Enhancing Accumulation and Penetration Efficiency of Next-Generation Antibiotics to Mitigate Antibiotic Resistance in Pseudomonas aeruginosa PAO1

This study explores the efficacy of advanced antibiotic compounds against P. aeruginosa, focusing on Antibiotic B, an enhanced derivative of Ceftriaxone. The study measured the intracellular uptake of Antibiotic B and introduced a novel adjuvant, Influximax, which augmented its antibacterial activity. Results showed a diminished potential for resistance emergence with Antibiotic B, particularly when used in combination with Influximax. The study suggests that optimizing antibiotic delivery into bacterial cells and leveraging syner-gistic adjuvant combinations can enhance drug resistance combat. .

Obtaining 2D Soil Resistance Profiles from the Integration of Electrical Resistivity Data and Standard Penetration Test (SPT) and Light Dynamic Penetrometer (DPL) Resistance Tests—Applications in Mass Movements Studies

In Brazil and various regions globally, the initiation of landslides is frequently associated with rainfall;yet the spatial arrangement of geological structures and stratification considerably influences landslide occurrences. The multifaceted nature of these influences makes the surveillance of mass movements a highly intricate task, requiring an understanding of numerous interdependent variables. Recent years have seen an emergence in scholarly research aimed at integrating geophysical and geotechnical methodologies. The conjoint examination of geophysical and geotechnical data offers an enhanced perspective into subsurface structures. Within this work, a methodology is proposed for the synchronous analysis of electrical resistivity geophysical data and geotechnical data, specifically those extracted from the Light Dynamic Penetrometer (DPL) and Standard Penetration Test (SPT). This study involved a linear fitting process to correlate resistivity with N10/SPT N-values from DPL/SPT soundings, culminating in a 2D profile of N10/SPT N-values predicated on electrical profiles. The findings of this research furnish invaluable insights into slope stability by allowing for a two-dimensional representation of penetration resistance properties. Through the synthesis of geophysical and geotechnical data, this project aims to augment the comprehension of subsurface conditions, with potential implications for refining landslide risk evaluations. This endeavor offers insight into the formulation of more effective and precise slope management protocols and disaster prevention strategies.

Resistance Factor of Caisson-Tip with Internal Fillet for Suction Caissons Penetrating into Clay

This paper presents failure mechanisms of the soil at the caisson-tip with fillet during suction-assisted penetration of suction caissons in undrained clay.Theoretical solutions of resistance factor N_c of the caisson-tip are obtained in terms of the caisson-tip geometry ratio of the flat section of the caisson-tip to the caisson wall thickness m/t and adhesion factorsα_i along inside of caisson wall andα_b at the base of the caisson-tip.It is indicated that the factor N_c increases with the increase of m/t,α_i and a_b.The resistance factors N_c for the rough base(α_b=1)are larger by 0.57than that for the smooth base(α_b=0).Besides,the factors N_c of caisson-tip with flat base(m=t)are larger by 1.14 than that with full internal fillet(m=0).The required suction to penetrate suction caissons with various fillets is obtained in terms of the force equilibrium in vertical direction.The finite element limit analysis and centrifuge model test results are used to verify the rationality of the presented failure mechanisms and theoretical predictions.

Effects of material of metallic frame on the penetration resistances of ceramic-metal hybrid structures

The effects of metallic material on the penetration resistances of ceramic-metal hybrid structures against vertical long-rod tungsten projectiles were studied by artillery-launched experiments and numerical simulation.Hybrid structures with rectangular cores in transverse orthogonal arrangement and slidefitting ceramic inserts of zirconia toughened alumina prisms were fabricated with titanium alloy TC4(Ti6 Al4 V),AISI 4340 steel and 7075 aluminum alloy panels,respectively.The results showed that the hybrid structure of Ti6A14V exhibited the highest penetration resistance,followed by that of 7075 aluminum alloy with the same area density.The penetration resistance of the hybrid structure of AISI4340 steel was the lowest.The underlying mechanisms showed that the metallic material of a ceramicmetal hybrid structure can directly affect its energy absorption from the impact projectile,which further affects its penetration resistance.Different metallic frames exhibited different failure characteristics,resulting in different constraint conditions or support conditions for ceramic prisms.The high penetration resistance of the Ti6Al4V hybrid structure was due to its stronger back support to ceramic prisms as compared with that of AISI 4340 steel hybrid structure,and better constraint condition for ceramic prisms by metallic webs as compared with that of 7075 aluminum alloy hybrid structure.The results of mass efficiency and thickness efficiency showed that the Ti6Al4V hybrid structure has advantages in reducing both the thickness and the mass of protective structure.In addition,because the ceramic-metal hybrid structures in the present work were heterogeneous,impact position has slight influence on their penetration resistances.

