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Effect of Enhanced UV-B Radiation on Seed Setting Rate and 1 000-grain Weight of Indica Hybrid Rice Restorer Lines
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作者 况浩池 曾祥瑞 +5 位作者 罗俊涛 曾正明 杨扬 陈光珍 何兴材 付均 《Agricultural Science & Technology》 CAS 2013年第2期226-229,共4页
[Objective] This study was to investigate the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight of hybrid rice combinations. [Method] The seed setting rate and 1 000-grain weight of 10 new ... [Objective] This study was to investigate the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight of hybrid rice combinations. [Method] The seed setting rate and 1 000-grain weight of 10 new sterile indica restorer lines planted in pots under enhanced UV-B radiation and fluorescent lamps (control) were respectively measured, and the differences were compared. [Result] The enhanced UV-B radiation significantly reduced the seed setting rate of indica restorer lines, and the differences between that UV-B radiation treatment and control all reached extremely significant level. In addition, the enhanced UV-B radiation reduced the 1 000-grain weight of most indica restorer lines, and compared with that of control the difference achieved significant or very significant level. However, the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight differed to different indica restorer lines, and the differences among restorer lines tested were significant or very significant, which indicated the possibility to screen antiUV-B radiation rice materials and combinations. Finally, the indica restorer lines 09R-14, Luhui 37 and 10R-7703 which were strongly resistant to UV-B radiation were screened out. [Conclusion] This study laid foundation for breeding hybrid rice varieties resistance to UV-B radiation. 展开更多
关键词 UV-B radiation Indica restorer lines Hybrid rice Seed setting rate Grain weight
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Study on the combination ability and stability of rice restore line Yihui1577
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作者 包灵丰 林刚 +2 位作者 赵德明 曾健 李云武 《Agricultural Science & Technology》 CAS 2006年第2期2-8,共7页
Yihui1577 was bred after over 10 generations by crossing R16 and Yunan purple rice. Many new hybrid rice varieties have been bred with Yihui1577, of which Gangyou1577. Ilyou1577, Nyou1577 and Yixiang1577 were certifie... Yihui1577 was bred after over 10 generations by crossing R16 and Yunan purple rice. Many new hybrid rice varieties have been bred with Yihui1577, of which Gangyou1577. Ilyou1577, Nyou1577 and Yixiang1577 were certified by Sichuan Crop Variety Release Committee, Gangyou1577 and Yixiang1577 were certified by National Crop Variety Release Committee. The series varieties bred with Yihui1577 have been used widely with accumulated area of 1,200,000 ha. By using Yihui1577 as breeding material, an excellent restore line Yihui3003 and some other restore lines, such as Yihui1979 and Yihui315 with good quality and combination ability have been bred. Analysis of combination ability and stability of restore line Yihui1577 was made by using agricultural experimentation and statistical approach. The combination ability analysis was made by not complete dual crossing method with 4 CMS lines and 6 restorer lines. It shows that the general combination ability effect value of Yihui1577 is 0.226, while Minghui63 is -0.113. So the general combination ability of Yihui1577 is prior to that of Minghui63. Stability analysis shows that the F value of Gangyou1577 is 1.81378, while Sanyou63 is 1.40327, both doesn't reach significant level. So Gangyou1577 has the same stability as Sanyou63.The same conclusion is got in the stability analysis of the breeds of II you 1577, Yixiang1577, Nyou 1577 and so on. 展开更多
关键词 RICE restore line Yihui1577 combination ability STABILITY
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Identification of a Cytoplasmic Male Sterile Line NEA in Brassica napus L.and Its Genetic Studies 被引量:10
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作者 JIANG Liang-cai, PU Xiao-bin, ZHANG Qi-xing, CHEN Fang and ZHANG Jin-fang( The Crop Institute of the Sichuan Academy of Agricultural Sciences , Chengdu 610066 , P.R. China SichuanUniversity, Chengdu 610064 , P.R. China) 《Agricultural Sciences in China》 CAS CSCD 2002年第4期381-387,共7页
