The melt treatment of A1-7%Si alloy by the flux injection method in a resistance furnance was investigated. The experimental results show that the flux injection method can realize the melt treatment of A1-7%Si alloy....The melt treatment of A1-7%Si alloy by the flux injection method in a resistance furnance was investigated. The experimental results show that the flux injection method can realize the melt treatment of A1-7%Si alloy. In purification, the flux injection method is superior to the standard lance degassing method. For the injection flux, the purifier Na2SiF6 is more powerful than Na3A1F6, the modifiers SrCl2, and R.E. Cl3 are quick acting, like NaF, but less powerful, the grain refiner containing K2TiF6 and KBF4 with Ti/B=1 is more potent than that with Ti/B=5. The analytical results indicate that the mass-transfer rate for the flux injection method is much higher than that for the conventional method .展开更多
目的探讨注射用重组人胰高血糖素类多肽-1(7-36)〔rhGLP-1(7-36)〕与二甲双胍合用治疗2型糖尿病(T2DM)的临床效果。方法采取随机、双盲、安慰剂对照的试验设计,36例T2DM患者在应用二甲双胍治疗的基础上随机分入用药组与安慰剂组,分别给...目的探讨注射用重组人胰高血糖素类多肽-1(7-36)〔rhGLP-1(7-36)〕与二甲双胍合用治疗2型糖尿病(T2DM)的临床效果。方法采取随机、双盲、安慰剂对照的试验设计,36例T2DM患者在应用二甲双胍治疗的基础上随机分入用药组与安慰剂组,分别给予皮下注射rhGLP-1(7-36)(0.2 mg)及安慰剂(甘露醇,2.5 mg)治疗12周;观察基线及12周后用药组与安慰剂组的糖化血红蛋白(HbA1C)、空腹血糖(FPG)及胰岛素水平(FINS)、餐后2 h静脉血糖(2 h PG)及胰岛素水平(2 h INS)、血脂、血压、体质量指数(BMI)、胰岛素抵抗指数(HOMA-IR)及胰岛素分泌指数(HOMA-IS)等指标的变化,同时记录低血糖事件及不良反应的发生情况。结果在应用二甲双胍治疗的基础上,用药组的T2DM患者在给予rhGLP-1(7-36)治疗12周后,HbA1C、FPG、2 h PG值以及体质量、BMI均比基线时有所降低,其差异具有统计学意义(P<0.05),低血糖发生率为2.8%。结论 rhGLP-1(7-36)联合二甲双胍治疗T2DM有效并且低血糖发生率低,可成为治疗新的选择。展开更多
Objective:To study the antipyretic and anti-inflammatory constituents from the active fraction of Reduning(RDN)Injection.Methods:In this study,the active fraction of RDN Injection was screened by the LPS-induced mouse...Objective:To study the antipyretic and anti-inflammatory constituents from the active fraction of Reduning(RDN)Injection.Methods:In this study,the active fraction of RDN Injection was screened by the LPS-induced mouse endotoxin shock model.The chemical constituents were isolated by chromatography on HP-20 macroporous adsorptive resins,silica gel,ODS columns and reverse phase MPLC and HPLC repeatedly,and their structures were elucidated based on spectroscopic analysis(HR-ESI-MS,NMR,ECD)and chemical methods.Meanwhile,we evaluated the anti-inflammatory activities of the isolates by measuring their inhibitory effects on TNF-αproduction in LPS stimulated RAW 264.7 macrophages.Results:The 95%ethanol eluate of RDN Injection by the macroporous adsorption resin column was proved to be the antipyretic and anti-inflammatory active fraction of this injection.A novel iridoid,named jasminoide A(1),and a new guaiane sesquiterpenoid,named(1 R,7 R,8 S,10 R)-7,8,11-trihydroxy-4-guaien-3-one(2),were isolated from Reduning injection,and compound 2 can inhibit TNF-αproduction with IC50 values of 72.24μmol/L.Conclusion:In this study,two new terpenoids were isolated from Reduning Injection,and compound 2 showed inhibitory activity against LPS-activated TNF-αproduction in RAW 264.7 cells in vitro.展开更多
文摘The melt treatment of A1-7%Si alloy by the flux injection method in a resistance furnance was investigated. The experimental results show that the flux injection method can realize the melt treatment of A1-7%Si alloy. In purification, the flux injection method is superior to the standard lance degassing method. For the injection flux, the purifier Na2SiF6 is more powerful than Na3A1F6, the modifiers SrCl2, and R.E. Cl3 are quick acting, like NaF, but less powerful, the grain refiner containing K2TiF6 and KBF4 with Ti/B=1 is more potent than that with Ti/B=5. The analytical results indicate that the mass-transfer rate for the flux injection method is much higher than that for the conventional method .