Penetration and impact resistance of PDC cutters inclined at different attack angles

In order to develop a rotary percussive bit with diamond enhanced cutters assisted by high pressure water jets, it is necessary to study the damage mechanism and the penetration properties of PDC cutters subject to different impact load level and rock types. Therefore the impact experiments of the single PDC cutters with different attack angles in four rocks: black basalt, Missouri red granite, Halston limestone, and a very soft (Roubidoux) sandstone were carried out, and the effects of rake angles of PDC cutters on both the penetration and impact resistance of PDC cutters have been discussed in detail. Test results show that a PDC insert can withstand a very strong impact in compression but is easily damaged by impact shearing, the PDC cutters are more easily damaged by shearing if the attack angles are relatively small, the 45? PDC cutters have the least penetration resistance among the cutters tested. Thus it is suggested that the attack angles of PDC cutters should be larger than 30? for bits which must withstand impact from a hammer.

Penetration Resistance of Skirt-Tip with Rough Base for Suction Caissons in Clay

Suction caissons can readily penetrate into the seabed under the combination of the self-weight and suction resulted from the encased water being increasingly pumped out. During suction-assisted penetration, the equivalent overburden at the skirt-tip level outside the caisson is generally higher than that inside because the vertical stress within the soil plug is reduced by the exerted suction. This may result in a uniform shear stress developing over the base of the skirt-tip as the soil below the skirt-tip tends to move into the caisson, which leads to an asymmetric failure wedge existing below the base of the skirt-tip. Besides, different adhesion factors along the inside(αi) and outside(αo) of the skirt wall will cause asymmetric plastic zones inside and outside the caisson. Accordingly, an asymmetric failure mechanism is therefore proposed to calculate the penetration resistance of the skirt-tip. The proposed failure mechanism is the first to consider the effect of different adhesion factors(αi) and(αo) on the failure mechanism at the skirt-tip, and involves the contribution from the weighted average of equivalent overburdens inside and outside caisson at the skirt-tip level. The required suction pressure can be obtained in terms of force equilibrium of the caisson in a vertical direction. Finally, the asymmetric failure mechanism at the skirt-tip is validated with the FE calculations. By comparing with the measured data, the predictions of the required suction pressure are found to be in good agreement with the experimental results.

Fatigue Resistance and Failure Behavior of Penetration and Non-Penetration Laser Welded Lap Joints

Penetration and non-penetration lap laser welding is the joining method for assembling side facade panels of railway passenger cars,while their fatigue performances and the difference between them are not completely understood.In this study,the fatigue resistance and failure behavior of penetration 1.5+0.8-P and non-penetration 0.8+1.5-N laser welded lap joints prepared with 0.8 mm and 1.5 mm cold-rolled 301L plates were investigated.The weld beads showed a solidification microstructure of primary ferrite with good thermal cracking resistance,and their hardness was lower than that of the plates.The 1.5+0.8-P joint exhibited better fatigue resistance to low stress amplitudes,whereas the 0.8+1.5-N joint showed greater resistance to high stress amplitudes.The failure modes of 0.8+1.5-N and 1.5+0.8-P joints were 1.5 mm and 0.8 mm lower lap plate fracture,respectively,and the primary cracks were initiated at welding fusion lines on the lap surface.There were long plastic ribs on the penetration plate fracture,but not on the non-penetration plate fracture.The fatigue resistance stresses in the crack initiation area of the penetration and non-penetration plates calculated based on the mean fatigue limits are 408 MPa and 326 MPa,respectively,which can be used as reference stress for the fatigue design of the laser welded structures.The main reason for the difference in fatigue performance between the two laser welded joints was that the asymmetrical heating in the non-penetration plate thickness resulted in higher residual stress near the welding fusion line.

