An interspecific hybrid line pf9279 was obtained by proto-plast fusion between 02428(japonica, with a wide com-patibility gene) and CNW240 (O. officinalis, fromMalaysia) at CNRRI in 1992. Possible introgression of
From 1986 to 1993, a set of near-isogenic japonicarice Iines with three major genes Xα-3, Xα - 4,and Xα-12 for resistance to bacterial blight(Xan-thomonas oryzae pv.oryzae)were developed anddesignated as CBB3, CBB4...From 1986 to 1993, a set of near-isogenic japonicarice Iines with three major genes Xα-3, Xα - 4,and Xα-12 for resistance to bacterial blight(Xan-thomonas oryzae pv.oryzae)were developed anddesignated as CBB3, CBB4, and CBB12 respective-展开更多
A set of near-isogenic rice lines withmonogenic resistance to bacterial blight weredeveloped by IRRI.The Cultivar IR24 wasused as the recurred parent.They wereevaluated with 6 races of Xanthomonascampestris pv.oryzae(...A set of near-isogenic rice lines withmonogenic resistance to bacterial blight weredeveloped by IRRI.The Cultivar IR24 wasused as the recurred parent.They wereevaluated with 6 races of Xanthomonascampestris pv.oryzae(Xco)in the Philip-pines at the maximum tillering and the bootingstages by ZHANG and MEW at IRRI in 1989.展开更多
To explore ef fective physiological indexes to distinguish the resistant lines and susceptible lines of Pyt hium porphyrae,the causal agent of red rot disease of Pyropia yezoensis,and establish the disease-resistance ...To explore ef fective physiological indexes to distinguish the resistant lines and susceptible lines of Pyt hium porphyrae,the causal agent of red rot disease of Pyropia yezoensis,and establish the disease-resistance breeding strategy,we obtained and analyzed the candidate resistant and susceptible lines by population selection.Gametophytes of the candidate lines were cultured in seawater containing Pyt.porphyrae zoospores.Antioxidase activities,including superoxide dismutase(SOD),peroxidase(POD)and polyphenol oxidase(PPO),were measured and compared between the two lines before and after infection.In the resistant lines,SOD and POD activities increased and then decreased,but PPO activity rose with the prolongation of the infection time.The phenylalanine ammonia lyase(PAL)activities also increased and then decreased after infection,but it had significantly different expression in the two lines without pathogen attack.The synthesis rates ofβ-1,3-glucanase,and cell-wall degrading enzyme were different from each other between the two lines after infection of P yt.porphyrae.Comparison in the contents of malondialdehyde(MDA)and reactive oxygen species(ROS)in the two lines showed that,the two contents varied synchronously in response to the pathogen attack.Changes of these enzymes activities or contents demonstrated that Pyr.yezoensis could resist against the pathogen of P yt.porphyrae with the antioxidant defense capacity.In addition,β-1.3-glucanase content showed extremely significant dif ference between the two lines,and the PAL had consistent expression dif ference.Therefore,phenylalanine ammonia lyase(PAL)andβ-1,3-glucanase can be considered as an ef fective index to distinguish susceptible line and resistant line,with which the workload of the resistant breeding could be reduced in the future.展开更多
An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onoblychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2, 4-dichlorophenoxy-acetic acid...An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onoblychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2, 4-dichlorophenoxy-acetic acid (2,4-D), 0.5 mg/L kinetin (KT) and 0.2 mg/L naphthalene acetic acid (NAA), the division frequency of protoplastderived cells reached uP to over 60 %, and microcalli were obtained in 5-6 wk. Upon transferring them on agar solidified MS medium plus 2 mg/L indole-3-acetic acid (IAA), shoots were induced. After cultivating them on MS medium with or without IAA, roots were regenerated.Chromosome number of all protoplast-regenerated plants examined were normal (2n=28). The protoplast-derived calli and plants grew vigorously on the medium containing 10 mmol/L HYP.展开更多
Ug99, also designated as TFKSK, is a race of Puccinia graminis Pers.:Pers f. sp. tn'tici Eriks. and E. Henn (Pgt) with broad virulence to wheat. It is the first known Pgt race possessing virulence to Sr31, a stem ...Ug99, also designated as TFKSK, is a race of Puccinia graminis Pers.:Pers f. sp. tn'tici Eriks. and E. Henn (Pgt) with broad virulence to wheat. It is the first known Pgt race possessing virulence to Sr31, a stem rust resistance (Sr) gene deployed in wheat varieties world- wide (Singh et al., 2011 ). Since the first detection of TFKSK in 1998, a total of 13 Ug99 variants have been identified in several African countries.展开更多
