Background There are no data comparing a regular diet with a restricted diet after endoscopic polypectomy in patients with colorectal polyps.The current guidelines also did not provide the detailed information of diet...Background There are no data comparing a regular diet with a restricted diet after endoscopic polypectomy in patients with colorectal polyps.The current guidelines also did not provide the detailed information of dietary patterns after polypectomy.In this study,we aimed to evaluate the safety and efficacy of different diets on post-polypectomy outcomes.Methods A total of 302 patients with colorectal polyps who underwent polypectomy were prospectively enrolled between March 2019 and December 2019 in Nanfang Hospital(Guangzhou,China).Enrolled patients were then randomly assigned to a regular diet group or a restricted diet group after polypectomy.The study is a non-inferior design and the primary end point was the post-operative adverse events(AE)rate.Secondary end points included length of stay(LOS)and hospitalization cost.Results Among all the included patients,148 patients received a restricted diet and 154 patients received a regular diet after polypectomy.A total of 376 polyps were removed,with 183 polyps in the restricted diet group and 193 polyps in the regular diet group.Shorter LOS(4.0±1.4 vs 4.8±1.7,P<0.001)and lower hospitalization costs(7,701.63±2,579.07 vs 8,656.05±3,138.53,P=0.001)were observed in the regular diet group.In particular,there was no significant difference in 3-day AE rates between the restricted diet and the regular diet group(1.35%[2/148]vs 2.60%[4/154],P=0.685).Subgroup analysis looking at the number of polyps removed in each patient and different treatmentmodalities also showed similar findings.Conclusion Regular diet should be recommended after polypectomy for polyps<20mm as it can shorten LOS and save hospitalization costs.展开更多
Dietary restriction(DR) can delay senescence, prolong lifespan of mammals and improve their learning-memory activity. The purpose of the study was to explore the effects of DR on hypolipidemic action and liver funct...Dietary restriction(DR) can delay senescence, prolong lifespan of mammals and improve their learning-memory activity. The purpose of the study was to explore the effects of DR on hypolipidemic action and liver function of mice with hyperlipidemia. To investigate these effects, hyperlipidemia mouse models were established with high-fat diet(HFD)(34% of energy), then randomly divided into HFD group, DR30% group and DR50% group. Mice in DR30% and DR50% group were respectively supplied with HFD as much as about 70% and 50% of the consumption of HFD in the mice of HFD group. Rats in control group were fed routinely. After DR for 5 weeks, the average body weight, liver weight, liver index, serum lipids and glucose levels in both DR groups decreased significantly as compared with the HFD group(P〈0.05 or P〈0.01), so did alanine aminotransferase(ALT), aspartate aminotransferase(AST), lactate dehydrogenase(LDH) levels and the ratio of LDL-C/HDL-C in the DR50% group(P〈0.05 or P〈0.01). Histopathology examination of liver tissues further proved ameliorative effect of DR on liver function. Western blotting showed that DR significantly increased the expression of silent mating type information regulation 2 homolog 1(SIRT1) in liver and adipose, while notably decreased the expression of peroxisome proliferators-activated receptors-gamma(PPARγ) in adipose(P〈0.05 or P〈0.01). The increase of SIRT1 and decrease of PPARγ may be a mechanism by which DR reduces blood lipids and ameliorates liver function.展开更多
Numerous experimental life-history studies on aging are mainly baised on two classical models—fruit fly Drosophila melanogaster(Meigen)and nematode Caenorhab-ditis elegans(Maupas)—with relatively little attention gi...Numerous experimental life-history studies on aging are mainly baised on two classical models—fruit fly Drosophila melanogaster(Meigen)and nematode Caenorhab-ditis elegans(Maupas)—with relatively little attention given to other organisms with different life-history characters.Two-spotted spider mite Tetranychus urticae Koch(Acari:Tetranychidae)differs from many other arthropods in that the females continue their growth in the early adult stage and can reproduce sexually and asexually.In this study,the influences of dietary restriction and delayed mating on the aging patterns of the spider mite were examined with the prevailing survival and reproduction trade-off hypothesis of aging being tested.Significant sex-specific responses of the spider mites were found.The females showed longevity extension on diet restriction(fasting for 2 days in every 4 days)compared with their counterparts being fed ad libitum,and after delayed mating for 9 days,while the males displayed a decrease in lifespan when experiencing diet restriction but were not significantly influenced by delayed mating.Path analysis was used to investigate the relationship between mite survival and reproduction traits,including longevity,female lifetime reproduction,age at first reproduction,early reproductive efforts and late reproductive efforts,yielding no evidence for trade-offs between these life-history traits.The additive effects of dietary restriction and delayed mating in lifespan extension of female spider mites were confirmed,proving that diet restriction is a robust anti-aging intervention,and that later onset of reproduction can prolong adult lifespan in females.展开更多