Male sterile NEA plants were identified in progenies of the radiated 92P x Aggregate-silique in Brassica napus L. in 1993. Their progeny plants from test crossing and open pollination were 100% male sterile. The doubl... Male sterile NEA plants were identified in progenies of the radiated 92P x Aggregate-silique in Brassica napus L. in 1993. Their progeny plants from test crossing and open pollination were 100% male sterile. The double-low male sterile lines JL-4 and JL-18 were bred through successive backcrossing of the double -low variety No.4 and No. 18 in Brassica napus L.to NEA. Restorer lines 6720 and 6722 with significant heterosis in F1 were developed. The results from investigating the restoring-ntaintaining relationship and inheritance of the restorer gene indicated that JL-4 and JL-18 were different from both PolCMS and Shan 2A type, and their restorer gene was controlled by a pair of dominant genie genes. 展开更多
关键词 Brassica napus L. Male sterile line Restorer line HEREDITY
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Breeding and Application of Kehui 36, a Coldtolerant Rice Restorer Line
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作者 邓小书 吴开其 +3 位作者 郑燊 韩龙植 刘艳 赵正武 《Agricultural Science & Technology》 CAS 2016年第1期75-77,共3页
Kehui 36, derived from Minghui 63 / Taiyin 1(♀) // Nuo 89-1(♂), is a new rice restorer line developed by Chongqing Normal University, which exhibits strong cold tolerance, high restoring ability, large pollen am... Kehui 36, derived from Minghui 63 / Taiyin 1(♀) // Nuo 89-1(♂), is a new rice restorer line developed by Chongqing Normal University, which exhibits strong cold tolerance, high restoring ability, large pollen amount and other characteristics. In 2013, Kehui 36 was technically identified by Chongqing Crop Variety Appraisal Committee. Gangyou 36, a hybrid line derived from Gang 46 A / Kehui 36,was registered and released for commercial production by Chongqing Crop Variety Appraisal Committee in 2012. 展开更多
关键词 Hybrid rice Restorer line Kehui 36 BREEDING
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Thermal Resistance of Common Rice Maintainer and Restorer Lines to High Temperature During Flowering and Early Grain Filling Stages 被引量:5
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作者 FU Guan-fu SONG Jian +5 位作者 XIONG Jie LIAO Xi-yuan ZHANG Xiu-fu WANG Xi LE Ming-kai TAO Long-xing 《Rice science》 SCIE 2012年第4期309-314,共6页
Fifteen common rice maintainer lines and 26 high-yielding restorer lines were used to evaluate their thermal resistance and fertility during flowering and early grain filling stages. The rice plants were subjected to ... Fifteen common rice maintainer lines and 26 high-yielding restorer lines were used to evaluate their thermal resistance and fertility during flowering and early grain filling stages. The rice plants were subjected to high temperature stress (39-43 ℃) for 1-15 d from main stem flowering. Based on the heat stress index, they were divided into thermal resistant lines, semi-thermal resistant lines, semi-thermal sensitive lines and thermal sensitive lines. Therefore, the maintainer lines K22B, Bobai B and V20B belonged to thermal resistant lines, whereas 11-32B, Zhongzhe B and Zhong 9B belonged to thermal sensitive lines. For rice restorer lines, Minghui 63 had the highest thermal resistance, followed by R207, P32, P929, and the lowest thermal resistant lines P62-2-2, R8006 and P51. The correlation analysis indicated that the heat stress index was significantly correlated with seed-setting rate and abortive grain rate under heat stress, but not under natural conditions. This indicated that heat stress occurred during flowering and early grain filling stages mainly decreased the seed- setting rate and significantly increased the abortive grain rate in both rice maintainer and restorer lines. 展开更多
关键词 RICE maintainer line restorer line heat stress FERTILITY thermal resistance
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Breeding and Characterization of a New Rice Restorer Line Containing Bt Gene 被引量:3
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作者 GAO Fang-yuan LU Xian-jun +7 位作者 HE Shu-lin CHEN Xiao-juan Lu Dai-hua SUN Shu-xia LI Zhi-hua LIU Guang-chun ZHANG Yi-zheng REN Guang-jun 《Rice science》 SCIE 2009年第3期194-200,共7页