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widely distributed and destructive fungal diseases worldwide. Since 1995, most Chinese wheat cultivars have lost their stripe rust r...Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widely distributed and destructive fungal diseases worldwide. Since 1995, most Chinese wheat cultivars have lost their stripe rust resistance due to the subsequent emergence of the new races CYR30, CYR31, CYR32, and CYR33 (Han et al., 2010). Therefore, it is necessary to seek effective resistance genes and develop new resistance germ- plasm for wheat resistance breeding.展开更多
Wide hybridization is an effective approach for enhancing the resistance of bread wheat (Triticum aestivum L.) to biotic and abiotic stresses by introducing favorable alien genes (Sepsi et al., 2008). Wheatgrass, ...Wide hybridization is an effective approach for enhancing the resistance of bread wheat (Triticum aestivum L.) to biotic and abiotic stresses by introducing favorable alien genes (Sepsi et al., 2008). Wheatgrass, Thinopyrum intermedium (Host) Barkworth & D.R. Dewey or Agropyron intermedium (Host) Beauvoir (2n = 42; genome formula JJjSjSstst), is a perennial species in the tribe Triticeae and an important source of wheat improvement for biotic and abiotic stress resistance and quality-related traits, such as high grain protein concentration (Chen et al., 1998; 2001; 2003; Han et al., 2004; Li and Wang, 2009). In addition, the ready crossing ability of wheatgrass with various Triticum species has made it popular in germ- plasm development.展开更多
The need to have naval units ever faster pushed the ship design to design hull shapes with increasingly higher performance thanks to the use of lightweight materials such as aluminum, and more powerful engines, etc., ...The need to have naval units ever faster pushed the ship design to design hull shapes with increasingly higher performance thanks to the use of lightweight materials such as aluminum, and more powerful engines, etc., but without substantially modifying the traditional forms of hull. The hull patented Monotricat high hydrodynamic efficiency and energy saving it represents an evolution of the traditional architectures of the hulls, as its shape is adapted to recover wave formation engendered from the bow and sprays associated with it so as to reduce the resistance to the benefit of the speed, and navigating in displacement at speeds of planing hulls with an efficiency of about 20%. The patented hull Monotricat represents the overcoming of distinction between displacement and planing hulls, because, unlike previous solutions, the hull conventionally called Monotricat is the first displacement hull that can navigate at both displacement and planning speeds, with a resistance curve almost straight, maintaining the characteristics of a displacement hull, since it combines the characteristics of displacement and planning hull. It presents an innovative architecture that could be defined as a hybrid between a monohull and catamaran, navigating on spray self-produced. The combination of these three types of naval hulls allows it to ensure: safety, comfort navigation, best seakeeping and maneuverability in restricted waters, stability, reduction of resistance to motion, cost management, regularity on the routes even in adverse weather-sea. These characteristics of the hull have been studied, tested and validated by leading research institutes and universities with more ameliorative results in each subsequent experimentation, reported in the present work, which demonstrated a greater hydrodynamic efficiency compared to conventional hulls of 20%.展开更多
The effect of the annealing time and annealing temperature on Ni/Ge/Au electrode contacts deposited on the n-type InP contact layer has been studied using a circular transmission line model. The minimum specific conta...The effect of the annealing time and annealing temperature on Ni/Ge/Au electrode contacts deposited on the n-type InP contact layer has been studied using a circular transmission line model. The minimum specific contact resistance of 3.210 7 cm2was achieved on the low-doped n-type InP contact layer with a 40 s anneal at 425 ℃. In order to improve the ohmic contact and reduce the difficulty in the fabrication of the high doped InP epi-layer, the doping concentration in the InP contact layer was chosen to be 51018cm 3in the fabrication of transferred electronic devices. Excellent differential negative resistance properties were obtained by an electron beam evaporating the Ni/Ge/Au/Ge/Ni/Au composite electrode on an InP epi-layer with a 60 s anneal at 380 ℃.展开更多
