Fluorescence lifetime provides a third independent dimension of information for the reso-lution of total luminescence spectra of multicomponent mixtures. The incorporation of this pa-rameter into the Excitation-Emissi...Fluorescence lifetime provides a third independent dimension of information for the reso-lution of total luminescence spectra of multicomponent mixtures. The incorporation of this pa-rameter into the Excitation-Emission Matrix (EEM) by the phase-modulation technique resultsin a three-dimensional Excitation-Emission-Frequency Array (EEFA). Multicomponent analysisbased on the three-dimensional EEFA brings a qualitative change for the resolved spectra, i.e.,individual spectra can be uniquely resolved, which is impossible with any two-dimensional anal-ysis. In this paper, we present a method for analyzing the EEFA. We show mathematicallythat with the three-dimensional analysis of the EEFA individual spectra and lifetimes can be ob-tained. Our algorithm is developed in mathematical detail and demonstrated by its applicationto a two-component mixture.展开更多
文摘Fluorescence lifetime provides a third independent dimension of information for the reso-lution of total luminescence spectra of multicomponent mixtures. The incorporation of this pa-rameter into the Excitation-Emission Matrix (EEM) by the phase-modulation technique resultsin a three-dimensional Excitation-Emission-Frequency Array (EEFA). Multicomponent analysisbased on the three-dimensional EEFA brings a qualitative change for the resolved spectra, i.e.,individual spectra can be uniquely resolved, which is impossible with any two-dimensional anal-ysis. In this paper, we present a method for analyzing the EEFA. We show mathematicallythat with the three-dimensional analysis of the EEFA individual spectra and lifetimes can be ob-tained. Our algorithm is developed in mathematical detail and demonstrated by its applicationto a two-component mixture.