文摘目的探讨rhGLP-1(7-36)对糖尿病肾病(diabetic kidney disease,DKD)大鼠肾脏的保护机制。方法DKD模型大鼠随机分成模型组、rhGLP-1(7-36)低剂量组(20μg·kg^(-1))、rhGLP-1(7-36)中剂量组(40μg·kg^(-1))、rhGLP-1(7-36)高剂量组(80μg·kg^(-1)),另设正常组,皮下注射给药4周。全自动生化分析仪检测24 h尿微量白蛋白、血肌酐及尿肌酐平,计算肌酐清除率;进行腹腔糖耐量实验并计算药时曲线下面积(area under the cure,AUC);HE染色行肾组织病理形态学观察;Western blotting检测肾组织SIRT1蛋白水平。结果与模型组相比,rhGLP-1(7-36)高剂量组大鼠肌酐清除率有明显改善(P<0.05);24 h尿微量白蛋白水平明显下降(P<0.05);腹腔糖耐量AUC水平明显减小(P<0.05);肾组织病理变化有不同程度改善;肾组织SIRT1蛋白水平明显上升(P<0.05)。结论rhGLP-1(7-36)能有效改善DKD大鼠的肾脏功能,其机制可能与上调肾脏组织的SIRT1水平有关。
文摘目的探讨注射用重组人胰高血糖素类多肽-1(7-36)〔rhGLP-1(7-36)〕与二甲双胍合用治疗2型糖尿病(T2DM)的临床效果。方法采取随机、双盲、安慰剂对照的试验设计,36例T2DM患者在应用二甲双胍治疗的基础上随机分入用药组与安慰剂组,分别给予皮下注射rhGLP-1(7-36)(0.2 mg)及安慰剂(甘露醇,2.5 mg)治疗12周;观察基线及12周后用药组与安慰剂组的糖化血红蛋白(HbA1C)、空腹血糖(FPG)及胰岛素水平(FINS)、餐后2 h静脉血糖(2 h PG)及胰岛素水平(2 h INS)、血脂、血压、体质量指数(BMI)、胰岛素抵抗指数(HOMA-IR)及胰岛素分泌指数(HOMA-IS)等指标的变化,同时记录低血糖事件及不良反应的发生情况。结果在应用二甲双胍治疗的基础上,用药组的T2DM患者在给予rhGLP-1(7-36)治疗12周后,HbA1C、FPG、2 h PG值以及体质量、BMI均比基线时有所降低,其差异具有统计学意义(P<0.05),低血糖发生率为2.8%。结论 rhGLP-1(7-36)联合二甲双胍治疗T2DM有效并且低血糖发生率低,可成为治疗新的选择。
基金supported by the National Standard Research of Traditional Chinese Medicine for Reduning Injection(ZYBZHC-JS-31)National Standard Research of Traditional Chinese Medicine for Guzhi Fuling Capsule(ZYBZH-C-JS-28)grant from the National Natural Science Foundation of China(No.81602984)。
文摘Objective:To study the antipyretic and anti-inflammatory constituents from the active fraction of Reduning(RDN)Injection.Methods:In this study,the active fraction of RDN Injection was screened by the LPS-induced mouse endotoxin shock model.The chemical constituents were isolated by chromatography on HP-20 macroporous adsorptive resins,silica gel,ODS columns and reverse phase MPLC and HPLC repeatedly,and their structures were elucidated based on spectroscopic analysis(HR-ESI-MS,NMR,ECD)and chemical methods.Meanwhile,we evaluated the anti-inflammatory activities of the isolates by measuring their inhibitory effects on TNF-αproduction in LPS stimulated RAW 264.7 macrophages.Results:The 95%ethanol eluate of RDN Injection by the macroporous adsorption resin column was proved to be the antipyretic and anti-inflammatory active fraction of this injection.A novel iridoid,named jasminoide A(1),and a new guaiane sesquiterpenoid,named(1 R,7 R,8 S,10 R)-7,8,11-trihydroxy-4-guaien-3-one(2),were isolated from Reduning injection,and compound 2 can inhibit TNF-αproduction with IC50 values of 72.24μmol/L.Conclusion:In this study,two new terpenoids were isolated from Reduning Injection,and compound 2 showed inhibitory activity against LPS-activated TNF-αproduction in RAW 264.7 cells in vitro.