文摘An interspecific hybrid line pf9279 was obtained by proto-plast fusion between 02428(japonica, with a wide com-patibility gene) and CNW240 (O. officinalis, fromMalaysia) at CNRRI in 1992. Possible introgression of
文摘From 1986 to 1993, a set of near-isogenic japonicarice Iines with three major genes Xα-3, Xα - 4,and Xα-12 for resistance to bacterial blight(Xan-thomonas oryzae pv.oryzae)were developed anddesignated as CBB3, CBB4, and CBB12 respective-
文摘A set of near-isogenic rice lines withmonogenic resistance to bacterial blight weredeveloped by IRRI.The Cultivar IR24 wasused as the recurred parent.They wereevaluated with 6 races of Xanthomonascampestris pv.oryzae(Xco)in the Philip-pines at the maximum tillering and the bootingstages by ZHANG and MEW at IRRI in 1989.
基金Supported by the National Natural Science Foundation of China(Nos.31372517,41006082)
文摘To explore ef fective physiological indexes to distinguish the resistant lines and susceptible lines of Pyt hium porphyrae,the causal agent of red rot disease of Pyropia yezoensis,and establish the disease-resistance breeding strategy,we obtained and analyzed the candidate resistant and susceptible lines by population selection.Gametophytes of the candidate lines were cultured in seawater containing Pyt.porphyrae zoospores.Antioxidase activities,including superoxide dismutase(SOD),peroxidase(POD)and polyphenol oxidase(PPO),were measured and compared between the two lines before and after infection.In the resistant lines,SOD and POD activities increased and then decreased,but PPO activity rose with the prolongation of the infection time.The phenylalanine ammonia lyase(PAL)activities also increased and then decreased after infection,but it had significantly different expression in the two lines without pathogen attack.The synthesis rates ofβ-1,3-glucanase,and cell-wall degrading enzyme were different from each other between the two lines after infection of P yt.porphyrae.Comparison in the contents of malondialdehyde(MDA)and reactive oxygen species(ROS)in the two lines showed that,the two contents varied synchronously in response to the pathogen attack.Changes of these enzymes activities or contents demonstrated that Pyr.yezoensis could resist against the pathogen of P yt.porphyrae with the antioxidant defense capacity.In addition,β-1.3-glucanase content showed extremely significant dif ference between the two lines,and the PAL had consistent expression dif ference.Therefore,phenylalanine ammonia lyase(PAL)andβ-1,3-glucanase can be considered as an ef fective index to distinguish susceptible line and resistant line,with which the workload of the resistant breeding could be reduced in the future.
文摘An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onoblychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2, 4-dichlorophenoxy-acetic acid (2,4-D), 0.5 mg/L kinetin (KT) and 0.2 mg/L naphthalene acetic acid (NAA), the division frequency of protoplastderived cells reached uP to over 60 %, and microcalli were obtained in 5-6 wk. Upon transferring them on agar solidified MS medium plus 2 mg/L indole-3-acetic acid (IAA), shoots were induced. After cultivating them on MS medium with or without IAA, roots were regenerated.Chromosome number of all protoplast-regenerated plants examined were normal (2n=28). The protoplast-derived calli and plants grew vigorously on the medium containing 10 mmol/L HYP.
基金supported by the Ministry of Science and Technology of China (No. 2014DFA31540)Chinese Academy of Sciences (No. SAJC201305)the Bill & Melinda Gates Foundation to Cornell University for the Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project
文摘Ug99, also designated as TFKSK, is a race of Puccinia graminis Pers.:Pers f. sp. tn'tici Eriks. and E. Henn (Pgt) with broad virulence to wheat. It is the first known Pgt race possessing virulence to Sr31, a stem rust resistance (Sr) gene deployed in wheat varieties world- wide (Singh et al., 2011 ). Since the first detection of TFKSK in 1998, a total of 13 Ug99 variants have been identified in several African countries.
