The resting potential is the potential difference that exists between the inner and outer sides of the cell membrane when the cell is not stimulated.The resting membrane potential is a key regulator of phenomena such ...The resting potential is the potential difference that exists between the inner and outer sides of the cell membrane when the cell is not stimulated.The resting membrane potential is a key regulator of phenomena such as cell proliferation,morphogenesis,migration and differentiation.In this paper,we proposed a model of electrified cell clusters that considers the resting potential of cell clusters in the resting state.By measuring the potential difference between the inner and outer sides of biological tissues,it is verified that the cell cluster has a negative potential difference when taking the outer potential as the reference.In the absence of external conductors,the tissue is electrically neutral;while in the presence of external conductors,the positive net charges escape freely under repulsive forces and the biological tissues form a negative electrical equilibrium system.The model proposed in this study explored the potential situation above the cellular level in the resting state,providing a new perspective for the research on resting potential.展开更多
An injury potential is the direct current potential difference between the site of spinal cord injury and the healthy nerves. Its initial amplitude is a significant indicator of the severity of spinal cord injury, and...An injury potential is the direct current potential difference between the site of spinal cord injury and the healthy nerves. Its initial amplitude is a significant indicator of the severity of spinal cord injury, and many cations, such as sodium and calcium, account for the major portion of injury potentials. This injury potential, as wel as injury current, can be modulated by direct current field stimulation;however, the appropriate parameters of the electrical field are hard to define. In this paper, injury potential is used as a parameter to adjust the intensity of electrical stimulation. Injury potential could be modulated to slightly above 0 mV （as the anode-centered group） by placing the anodes at the site of the injured spinal cord and the cathodes at the rostral and caudal sections, or around-70 mV, which is resting membrane potential （as the cathode-centered group） by reversing the polarity of electrodes in the anode-centered group. In addition, rats receiving no electrical stimulation were used as the control group. Results showed that the absolute value of the injury potentials acquired after 30 minutes of electrical stimulation was higher than the control group rats and much lower than the initial absolute value, whether the anodes or the cathodes were placed at the site of injury. This phenomenon il ustrates that by changing the polarity of the electrical field, electrical stimulation can effectively modulate the injury potentials in rats after spinal cord injury. This is also beneficial for the spontaneous repair of the cel membrane and the reduction of cation influx.展开更多
基金This work was partially supported by the National Natural Science Foundation of China(No.62271023)the Beijing Natural Science Foundation(No.7202102)the Fundamental Research Funds for Central Universities.
文摘The resting potential is the potential difference that exists between the inner and outer sides of the cell membrane when the cell is not stimulated.The resting membrane potential is a key regulator of phenomena such as cell proliferation,morphogenesis,migration and differentiation.In this paper,we proposed a model of electrified cell clusters that considers the resting potential of cell clusters in the resting state.By measuring the potential difference between the inner and outer sides of biological tissues,it is verified that the cell cluster has a negative potential difference when taking the outer potential as the reference.In the absence of external conductors,the tissue is electrically neutral;while in the presence of external conductors,the positive net charges escape freely under repulsive forces and the biological tissues form a negative electrical equilibrium system.The model proposed in this study explored the potential situation above the cellular level in the resting state,providing a new perspective for the research on resting potential.
基金supported by the National Natural Science Foundation of China,No.51177162
文摘An injury potential is the direct current potential difference between the site of spinal cord injury and the healthy nerves. Its initial amplitude is a significant indicator of the severity of spinal cord injury, and many cations, such as sodium and calcium, account for the major portion of injury potentials. This injury potential, as wel as injury current, can be modulated by direct current field stimulation;however, the appropriate parameters of the electrical field are hard to define. In this paper, injury potential is used as a parameter to adjust the intensity of electrical stimulation. Injury potential could be modulated to slightly above 0 mV （as the anode-centered group） by placing the anodes at the site of the injured spinal cord and the cathodes at the rostral and caudal sections, or around-70 mV, which is resting membrane potential （as the cathode-centered group） by reversing the polarity of electrodes in the anode-centered group. In addition, rats receiving no electrical stimulation were used as the control group. Results showed that the absolute value of the injury potentials acquired after 30 minutes of electrical stimulation was higher than the control group rats and much lower than the initial absolute value, whether the anodes or the cathodes were placed at the site of injury. This phenomenon il ustrates that by changing the polarity of the electrical field, electrical stimulation can effectively modulate the injury potentials in rats after spinal cord injury. This is also beneficial for the spontaneous repair of the cel membrane and the reduction of cation influx.