Purpose We sought to describe and examine the interrelationships between energy intake,body composition,and estimated energy balance.Methods Using self-reported hourly food intake and formula-based energy expenditure(...Purpose We sought to describe and examine the interrelationships between energy intake,body composition,and estimated energy balance.Methods Using self-reported hourly food intake and formula-based energy expenditure(EE)protocols,19 female profes-sional cheerleaders(mean age 25.4 years)were assessed to obtain energy balance(EB)for a typical training day.Energy intake(EI)was predicted using the USDA Food Composition Database SR27,and EE was predicted using the Harris-Benedict equation plus a MET-based relative intensity activity scale.Body composition was predicted using a multi-current,8-mode segmental bioelectrical impedance analysis system.Hourly and daily EB was calculated from EI and EE data.Results Subjects reported a 24 h EI significantly below(P<0.001)the unadjusted predicted energy requirement(1482 kcal vs.2199 kcal,respectively),resulting in an average negative net EB of−720 kcal.Carbohydrate intake was significantly below the minimum recommended level(3.1 g/kg vs.6 g/kg,P<0.001)while protein and fat intakes met the recommended levels.Higher fat intake(g/kg)was significantly associated with a higher EI kcal/kg(r=0.726;P<0.001),which was sig-nificantly associated(r=−0.55;P=0.01)with a lower body fat percent(BF%).Using the median of BF%(20.9)as the cut point,participants with fewer hours in a negative EB had lower BF%(P=0.043)and those with lower BF%spent more time in an EB of±300 kcal(P=0.013).Conclusions These athletes reported low energy intakes that resulted in large EB deficits and/or more hours in a negative EB,which could be counterproductive for achieving a lean body composition overtime.展开更多
Objective:To explore the levels of fibroblast growth factor 23(FGF23)during pregnancy and its relationship with intrauterine growth restriction(IUGR).Methods:Pregnant rats were classified into an ad libitum rat chow g...Objective:To explore the levels of fibroblast growth factor 23(FGF23)during pregnancy and its relationship with intrauterine growth restriction(IUGR).Methods:Pregnant rats were classified into an ad libitum rat chow group(ad libitum rat chow,AD group,n=25)and an undernutrition group(50%of their daily food requirement,UN group,n=25).The levels of maternal serum FGF23,tissue homogenate FGF23,and bone gla protein in fetal rats,and placental FGF23 mRNA and protein expression were examined by enzyme-linked immunosorbent assay,real-time qPCR analysis respectively.Finally,the effect of recombinant FGF23 on the viability of MG-63 cells was determined by cell proliferation assay.Data were analyzed with independent two-tailed t test and one-way analysis of variance.Spearman rank-order correlation coefficients(continuous variables)was performed to determine the relationship of results.Results:The diet restriction induced IUGR in rat offsprings,and the UN group exhibited a significantly lower FGF23 level(P<0.05,n=5).The FGF23 level was increased and peaked in maternal serum on gestation day(GD)15,but peaked in fetal and placenta on GD20.Moreover,the tissue homogenate levels of FGF23 and bone gla protein in fetal rats in both groups were positively correlated(r=0.923,P<0.05;r=0.925,P<0.05,respectively,n=15),FGF23 was localized to both decidual and labyrinth zones,with remarkably higher expression on GD20,P<0.05,n=5.In vitro,recombinant human FGF23 enhanced MG-63 cell viability,P<0.05,n=5.Conclusion:Prenatal undernutrition could decrease the FGF23 expression in fetal rats caused by the mother through the placenta,and induced the IUGR and hindered the ossification.And the FGF23 levels are peaked on GD15 mother but peaked on GD20 placenta and fetuses,these might be associated with the over compensation of maternal placenta on GD20.展开更多
基金supported by the Science and Technology Planning Project of Guangdong Province[grant number 2017A020215139]Guangdong Gastrointestinal Disease Research Center[grant number 2017B020209003].