Bt5198, a new rice restorer line containing Bt gene, was developed from the cross and backcross of the elite restorer line Chenghui 177 with Bt Minghui 63, a transgenic Bt restorer line. The inbred lines were evaluate... Bt5198, a new rice restorer line containing Bt gene, was developed from the cross and backcross of the elite restorer line Chenghui 177 with Bt Minghui 63, a transgenic Bt restorer line. The inbred lines were evaluated using PCR amplification, test paper evaluation, insect resistance evaluation in both the laboratory and paddy fields, nursery evaluation of rice blast resistance and pedigree selection of agronomic traits. Larval mortalities on Bt5198 and Bt Minghui 63 were 100% when rice culms were inoculated with the eggs of the striped stem borer (SSB) in the laboratory. Bt5198 was highly resistant against SSB and the yellow stem borer (YSB) under field conditions. The F1 hybrids derived from Bt5198 and four cytoplasmic male sterile (CMS) lines were also highly resistant to SSB and YSB and had a significant heterosis. Two-year evaluation of rice blast resistance confirmed that the resistance levels of Bt5198 to leaf blast and neck blast were similar to those of Chenghui 177 and significantly better than those of Bt Minghui 63. Seed germination ability and pollen yield of Bt5198 were similar with Chenghui 177, suggesting that the introduction of the Bt gene into the new restorer line had no significant effects on seed vitality or the yield of seed production. To identify the presence of the Bt gene, it was effective to combine test paper examination with the evaluation of insect-resistance, both in the laboratory and under field conditions. 展开更多
关键词 hybrid rice restorer line Bt gene insect resistance Magnaporthe oryzae seed germination pollen yield
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Development of elite restoring lines by integrating blast resistance and low amylose content using MAS 被引量:2
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作者 XIAO Wu-ming PENG Xin +10 位作者 LUO Li-xin LIANG Ke-qin WANG Jia-feng HUANG Ming LIU Yong-zhu GUO Tao LUO Wen-long YANG Qi-yun ZHU Xiao-yuan WANG Hui CHEN Zhi-qiang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第1期16-27,共12页
Blast resistance and grain quality are major problems in hybrid rice production in China. In this study, two resistance (R) genes, Pi46 and Pita, along with the gene Wxb, which mainly affects rice endosperm amylose ... Blast resistance and grain quality are major problems in hybrid rice production in China. In this study, two resistance (R) genes, Pi46 and Pita, along with the gene Wxb, which mainly affects rice endosperm amylose content (AC), were introgressed into an elite indica restoring line, R8166, which has little blast resistance and poor grain quality through marker-assisted selection (MAS). Eight improved lines were found to have recurrent genome recovery ratios ranging from 88.68 to 96.23%. Two improved lines, R163 and R167, were selected for subsequent studies. R167, which has the highest recovery ratio (96.23%), showed no significant differences in multiple agronomic traits. In contrast, R163 with the lowest recovery ratio (88.68%) exhibited significant differences in heading date and yield per plant compared with the recurrent parent. At two developmental stages, R163 and R167 had greatly enhanced resistance to blast over the recurrent parent. Similar trends were also observed for agronomic traits and blast resistance in R163- and R167-derived hybrids when compared with the counterparts from R8166. In addition, R163, R167, and their derived hybrids significantly improved the grain quality traits, including amylose content (AC), gel consistency (GC), chalky grain rate (CGR), and degree of endosperm chalkiness (DEC). It confirmed the success of efficiently developing elite restoring lines using MAS in this study. 展开更多
关键词 RICE restoring line blast resistance grain quality MAS
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Genetic Analysis of Stripe Disease Resistance in Rice Restorer Line C224 Using Major Gene plus Polygene Mixed Effect Model 被引量:6
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作者 ZHENG Wen-Jing LIU Zhi-Heng +1 位作者 ZHAO Jia-Ming CHEN Wen-Fu 《Rice science》 2012年第3期202-206,共5页
The inheritance of stripe disease resistance in a rice restorer line C224 was analyzed using the mixed effect model of major gene plus polygene for quantitative traits.In addition,the resistance was investigated in se... The inheritance of stripe disease resistance in a rice restorer line C224 was analyzed using the mixed effect model of major gene plus polygene for quantitative traits.In addition,the resistance was investigated in seven crosses of C224 with maintainer lines.The results showed that the stripe resistance of C224 was controlled by two major genes with additive-dominance-epistasis effects plus polygenes with additive-dominance effects (E-1 model).These two genes had additive effects of-12.47% and-24.75%,respectively,showing negative dominance effects.There were significant epistasis and interaction effects between the two major genes.The heritability of the two major genes was 92.12%,while that of polygenes was 2.74%,indicating that the stripe resistance had dominant major gene effect.Of the seven crosses,five displayed high or medium resistance to the stripe disease. 展开更多
