Time series analysis of correlativity between pulmonary tuberculosis and seasonal meteorological factors based on theory of Human-Environmental Inter Relation

Objective:This paper aims to study the correlativity between the number of pulmonary tuberculosis (PTB) cases and seasonal meteorological factors in Beijing.Methods:Based on theory of Human-Environmental Inter Relation in Huangdi's Internal Classics,we adopted monthly cases of PTB in Beijing from 2004 to 2011,and established a Seasonal Autoregressive Integrated Moving Average (SARIMA) model.Using the cross-correlation function (CCF),we then analyzed the correlation between meteorological factors and number of infected patients.The related meteorological factors were subsequently integrated,to establish a Seasonal Autoregressive Integrated Moving Average with explanatory variables (SARIMAX) model,which was used to estimate and verify the number of PTB cases in 2012.Results:In this study,a SARIMA(0,1,1) (0,1,1)12 model was established;CCF analysis was used to reveal the correlativity between PTB and precipitation with 1 lag,relative humidity with 1 lag.Then,integrated with relative humidity with 1 lag (β =2.405,95% confidence interval:0.433-4.377),the SARIMAX prediction model was proved to be an accurate approach for predicting local situations of PTB occurrence.Conclusions:The occurrence of PTB is correlated with seasonal meteorological factors.Combining these factors,an exact prediction model can be established,to estimate of the number of PTB infected patients.

Research on the Relationship between Job Satisfaction and Harmony in Human Relations among New Female Generation

In the area of knowledge economy, the new generation of cmployccs is becoming the main force in the talent market. And the new female generation has more distinctive features as a special group. How to effectively manage the new generation employees and have them work weU has been the urgent problem that the manager should be solved. Based on previous studies, the thesis discusses the related factors that would affect the new female generation employee＇ s wok satisfaction with related research method. So that to make contributions to the permanent performance of enterprise.

Correlation between human seizure-related gene 6 variants and idiopathic generalized epilepsy in a Southern Chinese Han population

This study sought to analyze the genotype and gene mutations of human seizure-related gene 6 in 98 patients with idiopathic generalized epilepsy （non-febrile seizures）, who were selected from three generations of the Chinese Han population living in Shanghai, Zhejiang Province, Wuxi of Jiangsu Province, and Jiangxi Province of Southern China. Twenty-six patients＇ parents were available as a first-degree relatives group and 100 biologically unrelated healthy controls were collected as the control group. Based on the age of onset and seizure type, the patients were divided into six subgroups. Polymerase chain reaction and DNA direct sequencing analysis showed that the most frequent mutations c. 1249dupC （p.Gly418Argfx31 ） and c.1636A 〉 G （p.Thr546Ala） were detected in some idiopathic generalized epilepsy patients and tl^eir asymptomatic first-degree relatives （30.6% vs. 19.2% and 11.2% vs. 26.9%）. A novel mutation c.1807G 〉A （p.Val603Met） was found in a patient with late-onset idiopathic generalized epilepsy. There was no significant difference in the incidence of these three mutations among the different subgroups of idiopathic generalized epilepsy and controls. Thus, further analysis of a larger population is needed to confirm the assumption that human seizure-related gene 6 is a susceptibility gene for idiopathic generalized epilepsy with various sub-syndromes.

Quantitative distinction of the relative actions of climate change and human activities on vegetation evolution in the Yellow River Basin of China during 1981-2019

