[Objective] This study was to investigate the restoring ability of normal indica red rice Ruby and to carry out its restoring gene mapping. [Method] Normal indica red rice Ruby was hybridized with the sterile lines Zh...[Objective] This study was to investigate the restoring ability of normal indica red rice Ruby and to carry out its restoring gene mapping. [Method] Normal indica red rice Ruby was hybridized with the sterile lines Zhenxian 97A, D62A, G46A and D702A to prepare their F1, BC1 and F2 progenies, and the pollen fertilities of these progenies were investigated. Meanwhile the restoring genes were mapped using SSLP. [ Result] For the sterile lines tested, Ruby has a gene to restore their fertilities. This gene is located on the chromosome 7 and shows a genetic distance of 7.4 cM with RM182. Unlike the clustering distribution of the restoring genes on chromosome 10, it is a specific restoring gene. [ Conclusion] it is feasible to breed restoring genes controlling red color characters via transgene and backcross.展开更多
基金Supported by Sci-tech Program for Excellent Young Scientists of Sichuan Province(01ZQ052)~~
文摘[Objective] This study was to investigate the restoring ability of normal indica red rice Ruby and to carry out its restoring gene mapping. [Method] Normal indica red rice Ruby was hybridized with the sterile lines Zhenxian 97A, D62A, G46A and D702A to prepare their F1, BC1 and F2 progenies, and the pollen fertilities of these progenies were investigated. Meanwhile the restoring genes were mapped using SSLP. [ Result] For the sterile lines tested, Ruby has a gene to restore their fertilities. This gene is located on the chromosome 7 and shows a genetic distance of 7.4 cM with RM182. Unlike the clustering distribution of the restoring genes on chromosome 10, it is a specific restoring gene. [ Conclusion] it is feasible to breed restoring genes controlling red color characters via transgene and backcross.