关键词 RICE rice stripe resistance major gene plus polygene mixed genetic model restorer line
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Indica rice restorer lines with large sink potential exhibit improved nutrient transportation to the panicle,which enhances both yield and nitrogen-use efficiency 被引量:2
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作者 TIAN Qing-lan HE Lian-hua +5 位作者 LIAO Shuang LI Wu DENG Fei ZHOU Wei ZHONG Xiao-yuan REN Wan-jun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第6期1438-1456,共19页
The yield and nitrogen use efficiency(NUE)of hybrid rice combinations are closely related to restorer line.Therefore,it is essential to evaluate the agronomic characteristics of restorer lines with high yield and high... The yield and nitrogen use efficiency(NUE)of hybrid rice combinations are closely related to restorer line.Therefore,it is essential to evaluate the agronomic characteristics of restorer lines with high yield and high NUE(HYHN).However,it is unclear which restorer lines are HYHN,and neither have the common agronomic traits of the HYHN restorer lines been identified.Aiming to address this issue,we conducted two filed experiments using three nitrogen applications,which screened five HYHN restorer lines from 15 indica restorer lines.Yield,NUE and nutrient transportation of restorer lines with different yields and NUE types were examined.Yield and total nitrogen absorption in aboveground biomass(TNA)increased,whereas NUE for grain production decreased with increasing nitrogen application levels.The HYHN restorer lines had large spikelets and high weight per panicle that were significantly positively correlated with yield and NUE.Therefore,large sink potential may be beneficial for both yield and NUE.We further studied the differences in nutrient transportation to panicles between the HYHN and low yield and low NUE(LYLN)restorer lines and found that the former had a higher nitrogen absorption level and dry matter weight ratios of panicle in maturity.Moreover,the HYHN lines also had a higher root and neck-panicle node bleeding intensity per stem after heading and more developed vascular bundles of neck-panicle nodes and leaves than the LYLN lines,which could contribute to the transportation of nutrients from root to ground and from stem and leaf to spike.Therefore,the advantages of large sink potential of the HYHN restorer lines include large nutrient accumulation in and distribution to the panicles and smooth flow of nutrients along the transportation channels. 展开更多
关键词 indica hybrid rice restorer lines grain yield nitrogen-use efficiency sink potential nutrient transportation
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Resistance Evaluation of Some Chinese Leading Rice Maintainer, Restorer lines and Their Hybrids to Magnaporthe grisea 被引量:2
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作者 ZHUXu-dong SHENYing +2 位作者 HenriADREIT JulienFROUIN DidierHARREAU 《Rice science》 SCIE 2004年第3期101-105,共5页
Six isolates of Magnaporthe grisea were selected to inoculate on 10 Chinese leading maintainer lines (B-lines), 14 restorer lines (R-lines) and their F1 hybrid plants. In the tested rice materials, R-lines were proved... Six isolates of Magnaporthe grisea were selected to inoculate on 10 Chinese leading maintainer lines (B-lines), 14 restorer lines (R-lines) and their F1 hybrid plants. In the tested rice materials, R-lines were proved to be more resistant to blast than B-lines. The resistance frequency of about 25% F1 hybrid plants was less than their parents. In addition, 26 isolates of M. grisea collected from different rice growing areas of China were inoculated on 13 new improved hybrid rice combinations. The resistance frequencies of 5 improved hybrids were better than those of the controls and leading varieties in rice production of China. 展开更多
关键词 hybrid rice maintainer line restorer line Magnaporthe grisea BLAST RESISTANCE
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Breeding Rice Restorer Lines with High Resistance to Bacterial Blight by Using Molecular Marker-Assisted Selection 被引量:1
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作者 DENG Qi-ming WANG Shi-quan +2 位作者 ZHENG Ai-ping ZHANG Hong-yu LI Ping 《Rice science》 SCIE 2006年第1期22-28,共7页