Influence of thickness and projectile shape on penetration resistance of the compliant composite

The present study deals with development of conceptual proof for jute rubber based flexible composite block to completely arrest the projectile impacting the target at high velocity impact of 400 m/s through numerical simulation approach using finite element(FE)method.The proposed flexible composite blocks of repeating jute/rubber/jute(JRJ)units are modelled with varying thickness from 30 mm to 120 mm in increments of 30 mm and impacted by flat(F),ogival(O)and hemispherical(HS)shaped projectiles.All the considered projectiles are impacted with proposed flexible composite blocks of different thicknesses and the penetration behaviour of the projectile in each case is studied.The penetration depth of the projectile in case of partially penetrated cases are considered and the effect of thickness and projectile shape on percentage of penetration depth is statistically analyzed using Taguchi’s design of experiments(DOE).Results reveal that the though proposed flexible composite block with thickness of 90 mm is just sufficient to arrest the complete penetration of the projectile,considering the safety issues,it is recommended to use the flexible composite with thickness of 120 mm.The nature of damage caused by the projectile in the flexible composite is also studied.Statistical studies show that thickness of the block plays a prominent role in determining the damage resistance of the flexible composite.

Undue Hardness/Modulus Ratio Claims instead of Physical Penetration Resistance and Applications with Mollusk Shells

The Nanoindentation is a precise technique for the elucidation of mechanical properties. But such elucidation requires physically based interpretation of the loading curves that is widely still not practiced. The use of indentation hardness H and indentation modulus Er is unphysical and cannot detect the most important phase-transitions under load that very often occur. The claim that H versus E plots relate linearly for all different materials is neither empirically found nor correctly deduced. It is most dangerous by producing incorrect materials properties and misleading. The use of H/E (that is also called “elasticity index”) in complicated formulas for brittle parameter, yield strength, toughness, and so-called “true hardness” is also in error. The use of H/E cannot reveal the true qualities of materials without considering phase-transitions under load that require the correct exponent 3/2 on h for the loading curves (instead of disproved 2). This is exemplified with the physical data of different mollusk shells that experience phase-transitions, a new bionics model, and different contributions for their strengthening. The data are compared to the ones of aragonite and calcite and vaterite.

Penetration Resistance of Composite Bucket Foundation with Eccentric Load for Offshore Wind Turbines

The penetration of the composite bucket foundation(CBF)is crucial in its construction process.In actual projects,the foundation is inevitably subjected to eccentric load caused by towers and turbines,as well as wind,wave,and flow,during the one-step installation.Moreover,the eccentric load is bound to affect the penetration method and penetration resistance of the foundation.To examine the above-mentioned issues,the penetration resistance of CBF with eccentric load was calculated and analyzed based on model tests,and the seepage field of the CBF under eccentric load was analyzed using ABAQUS.The influence of different magnitudes of eccentric load and various offset strategies on penetration resistance was analyzed,and the theoretical and measured values were compared.The result indicated that the negative pressure of the offset room was found to be smaller than that of other rooms when the CBF penetrated the soil under eccentric load.The penetration resistance of CBF under eccentric load was larger than that without eccentricity,and the larger the eccentric load is,the greater the penetration resistance.The influence of different eccentric load offset strategies on penetration resistance was found to be negligible.The calculated penetration resistance under eccentric load was in good agreement with the measured value.

Evaluation of Shear Strength and Cone Penetration Resistance Behavior of Tropical Silt Loam Soil under Uni-Axial Compression

Laboratory investigations were conducted to study strength characteristics of silt loam soil of Ilorin, Kwara State, Nigeria, under uni-axial compression tests. The main objective of this study was to evaluate the effects of applied pressure and moisture content on strength indices such as bulk density, penetration resistance and shear strength of the soil and to develop relationships between the strength indices for predictive purposes necessary in soil management. The compression was carried out at different moisture contents determined according to the consistency limits of the soil. The applied pressure ranged from 75 to 600 kPa. Values of bulk density, penetration resistance and shear strength increased with increase in moisture content up to peak values after which the values decreased with further increase in moisture content. Regression models were used to describe the trends in the results for the soil. Results also showed that bulk density and soil strength normally regarded as indicators of soil quality are affected by moisture content and applied pressure and that these properties can be predicted using the models generated from the study.