Under the combined influence of climate change and human activities,vegetation ecosystem has undergone profound changes.It can be seen that there are obvious differences in the evolution patterns and driving mechanisms of vegetation ecosystem in different historical periods.Therefore,it is urgent to identify and reveal the dominant factors and their contribution rates in the vegetation change cycle.Based on the data of climate elements(sunshine hours,precipitation and temperature),human activities(population intensity and GDP intensity)and other natural factors(altitude,slope and aspect),this study explored the spatial and temporal evolution patterns of vegetation NDVI in the Yellow River Basin of China from 1989 to 2019 through a residual method,a trend analysis,and a gravity center model,and quantitatively distinguished the relative actions of climate change and human activities on vegetation evolution based on Geodetector model.The results showed that the spatial distribution of vegetation NDVI in the Yellow River Basin showed a decreasing trend from southeast to northwest.During 1981-2019,the temporal variation of vegetation NDVI showed an overall increasing trend.The gravity centers of average vegetation NDVI during the study period was distributed in Zhenyuan County,Gansu Province,and the center moved northeastwards from 1981 to 2019.During 1981-2000 and 2001-2019,the proportion of vegetation restoration areas promoted by the combined action of climate change and human activities was the largest.During the study period(1981-2019),the dominant factors influencing vegetation NDVI shifted from natural factors to human activities.These results could provide decision support for the protection and restoration of vegetation ecosystem in the Yellow River Basin.

The Profound Revelation of Human Social Relations and the Profound Realization of Information Right of Citizens——The social significance of network technique advancement in the perspective of “Entity Cube”

The R＆D group of relationship searching in Microsoft Asia Research Institute explains ＂Entity Cube＂ launched in 2008 as follows：

Low-carbon Landscape Construction Approaches Based on Human Behavior

After the analysis of connotations of human behavior and low-carbon landscape,according to relevant theories of behavioral science and psychology,the internal relation between behavior and lowcarbon landscape has been discussed.They relate to,interact on and are the precondition of each other.Reasonable landscape environment has carried people's expectation for nature,and different landscapes give people different feelings and present to be of different behavior features.Finally,the paper has proposed constructing low-carbon landscape in the perspective of landscape design idea,design methods,construction process and later management,providing a theoretical basis for advocating energy saving and environmental protection,and creating green ecology.

ISOLATION AND IDENTIFICATION OF THE RELATED GENE MEDIATING γ-RADIATION-INDUCED APOPTOSIS IN HUMAN HL- 60 CELLS

To identify the member of the caspase family proteases involved in γ radiation induced apoptosis in HL 60 cells, using degenerated oligonucleotide primers encoding the highly conserved peptides, which were present in all known caspases, RT PCR was performed on poly (A) RNA from the γ radiation induced apoptotic HL 60 cells. Then, cloned and sequenced to identify the amplified DNA fragments. The results showed that the amplified DNA fragments were identified with a part of caspase 3 cDNA. It indicated that caspase 3 was involved in γ radiation induced apoptosis in HL 60 cells and may be the pivotal element of radiation induced apoptosis.

Studies on Human Dietary Requirements and Safe Range of Dietary Intakes of Selenium in China and Their Application in the Prevention of Related Endemic Diseases

The human dietary selenium requirement in China has been estimated by various methods. The minimum dietary selenium requircment for the prevention of Keshan disease (KD) was found to be around 17 μg/d. On the other hand, an intake of 40 μg/d is required to maintain the plasma glutathione peroxidase (GPx) activity at plateau. Hence 40 μg/d is considered as the adequate dietary selenium requirement. Studies conducted in a chronic selenosis area indicate thai the toxic dietary selenlum inlake (adverse effect level), which would maintain the characteristic fingernail changes, was approximately 1600 μg/d. The mean value of dietary selenium intakes, which enabled the five patients to recover from fingernail lesions, was found to be 819 ±126μg/d. At a 95% confidence limit, the lower limit is around 600μg/d. Therefore, 600 and 400μg/d were suggested as the individual daily maximum safe selenium intake and the safe dietary selenium intake, respectively,The results were used in the prevention of Se-related endemic KD and Kashin-Beck disease (KBD)

The Changing Labour Relations during the Period of Economic Reform:A Case Study of Chinese Township and Village Enterprises in Yangtze River Delta

The economic reform launched in the People's Republic of China(China) led to a rapid growth in non-state sectors.Various forms of corporate governances and ownerships emerged during the last two decades,especially the Township and Village Enterprises owned by local residents.This article is conducted on the changing labour relations in Chinese Township and Village Enterprises(TVEs) despite their increasing contributions to China's transition from planned economy to market economy,and studies the labour market and the Labour Law to understand the unique labour mobility and to test the relationship between Human Resource Management(HRM) practice and the performance among TVEs.

