Bacteria play critical roles in regulating soil phosphorus(P) cycling. The effects of interactions between crops and soil P-availability on bacterial communities and the feedback regulation of soil P cycling by the ba...Bacteria play critical roles in regulating soil phosphorus(P) cycling. The effects of interactions between crops and soil P-availability on bacterial communities and the feedback regulation of soil P cycling by the bacterial community modifications are poorly understood. Here, six soybean(Glycine max) genotypes with differences in P efficiency were cultivated in acidic soils with long-term sufficient or deficient P-fertilizer treatments. The acid phosphatase(AcP) activities, organic-P concentrations and associated bacterial community compositions were determined in bulk and rhizosphere soils. The results showed that both soybean plant P content and the soil AcP activity were negatively correlated with soil organic-P concentration in P-deficient acidic soils. Soil P-availability affected the ɑ-diversity of bacteria in both bulk and rhizosphere soils. However, soybean had a stronger effect on the bacterial community composition, as reflected by the similar biomarker bacteria in the rhizosphere soils in both P-treatments. The relative abundance of biomarker bacteria Proteobacteria was strongly correlated with soil organic-P concentration and AcP activity in low-P treatments. Further high-throughput sequencing of the phoC gene revealed an obvious shift in Proteobacteria groups between bulk soils and rhizosphere soils, which was emphasized by the higher relative abundances of Cupriavidus and Klebsiella, and lower relative abundance of Xanthomonas in rhizosphere soils. Among them, Cupriavidus was the dominant phoC bacterial genus, and it was negatively correlated with the soil organic-P concentration. These findings suggest that soybean growth relies on organic-P mineralization in P-deficient acidic soils, which might be partially achieved by recruiting specific phoCharboring bacteria, such as Cupriavidus.展开更多
Microbe communities in rhizosphere ecosystems are important for plant health but there is limited knowledge of them in the rhizospheres of genetically modified(GM) plants, especial for tree species. We used the ampl...Microbe communities in rhizosphere ecosystems are important for plant health but there is limited knowledge of them in the rhizospheres of genetically modified(GM) plants, especial for tree species. We used the amplitude sequencing method to analyze the V4 regions of the 16 S r RNA gene to identify changes in bacterial diversity and community structure in two GM lines(D520 and D521), one non-genetically modified(nonGM) line and in uncultivated soil. After chimera filtering,468.133 sequences in the domain Bacteria remained. There were ten dominant taxonomic groups(with [1 % of all sequences) across the samples. 241 of 551 genera(representing a ratio of 97.33 %) were common to all samples.A Venn diagram showed that 1.926 operational taxonomic units(OTUs) were shared by all samples. We found a specific change, a reduction in Chloroflexi, in the microorganisms in the rhizosphere soil planted with poplars. Taken together, the results showed few statistical differences in the bacterial diversity and community structure between the GM line and non-GM line, this suggests that there was no or very limited impact of this genetic modification on the bacterial communities in the rhizosphere.展开更多
Positive associations between alpine cushion plants and other species have been extensively studied.However,almost all studies have focused on the associations between macrofauna.Studies that have investigated positiv...Positive associations between alpine cushion plants and other species have been extensively studied.However,almost all studies have focused on the associations between macrofauna.Studies that have investigated positive associations between alpine cushion plants and rhizospheric microbes have been limited to the vegetation growing season.Here,we asked whether the positive effects that alpine cushion plants confer on rhizospheric microbe communities vary with seasons.We assessed seasonal variations in the bacterial diversity and composition in rhizosphere of two alpine cushion plants and surrounding bare ground by employing a high throughput sequencing method targeting the V3 region of bacterial 16 S rRNA genes.Soil properties of the rhizosphere and the bare ground were also examined.We found that cushion rhizospheres harbored significantly more C,N,S,ammonia nitrogen,and soil moisture than the bare ground.Soil properties in cushion rhizospheres were not notably different,except for soil pH.Bacterial diversities within the same microhabitats did not vary significantly with seasons.We concluded that alpine cushion plants had positive effects on the rhizospheric bacterial communities,even though the strength of the effect varied in different cushion species.Cushion species and the soil sulfur content were probably the major factors driving the spatial distribution and structure of soil bacterial communities in the alpine communities dominated by cushion plants.展开更多
