Objective Reversed alkane δ;C and δ;H values in many prolific shale plays all over the world have aroused much attention in the study of the formation mechanism of reversed isotope series in alkanes in the past few ...Objective Reversed alkane δ;C and δ;H values in many prolific shale plays all over the world have aroused much attention in the study of the formation mechanism of reversed isotope series in alkanes in the past few years（Zou Caineng et al., 2016）. Although many researchers have put forward different hypotheses, the mechanism has not been well understood yet. The openness degree of oil and gas system展开更多
Objective To investigate the expression of growth factor Wnt 5A in urinary tumor cell lines. Methods By semi quantitative RT PCR (reverse transcriptase polymerase chain reaction), the expression of Wnt 5A in si...Objective To investigate the expression of growth factor Wnt 5A in urinary tumor cell lines. Methods By semi quantitative RT PCR (reverse transcriptase polymerase chain reaction), the expression of Wnt 5A in six urinary malignant cell lines, one primary cultured renal fibroblast and one case of normal human renal tissue was detected using Gel Doc 1000 computer controlled molecular analysis system. Results The expression of Wnt 5A in urinary tumor cell lines was much higher than that in primary cultured renal fibroblasts (PRF) and normal human renal tissue (NRT). The levels of Wnt 5A mRNA were different between six malignant cell lines. Conclusion The overexpression of growth factor Wnt 5A has a potential effect on the oncogenesis of urinary malignancies.展开更多
基金supported by the National Natural Science Foundation of China (grant No. 41503033)Basic Foresight Program of the Ministry of science and technology, Sinopec (grant No. G5800-15-ZS-KJB050-02)the Key Laboratory Project of Gansu Province (grant No. 1309RTSA041)
文摘Objective Reversed alkane δ;C and δ;H values in many prolific shale plays all over the world have aroused much attention in the study of the formation mechanism of reversed isotope series in alkanes in the past few years（Zou Caineng et al., 2016）. Although many researchers have put forward different hypotheses, the mechanism has not been well understood yet. The openness degree of oil and gas system
文摘Objective To investigate the expression of growth factor Wnt 5A in urinary tumor cell lines. Methods By semi quantitative RT PCR (reverse transcriptase polymerase chain reaction), the expression of Wnt 5A in six urinary malignant cell lines, one primary cultured renal fibroblast and one case of normal human renal tissue was detected using Gel Doc 1000 computer controlled molecular analysis system. Results The expression of Wnt 5A in urinary tumor cell lines was much higher than that in primary cultured renal fibroblasts (PRF) and normal human renal tissue (NRT). The levels of Wnt 5A mRNA were different between six malignant cell lines. Conclusion The overexpression of growth factor Wnt 5A has a potential effect on the oncogenesis of urinary malignancies.