Association between HLA-DR Expression and Multidrug-resistant Infection in Patients with Severe Acute Pancreatitis

Multidrug-resistant （MDR） bacterial infection is a common complication of severe acute pancreatitis （SAP）. This study aimed to explore the association between human leukocyte antigen-antigen D-related （HLA-DR） expression and multidrug-resistant infection in patients with SAP. A total of 24 SAP patients who were admitted to Nanjing Drum Tower Hospital between May 2015 and December 2016 were enrolled in the study. The percentages of CD4^＋, CD8^＋, natural killer （NK）, and HLA-DR （CD14＋） cells and the CD4^＋/CD8^＋ cell ratio on days 1, 7, 14, and 28 after admission were determined by flow cytometry. Eighteen patients presented with the symptoms of infection. Among them, 55.6% patients （10/18） developed MDR infection. The most common causative MDR organisms were Enterobacter cloacae and Acinetobacter baumannii. The CD4＋/CD8＋ cell ratio and the percentage of NK cells were similar between patients with non-MDR and patients with MDR infections. In patients without infection, the HLA-DR percentage was maintained at a high level throughout the 28 days. Compared to the patients without any infection, the HLA-DR percentage in patients with non-MDR infection was reduced on day 1 but increased and reached similar levels on day 28. In patients with MDR infection, the HLA-DR percentage remained below normal levels at all-time points. It was concluded that persistent down-regulation of HLA-DR expression is associated with MDR bacterial infection in patients with SAP.

Prokaryotic Expression and Preparation of Polyantibody of Human Histydyl-tRNA Synthetase Related Gene

Summary: The aim of this study was to express and purify human histydyl-tRNA synthetase related gene and to prepare its polyantibody. The open reading frame was amplified by PCR, and then recombined into prokaryotic expression vector pQE30 and transformed into E. coli M15 for expression. The expressed products were induced by IPTG after the reconstructed pQE30 was transferred into M15. After purified by Ni affinity chromatography, the product was identified to be a single band by SDS-PAGE. The rabbits were inoculated with purified products. High-titer polyantibody was successfully prepared. Highly-purified expression product and prepared polyantibody may provide a good basis for further study.

Development and evaluation of an ELISA method for the measurement of kallikrein-related peptidase 5 (KLK5) in human serum

Kallikrein-related peptidases (KLKs) have been proposed as potential cancer biomarkers. Kallikrein-related peptidase 5 (KLK5) is a secreted trypsin-like protease of the KLKs. Until now, detection of KLK5 in both biological fluids and tissues has been described frequently due to the potential of being a new cancer biomarker. Our objective was to prepare KLK5 antibodies and establish an ELISA method for KLK5 to study the possible clinical application of KLK5 as a biomarker for malignancies. In this study, recombinant KLK5 protein was produced and purified using a prokaryotic expression system, and then used as immunogen to generate antibodies. High titers of specific antibodies were measured in serum of rabbits after the forth booster injection. And the titer of the antiserum reached 1:106. We have also generated monoclonal antibodies using hybridoma technology and the titer reached 1:105. The activity of KLK5 antibodies was characterized by Western blot and immunohistochemistry. To quantitatively examine KLK5 in serum samples, we established double antibody sandwich ELISA method using mouse mAb as capture and rabbit pAb as tracer antibody. We have detected KLK5 levels in ovarian cancer serum to ensure that our sandwich ELISA measurement to have high sensitivity and specificity. The ranges of linearity reached by the standard curves of the newly developed ELISA were 0.45 ng/mL to 125 ng/mL. The detection limit of the method, defined as the concentration of KLK5 can be distinguished, was 0.20 ng/mL. Median serum KLK5 levels were 3.77 ng/mL and 0.86 ng/mL in ovarian cancer patients and normal female, respectively (P ELISA assay for KLK5. Our preliminary findings prompt that KLK5 may be a new potential biomarker for the diagnosis and prognosis in patients with ovarian.

