Regulator of G protein signaling 6 mediates exercise-induced recovery of hippocampal neurogenesis,learning,and memory in a mouse model of Alzheimer’s disease

Hippocampal neuronal loss causes cognitive dysfunction in Alzheimer’s disease.Adult hippocampal neurogenesis is reduced in patients with Alzheimer’s disease.Exercise stimulates adult hippocampal neurogenesis in rodents and improves memory and slows cognitive decline in patients with Alzheimer’s disease.However,the molecular pathways for exercise-induced adult hippocampal neurogenesis and improved cognition in Alzheimer’s disease are poorly understood.Recently,regulator of G protein signaling 6(RGS6)was identified as the mediator of voluntary running-induced adult hippocampal neurogenesis in mice.Here,we generated novel RGS6fl/fl;APP_(SWE) mice and used retroviral approaches to examine the impact of RGS6 deletion from dentate gyrus neuronal progenitor cells on voluntary running-induced adult hippocampal neurogenesis and cognition in an amyloid-based Alzheimer’s disease mouse model.We found that voluntary running in APP_(SWE) mice restored their hippocampal cognitive impairments to that of control mice.This cognitive rescue was abolished by RGS6 deletion in dentate gyrus neuronal progenitor cells,which also abolished running-mediated increases in adult hippocampal neurogenesis.Adult hippocampal neurogenesis was reduced in sedentary APP_(SWE) mice versus control mice,with basal adult hippocampal neurogenesis reduced by RGS6 deletion in dentate gyrus neural precursor cells.RGS6 was expressed in neurons within the dentate gyrus of patients with Alzheimer’s disease with significant loss of these RGS6-expressing neurons.Thus,RGS6 mediated voluntary running-induced rescue of impaired cognition and adult hippocampal neurogenesis in APP_(SWE) mice,identifying RGS6 in dentate gyrus neural precursor cells as a possible therapeutic target in Alzheimer’s disease.

Pyroptosis,ferroptosis,and autophagy in spinal cord injury:regulatory mechanisms and therapeutic targets

Regulated cell death is a form of cell death that is actively controlled by biomolecules.Several studies have shown that regulated cell death plays a key role after spinal cord injury.Pyroptosis and ferroptosis are newly discovered types of regulated cell deaths that have been shown to exacerbate inflammation and lead to cell death in damaged spinal cords.Autophagy,a complex form of cell death that is interconnected with various regulated cell death mechanisms,has garnered significant attention in the study of spinal cord injury.This injury triggers not only cell death but also cellular survival responses.Multiple signaling pathways play pivotal roles in influencing the processes of both deterioration and repair in spinal cord injury by regulating pyroptosis,ferroptosis,and autophagy.Therefore,this review aims to comprehensively examine the mechanisms underlying regulated cell deaths,the signaling pathways that modulate these mechanisms,and the potential therapeutic targets for spinal cord injury.Our analysis suggests that targeting the common regulatory signaling pathways of different regulated cell deaths could be a promising strategy to promote cell survival and enhance the repair of spinal cord injury.Moreover,a holistic approach that incorporates multiple regulated cell deaths and their regulatory pathways presents a promising multi-target therapeutic strategy for the management of spinal cord injury.

Functions of nuclear factor Y in nervous system development,function and health

Nuclear factor Y is a ubiquitous heterotrimeric transcription factor complex conserved across eukaryotes that binds to CCAAT boxes,one of the most common motifs found in gene promoters and enhancers.Over the last 30 years,research has revealed that the nuclear factor Y complex controls many aspects of brain development,including differentiation,axon guidance,homeostasis,disease,and most recently regeneration.However,a complete understanding of transcriptional regulatory networks,including how the nuclear factor Y complex binds to specific CCAAT boxes to perform its function remains elusive.In this review,we explore the nuclear factor Y complex’s role and mode of action during brain development,as well as how genomic technologies may expand understanding of this key regulator of gene expression.