Sulfate-reducing bacteria(SRB)are ubiquitous anaerobic microorganisms that play signifi cant roles in the global biogeochemical cycle.Coastal wetlands,one of the major habitats of SRB,exhibit high sulfate-reducing act...Sulfate-reducing bacteria(SRB)are ubiquitous anaerobic microorganisms that play signifi cant roles in the global biogeochemical cycle.Coastal wetlands,one of the major habitats of SRB,exhibit high sulfate-reducing activity and thus play signifi cant roles in organic carbon remineralization,benthic geochemical action,and plant-microbe interactions.Recent studies have provided credible evidence that the functional rather than the taxonomic composition of microbes responds more closely to environmental factors.Therefore,in this study,functional gene prediction based on PacBio single molecular real-time sequencing of 16S rDNA was applied to determine the sulfate-reducing and organic substrate-decomposing activities of SRB in the rhizospheres of two typical coastal wetland plants in North and South China:Zostera japonica and Scirpus mariqueter.To this end,some physicochemical characteristics of the sediments as well as the phylogenetic structure,community composition,diversity,and proportions of several functional genes of the SRB in the two plant rhizospheres were analyzed.The Z.japonic a meadow had a higher dissimilatory sulfate reduction capability than the S.mariqueter-comprising saltmarsh,owing to its larger proportion of SRB in the microbial community,larger proportions of functional genes involved in dissimilatory sulfate reduction,and the stronger ability of the SRB to degrade organic substrates completely.This study confi rmed the feasibility of applying microbial community function prediction in research on the metabolic features of SRB,which will be helpful for gaining new knowledge of the biogeochemical and ecological roles of these bacteria in coastal wetlands.展开更多
A two-year field experiment conducted under dryland conditions in semi-humid and drought-prone regions of China aimed to assess the effect of ammonia-oxidizing bacterial on maize water use efficiency and yield.A heter...A two-year field experiment conducted under dryland conditions in semi-humid and drought-prone regions of China aimed to assess the effect of ammonia-oxidizing bacterial on maize water use efficiency and yield.A heterotrophic ammonia-oxidizing bacteria(HAOB)strain S2_8_1 was used.Six treatments were applied:(1)no irrigation+HAOB strain(DI),(2)no irrigation+blank culture medium(DM),(3)no irrigation control(DCK),(4)irrigation+HAOB(WI),(5)irrigation+blank culture medium(WM),and(6)irrigation control(WCK).Results revealed that HAOB treatment increased maize growth,yield,and water use efficiency over controls,regardless of whether the year was wet or dry.This improvement was attributed to the accelerated nitrification in the rhizosphere soil due to HAOB inoculation,which subsequently led to increased levels of leaf cytokinins.Overall,these findings suggest that HAOB inoculation holds promise as a strategy to boost water use efficiency and maize productivity in dryland agriculture.展开更多
Phthalic acid is a main pollutant, which is also an important reason for the continuous cropping effect of tobacco. In order to degrade the phthalic acid accumulated in the environment and relieve the obstacle effect ...Phthalic acid is a main pollutant, which is also an important reason for the continuous cropping effect of tobacco. In order to degrade the phthalic acid accumulated in the environment and relieve the obstacle effect of tobacco continuous cropping caused by the accumulation of phthalic acid in the soil. In this study, phthalate degrading bacteria B3 is screened from continuous cropping tobacco soil. The results of biochemical identification and 16sDNA comparison show that the homology between degrading bacterium B3 and Enterobacter sp. is 99%. At the same time, the growth of Enterobacter hormaechei subsp. B3 and the degradation of phthalic acid under different environmental conditions are studied. The results show that the environment with a temperature of 30˚C, PH of 7, and inoculation amount of not less than 1.2%, which is the optimal growth conditions for Enterobacter sp. B3. In an environment with a concentration of phthalic acid not exceeding 500 mg/L, Enterobacter sp. B3 has a better effect on phthalic acid degradation, and the degradation rate can reach 77% in 7 d. The results of indoor potting experiments on tobacco show that the degradation rate of phthalic acid by Enterobacter B3 in the soil is about 45%, which can reduce the inhibitory effect of phthalic acid on the growth of tobacco seedlings. This study enriches the microbial resources for degrading phthalic acid and provides a theoretical basis for alleviating tobacco continuous cropping obstacles.展开更多