Human Systems Therapy:Use of Interpersonal Relations in Promoting Therapeutic Change

The aim of this work is to present the central role of interpersonal relations in Human Systems Therapy(HST).Psychotherapies can be classified as(a)down-top therapies,such as individual therapies following reductionism,namely influencing the whole(behavior)through the intra-psychic parts,(b)top-down or holistic therapies,such as family therapies influencing the parts(individuals)through the whole family,and(c)the combination of down-top and top-down.Emotionally Focused Therapy(EFT)and Multisystemic Therapy(MST)act on two levels that influence each other,namely the individuals and their relations.This is also applied,more or less,on relational and interpersonal therapies.When there is an intervention in two interacting parts or levels,then the result of the intervention is more than additive and more effective.When the levels are more than two,then the effectiveness is expected to be even higher.This is the case of Human Systems Therapy(HST),which is applied intrapsychically,on interpersonal relations and on family and/or larger systems,with interpersonal relations having a central role.HST is based on general systems principles and it constitutes a multilevel approach:(a)a definition of a human system,(b)epistemology,(c)a model of human systems personality/intelligence,(d)an increase in order and variety.Besides,HST includes a large number of techniques.Empirical support of HST effectiveness shows that(a)after seven sessions with schizophrenics,39%of psychotic symptoms are reduced,(b)after eight sessions with hashish adolescent users,all users stop the use completely,and(c)9 out of 11 psychiatric non-psychotic cases(81%),after four sessions,become non-psychiatric ones(treated).

Human Capital Advanced Investment and Its Effects on Economic Growth

This paper created the Human Capital Relatively Advanced Investment(HCRAI)index to compare human capital investment in China and other countries.The HCRAI comprises life expectancy and average length of schooling adjusted for GDP per capita to measure the degree to which a country has invested in human capital in the early stage.Our study found that in 2014,China ranked much higher on the HCRAI index than on GDP per capita.The fact that China was far ahead of the United States on the HCRAI ranking suggests that China had invested more in human capital.Since the 1970s,China’s HCRAI ranking has experienced an inverted U-shaped curve,increasing at first and ranking first in the world in 1980 before declining in a later stage.That is to say,China has invested somewhat less on human capital in relative terms in the post-reform era.International data comparison showed that the HCRAI index may largely explain a country’s long-term economic growth rate,justifying the continuity in China’s six-decade development before and after reform and opening up in 1978.By replacing the existing absolute indicators with relative indicators,this paper measures the level of human care in China,reflecting the concept of fairness.The international comparison and long-term evolution of the HCRAI index offer a new perspective for the new normal of China’s economy and supply-side structural reforms.China’s declining HCRAI ranking over the past three decades indicates the importance of a more inclusive and sustainable development path that puts human first.

IMMUNOCHEMICAL IDENTIFICATION AND LOCALIZATION OF CYTOCHROME P-450HSjISOZYME, AN ENZYME RELATED TO NITROSAMINE METABOLISM, IN HUMAN GASTRIC MUCOSA AND GASTRIC CARCINOMA

