Objective： To analyze the changes in peripheral natural killer T-cells （NKT） and gammadelta T-cells （γδT-cell） in patients with minimal residual leukemia （MRL） before and after being treated with Yiqi Bushen ...Objective： To analyze the changes in peripheral natural killer T-cells （NKT） and gammadelta T-cells （γδT-cell） in patients with minimal residual leukemia （MRL） before and after being treated with Yiqi Bushen Granule （益气补肾颗粒, YBG） in order to determine their significance in prognosis of the disease. Methods： Before and after treatment, the changes in 36 patients （16 males and 20 females） receiving long-term （more than 3 months） YBG therapy were analyzed using multi-parameter flow cytometry, with 34 healthy persons （19 males and 15 females） acting as controls. Results： The absolute value and percentage of NKT cells and γδT-cells were all significantly raised after treatment, for NKT cells, 0.52%±0.39% to 0.83%±0.66% and 7.25±7.77 cell/μL to 12.86±11.99 cell/μL, for γδT-cells, 6.08%±3.03% to 7.24%±2.78% and 83.97±48.09 cell/μL to 110.53±54.12 cell/μL, respectively （P0.05 or P0.01）. Conclusion： YBG could regulate the immune function and elevate the amount of NKT cells and γδT-cells, thus to kill or suppress the residual leukemic cell in the body, which might be one of the mechanisms of YBG in prolonging the disease-free survival in MRL patients.展开更多
To observe the effects of Fuzheng Kangbai Granule (FZKBG) on immune function and survival time in minimal residual leukemia (MRL) model mice and explore its mechanism. Methods: MRL model mice were established by hypod...To observe the effects of Fuzheng Kangbai Granule (FZKBG) on immune function and survival time in minimal residual leukemia (MRL) model mice and explore its mechanism. Methods: MRL model mice were established by hypodermic inoculation with L7212 cells 1×106 following intraperitoneal injection of cytoxan (CTX) 250 mg/kg 3 days later, and divided into the control group and FZKBG treated group. The changes of T-lymphocyte subsets, including CD3+, CD4+ and CD8+, and the survival time in model mice were observed. Results: Compared with the control, FZKBG could obviously increase both the percentage and absolute value of CD3+ and CD4+ lymphocytes and prolong the survival time of model mice, the prolongation rate being 29.6% - 60.4%. Conclusion: FZKBG could markedly prolong the survival time of MRL mice, and its mechanism might be through elevating the immunologic function and inhibiting the leukemia cells in model mice.展开更多
基金Supported by the National Natural Science Foundation of China (No. 30472284)the Capital Scientific Research Development Fund for Key Medical Program (TCM, No. I-1)
文摘Objective： To analyze the changes in peripheral natural killer T-cells （NKT） and gammadelta T-cells （γδT-cell） in patients with minimal residual leukemia （MRL） before and after being treated with Yiqi Bushen Granule （益气补肾颗粒, YBG） in order to determine their significance in prognosis of the disease. Methods： Before and after treatment, the changes in 36 patients （16 males and 20 females） receiving long-term （more than 3 months） YBG therapy were analyzed using multi-parameter flow cytometry, with 34 healthy persons （19 males and 15 females） acting as controls. Results： The absolute value and percentage of NKT cells and γδT-cells were all significantly raised after treatment, for NKT cells, 0.52%±0.39% to 0.83%±0.66% and 7.25±7.77 cell/μL to 12.86±11.99 cell/μL, for γδT-cells, 6.08%±3.03% to 7.24%±2.78% and 83.97±48.09 cell/μL to 110.53±54.12 cell/μL, respectively （P0.05 or P0.01）. Conclusion： YBG could regulate the immune function and elevate the amount of NKT cells and γδT-cells, thus to kill or suppress the residual leukemic cell in the body, which might be one of the mechanisms of YBG in prolonging the disease-free survival in MRL patients.
基金State Administration of TCM，the 3rd grade Award of Scientific and Technological Advancement of China Academy of TCM
文摘To observe the effects of Fuzheng Kangbai Granule (FZKBG) on immune function and survival time in minimal residual leukemia (MRL) model mice and explore its mechanism. Methods: MRL model mice were established by hypodermic inoculation with L7212 cells 1×106 following intraperitoneal injection of cytoxan (CTX) 250 mg/kg 3 days later, and divided into the control group and FZKBG treated group. The changes of T-lymphocyte subsets, including CD3+, CD4+ and CD8+, and the survival time in model mice were observed. Results: Compared with the control, FZKBG could obviously increase both the percentage and absolute value of CD3+ and CD4+ lymphocytes and prolong the survival time of model mice, the prolongation rate being 29.6% - 60.4%. Conclusion: FZKBG could markedly prolong the survival time of MRL mice, and its mechanism might be through elevating the immunologic function and inhibiting the leukemia cells in model mice.