Microbial diversity has been an important facet of scientific research, since microbes promise a plethora of biomolecules which are otherwise not found in nature. Microbes are subjected to high level of competition fo...Microbial diversity has been an important facet of scientific research, since microbes promise a plethora of biomolecules which are otherwise not found in nature. Microbes are subjected to high level of competition for survival in the environment, and hence develop mechanisms of defense. The biomolecules produced by these microbes as part of their defense or survival mechanism, are of importance for human and animal drugs and many other industrial and environmental applications. The marine counterparts of these terrestrial microbes have yet higher potential, since the marine environment has higher biotic and abiotic stresses, leading to new molecule discovery. In the current study, a bacterial diversity study of the culturable bacteria of the mangrove rhizosphere of Avicennia marina has been undertaken, to understand the flora diversity. Mangroves are unique ecosystems which are under a combination of marine and terrestrial influence. Mangroves are seaward, inland and also found in creek areas. This diversity in their habitat, leads them to produce variable root exudates, which support the growth of different types of organisms. This study has revealed that certain species are dominant in these ecosystems irrespective of the biotic and abiotic stresses, whereas certain species appear only at neutral pH. The study will help select organisms for further biomolecule discovery programs, based on their environment of isolation and other growth parameters.展开更多
Incessant application of chemical fertilizers to the agricultural fields may alter the composition and activities of soil microbiota.Thus,the shift of cultivation practices from chemical to organic is considered to be...Incessant application of chemical fertilizers to the agricultural fields may alter the composition and activities of soil microbiota.Thus,the shift of cultivation practices from chemical to organic is considered to be the need of the hour in order to maintain soil health.A study was conducted in the agricultural fields of the University of Burdwan,India to observe the impact of organic manure on the rhizosphere bacterial community.The experiments were conducted on maize plants,supplemented with the recommended dose of chemical fertilizer and organic manure(vermicompost and cow dung mixture).Corresponding changes in the plant phenological events and soil health in terms of soil physico-chemical factors and rhizosphere bacterial groups up to the level of CFU g-1×105 dry soil was noted.The results showed a significant increase in population of phosphate solubilizing bacteria during 30DAS.However,at 90 DAS,significant increase in the population of phosphate solubilizing bacteria,nitrifying bacteria,asymbiotic nitrogen-fixing bacteria and protein hydrolyzing bacteria was observed in the organically treated plots.The growth of rhizosphere bacteria was attributed to the type of organic manure supplied to the agricultural fields.In addition,a strong correlation was observed between Zn and protein hydrolyzing bacteria.The soil organic carbon and available nitrogen were strongly correlated with nitrifying,fat solubilizing and phosphate solubilizing groups of bacteria.展开更多
Two rice cultivars(Xiushui 09 and Chunyou 84)were used to evaluate the effects of various soil oxygen(O2)conditions on soil nitrogen(N)transformation,absorption and accumulation in rice plants.The treatments were cont...Two rice cultivars(Xiushui 09 and Chunyou 84)were used to evaluate the effects of various soil oxygen(O2)conditions on soil nitrogen(N)transformation,absorption and accumulation in rice plants.The treatments were continuous flooding(CF),continuous flooding and aeration(CFA),and alternate wetting and drying(AWD).The results showed that the AWD and CFA treatments improved soil N transformation,rice growth,and N absorption and accumulation.Soil NO3–content,nitrification activity and ammonia-oxidising bacteria abundance,leaf area,nitrate reductase activity,and N absorption and accumulation in rice all increased in both cultivars.However,soil microbial biomass carbon and pH did not significantly change during the whole period of rice growth.Correlation analysis revealed a significant positive correlation between the nitrification activity and ammonia-oxidising bacteria abundance,and both of them significantly increased as the total N accumulation in rice increased.Our results indicated that improved soil O2 conditions led to changing soil N cycling and contributed to increases in N absorption and accumulation by rice in paddy fields.展开更多
基金This work was supported by grants from the National Key Research and Development Program of China(2021YFF1000500)the Open Competition Program of Ten Major Directions of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province,China(2022SDZG07)+3 种基金the Key Areas Research and Development Programs of Guangdong Province,China(2022B0202060005)the STICGrantof China(SGDX20210823103535007)the Major Program of Guangdong Basic and Applied Research,China(2019B030302006)the Natural Science Foundation of Guangdong Province,China(2021A1515010826and 2020A1515110261).