Monoclonal antibody (MAb) to rat liver cyto-chrome P-450j isozyme, an activating enzyme specific to nitrosamine metabolism, was used coupled with immunoblotting, densitometer scanning of SDS-PAGE gels and immunohistochemical technique. The trace P-450HSj isozyme (Mr. 51.5 Kd) was found in human gastric mucosa. It was similar to P-450j in molecular weight, catalytic and immunochemical properties. The concentrations of P-450HSj in mucosa of lesser curvature were higher than those in greater curvature. This might be one of the important reasons that lesser curvature is the commonest area for gastric carcinoma. But there was possibly less P-450HSj in gastric mucosa with cancer. Im-munohistochemically, P-450HSj was discovered in the cytoplasm of some glandular epithelial cells, especially in the glands with hyperplastic and intestinal metaplastic changes adjacent to carcinoma. It was also found in some normal glands and in tumor cells of high-differentiated adenocarcinoma, but not in those of low-differentiated ones. Following subjects are discussed: (1) the method of detecting trace P-450HSj, (2) the rule of distribution of P-450HSj, and (3) the relationship between the isozyme and the occurrence of gastric cancer caused by nitrosa-mines.

Molecular cloning and characterization of human age-related NADH oxidase (arNOX) proteins as members of the TM9 superfamily of transmembrane proteins

Age-related NADH oxidase (arNOX = ENOX3) proteins are superoxide-generating cell surface oxidases that increase in activity with age beginning at about 30 y. A soluble and truncated exfoliated form of the activity is present in blood and other body fluids. The activity was purified to apparent homogeneity from human urine and resolved by 2-D gel electrophoresis into a series of 24 to 32 kDa components of low isoelectric point. The purified proteins were resistant both to N-terminal sequencing and trypsin cleavage. Cleavage with pepsin revealed peptides corresponding to the TM9 family of transmembrane proteins. Peptide antisera raised to all five members of the human TM9 family sequentially blocked the arNOX activity of human saliva and sera. The soluble truncated N-terminus of the human homolog TM9SF4 was expressed in bacteria. The recombinant protein was characterized biochemically and exhibited ar-NOX activity. The findings identify five arNOX isoforms each of which correspond to one of the five known TM9 family members. The exfoliated soluble arNOX forms are derived from the 24 to 32 kDa N-termini exposed to the cell’s exterior at the cell surface. Each of the shed forms contain putative functional motifs characteristic of ECTO-NOX (ENOX) proteins despite only minimal sequence identity. Our findings identify arNOX as having functional characteristics of ENOX proteins and the TM9 superfamily of proteins as the genetic origins of the five known arNOX isoforms present in human sera, plasma and other body fluids1.

The Conflict and Harmony of Relations Based on The Little Prince

The Little Prince created by French aristocrat,writer and poet Antoine de Saint–Exupéry not only permeates French romanticism in the 19^(th) century,but implies philosophy of life.As one of the most popular books in the world,it shows that adults or modern people should learn from children to keep kind,imagitive,faithful and so on instead of being worldly-wise,and expresses sincere feelings based on imaginative descriptions.In addition,the movie version with new elements directed by Mark Osborne conquered the world again from a new perspective.And then,a kind of conflicting or harmonious relation is revealed no matter in the original work or in the movie version.However,today’s relationships between people tend to be more complex and less pure since the development of science and technology,artificial intelligence and We Media have become all the rage.Thus,it focuses on revealing the conflict and harmony of individuals’relations through these images in the original work and through a couple of new elements in the 21^(st) century in the movie version to show the transformation from the conflict to harmony.

Psychological Contract within Labor Relation and Its Management Strategy

The paper argues that labor contracts between employees and organization includes two facets, i.e. economic contracts and psychological contracts, and along with enterprise competition becoming fierce increasingly and labor force becoming diversified all the more, the psychological contracts have crucial effects on employees＇ attitudes, behaviors and performances. We also argue that, under the challenging organization context, if managers want to make strategic partnership with employees, they must link employees with hearts, pay attention to the management of psychological contracts before, during and after signing labor contracts, and keep dynamic equilibrium of psychological contracts.