A Capacitor-Free CMOS Low-Dropout Regulator for System-on-Chip Application

A stable CMOS low drop-out regulator without an off-chip capacitor for system-on-chip application is presen- ted. By using an on-chip pole splitting technique and an on-chip pole-zero canceling technique, high stability is achieved without an off-chip capacitor. The chip was implemented in CSMC＇s 0.5μm CMOS technology and the die area is 600μm×480μm. The error of the output voltage due to line variation is less than -＋ 0.21% ,and the quiescent current is 39.8μA. The power supply rejection ratio at 100kHz is -33.9dB, and the output noise spectral densities at 100Hz and 100kHz are 1.65 and 0.89μV √Hz, respectively.

Model Predictive Control Synthesis Approach of Electrode Regulator System for Electric Arc Furnace

In electric are furnace smelting, electrode regulator system is a key link. A good electrode control algorithm will reduce energy consumption effectively and shorten smelting time greatly. The offline design online synthesis model predictive control algorithm is proposed for electrode regulator system with input and output constraints. On the offline computation, the continuum of terminal constraint sets will be constructed. On the online synthesis, the time-varying terminal constraint sets will be adopted and at least one free control variable will be introduced to solve the min-max optimization control problem. Then Lyapunov method will be adopted to analyze closed-loop system stability. Simulation and field trial results show that the proposed offline design online synthesis model predictive control method is effective.

Establishment of high frequency shoot regeneration system in Himalayan poplar(Populus ciliata Wall. ex Royle) from petiole explants using Thidiazuron cytokinin as plant growth regulator

Populus species are important resources for industry and in scientific study on biological and agricul- tural systems. Our objective was to enhance the frequency of plant regeneration in Himalayan poplar （Populus ciliata wall. ex Royle）. The effect of TDZ alone and in combi- nation with adenine and NAA was studied on the regen- eration potential of petiole explants. The explants were excised from Himalayan poplar plants grown in glass- houses. After surface sterilization the explants were cul- tured on shoot induction medium. High percentage shoot regeneration （86 %） was recorded on MS medium sup- plemented with 0.004 mg L-1 TDZ and 79.7 mg L-1 adenine. The regenerated shoots for elongation and multi- plication were transferred to MS ＋ 0.5 mg L-1 BAP ＋ 0.2 mg L-1 IAA ＋ 0.3 mg L-1 GA3. Root re- generation from shoots developed in vitro was observed on MS medium supplemented with 0.10 mg L-1 IBA. Hi- malayan poplar plantlets could be produced within 2 months after acclimatization in a sterile mixture of sand and soil. We developed a high efficiency plant regeneration protocol from petiole explants of P. ciliata.

Is erythroferrone finally the long sought-after systemic erythroid regulator of iron?

Iron metabolism is regulated on the cellular and the systemic level. Over the last decade, the liver peptide "hepcidin" has emerged as the body's key irons store regulator. The long postulated "erythroid regulator of iron", however, remained elusive. Last year, evidence was provided, that a previously described myokine "myonectin" may also function as the long sought erythroid regulator of iron. Myonectin was therefore renamed "erythroferrone". This editorial provides a brief discussion on the two functions of erythroferrone and also briefly considers the emerging potential role of transferrin receptor 2 in erythropoiesis.

Adaptive Linear Quadratic Regulator for Continuous-Time Systems With Uncertain Dynamics

In this paper, adaptive linear quadratic regulator(LQR) is proposed for continuous-time systems with uncertain dynamics. The dynamic state-feedback controller uses inputoutput data along the system trajectory to continuously adapt and converge to the optimal controller. The result differs from previous results in that the adaptive optimal controller is designed without the knowledge of the system dynamics and an initial stabilizing policy. Further, the controller is updated continuously using input-output data, as opposed to the commonly used switched/intermittent updates which can potentially lead to stability issues. An online state derivative estimator facilitates the design of a model-free controller. Gradient-based update laws are developed for online estimation of the optimal gain. Uniform exponential stability of the closed-loop system is established using the Lyapunov-based analysis, and a simulation example is provided to validate the theoretical contribution.