Two bacterial blight (BB) resistance genes, Xa21 and Xa4, from IRBB24 were introduced into hybrid rice restorer line Mianhui 725, which is highly susceptible to BB, by using hybridization and molecular marker-assist... Two bacterial blight (BB) resistance genes, Xa21 and Xa4, from IRBB24 were introduced into hybrid rice restorer line Mianhui 725, which is highly susceptible to BB, by using hybridization and molecular marker-assisted selection technology. Four homologous restorer lines were obtained through testing the R target genes with molecular markers and analyzing parental genetic background. Inoculation of the four lines and their hybrids with the specific strains of Xanthomonas oryzae pv. oryzae, P1, P6 and seven representative strains of Chinese pathotype, C Ⅰ -CⅦ, showed that all of the four lines and their hybrids were highly resistant and presented broad resistance-spectrum to BB. The hybrids of G46A / R207-2 displayed good agronomic characters and high yield potential, and R207-2 was named Shuhui 207. 展开更多
关键词 bacterial blight RESISTANCE gene pyramiding hybrid rice restorer line molecular marker-assisted selection BREEDING
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Breeding of R8012,a Rice Restorer Line Resistant to Blast and Bacterial Blight Through Marker-Assisted Selection
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作者 ZHAN Xiao-deng ZHOU Hai-peng +3 位作者 CHAI Rong-yao ZHUANG Jie-yun CHENG Shi-hua CAO Li-yong 《Rice science》 SCIE 2012年第1期29-35,共7页
Genetic improvement is one of the most effective strategies to prevent rice from blast and bacterial blight (BB) diseases, the two most prevalent diseases jeopardizing rice production. Rice hybrids with dural resist... Genetic improvement is one of the most effective strategies to prevent rice from blast and bacterial blight (BB) diseases, the two most prevalent diseases jeopardizing rice production. Rice hybrids with dural resistance to blast and BB are needed for sustainable production of food. An incomplete diallele design resulted in 25 crosses between five blast and five BB resistant germplasm accessions. Only one pair of parents, DH146 ×TM487, showed polymorphism for all the markers to identify one blast resistance gene Pi25 and three BB resistance genes, Xa21, xa13 and xa5, thus it was used in the marker-assisted selection (MAS). F2 individuals of DH146× TM487 were genotyped using flanking markers of RM3330 and sequence tagged site (STS) marker SA7 for Pi25. The resistant F2 plants with Pi25 were used for pyramiding BB resistance genes Xa21, xa13 and xa5 identified by the markers pTA248, RM264 and RM153, respectively in subsequent generations. Finally, after selection for agronomic traits and restoration ability among 12 pyramided lines, we acquired an elite restorer line, R8012 including all four target genes (Pi25+Xa21+xa13+xa5). Hybrid Zhong 9NR8012 derived from the selected line showed stronger resistance to blast and BB, and higher grain yield than the commercial checks uniformally in experimental plots, 2007 state-wide yield trial and 2008 nation-wide yield trial. This study provides a paradigmatic example to show that MAS is a practically feasible tool in effectively pyramiding multiple resistance genes. The resultant restoring line and its hybrid would play an important role in securing rice production in China. 展开更多
关键词 rice restorer line bacterial blight BLAST marker-assisted selection gene pyramiding BREEDING
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Analysis on the combination ability and stability of rice restore line Yihui1577
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作者 BAO Lingfeng LIN Gang ZHAO Deming LI Yunwu HE Bing 《Journal of Northeast Agricultural University(English Edition)》 CAS 2007年第4期301-305,共5页
Yihui1577 was bred after over 10 generations by crossing RI6 and Yunnan purple rice. Many new hybrid rice varieties had been bred with Yihui 1577, of which Gangyou 1577, Ⅱ you1577, Nyou 1577 and Yixiang1577 were cert... Yihui1577 was bred after over 10 generations by crossing RI6 and Yunnan purple rice. Many new hybrid rice varieties had been bred with Yihui 1577, of which Gangyou 1577, Ⅱ you1577, Nyou 1577 and Yixiang1577 were certified by Sichuan Province Crop Variety Release Committee, Gangyou1577 and Yixiang1577 were certified by National Crop Variety Release Committee. The series varieties bred with Yihui1577 had been used widely with accumulated areas of 1 200 000 hm2. By using Yihui1577 as breeding material, an excellent