Relative Universality of Human Rights: Theory and Practice

Introduction After the birth of the Universal Declaration of Human Rights （UDHR）, ＂universal human rights＂ became a novel formal legal term both in the field of jurisprudence and in the international community. The notion of ＂universal human rights＂ indicates the ideal of ＂all human rights for all.＂ As the provision in Article 2 of the UDHR stated： ＂Everyone is entitled to all the rights and freedoms set forth in this Declaration, without distinction of any kind, such as race, color, sex, language, religion, politi cal or other opinion, national or social origin, property, birth or other status.＂ However, some nonWestern govern ment officials and intellectuals have expressed doubts about the justifi ability of the term, which seems only to be a reflection of unique Western culture rather than Asian or Arabian ones. At the Vienna World Human Rights Conference, many heads of state said that universal human rights cannot be compatible with nonWest em culture. Even Lee Kuan Yew, the former leader of Singapore, declared in 1996： ＂Asian values are universal values. European values are European values.＇＇~ This statement is obviously a rejection of the idea that human rights are universal. In contrast, universal rights extremists and human rights activists and NGOs, such as Amnesty International and Human Rights Watch, try to propagandize unified international human rights standards and international human rights re gimes. The debate continues from the diplomatic field to the academic field. But, in the foreseeable future,

Antitumor effect of tumor necrosis factor-related apoptosis inducing ligand combined with mevastatin on a human glioma cell line SWO-38

BACKGROUND： Previous studies have reported that statins are less toxic to the human body and have greater antitumor activity; however, few studies have addressed the antitumor effect of statins combined with tumor necrosis factor-related apoptosis inducing ligand （TRAIL）. OBJECTIVE： To explore the effect of TRAIL combined with mevastatin on the proliferation and apoptotic cell death of a human glioma cell line SWO-38, and to study its mechanism of action. DESIGN, TIME AND SETTING： An in vitro control experiment was performed at the Central Laboratory of the Third Hospital Affiliated to Sun Yat-sen University, between January and April 2009. MATERIALS： The human SWO-38 cell line was provided by Cell Research, Department of Animal Experimental Center of Sun Yat-sen University; human recombinant soluble TRAIL by R＆D, USA; and mevastatin by Sigma, USA. METHODS： SWO-38 cells were separately incubated in TRAIL （100, 200, 300, 400, and 500 tJg/L） and mevastatin （5, 10, 20, 30, and 40 pmol/L） for 72 hours. In addition, SWO-38 cells were incubated in TRAIL （300 μg/L）, mevastatin （30 μmol/L）, and a solution containing both TRAIL and mevastatin for 12, 24, 48 and 72 hours. MAIN OUTCOME MEASURES： Cell proliferation was detected using methyl thiazolyl tetrazolium assay; cell apoptosis was observed using Hoechst 33258 staining and fluorescence microscopy and was measured using Annexin V/propidium iodide flow cytometry; TRAIL R1/DR4 and TRAIL R2/DR5 protein expressions levels were measured using indirect immunofluorescence staining combined with flow cytometry in the recombinant soluble TRAIL （rsTRAIL, 300 tJg/L）, mevastatin （30 IJmol/L） and combination groups; TRAIL R1/DR4 and TRAIL R2/DR5 mRNA expression was detected using real-time polymerase chain reaction. RESULTS： rsTRAIL, mevastatin and their combination inhibited tumor proliferation in a time- and dose-dependent manner. The proliferation inhibitory rate and apoptosis rate of human SWO-38 cells in the combined group were significantly greater than the rsTRAIL or mevastatin alone group （P 〈 0.01）. TRAIL R1/DR4 and TRAIL R2/DR5 protein and mRNA expressions were increased in the combination group compared with mevastatin or rsTRAIL alone after 72 hours （P 〈 0.01）. CONCLUSION： Both rsTRAIL and mevastatin inhibit the proliferation and apoptosis of the human glioma cell line SWO-38, while their combination enhances the anti-tumor effect. The mechanism of action possibly correlates to the upregulation of TRAIL R1/DR4 and TRAIL R2/DR5 mRNA expression by mevastatin, thereby enhancing the cell sensitivity to rsTRAIL.