文摘Background There are no data comparing a regular diet with a restricted diet after endoscopic polypectomy in patients with colorectal polyps.The current guidelines also did not provide the detailed information of dietary patterns after polypectomy.In this study,we aimed to evaluate the safety and efficacy of different diets on post-polypectomy outcomes.Methods A total of 302 patients with colorectal polyps who underwent polypectomy were prospectively enrolled between March 2019 and December 2019 in Nanfang Hospital(Guangzhou,China).Enrolled patients were then randomly assigned to a regular diet group or a restricted diet group after polypectomy.The study is a non-inferior design and the primary end point was the post-operative adverse events(AE)rate.Secondary end points included length of stay(LOS)and hospitalization cost.Results Among all the included patients,148 patients received a restricted diet and 154 patients received a regular diet after polypectomy.A total of 376 polyps were removed,with 183 polyps in the restricted diet group and 193 polyps in the regular diet group.Shorter LOS(4.0±1.4 vs 4.8±1.7,P<0.001)and lower hospitalization costs(7,701.63±2,579.07 vs 8,656.05±3,138.53,P=0.001)were observed in the regular diet group.In particular,there was no significant difference in 3-day AE rates between the restricted diet and the regular diet group(1.35%[2/148]vs 2.60%[4/154],P=0.685).Subgroup analysis looking at the number of polyps removed in each patient and different treatmentmodalities also showed similar findings.Conclusion Regular diet should be recommended after polypectomy for polyps<20mm as it can shorten LOS and save hospitalization costs.
基金supported by a grant from the Social Development Research Program of Science and Technology Agency of Jiangsu Province(No.BE2015646)
文摘Dietary restriction(DR) can delay senescence, prolong lifespan of mammals and improve their learning-memory activity. The purpose of the study was to explore the effects of DR on hypolipidemic action and liver function of mice with hyperlipidemia. To investigate these effects, hyperlipidemia mouse models were established with high-fat diet(HFD)(34% of energy), then randomly divided into HFD group, DR30% group and DR50% group. Mice in DR30% and DR50% group were respectively supplied with HFD as much as about 70% and 50% of the consumption of HFD in the mice of HFD group. Rats in control group were fed routinely. After DR for 5 weeks, the average body weight, liver weight, liver index, serum lipids and glucose levels in both DR groups decreased significantly as compared with the HFD group(P〈0.05 or P〈0.01), so did alanine aminotransferase(ALT), aspartate aminotransferase(AST), lactate dehydrogenase(LDH) levels and the ratio of LDL-C/HDL-C in the DR50% group(P〈0.05 or P〈0.01). Histopathology examination of liver tissues further proved ameliorative effect of DR on liver function. Western blotting showed that DR significantly increased the expression of silent mating type information regulation 2 homolog 1(SIRT1) in liver and adipose, while notably decreased the expression of peroxisome proliferators-activated receptors-gamma(PPARγ) in adipose(P〈0.05 or P〈0.01). The increase of SIRT1 and decrease of PPARγ may be a mechanism by which DR reduces blood lipids and ameliorates liver function.
基金This study is financially supported by the PhD scholarship from China Scholarship Council and New Zealand Government core funding for Crown Research Institutes from the Ministry of Business,Innovation and Employmenfs Science and Innovation GroupDuring the preparation of this manuscript,Guang-Yun Li was founded by Fundamental Research Funds for the Central Universities SWU120048.