Relating Cone Penetration and Rutting Resistance to Variations in Forest Soil Properties and Daily Moisture Fluctuations

Soil resistance to penetration and rutting depends on variations in soil texture, density and weather-affected changes in moisture content. It is therefore difficult to know when and where off-road traffic could lead to rutting-induced soil disturbances. To establish some of the empirical means needed to enable the “when” and “where” determinations, an effort was made to model the soil resistance to penetration over time for three contrasting forest locations in Fredericton, New Brunswick: a loam and a clay loam on ablation/ basal till, and a sandy loam on alluvium. Measurements were taken manually with a soil moisture probe and a cone penetrometer from spring to fall at weekly intervals. Soil moisture was measured at 7.5 cm soil depth, and modelled at 15, 30, 45 and 60 cm depth using the Forest Hydrology Model (ForHyM). Cone penetration in the form of the cone index (CI) was determined at the same depths. These determinations were not only correlated with measured soil moisture but were also affected by soil density (or pore space), texture, and coarse fragment and organic matter content (R2 = 0.54;all locations and soil depths). The resulting regression-derived CI model was used to emulate how CI would generally change at each of the three locations based on daily weather records for rain, snow, and air temperature. This was done through location-initialized and calibrated hydrological and geospatial modelling. For practical interpretation purposes, the resulting CI projections were transformed into rut-depth estimates regarding multi-pass off-road all-terrain vehicle traffic.

Extracellular and intracellular infection of Botryosphaeria dothidea and resistance mechanism in apple cells

Apple ring rot,which is caused by Botryosphaeria dothidea,severely affects apple production.The mechanisms employed in apple cells against B.dothidea remain unknown.In this research,the pathogen infection mode and the relationship between cell death and disease resistance in‘Fuji’/B.dothidea interaction pathosystem were investigated.By using transmission electron microscopy(TEM),our research showed that the pathogen infects apple cells both intracellularly and extracellularly.However,compared with that in immature fruit,the incidence of hyphae in the interior of mature apple fruit cells increased dramatically,suggesting that cell wall-mediated penetration resistance could be important in apple resistance against B.dothidea.TEM ultrastructural characterization identified the nuclear morphology of programmed cell death induction in both apple fruit and callus cells under B.dothidea infection.Overexpression of MdVDAC2(MDP0000271281),which encodes an outer-membrane localized anion channel protein in mitochondria,significantly promoted cell death under B.dothidea infection and simultaneously inhibited pathogen infection,suggesting that cell death represents a disease resistance mechanism in apple against B.dothidea infection.Furthermore,BdCatalase(KAF4307763),a cytochromeP450 family protein BdCYP52A4(KAF4300696),and subtilisin-domain containing proteinswere identified fromB.dothidea-secreted proteins,which suggested the potential involvement of active oxygen species and phytoalexins in combating B.dothidea infection and triggering or dampening apple resistance.Collectively,our research suggested that cell wall-mediated penetration resistance,programmed cell death machinery and microbial effector-interrelated signaling were among strategies recruited in apple to combat B.dothidea.The current research laid the foundation for further investigations into resistance mechanisms in apple.

Penetration Tests of Multi-Bucket Foundation in Sand for Offshore Wind Turbines

Suction foundations are generally installed with negative pressures to overcome the resistance of soils and complete the penetration,but excessive negative pressures are also avoided to cause seepage damages.In this paper,the model test method was used to analyze the movement characteristics of multi-bucket foundations in the process of sinking in sand,and the common calculation methods of sinking resistances are verified.The critical negative pressure corresponding to the seepage failure of foundation was determined under the action of increasing negative pressure step by step and the characteristics of soil failure were studied.The calculation formula of critical suction in sand was verified in application,and according to the test results,the value of seepage coefficient was modified,which provides an example for the study of suction foundation in sand soils.

Effect of Isobutyl-triethoxy-silane Penetrative Protective Agent on the Carbonation Resistance of Concrete

Effect of isobutyl-triethoxy-silane penetrative protective agent on the carbonation resistance of the concrete was studied.The concrete specimens for the 28 d accelerated carbonation process were manufactured with w/c of 0.49 and 0.64,both in the presence and absence of silane and mineral admixture.The penetration of isobutyl-triethoxy-silane and the carbonation of concrete were investigated by penetration depth,carbonation depth,XRD,SEM,and pore size distribution.The results showed that concrete compactness played an important role in the silane penetration and carbonation resistance.Penetration depth of silane-treated concrete mainly depended on the compactness of the concrete,and could not remarkably change through the accelerated carbonation process.In the accelerated carbonation process,penetrative protective agent improved the carbonation resistance of the higher compactness concretes but accelerated the carbonization process of the lower compactness concretes.As penetrative protective agent penetrated along the external connectivity pores into concrete not filling the entire surface area,the inorganic film could not fully protect the Ca（OH）_2 phase from carbonation.After 28 d accelerated carbonation,fibrous hydration products disappeared and the surface holes decreased.Due to the formation of carbonized products,the porosity of the concrete surface decreased,especially in high-strength concrete.