文摘Bacteria play critical roles in regulating soil phosphorus(P) cycling. The effects of interactions between crops and soil P-availability on bacterial communities and the feedback regulation of soil P cycling by the bacterial community modifications are poorly understood. Here, six soybean(Glycine max) genotypes with differences in P efficiency were cultivated in acidic soils with long-term sufficient or deficient P-fertilizer treatments. The acid phosphatase(AcP) activities, organic-P concentrations and associated bacterial community compositions were determined in bulk and rhizosphere soils. The results showed that both soybean plant P content and the soil AcP activity were negatively correlated with soil organic-P concentration in P-deficient acidic soils. Soil P-availability affected the ɑ-diversity of bacteria in both bulk and rhizosphere soils. However, soybean had a stronger effect on the bacterial community composition, as reflected by the similar biomarker bacteria in the rhizosphere soils in both P-treatments. The relative abundance of biomarker bacteria Proteobacteria was strongly correlated with soil organic-P concentration and AcP activity in low-P treatments. Further high-throughput sequencing of the phoC gene revealed an obvious shift in Proteobacteria groups between bulk soils and rhizosphere soils, which was emphasized by the higher relative abundances of Cupriavidus and Klebsiella, and lower relative abundance of Xanthomonas in rhizosphere soils. Among them, Cupriavidus was the dominant phoC bacterial genus, and it was negatively correlated with the soil organic-P concentration. These findings suggest that soybean growth relies on organic-P mineralization in P-deficient acidic soils, which might be partially achieved by recruiting specific phoCharboring bacteria, such as Cupriavidus.
基金financially supported by the National High-Tech Research and Development Program of China,863Program(Grant No.2011AA100201)the National Forestry Public Welfare Research Project of China(Grant No.201004004)
文摘Microbe communities in rhizosphere ecosystems are important for plant health but there is limited knowledge of them in the rhizospheres of genetically modified(GM) plants, especial for tree species. We used the amplitude sequencing method to analyze the V4 regions of the 16 S r RNA gene to identify changes in bacterial diversity and community structure in two GM lines(D520 and D521), one non-genetically modified(nonGM) line and in uncultivated soil. After chimera filtering,468.133 sequences in the domain Bacteria remained. There were ten dominant taxonomic groups(with [1 % of all sequences) across the samples. 241 of 551 genera(representing a ratio of 97.33 %) were common to all samples.A Venn diagram showed that 1.926 operational taxonomic units(OTUs) were shared by all samples. We found a specific change, a reduction in Chloroflexi, in the microorganisms in the rhizosphere soil planted with poplars. Taken together, the results showed few statistical differences in the bacterial diversity and community structure between the GM line and non-GM line, this suggests that there was no or very limited impact of this genetic modification on the bacterial communities in the rhizosphere.
基金supported by the National Key Research and Development Program of China(Grant No.2017YFC0505200,to Hang Sun)the Major Program of National Natural Science Foundation of China(Grant No.31590823,to Hang Sun)+2 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA20050203,to Hang Sun)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB15020302,to Jianqiang Su)the Yunnan Applied Basic Research Project(Grant No.2018FA015,to Yang Yang)
文摘Positive associations between alpine cushion plants and other species have been extensively studied.However,almost all studies have focused on the associations between macrofauna.Studies that have investigated positive associations between alpine cushion plants and rhizospheric microbes have been limited to the vegetation growing season.Here,we asked whether the positive effects that alpine cushion plants confer on rhizospheric microbe communities vary with seasons.We assessed seasonal variations in the bacterial diversity and composition in rhizosphere of two alpine cushion plants and surrounding bare ground by employing a high throughput sequencing method targeting the V3 region of bacterial 16 S rRNA genes.Soil properties of the rhizosphere and the bare ground were also examined.We found that cushion rhizospheres harbored significantly more C,N,S,ammonia nitrogen,and soil moisture than the bare ground.Soil properties in cushion rhizospheres were not notably different,except for soil pH.Bacterial diversities within the same microhabitats did not vary significantly with seasons.We concluded that alpine cushion plants had positive effects on the rhizospheric bacterial communities,even though the strength of the effect varied in different cushion species.Cushion species and the soil sulfur content were probably the major factors driving the spatial distribution and structure of soil bacterial communities in the alpine communities dominated by cushion plants.