文摘Numerous experimental life-history studies on aging are mainly baised on two classical models—fruit fly Drosophila melanogaster(Meigen)and nematode Caenorhab-ditis elegans(Maupas)—with relatively little attention given to other organisms with different life-history characters.Two-spotted spider mite Tetranychus urticae Koch(Acari:Tetranychidae)differs from many other arthropods in that the females continue their growth in the early adult stage and can reproduce sexually and asexually.In this study,the influences of dietary restriction and delayed mating on the aging patterns of the spider mite were examined with the prevailing survival and reproduction trade-off hypothesis of aging being tested.Significant sex-specific responses of the spider mites were found.The females showed longevity extension on diet restriction(fasting for 2 days in every 4 days)compared with their counterparts being fed ad libitum,and after delayed mating for 9 days,while the males displayed a decrease in lifespan when experiencing diet restriction but were not significantly influenced by delayed mating.Path analysis was used to investigate the relationship between mite survival and reproduction traits,including longevity,female lifetime reproduction,age at first reproduction,early reproductive efforts and late reproductive efforts,yielding no evidence for trade-offs between these life-history traits.The additive effects of dietary restriction and delayed mating in lifespan extension of female spider mites were confirmed,proving that diet restriction is a robust anti-aging intervention,and that later onset of reproduction can prolong adult lifespan in females.
文摘Purpose We sought to describe and examine the interrelationships between energy intake,body composition,and estimated energy balance.Methods Using self-reported hourly food intake and formula-based energy expenditure(EE)protocols,19 female profes-sional cheerleaders(mean age 25.4 years)were assessed to obtain energy balance(EB)for a typical training day.Energy intake(EI)was predicted using the USDA Food Composition Database SR27,and EE was predicted using the Harris-Benedict equation plus a MET-based relative intensity activity scale.Body composition was predicted using a multi-current,8-mode segmental bioelectrical impedance analysis system.Hourly and daily EB was calculated from EI and EE data.Results Subjects reported a 24 h EI significantly below(P<0.001)the unadjusted predicted energy requirement(1482 kcal vs.2199 kcal,respectively),resulting in an average negative net EB of−720 kcal.Carbohydrate intake was significantly below the minimum recommended level(3.1 g/kg vs.6 g/kg,P<0.001)while protein and fat intakes met the recommended levels.Higher fat intake(g/kg)was significantly associated with a higher EI kcal/kg(r=0.726;P<0.001),which was sig-nificantly associated(r=−0.55;P=0.01)with a lower body fat percent(BF%).Using the median of BF%(20.9)as the cut point,participants with fewer hours in a negative EB had lower BF%(P=0.043)and those with lower BF%spent more time in an EB of±300 kcal(P=0.013).Conclusions These athletes reported low energy intakes that resulted in large EB deficits and/or more hours in a negative EB,which could be counterproductive for achieving a lean body composition overtime.
基金This work was supported by the National Natural Science Foundation of China(No.81571465,No.81871175)
文摘Objective:To explore the levels of fibroblast growth factor 23(FGF23)during pregnancy and its relationship with intrauterine growth restriction(IUGR).Methods:Pregnant rats were classified into an ad libitum rat chow group(ad libitum rat chow,AD group,n=25)and an undernutrition group(50%of their daily food requirement,UN group,n=25).The levels of maternal serum FGF23,tissue homogenate FGF23,and bone gla protein in fetal rats,and placental FGF23 mRNA and protein expression were examined by enzyme-linked immunosorbent assay,real-time qPCR analysis respectively.Finally,the effect of recombinant FGF23 on the viability of MG-63 cells was determined by cell proliferation assay.Data were analyzed with independent two-tailed t test and one-way analysis of variance.Spearman rank-order correlation coefficients(continuous variables)was performed to determine the relationship of results.Results:The diet restriction induced IUGR in rat offsprings,and the UN group exhibited a significantly lower FGF23 level(P<0.05,n=5).The FGF23 level was increased and peaked in maternal serum on gestation day(GD)15,but peaked in fetal and placenta on GD20.Moreover,the tissue homogenate levels of FGF23 and bone gla protein in fetal rats in both groups were positively correlated(r=0.923,P<0.05;r=0.925,P<0.05,respectively,n=15),FGF23 was localized to both decidual and labyrinth zones,with remarkably higher expression on GD20,P<0.05,n=5.In vitro,recombinant human FGF23 enhanced MG-63 cell viability,P<0.05,n=5.Conclusion:Prenatal undernutrition could decrease the FGF23 expression in fetal rats caused by the mother through the placenta,and induced the IUGR and hindered the ossification.And the FGF23 levels are peaked on GD15 mother but peaked on GD20 placenta and fetuses,these might be associated with the over compensation of maternal placenta on GD20.