Summary： Zinc finger of the cerebellum （ZIC 1）, one of ZIC family genes, has been shown to play im- portant roles in many cancers such as gastric cancer and breast cancer. However, there is little known about the e...Summary： Zinc finger of the cerebellum （ZIC 1）, one of ZIC family genes, has been shown to play im- portant roles in many cancers such as gastric cancer and breast cancer. However, there is little known about the expression and significance of ZIC1 in endometrial cancer. The aim of this study was to de- termine the expression pattern and clinicopathological significance of ZIC1 in endometrial cancer. The rnRNA and protein expression of ZIC1 in endometrial cancer tissues was detected using the reverse- transcription polymerase chain reaction and Western blotting, respectively. Immunostaining of ZIC1 in 99 endometrial cancer samples was examined and its associations with clinicopathological parameters were analyzed. Hec-l-B cells were transfected with Z1CI-shRNA or sc-shRNA, and cell proliferation was assayed. Hec-l-B cells stably transfected with ZICI-shRNA or sc-shRNA were subcutaneously in- oculated into nude mice, and the tumor weight was measured. A significantly increased expression of ZIC1 mRNA and protein was observed in endometrial cancer tissues compared to that in normal endo- metrial tissues （P〈0.05）. Immunohistochemical analysis showed that strong cytoplasmic immunostain- ing of ZIC1 was observed in almost all endometrial cancer samples （90/99） while light and moderate immunostaining of ZIC 1 was only detected in 17 of 30 （56.7%） normal tissues. Moreover, up-regulation of ZIC1 was significantly correlated with age, disease stage, TNM stage and FIGO stage （P〈0.05）. The down-regulated expression of ZIC1 contributed to the inhibition of cell proliferation, and inhibited the growth of tumor. It was concluded that ZIC1 is over-expressed in endometrial cancer tissue but not in normal tissue, and positively correlated to the malignant biological behavior of endometrial carcino- genesis.展开更多
Objective: To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and its receptor (TRH-R) in rat testis and their function in the reproduction and development. Methods: Total RNA of fat test...Objective: To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and its receptor (TRH-R) in rat testis and their function in the reproduction and development. Methods: Total RNA of fat testicular tissues was used as template. The oligonucleotide primers were designed from the sequence of rat hypothalamus prepro(pp)TRH and pituitary TRH-R cDNA for reverse transcription- polymerase chain reaction (RT- PCR ). Specific fragments of ppTRH and TRH-R cDNA were cloned and sequenced. Results: DNA sequence analysis indicated that cDNA Sequences of ppTRH and TRH-R from rat testis were consistent with those of hypothalamus ppTRH and pituitary TRH-R cDNA. Conclusion: TRH may function as a new autocrine or paracrine regulator of testicular function via a transmembrane receptor very similar or identical to that in pituitary.展开更多
文摘Summary： Zinc finger of the cerebellum （ZIC 1）, one of ZIC family genes, has been shown to play im- portant roles in many cancers such as gastric cancer and breast cancer. However, there is little known about the expression and significance of ZIC1 in endometrial cancer. The aim of this study was to de- termine the expression pattern and clinicopathological significance of ZIC1 in endometrial cancer. The rnRNA and protein expression of ZIC1 in endometrial cancer tissues was detected using the reverse- transcription polymerase chain reaction and Western blotting, respectively. Immunostaining of ZIC1 in 99 endometrial cancer samples was examined and its associations with clinicopathological parameters were analyzed. Hec-l-B cells were transfected with Z1CI-shRNA or sc-shRNA, and cell proliferation was assayed. Hec-l-B cells stably transfected with ZICI-shRNA or sc-shRNA were subcutaneously in- oculated into nude mice, and the tumor weight was measured. A significantly increased expression of ZIC1 mRNA and protein was observed in endometrial cancer tissues compared to that in normal endo- metrial tissues （P〈0.05）. Immunohistochemical analysis showed that strong cytoplasmic immunostain- ing of ZIC1 was observed in almost all endometrial cancer samples （90/99） while light and moderate immunostaining of ZIC 1 was only detected in 17 of 30 （56.7%） normal tissues. Moreover, up-regulation of ZIC1 was significantly correlated with age, disease stage, TNM stage and FIGO stage （P〈0.05）. The down-regulated expression of ZIC1 contributed to the inhibition of cell proliferation, and inhibited the growth of tumor. It was concluded that ZIC1 is over-expressed in endometrial cancer tissue but not in normal tissue, and positively correlated to the malignant biological behavior of endometrial carcino- genesis.
基金Supported by National Nature Science Foundation of China, No. 39870109
文摘Objective: To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and its receptor (TRH-R) in rat testis and their function in the reproduction and development. Methods: Total RNA of fat testicular tissues was used as template. The oligonucleotide primers were designed from the sequence of rat hypothalamus prepro(pp)TRH and pituitary TRH-R cDNA for reverse transcription- polymerase chain reaction (RT- PCR ). Specific fragments of ppTRH and TRH-R cDNA were cloned and sequenced. Results: DNA sequence analysis indicated that cDNA Sequences of ppTRH and TRH-R from rat testis were consistent with those of hypothalamus ppTRH and pituitary TRH-R cDNA. Conclusion: TRH may function as a new autocrine or paracrine regulator of testicular function via a transmembrane receptor very similar or identical to that in pituitary.