基金Supported by the Scientifi c Research Fund of the Second Institute of Oceanography,Ministry of Natural Resources(MNR)(Nos.JB1906,JG1616,JG1910)the Zhejiang Qingshan Lake Innovation Platform for Marine Science and Technology(No.2017E80001)+4 种基金the Key Projects of Philosophy and Social Sciences Research,Ministry of Education(No.18JZD059)the National Key Technology Research and Development Program of the Ministry of Science and Technology of the China(No.2015BAD08B01)the State Key Laboratory of Satellite Ocean Environment Dynamics(No.SOEDZZ1902)the National Natural Science Foundation of China(No.41806136)the Project of Long Term Observation and Research Plan in the Changjiang Estuary and the Adjacent East China Sea(LORCE,14282)。
文摘Sulfate-reducing bacteria(SRB)are ubiquitous anaerobic microorganisms that play signifi cant roles in the global biogeochemical cycle.Coastal wetlands,one of the major habitats of SRB,exhibit high sulfate-reducing activity and thus play signifi cant roles in organic carbon remineralization,benthic geochemical action,and plant-microbe interactions.Recent studies have provided credible evidence that the functional rather than the taxonomic composition of microbes responds more closely to environmental factors.Therefore,in this study,functional gene prediction based on PacBio single molecular real-time sequencing of 16S rDNA was applied to determine the sulfate-reducing and organic substrate-decomposing activities of SRB in the rhizospheres of two typical coastal wetland plants in North and South China:Zostera japonica and Scirpus mariqueter.To this end,some physicochemical characteristics of the sediments as well as the phylogenetic structure,community composition,diversity,and proportions of several functional genes of the SRB in the two plant rhizospheres were analyzed.The Z.japonic a meadow had a higher dissimilatory sulfate reduction capability than the S.mariqueter-comprising saltmarsh,owing to its larger proportion of SRB in the microbial community,larger proportions of functional genes involved in dissimilatory sulfate reduction,and the stronger ability of the SRB to degrade organic substrates completely.This study confi rmed the feasibility of applying microbial community function prediction in research on the metabolic features of SRB,which will be helpful for gaining new knowledge of the biogeochemical and ecological roles of these bacteria in coastal wetlands.
基金supported by the National Natural Science Foundation of China(U1304326)the Natural Science Foundation of Henan Provincial(242300421242)。
文摘A two-year field experiment conducted under dryland conditions in semi-humid and drought-prone regions of China aimed to assess the effect of ammonia-oxidizing bacterial on maize water use efficiency and yield.A heterotrophic ammonia-oxidizing bacteria(HAOB)strain S2_8_1 was used.Six treatments were applied:(1)no irrigation+HAOB strain(DI),(2)no irrigation+blank culture medium(DM),(3)no irrigation control(DCK),(4)irrigation+HAOB(WI),(5)irrigation+blank culture medium(WM),and(6)irrigation control(WCK).Results revealed that HAOB treatment increased maize growth,yield,and water use efficiency over controls,regardless of whether the year was wet or dry.This improvement was attributed to the accelerated nitrification in the rhizosphere soil due to HAOB inoculation,which subsequently led to increased levels of leaf cytokinins.Overall,these findings suggest that HAOB inoculation holds promise as a strategy to boost water use efficiency and maize productivity in dryland agriculture.