restore line Yihui 3003 and some other restore lines, such as Yihui 1979, Yihui 315 with good rice quality and good combination ability had been bred. Analysis of combination ability and stability about restore line Yihui 1577 was made by using agricultural experimentation and statistical approach. The combination ability analysis was made in complete dual crossing method with 4 CMS lines and 6 restorer lines. It showed that the general combination ability effect value of Yihui1577 was 0.226, while Minghui63 was -0.113. So the general combination ability ofYihui1577 was prior to that of Minghui 63. Stability analysis showed that the F value of Gangyou1577 was 1.81378, while Sanyou 63 was 1.40327, both didn't reach significant level. So Gangyou1577 had the same stability as Sanyou 63. The same conclusion had got in the stability analysis of the breeds of Ⅱ you 1577, Yixiang 1577, Nyou 1577 and so on. 展开更多
关键词 RICE restore line Yihui 1577 combination ability STABILITY
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Combining Ability of Yield Traits of Rice Restorer Lines in China and South Asia
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作者 游书梅 曹应江 +10 位作者 郑家奎 蒋开锋 张涛 杨莉 杨乾华 万先齐 郭小蛟 秦俭 罗婧 李昭祥 高磊 《Agricultural Science & Technology》 CAS 2017年第3期478-482,共5页
[Objective] This study was conducted to analyze the difference in the general combining ability of yield traits of rice varieties in China and South Asia and to provide reference for the introduction and utilization o... [Objective] This study was conducted to analyze the difference in the general combining ability of yield traits of rice varieties in China and South Asia and to provide reference for the introduction and utilization of rice restorer lines from South Asia. [Method] A total of 111 hybrids were obtained by crossing 37 restorer lines in China and South Asia with Gang 46A, Lu 98A and Dexiang 074 following a NC II design. The combining ability of eight yield traits of the hybrids was analyzed in Luzhou and Deyang bases of Sichuan Province. [Result] In both bases, the eight yield traits had significant or extremely significant differences between the combina- tions, restorer lines, sterile lines or sterile line^restorer line, suggesting that there were d^erences in the genetic effects between the combinations or lines. The gen- eral combining ability (GCA) variances were all higher than special combining ability (SCA) variances in parents, which indicated that these traits were affected by gene additive effect. Restorer lines contributed more to F1 generation than male sterile liens in the eight yield traits. Nine restorer lines from India and Bangladesh were ranked in the top 15 lines with high GCA in the number of productive tillers in both bases. [Co.cl.siee] There was significant differences in the combining ability in the eight yield traits between the restorer lines from China and South Asia. The lines from India and Bangladesh were better than those from China in the GCA of the number of productive tillers, and the lines from China were better than those from India and Bangladesh in the GCA of other traits. 展开更多
关键词 RICE Restorer line YIELD South Asia Combining ability
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Breeding of Rice Restorer Line Haihui 818 of Multi-resistant Gene Pyramiding
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作者 Xiaowei YAN Qingjie TANG +3 位作者 Yisheng HAN Huijian WANG Jing XU Funeng XING 《Agricultural Biotechnology》 CAS 2020年第6期8-10,共3页
Haihui 818 is a new restorer line by cross breeding with Hua 23 S and Huahui 8131.Good plant type,high tillering ability,fine grain quality,strong restoring ability and high yield were shown in hybrid production.After... Haihui 818 is a new restorer line by cross breeding with Hua 23 S and Huahui 8131.Good plant type,high tillering ability,fine grain quality,strong restoring ability and high yield were shown in hybrid production.After artificial inoculation and field stress and molecular market-assisted selection,the results showed that rice-blast resistance genes Pi1,BB resistance genes Xa7,and brown planthopper resistance genes Bph3,Bph14 and Bph15 were pyramided together.Among its hybrids,Bo II You 818(Bo II A/Haihui 818)was approved for commercial production by Hainan Provincial Crop Variety Approval Committee in 2015. 展开更多
关键词 Restorer line Haihui 818 Gene pyramiding Molecular market-assisted selection BREEDING
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Breeding dominant genic male sterility restorer line of Brassica napus L.