基金supported by the grants from the National High Technology Research and Development Program of China (No. 2011AA100102)the Chinese Academy of Sciences (No. KSCX2-EW-N-02)
文摘Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widely distributed and destructive fungal diseases worldwide. Since 1995, most Chinese wheat cultivars have lost their stripe rust resistance due to the subsequent emergence of the new races CYR30, CYR31, CYR32, and CYR33 (Han et al., 2010). Therefore, it is necessary to seek effective resistance genes and develop new resistance germ- plasm for wheat resistance breeding.
基金supported by the Provincial Prize Fund for Distinguished Young and Middle-aged Scientists of Shandong Province(No.BS2011SW053)State Key Laboratory of Plant Cell and Chromosome Engineering(No.PCCE-KF-2014-01)State Key Laboratory of Crop Biology(No.2015KF06)
文摘Wide hybridization is an effective approach for enhancing the resistance of bread wheat (Triticum aestivum L.) to biotic and abiotic stresses by introducing favorable alien genes (Sepsi et al., 2008). Wheatgrass, Thinopyrum intermedium (Host) Barkworth & D.R. Dewey or Agropyron intermedium (Host) Beauvoir (2n = 42; genome formula JJjSjSstst), is a perennial species in the tribe Triticeae and an important source of wheat improvement for biotic and abiotic stress resistance and quality-related traits, such as high grain protein concentration (Chen et al., 1998; 2001; 2003; Han et al., 2004; Li and Wang, 2009). In addition, the ready crossing ability of wheatgrass with various Triticum species has made it popular in germ- plasm development.
文摘The need to have naval units ever faster pushed the ship design to design hull shapes with increasingly higher performance thanks to the use of lightweight materials such as aluminum, and more powerful engines, etc., but without substantially modifying the traditional forms of hull. The hull patented Monotricat high hydrodynamic efficiency and energy saving it represents an evolution of the traditional architectures of the hulls, as its shape is adapted to recover wave formation engendered from the bow and sprays associated with it so as to reduce the resistance to the benefit of the speed, and navigating in displacement at speeds of planing hulls with an efficiency of about 20%. The patented hull Monotricat represents the overcoming of distinction between displacement and planing hulls, because, unlike previous solutions, the hull conventionally called Monotricat is the first displacement hull that can navigate at both displacement and planning speeds, with a resistance curve almost straight, maintaining the characteristics of a displacement hull, since it combines the characteristics of displacement and planning hull. It presents an innovative architecture that could be defined as a hybrid between a monohull and catamaran, navigating on spray self-produced. The combination of these three types of naval hulls allows it to ensure: safety, comfort navigation, best seakeeping and maneuverability in restricted waters, stability, reduction of resistance to motion, cost management, regularity on the routes even in adverse weather-sea. These characteristics of the hull have been studied, tested and validated by leading research institutes and universities with more ameliorative results in each subsequent experimentation, reported in the present work, which demonstrated a greater hydrodynamic efficiency compared to conventional hulls of 20%.
基金Project supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(No.YYYJ1123)
文摘The effect of the annealing time and annealing temperature on Ni/Ge/Au electrode contacts deposited on the n-type InP contact layer has been studied using a circular transmission line model. The minimum specific contact resistance of 3.210 7 cm2was achieved on the low-doped n-type InP contact layer with a 40 s anneal at 425 ℃. In order to improve the ohmic contact and reduce the difficulty in the fabrication of the high doped InP epi-layer, the doping concentration in the InP contact layer was chosen to be 51018cm 3in the fabrication of transferred electronic devices. Excellent differential negative resistance properties were obtained by an electron beam evaporating the Ni/Ge/Au/Ge/Ni/Au composite electrode on an InP epi-layer with a 60 s anneal at 380 ℃.