Application of Single Neuron Adaptive PID Regulators with Auto-tuning Gain in Industrial Parameter(Pressure)Closed-loop Process Control Systems

A type of single neuron adaptive PID regulator with auto-tuning gain is proposed and applied to the work control of fans, waterpumps and air-pressers etc. in Handan Iron & Steel Compel China. The robusthess of induStrial parameter closed-loop process controlsystems is improved, and the work quality of the systems bettered.

REGULATION OF INTESTINAL DYSBACTERIOSIS OF BURNED RATS BY MICROECOSYSTEM REGULATORS

Objective To investigate the feasibility of using synbiotics and probiotics to prevent and cure in- testinal dysbacteriosis after burn. Methods Burned rats were fed with synbiotics and probiotics reagents. and the amounts of major intestinal florae in caecal contents were detected. Results The major physiological anaerobes were mostly stable, and the conditioned pathogens had no abnormal. Conclusion Tke micro-ecosystem regulator can quickly supplement the decreased physiological anaerobes caused by burning,and avoid the occurrence of dysbacterio- sis.

Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis

BACKGROUND Acute liver failure(ALF)has a high mortality with widespread hepatocyte death involving ferroptosis and pyroptosis.The silent information regulator sirtuin 1(SIRT1)-mediated deacetylation affects multiple biological processes,including cellular senescence,apoptosis,sugar and lipid metabolism,oxidative stress,and inflammation.AIM To investigate the association between ferroptosis and pyroptosis and the upstream regulatory mechanisms.METHODS This study included 30 patients with ALF and 30 healthy individuals who underwent serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)testing.C57BL/6 mice were also intraperitoneally pretreated with SIRT1,p53,or glutathione peroxidase 4(GPX4)inducers and inhibitors and injected with lipopolysaccharide(LPS)/D-galactosamine(D-GalN)to induce ALF.Gasdermin D(GSDMD)^(-/-)mice were used as an experimental group.Histological changes in liver tissue were monitored by hematoxylin and eosin staining.ALT,AST,glutathione,reactive oxygen species,and iron levels were measured using commercial kits.Ferroptosis-and pyroptosis-related protein and mRNA expression was detected by western blot and quantitative real-time polymerase chain reaction.SIRT1,p53,and GSDMD were assessed by immunofluorescence analysis.RESULTS Serum AST and ALT levels were elevated in patients with ALF.SIRT1,solute carrier family 7a member 11(SLC7A11),and GPX4 protein expression was decreased and acetylated p5,p53,GSDMD,and acyl-CoA synthetase long-chain family member 4(ACSL4)protein levels were elevated in human ALF liver tissue.In the p53 and ferroptosis inhibitor-treated and GSDMD^(-/-)groups,serum interleukin(IL)-1β,tumour necrosis factor alpha,IL-6,IL-2 and C-C motif ligand 2 levels were decreased and hepatic impairment was mitigated.In mice with GSDMD knockout,p53 was reduced,GPX4 was increased,and ferroptotic events(depletion of SLC7A11,elevation of ACSL4,and iron accumulation)were detected.In vitro,knockdown of p53 and overexpression of GPX4 reduced AST and ALT levels,the cytostatic rate,and GSDMD expression,restoring SLC7A11 depletion.Moreover,SIRT1 agonist and overexpression of SIRT1 alleviated acute liver injury and decreased iron deposition compared with results in the model group,accompanied by reduced p53,GSDMD,and ACSL4,and increased SLC7A11 and GPX4.Inactivation of SIRT1 exacerbated ferroptotic and pyroptotic cell death and aggravated liver injury in LPS/D-GalNinduced in vitro and in vivo models.CONCLUSION SIRT1 activation attenuates LPS/D-GalN-induced ferroptosis and pyroptosis by inhibiting the p53/GPX4/GSDMD signaling pathway in ALF.

