia,Xinjiang,Gansu,and Shaanxi.Agricultural GTFP is influenced not only by internal production factors but also by external climate factors.To determine the influence of climate factors on GTFP in these provinces and autonomous regions,we used a Geographical Detector(Geodetector)model to analyze the influence of climate factors(temperature,precipitation,and sunshine duration)and identify the relationships between different climate factors and GTFP.We found that temperature played a significant role in the spatial heterogeneity of GTFP among provinces and autonomous regions in arid and semi-arid regions.For Xinjiang,Inner Mongolia,and Tibet,a suitable average annual temperature would be in the range of 7℃-9℃;for Gansu,Shanxi,and Ningxia,it would be 11℃-13℃;and for Shaanxi,it would be 15℃-17℃.Stable climatic conditions and more efficient production are prerequisites for the development of sustainable agriculture.Hence,in the agricultural production process,reducing the redundancy of input factors is the best way to reduce CO2 emissions and to maintain temperatures,thereby improving the agricultural GTFP.The significance of this study is that it explores the impact of both internal production factors and external climatic factors on the development of sustainable agriculture in arid and semi-arid regions,identifying an effective way forward for the arid and semi-arid regions of Northwest China.

Potential of rooftop rainwater harvesting to meet outdoor water demand in arid regions

The feasibility of rooftop rainwater harvesting （RRWH） as an alternative source of water to meet the outdoor water demand in nine states of the U.S. was evaluated using a system dynamics model developed in Systems Thinking, Experimental Learning Laboratory with Animation. The state of Arizona was selected to evaluate the effects of the selected model parameters on the efficacy of RRWH since among the nine states the arid region of Arizona showed the least potential of meeting the outdoor water demand with rain harvested water. The analyses were conducted on a monthly basis across a 10-year projected period from 2015 to 2024. The results showed that RRWH as a potential source of water was highly sensitive to certain model parameters such as the outdoor water demand, the use of desert landscaping, and the percentage of existing houses with RRWH. A significant difference （as high as 37.5%） in rainwater potential was observed between the projected wet and dry climate conditions in Arizona. The analysis of the dynamics of the storage tanks suggested that a 1.0-2.0 m3 rainwater barrel, on an average, can store approximately 80% of the monthly rainwater generated from the rooftops in Arizona, even across the high seasonal variation. This interactive model can be used as a quick estimator of the amount of water that could be generated, stored, and utilized through RRWH systems in the U.S. under different climate conditions. The findings of such comprehensive analyses may help regional policymakers, especially in arid regions, to develop a sustainable water management infrastructure.

Effects of uniconazole with or without micronutrient on the lignin biosynthesis,lodging resistance,and winter wheat production in semiarid regions

Lodging stress results in grain yield and quality reduction in wheat. Uniconazole, a potential plant growth regulator significantly enhances lignin biosynthesis and thus provides mechanical strength to plants in order to cope with lodging stress. A field study was conducted during the 2015–2016 and 2016–2017 growing seasons, to investigate the effects of uniconazole sole application or with micronutrient on the lignin biosynthesis, lodging resistance, and production of winter wheat. In the first experiment, uniconazole at concentrations of 0(CK), 15(US1), 30(US2), and 45(US3) mg L^-1 was applied as sole seed soaking, while in the second experiment with manganese(Mn) at concentration of 0.06 g L^-1 Mn, 0.06 g L^-1 Mn+ 15 mg L^-1 uniconazole(UMS1), 0.06 g L^-1 Mn+30 mg L^-1 uniconazole(UMS2), and 0.06 g L^-1 Mn+45 mg L^-1 uniconazole(UMS3), respectively. Uniconazole sole application or with micronutrient significantly increased the lignin content by improving the lignin-related enzyme activities of phenylalanine ammonia-lyase, cinnamyl alcohol dehydrogenase, tyrosine ammonialyase, and peroxidase, ameliorating basal internode characteristics, and breaking strength. The spike length, spike diameter, spikes/plant, weight/spike, yield/spike, and grain yield increased and then decreased with uniconazole application at a higher concentration, where their maximum values were recorded with UMS2 and US2 treatments. The lignin accumulation was positively correlated with lignin-related enzyme activities and breaking strength while, negatively correlated with lodging rate. Uniconazole significantly improved the lignin biosynthesis, lodging resistance, and grain yield of winter wheat and the treatments which showed the greatest effects were uniconazole seed soaking with micronutrient at a concentration of 30 mg L^-1 and 0.06 g L^-1 , and uniconazole sole seed soaking at a concentration of 30 mg L^-1 .

Detection of hyper-conserved regions in hepatitis B virus X gene potentially useful for gene therapy

AIM To detect hyper-conserved regions in the hepatitis B virus(HBV) X gene(HBX) 5' region that could be candidates for gene therapy.METHODS The study included 27 chronic hepatitis B treatmentnaive patients in various clinical stages(from chronic infection to cirrhosis and hepatocellular carcinoma, both HBeA g-negative and HBeA g-positive), and infected with HBV genotypes A-F and H. In a serum sample from each patient with viremia > 3.5 log IU/m L, the HBX 5' end region [nucleotide(nt) 1255-1611] was PCRamplified and submitted to next-generation sequencing(NGS). We assessed genotype variants by phylogenetic analysis, and evaluated conservation of this region by calculating the information content of each nucleotide position in a multiple alignment of all unique sequences(haplotypes) obtained by NGS. Conservation at the HBx protein amino acid(aa) level was also analyzed.RESULTS NGS yielded 1333069 sequences from the 27 samples, with a median of 4578 sequences/sample(2487-9279, IQR 2817). In 14/27 patients(51.8%), phylogenetic analysis of viral nucleotide haplotypes showed a complex mixture of genotypic variants. Analysis of the information content in the haplotype multiple alignments detected 2 hyper-conserved nucleotide regions, one in the HBX upstream non-coding region(nt 1255-1286) and the other in the 5' end coding region(nt 1519-1603). This last region coded for a conserved amino acid region(aa 63-76) that partially overlaps a Kunitz-like domain.CONCLUSION Two hyper-conserved regions detected in the HBX 5' end may be of value for targeted gene therapy, regardless of the patients' clinical stage or HBV genotype.