Laser capture microdissection: from its principle to applications in research on neurodegeneration

History and the principle of laser capture microdissection （LCM）： LCM, also known as laser microdissection and pressure catapulting, is one of the most powerful and useful techniques in various research areas where isolation of heterogeneous cell population is required. The first use of laser as a cell operation method, was originated in early 1920s （Gilbrich-Wille, 2013）, and it became widely used as a microsurgical tool ill early 1960s. In the need of extremely small tissue isolation, laser beam of LCM has been modified and developed over several decades from Ultraviolet （UV） laser beam to high-energy nitrogen, infrared and carbon dioxide lasers （Gilbrich-Wille,

The ethics of exercise in eating disorders:Can an ethical principles approach guide the next generation of research and clinical practice?

1.Introduction Eating disorders(ED)are the most common psychiatric disorders afflicting young women^1 and contribute to great detriments in psychological,social,and physical health.^(2,3)Unfortunately,ED treatments tend to be long lasting,intensive,and expensive.~4Additionally,individuals with ED seeking treatment use healthcare services more frequently than non-ED individu-

World Medical Association Declaration of HelsinkiE thical Principles for Medical Research Involving Human Subjects

Document Of World Medical AssociationWorld medical association declaration of Helsinki Ethical principles for medical research involving human subjects

Theoretical Characteristics of Tibetan Medicine

Similar to traditional Chinese medicine(TCM),Tibetan medicine emphasizes the concepts of holism,balance,and etiology.With the individual as the nexus of macro-and micro-ecologies,multi-layered systemic etiologies,inform therapeutic pharmacologies for cultivating network effects to induce the healing response.Tibetan medicine approaches health and healing through perspectives that frame the balance achieved in the internal world as inextricably linked to balance in the external world,and vice versa,that includes expressions of mind and consciousness.The external world refers to the broader context of the natural world as comprised of matter and energy expressed through five elemental dynamics(Chi.Tu,Shui,Huo,Feng and Kong;Tib.Sa,Chu,Mé,r Lung and Kha)describing principles of solidity,cohesion,heat,motility,and interactive space,or earth,water,fire,wind and space,respectively.The internal world refers to the local context of the physical mass and biochemical cascades of energy transfers comprising the human body,its behaviors and functions,including cognitive factors,as also expressed through the five elemental dynamics.The external and internal systems merge as a unified whole,where the five elemental dynamics characterize materio-energetic exchanges between the broader and local ecologies of individual body and natural world,as well as social relations,mental affects,and contextual conditions.The interdependence,opposition,restriction and transformation among three physiological systems known as nyépa(Three Causal Factors)-r Lung,Tripa and Béken(pronounced lōōng,trīpa,baekan,respectively)-are used to discuss the balance of the whole.The causal origins of disease are explained through disturbances to these systems as a combination of afflictive mental factors,physiologic imbalances,and disturbing conditions from the natural world,such as environmental toxins,pathogens,and exposures.Of the three causal origins,afflictive mental factors are particularly emphasized as important etiological conditioning influences.Initiation and progression of disease is divided into four stages:baseline stage,initiating stage,developing stage,and maturing stage.Each stage has an explicit therapeutic paradigm for treatment,recovery,and health maintenance demonstrating the distinct theoretical framework for disease treatment in Tibetan medicine as specific to individual body,particular disease etiology,and constitutionally-directed unique compound metabolism pharmacodynamically.