Numerical study of size effect in concrete penetration with LDPM

Projectile size effect is of great importance since the scaling researches are extensively applied to concrete penetration investigations. This paper numerically deals with the projectile size effect on penetration resistance via the recently developed Lattice Discrete Particles Model(LDPM) which is featured with mesoscale constitutive laws governing the interaction between adjacent particles to account for cohesive fracture, strain hardening in compression and compaction due to pore collapse. Simulations of two different penetration tests are carried to shed some light on the size effect issue. The penetration numerical model is validated by matching the projectile deceleration curve of and predicting the depth of penetration(DOP). By constant velocity penetration simulations, the target resistance is found to be dependent on the projectile size. By best fitting numerical results of constant velocity penetration, a size effect law for target resistance is proposed and validated against literature data. Moreover, the size effect is numerically obtained in the projectile with longer extended nose part meanwhile the shorter extended nose is found to improve the DOP since the projectile nose is sharpened.

Spherical cavity-expansion model for penetration of reinforced-concrete targets

The feature of reinforcing bars is introduced into dynamic cavity-expansion theory. Based on the elastic-plastic response penetration model of plain (i.e., unreinforced) concrete (Forrestal and Tzou, 1997), a dynamic spherical cavity-expansion penetration model for reinforced-concrete targets is developed with consideration of the circumferential restriction effect derived from reinforcing bars in the crushed region. The theoretical solution and simplified calculation formula for the cavity radial stress in incompressible and compressible reinforced concrete are obtained by introducing a reinforcement ratio as the volume fraction of rebars in the concrete target. A damping function is presented to describe the restriction effect of a single layer of reinforcing bars on the surrounding concrete, thus establishing a model to calculate the penetration resistance of multilayer reinforced-concrete targets. Compared with test data for the penetration depth, this model considering the circumferential restriction effect produces better results compared with the existing theory.

Prediction Methods of Spudcan Penetration for Jack-up Units

Jack-up units are extensively playing a successful role in drilling engineering around the world, and their safety and efficiency take more and more attraction in both research and engineering practice. An accurate prediction of the spudcan penetration depth is quite instrumental in deciding on whether a jack-up unit is feasible to operate at the site. The prediction of a too large penetration depth may lead to the hesitation or even rejection of a site due to potential difficulties in the subsequent extraction process; the same is true of a too small depth prediction due to the problem of possible instability during operation. However, a deviation between predictive results and final field data usually exists, especially when a strong-over-soft soil is included in the strata. The ultimate decision sometimes to a great extent depends on the practical experience, not the predictive results given by the guideline. It is somewhat risky, but no choice. Therefore, a feasible predictive method for the spudcan penetration depth, especially in strata with strong-over-soft soil profile, is urgently needed by the jack-up industry. In view of this, a comprehensive investigation on methods of predicting spudcan penetration is executed. For types of different soil profiles, predictive methods for spudcan penetration depth are proposed, and the corresponding experiment is also conducted to validate these methods. In addition, to further verify the feasibility of the proposed methods, a practical engineering case encountered in the South China Sea is also presented, and the corresponding numerical and experimental results are also presented and discussed.

Centrifuge experiment on the penetration test for evaluating undrained strength of deep-sea surface soils

Rapid advances in deep-sea mining engineering have created an urgent need for the accurate evaluation of the undrained strength of marine soils,especially surface soils.Significant achievements have been made using full-flow penetration penetrometers to evaluate marine soil strength in the deep penetration;however,a method considering the effect of ambient water on the surface penetration needs to be established urgently.In this study,penetrometers with multiple probes were developed and used to conduct centrifuge experiments on South China Sea soil and kaolin clay.First,the forces on the probes throughout the penetration process were systematically analyzed and quantified.Second,the spatial influence zone was determined by capturing the resistance changes and sample crack development,and the penetration depth for a sample to reach a stable failure mode was given.Third,the vane shear strength was used to invert the penetration resistance factor of the ball and determine the range of the penetration resistance factor values.Furthermore,a methodology to determine the penetration resistance factors for surface marine soils was established.Finally,the effect of the water cavity above various probes in the surface penetration was used to formulate an internal mechanism for variations in the penetration resistance factor.