Endogenous bioelectric fields: a putative regulator of wound repair and regeneration in the central nervous system

Studies on a variety of highly regenerative tissues, including the central nervous system（CNS） in non-mammalian vertebrates, have consistently demonstrated that tissue damage induces the formation of an ionic current at the site of injury. These injury currents generate electric fields（EF） that are 100-fold increased in intensity over that measured for uninjured tissue. In vitro and in vivo experiments have convincingly demonstrated that these electric fields（by their orientation, intensity and duration） can drive the migration, proliferation and differentiation of a host of cell types. These cellular behaviors are all necessary to facilitate regeneration as blocking these EFs at the site of injury inhibits tissue repair while enhancing their intensity promotes repair. Consequently, injury-induced currents, and the EFs they produce, represent a potent and crucial signal to drive tissue regeneration and repair. In this review, we will discuss how injury currents are generated, how cells detect these currents and what cellular responses they can induce. Additionally, we will describe the growing evidence suggesting that EFs play a key role in regulating the cellular response to injury and may be a therapeutic target for inducing regeneration in the mammalian CNS.

Adjusting the Parameters of PI Regulator in All-electrical Tank Gun Control System by Computer Program

Both the traverse subsystem and the elevation subsystem of the all-electrical tank gun control system are composed of electrical drive control system respectively. The parameters of PI regulator in these electrical drive control systems affect the performance of the control system seriously. Up to now, there is not a simple and practical method for choosing regulator parameters, which are usually determined by repeated and continual readjustment. This method is low efficient, and the parameters got are not always optimal. A method for on-line adjusting the parameters of PI regulator in the electrical drive control system by computer program is introduced in this paper. The function of adjusting PI parameters of the electrical drive control system is realized by PC program written by VC++ and controlling program written by assemble language and by the communication between PC and DSP completed by the control MSCOMM in VC++6.0. The method as mentioned above which is applied for an all-electrical tank gun control system under development is proved very available, a better performance might be obtained for the all-electrical tank gun control system easily.

An NMOS output-capacitorless low-dropout regulator with dynamic-strength event-driven charge pump

In this paper,an NMOS output-capacitorless low-dropout regulator(OCL-LDO)featuring dual-loop regulation has been proposed,achieving fast transient response with low power consumption.An event-driven charge pump(CP)loop with the dynamic strength control(DSC),is proposed in this paper,which overcomes trade-offs inherent in conventional structures.The presented design addresses and resolves the large signal stability issue,which has been previously overlooked in the event-driven charge pump structure.This breakthrough allows for the full exploitation of the charge-pump structure's poten-tial,particularly in enhancing transient recovery.Moreover,a dynamic error amplifier is utilized to attain precise regulation of the steady-state output voltage,leading to favorable static characteristics.A prototype chip has been fabricated in 65 nm CMOS technology.The measurement results show that the proposed OCL-LDO achieves a 410 nA low quiescent current(IQ)and can recover within 30 ns under 200 mA/10 ns loading change.

A voltage regulator system with dynamic bandwidth boosting for passive UHF RFID transponders

This paper presents a voltage regulator system for passive UHF RFID transponders, which contains a rectifier, a limiter, and a regulator. The rectifier achieves power by rectifying the incoming RF energy. Due to the huge variation of the rectified voltage, a limiter at the rectifier output is used to clamp the rectified voltage. In this paper, the design of a limiter circuit is discussed in detail, which can provide a stable limiting voltage with low sensitivity to temperature variation and process dispersion. The key aspect of the voltage regulator system is the dynamic bandwidth boosting in the regulator. By sensing the excess current that is bypassed in the limiter during periods of excess energy, the bias current as well as the bandwidth of the regulator are increased, the output supply voltage can recover quickly from line transients during the periods of no RF energy to a full blast of RF energy. This voltage regulator system is implemented in a 0.18μm CMOS process.

Research on the Pressure Regulator Effect on a Pneumatic Vibration Isolation System

The pneumatic vibration isolator(PVI)plays an increasingly important role in precision manufacturing.In this paper,aiming to detect the performance of the pressure regulator in the PVI system,a PVI testing system with a pressure regulator is designed and developed.Firstly,the structure of the pneumatic spring is presented and analyzed,and the nonlinear stiffness is obtained based on the ideal gas model and material mechanics.Then,according to the working principle and continuity equations of ideal airflow,a dynamic model of the PVI system with a pressure regulator is established.Through the simulation analysis,the vibration isolation performance is improved with the efficient and precise pressure regulator.The average values of both the vibration velocity and transmission rate decrease when the vibration is set to 4,10,20 and 40 Hz,respectively.The experiments demonstrate the reliability and effectiveness of the pressure regulator.This achievement will become an important basis for future research concerning precision manufacturing.