文摘Phthalic acid is a main pollutant, which is also an important reason for the continuous cropping effect of tobacco. In order to degrade the phthalic acid accumulated in the environment and relieve the obstacle effect of tobacco continuous cropping caused by the accumulation of phthalic acid in the soil. In this study, phthalate degrading bacteria B3 is screened from continuous cropping tobacco soil. The results of biochemical identification and 16sDNA comparison show that the homology between degrading bacterium B3 and Enterobacter sp. is 99%. At the same time, the growth of Enterobacter hormaechei subsp. B3 and the degradation of phthalic acid under different environmental conditions are studied. The results show that the environment with a temperature of 30˚C, PH of 7, and inoculation amount of not less than 1.2%, which is the optimal growth conditions for Enterobacter sp. B3. In an environment with a concentration of phthalic acid not exceeding 500 mg/L, Enterobacter sp. B3 has a better effect on phthalic acid degradation, and the degradation rate can reach 77% in 7 d. The results of indoor potting experiments on tobacco show that the degradation rate of phthalic acid by Enterobacter B3 in the soil is about 45%, which can reduce the inhibitory effect of phthalic acid on the growth of tobacco seedlings. This study enriches the microbial resources for degrading phthalic acid and provides a theoretical basis for alleviating tobacco continuous cropping obstacles.
文摘Microbial diversity has been an important facet of scientific research, since microbes promise a plethora of biomolecules which are otherwise not found in nature. Microbes are subjected to high level of competition for survival in the environment, and hence develop mechanisms of defense. The biomolecules produced by these microbes as part of their defense or survival mechanism, are of importance for human and animal drugs and many other industrial and environmental applications. The marine counterparts of these terrestrial microbes have yet higher potential, since the marine environment has higher biotic and abiotic stresses, leading to new molecule discovery. In the current study, a bacterial diversity study of the culturable bacteria of the mangrove rhizosphere of Avicennia marina has been undertaken, to understand the flora diversity. Mangroves are unique ecosystems which are under a combination of marine and terrestrial influence. Mangroves are seaward, inland and also found in creek areas. This diversity in their habitat, leads them to produce variable root exudates, which support the growth of different types of organisms. This study has revealed that certain species are dominant in these ecosystems irrespective of the biotic and abiotic stresses, whereas certain species appear only at neutral pH. The study will help select organisms for further biomolecule discovery programs, based on their environment of isolation and other growth parameters.
文摘Incessant application of chemical fertilizers to the agricultural fields may alter the composition and activities of soil microbiota.Thus,the shift of cultivation practices from chemical to organic is considered to be the need of the hour in order to maintain soil health.A study was conducted in the agricultural fields of the University of Burdwan,India to observe the impact of organic manure on the rhizosphere bacterial community.The experiments were conducted on maize plants,supplemented with the recommended dose of chemical fertilizer and organic manure(vermicompost and cow dung mixture).Corresponding changes in the plant phenological events and soil health in terms of soil physico-chemical factors and rhizosphere bacterial groups up to the level of CFU g-1×105 dry soil was noted.The results showed a significant increase in population of phosphate solubilizing bacteria during 30DAS.However,at 90 DAS,significant increase in the population of phosphate solubilizing bacteria,nitrifying bacteria,asymbiotic nitrogen-fixing bacteria and protein hydrolyzing bacteria was observed in the organically treated plots.The growth of rhizosphere bacteria was attributed to the type of organic manure supplied to the agricultural fields.In addition,a strong correlation was observed between Zn and protein hydrolyzing bacteria.The soil organic carbon and available nitrogen were strongly correlated with nitrifying,fat solubilizing and phosphate solubilizing groups of bacteria.
基金the National Key Research and Development Program of China(Grant No.2016YFD300507)the National Natural Science Foundation of China(Grant No.31401343)the National Rice Industry Technology System of China(Grant No.CARS-01-04A).
文摘Two rice cultivars(Xiushui 09 and Chunyou 84)were used to evaluate the effects of various soil oxygen(O2)conditions on soil nitrogen(N)transformation,absorption and accumulation in rice plants.The treatments were continuous flooding(CF),continuous flooding and aeration(CFA),and alternate wetting and drying(AWD).The results showed that the AWD and CFA treatments improved soil N transformation,rice growth,and N absorption and accumulation.Soil NO3–content,nitrification activity and ammonia-oxidising bacteria abundance,leaf area,nitrate reductase activity,and N absorption and accumulation in rice all increased in both cultivars.However,soil microbial biomass carbon and pH did not significantly change during the whole period of rice growth.Correlation analysis revealed a significant positive correlation between the nitrification activity and ammonia-oxidising bacteria abundance,and both of them significantly increased as the total N accumulation in rice increased.Our results indicated that improved soil O2 conditions led to changing soil N cycling and contributed to increases in N absorption and accumulation by rice in paddy fields.