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作者 Junying Zhang Jianxia Jiang +6 位作者 Liyong Yang Meiyan Jiang Yanli Li Weirong Wang Chaocai Sun Jifeng Zhu Xirong Zhou 《Oil Crop Science》 2020年第2期90-95,共6页
To facilate breeding process of Brassica napus,a microspore culture and molecular marker-assisted screening combined system were proposed in this research.At early flowering stage,F1 offspring of hybridized combinatio... To facilate breeding process of Brassica napus,a microspore culture and molecular marker-assisted screening combined system were proposed in this research.At early flowering stage,F1 offspring of hybridized combination HY15 A×HF06 was used as donor for microspore culture to analyze effects of colchicine concentration on embryogenic and diploid rates of microspore.Treatment with 50 mg/L colchicine resulted in embryogenic rate of3.56 embryos/bud,which was substantially higher than control(0.78 embryos/bud).A total of 1,387 embryos and 862 single plants were obtained after induction culture.Ploidy detection was performed for the regenerated plants by flow cytometry.Diploid rates of microspores treated with 50 mg/L and 70 mg/L colchicine were 17.2%and 21.0%respectively,which was significantly higher than control(10.5%).Totally 108 single plants that doubled successfully were randomly selected and screened using molecular marker BE10.Approximately 54 of108 plants generated a 305 bp amplification product,whereas the other 54 plants showed a 398 bp band,thereby satisfying 1:1 separation ratio(x0.052=0.0093).These coincided with field identification results.Findings of this study indicated that homozygous breeding material could be obtained by microspore culture in a short time,thereby remarkably accelerate breeding. 展开更多
关键词 Brassica napus L. MICROSPORES Double haploid Restorer line COLCHICINE
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Selection of wide compatible restorer lines and their application in hybrid rice breeding
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作者 XIE Xiaobo, ZHANG Shanqing, and FANG Hongming, CNRRI, Hangzhou 310006, China 《Chinese Rice Research Newsletter》 1997年第3期10-11,共2页
Since the middle of 1980’s, wide compatibility(WC) rice lines have been screened by ricebreeders in China and applied in hybrid ricebreeding program. Several WC lines such asPecos, T984, Lunhui 422, and 02428 withide... Since the middle of 1980’s, wide compatibility(WC) rice lines have been screened by ricebreeders in China and applied in hybrid ricebreeding program. Several WC lines such asPecos, T984, Lunhui 422, and 02428 withideal agronomic characters were identified. Weincorporated the WC gene into restorer linesby crossing these japonica WC lines with ob-tained indica lines. Some WC restorer lineswith indica-japonica medium type were ob-tained and their application value in intersub-specific hybrid rice breeding were evaluated. 1. Effect of crossing methods on selectionefficiencies of WC restorer lines 展开更多
关键词 line WC Selection of wide compatible restorer lines and their application in hybrid rice breeding
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Inducing fertility restoration in the genic male sterile line of rice with chemical regulators
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作者 WANG Kaizhi,CNRRI 《Chinese Rice Research Newsletter》 1994年第3期11-11,共1页
For the first time, the fertility of rice genic male sterile line was partially restored with the application of chemical regulators at Hainan Rice Breeding Nursery on Mar 1993. A single panicle of the rice plant coul... For the first time, the fertility of rice genic male sterile line was partially restored with the application of chemical regulators at Hainan Rice Breeding Nursery on Mar 1993. A single panicle of the rice plant could bear as many as 27 grains. The chemical agent that could modulate the growth of the rice plants of genic male sterile line was developed by Prof ZHOU Guangqia and his colleagues at the Biology Institute, Hunan Teachers University, China. 展开更多
关键词 line Inducing fertility restoration in the genic male sterile line of rice with chemical regulators
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Construction of SSR Linkage Map and Analysis of QTLs for Rolled Leaf in Japonica Rice 被引量:9
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作者 GUO Yuan CHENG Bao-shan HONG De-lin 《Rice science》 SCIE 2010年第1期28-34,共7页
Genetic analysis of rolled leaf is important to rice ideotype breeding. To detect loci controlling rolled leaf of japonica restorer lines, SSR marker genotypes and phenotypes of flag leaf rolling index (LRI) were in... Genetic analysis of rolled leaf is important to rice ideotype breeding. To detect loci controlling rolled leaf of japonica restorer lines, SSR marker genotypes and phenotypes of flag leaf rolling index (LRI) were investigated in Xiushui 79 (P1, a japonica rice variety), C Bao (P2, a japonica restorer line) and 254 recombinant inbred lines derived from the cross between P1 and P2 , and in two environments. A genetic map of this cross was constructed, QTLs for LRI were detected and their interactions with environments were analyzed. Among 818 pairs of SSR primers, 90 primers showed polymorphism between P1 and P2, and 12 markers showed highly significant correlation with LRI in both environments based on single marker regression analysis. The genetic map containing 74 information loci has a total distance of 744.6 cM, with an average of 10.1 cM between two adjacent loci. Three QTLs (qRL-1, qRL-7 and qRL-8-1) were detected with two softwares: WinQTLCart 2.5 and QTLNetwork2.0. qRL-8-1 was a new locus, accounting for 15.5% and 12.8% of phenotypic variations in the two environments, respectively. The phenotypic variation explained by additive effect was 6.6%. No interaction was found between qRL-8-1 genotype and environments. 展开更多
关键词 japonica rice restorer line leaf rolling index quantitative trait locus genotype and environment interaction
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