The Photochemical Study of HSA and BSA with Resonance Light Scattering and Fluorescence Spectra

The resonance light-scattering (RLS) of human serum albumin (HSA) and bovine serum albumin (BSA) is reported for the first time, and applied to study photochemical reaction of HSA and BSA. The fact of photocrosslinking self-association effect in HSA and BSA solutions is identified by the enhancement of RLS. The fluorescence quenching at about 350 nm and 700 nm proves that tryptophan (Trp) residues are one of the photochemical activity sites in HSA and BSA molecules. The Rayleigh scattering (RS) spectra of HSA and BSA that were neglected in fluorescence spectra before are found at about 296 nm, 592 nm and 888 nm for the first time, and are of adventageous to studying the aggregation of HSA or BSA. The possible photochemical reaction mechanism is also proposed.

Effect of dextran molecular weight on resonance light-scattering of quantum dots modified with dextran and concanavalin A

The reversible aggregation between dextran-conjugated CdSe quantum dots（Dex-CdSe-QDs） and concanavalin A（Con A） was explored based on the specific affinity of polysaccharide for Con A by resonance light-scattering technique.In this study,the aggregation of Dex-CdSe-QDs induced by Con A and sequential dissociation by glucose was determined over a wide range of dextran molecular mass（10-500 kDa）.The results demonstrate that the sensitivity of lectin-dextran-modified-QDs interactions increase with dextran molecular mass,and an optimum dextran molecular mass is 500 kDa.

共振光散射比浊法测定胶片废水中银离子的研究

氯化银胶体在375、496和565 nm产生3个明显的共振散射峰,其中496 nm处的散射峰强度最大且最稳定,适于进行共振光散射比浊法分析.探讨了共振光散射比浊法的机理,建立了在酸性氯化钠-乙二醇溶液中,共振光散射比浊法测定银离子的新方法.线性回归方程IRLS=7.60cAg+-4.82,R=0.999 8;检出限0.04μg/mL.此方法用于实际样品测定,取得了满意效果.

共振光散射光谱法测定痕量氯离子

Chloride ion was determined by the resonance light scattering method.The results showed the RLS intensity of the blank solution was faint.While chloride ion was added,the RLS intensity enhanced quickly and was proportional to the concentration of choloride ions.The assay is simple,rapid and sensitive with the linear range up to 14.0 μg/mL and the detection limit at 0.04 μg/mL.The recoveries of the method found to be in the range of 98.7%—102% with RSD between 1.1% and 2.2%.This assay was applied to the determination of chloride ions in acid copper plating bath and in tap water,and the results were satisfactory.

共振光散射比浊法测定有机磷农药残留量

A method of enhanced effect on calciumion and phosphate anion by resonance light-scattering turbidimetry(RLST) was studied in this paper.The optimum reaction conditions and the quantitative relationships of the intensities of RLST to the concentration of phosphate anion were established.The RLS intensity at 515nm is max and proportional to the concentration of phosphate anion.The method is simple and rapid with a detection limit of 0.00608μg/mL and a linear rang of 0.02～3.0μg/mL.It was applied to the determination of organophorus pesticide residues with satisfactory results.

共振光散射比浊法测定环境水样中Pb(Ⅱ)

基于铅与重铬酸钾反应产生沉淀拟定了一种测定铅的共振光散射比浊法.通过实验确定了适宜的反应条件.实 验表明,在λ=497 nm处,该体系有较大且较稳定的共振光散射强度,并且共振光散射强度与铅浓度成线性关系,线 性范围为0.1-1.0μg/ml,检测限为0.0573μg/mL.该方法快速,灵敏,简便.将该方法用于实际含铅水样中的铅的测定 并与原子吸收光谱法比较,结果较为满意.

共振光散射比浊法测定钾

The enhanced effect with Potassium and Na-TPB by Resonance Light Scattering Turbidimetry（RLST） based on Potassium and Na-TPB form hydrophobe sol system was studied in this paper.The optimum reaction conditions and the quantitative relationships of the intensities of RLS to the concentration of potassium were established.The RLS intensity at 578 nm is maximum and proportional to the concentration of potassium.The method is simple and rapid with a detection limit is 0.124×10-6mol/L and a linear range is 0.0～4.6×10-4mol/L.

血液中C反应蛋白的SPR传感检测方法研究

利用表面等离子体共振（SPR）生物传感技术，建立了一种可以快速准确分析血样中C反应蛋白的新方法。SPR传感器采用Kretschmann结构的角度连续扫描方式，使用2个步进电机分别驱动棱镜和光电检测器件转动进行单一样本的高精度分析。将C反应蛋白抗体修饰于敏感芯片表面，通过SPR传感器对血样中C反应蛋白浓度进行检测分析。利用SPR方法和传统免疫比浊法分别检测标准C反应蛋白样本和200份感染性疾病儿童患者（7～10岁，男112名，女88名）的血液样本，结果表明：SPR方法检测标准样本的线性变化区域更大。在患儿血样的检测中，尽管2种方法的结果基本相同，但是SPR检测速度更快，样本需求量更小、重复性更佳。这表明SPR生物传感分析方法在C反应蛋白检测中比传统方法更具优势，有望在临床检验分析中得以广泛应用。

共振光散射比浊法测定啤酒中氯离子的研究

本文采用共振光散射比浊法测定了啤酒中的氯离子含量,使用普通荧光分光光度计研究了形成氯化银胶体的各种实验条件.结果表明：该方法的检测波长607nm,线性范围0-14μg/mL,线性回归方程的相关系数为0.9992,检测限为0.04μg/mL.该法测定啤酒中氯离子的相对标准偏差在1.5％-2.8％之间,加标回收率为99％-104％,取得了较为满意的效果.

药用真菌戴氏虫草中透明质酸的定性定量分析

目的:建立定性、定量分析真菌来源透明质酸的方法。方法:采用十六烷基三甲基溴化铵(CTAB)比浊法定性检测真菌发酵液中透明质酸,采用改良Bitter-Muir(BM)法、共振瑞利散射(RRS)法定量分析真菌发酵液中透明质酸,并比较两者的优劣。结果:CTAB能够与真菌发酵液中透明质酸形成沉淀,导致溶液浑浊。改良BM法测定透明质酸在10～50μg.mL-1范围内具有良好的线性关系,平均加样回收率(n=9)为97.52%;RRS法测定透明质酸在0.5～2.5μg.mL-1范围内具有良好的线性关系,平均加样回收率(n=9)为100.1%。发酵基质中果糖、蔗糖、乳糖、葡萄糖等碳源可严重影响BM法的测定结果,而对RRS法测定干扰小。结论:CTAB法可以作为快速鉴别真菌发酵生产透明质酸的检测方法,RRS法可作为真菌发酵生产透明质酸过程中大量样品的快速定量分析方法。