Localization of Voltage Regulators in Distribution Systems by a Mixed Genetic–Tabu Search Algorithm

The optimal allocation of regulators banks in distribution systems is a merely combinatorial problem in which the best points of installation correspond to the best benefit, considering the admitted objective function, without violating and operating limits. The objective function must be chosen so that its value represents the operation state of the system. As the problem possesses combinatorial nature, its complexity will increase exponentially with the number of possibilities. Systems with large numbers of nodes and / or with the possibility of installing more than one bank require a large number of calculations to find the solution. An additional issue is the fact that the problem does not have a continuous nature, presenting discontinuity points in the objective function, limiting the application of optimization methods based on gradients. Based on the nature of the problem two optimization methods were used to solve the problem: Genetic Algorithm (GA) and modified Tabu Search (TS). The GA function will scour the search space and find regions with local minima that are candidates to be the solution. On the other hand the TS provides local search in the regions defined by GA so that the overall optimum is achieved.

Investigation into PCB Routing Loss for Coupled Inductor Based VR (Voltage Regulator) Design in Server Computing System

Coupled inductor is one appealing technology to improve transient response and reduce output decoupling significantly in interleaved multi-phase voltage regulators (VRs). One well known problem is that coupled inductor winding structure causes PCB routing path longer than discrete inductor design. This paper investigates possible PCB routing schemes for coupled inductor and conducts a fair and quantitative comparison with discrete inductor in a server VR design. Simulation and measurement are also conducted to verify the analysis.

Adaptation in Stochastic Dynamic Systems—Survey and New Results III: Robust LQ Regulator Modification

The paper is intended to provide algorithmic and computational support for solving the frequently encountered linear-quadratic regulator (LQR) problems based on receding-horizon control methodology which is most applicable for adaptive and predictive control where Riccati iterations rather than solution of Algebraic Riccati Equations are needed. By extending the most efficient computational methods of LQG estimation to the LQR problems, some new algorithms are formulated and rigorously substantiated to prevent Riccati iterations divergence when cycled in computer implementation. Specifically developed for robust LQR implementation are the two-stage Riccati scalarized iteration algorithms belonging to one of three classes: 1) Potter style (square-root), 2) Bierman style (LDLT), and 3) Kailath style (array) algorithms. They are based on scalarization, factorization and orthogonalization techniques, which allow more reliable LQR computations. Algorithmic templates offer customization flexibility, together with the utmost brevity, to both users and application programmers, and to ensure the independence of a specific computer language.

Takagi-Sugeno Fuzzy Regulator Design for Nonlinear and Unstable Systems Using Negative Absolute Eigenvalue Approach

This paper introduces a Takagi-Sugeno(T-S)fuzzy regulator design using the negative absolute eigenvalue(NAE)approach for a class of nonlinear and unstable systems.The open-loop system is initially embodied by the traditional T-S fuzzy model and then,all closed-loop subsystems are combined using the proposed Max-Min operator in place of traditional weighted average operator from the controller side to lessen the coupling virtually and simplify the proposed regulator design.For each virtually decoupled closed-loop subsystem,the composite regulators(i.e.,primary and secondary regulators)are designed by the NAE approach based on the enhanced eigenvalue analysis.The Lyapunov function is utilized to guarantee the asymptotic stability of the overall T-S fuzzy control system.The most popular and widely used nonlinear and unstable systems like the electromagnetic levitation system(EMLS)and the inverted cart pendulum(ICP)are simulated for the wide range of the initial conditions and the enormous variation in the disturbance.The transient and steady-state performance of the considered systems using the proposed design are analyzed in terms of the decay rate,settling time and integral errors as IAE,ISE,ITAE,and ITSE to validate the effectiveness of the proposed approach compared to the most popular and traditional parallel distributed compensation(PDC)approach.