Purpose:The aim of this study was to examine systemic responses of oxidant/antioxidant status following 2 training sessions of different intensity in amateur rhythmic gymnasts.Methods:Before the experimental training,...Purpose:The aim of this study was to examine systemic responses of oxidant/antioxidant status following 2 training sessions of different intensity in amateur rhythmic gymnasts.Methods:Before the experimental training,10 female gymnasts performed a gradually increased exercise test to assess maximal heart rate,maximal oxygen consumption,and anaerobic threshold.They executed 2 intermittent training sessions separated by 48 h of recovery(48 h-post R):the first was performed at low-moderate intensity(LMI)and the second at high intensity(HI).Blood samples were collected immediately preand post-training and 48 h-post R.Hydroperoxide level(OxL)and total antioxidant capacity(TAC)were photometrically measured.Results:OxL was significantly higher in post-training and 48 h-post R following HI than the same conditions after an LMI session(HI vs.LMI post-training:381.10±46.17(mean±SD)vs.344.18±27.94 Units Carratelli(U.CARR);48 h-post R:412.21±26.61 vs.373.80±36.08 U.CARR).There was no change in TAC between the 2 training sessions investigated.In LMI training,OxL significantly decreased in post-training and increased to reach the baseline at 48 h-post R,whereas TAC increased only at 48 h-post R.In HI training,OxL significantly increased to reach a high oxidative stress 48 h-post R,whereas TAC was lower in post-training than pre-training.Conclusion:The pattern of OxL and TAC levels implies different regulation mechanisms by HI and LMI training sessions.High oxidative stress induced by an HI protocol might be associated with both insufficient TAC and recovery time at 48 h necessary to restore redox balance.展开更多
The Mongolian gerbil has been widely used in many research fields and has been reported to be a diurnal laboratory animal. The circadian rhythmicity of these gerbils was investigated in the present study by measuring ...The Mongolian gerbil has been widely used in many research fields and has been reported to be a diurnal laboratory animal. The circadian rhythmicity of these gerbils was investigated in the present study by measuring two hormones that show daily oscillations, cortisol and ACTH, in serum using ELISA kits. The levels of the two hormones were highest at 8:00 am and their rhythmic changes were similar to those in humans. In addition, the influence of stress of handling and blood collection on the physiological parameters of the gerbils was examined. After adaptation to handling for 1 week, some serum parameters in the animals changed. Handling and blood collection did not impact significantly on the following parameters: creatine kinase (CK), lactate dehydrogenase (LD), alanine aminotransferase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), and albumin (ALB). However, blood glucose (GLU), total protein (TP) and globulin (GLB) significantly increased while creatinine (CRE) and albumin/globulin (A/G) significantly decreased after adaptation. This work further confirms that the Mongolian gerbil is a diurnal animal and also indicates that a suitable adaptation procedure is necessary for getting reliable results when performing experiments using these animals.展开更多
基金supported by the University of Palermo (FFR 2012-13)
文摘Purpose:The aim of this study was to examine systemic responses of oxidant/antioxidant status following 2 training sessions of different intensity in amateur rhythmic gymnasts.Methods:Before the experimental training,10 female gymnasts performed a gradually increased exercise test to assess maximal heart rate,maximal oxygen consumption,and anaerobic threshold.They executed 2 intermittent training sessions separated by 48 h of recovery(48 h-post R):the first was performed at low-moderate intensity(LMI)and the second at high intensity(HI).Blood samples were collected immediately preand post-training and 48 h-post R.Hydroperoxide level(OxL)and total antioxidant capacity(TAC)were photometrically measured.Results:OxL was significantly higher in post-training and 48 h-post R following HI than the same conditions after an LMI session(HI vs.LMI post-training:381.10±46.17(mean±SD)vs.344.18±27.94 Units Carratelli(U.CARR);48 h-post R:412.21±26.61 vs.373.80±36.08 U.CARR).There was no change in TAC between the 2 training sessions investigated.In LMI training,OxL significantly decreased in post-training and increased to reach the baseline at 48 h-post R,whereas TAC increased only at 48 h-post R.In HI training,OxL significantly increased to reach a high oxidative stress 48 h-post R,whereas TAC was lower in post-training than pre-training.Conclusion:The pattern of OxL and TAC levels implies different regulation mechanisms by HI and LMI training sessions.High oxidative stress induced by an HI protocol might be associated with both insufficient TAC and recovery time at 48 h necessary to restore redox balance.
基金National Natural Science Foundation of China,Grant/Award Number:No.31572341
文摘The Mongolian gerbil has been widely used in many research fields and has been reported to be a diurnal laboratory animal. The circadian rhythmicity of these gerbils was investigated in the present study by measuring two hormones that show daily oscillations, cortisol and ACTH, in serum using ELISA kits. The levels of the two hormones were highest at 8:00 am and their rhythmic changes were similar to those in humans. In addition, the influence of stress of handling and blood collection on the physiological parameters of the gerbils was examined. After adaptation to handling for 1 week, some serum parameters in the animals changed. Handling and blood collection did not impact significantly on the following parameters: creatine kinase (CK), lactate dehydrogenase (LD), alanine aminotransferase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), and albumin (ALB). However, blood glucose (GLU), total protein (TP) and globulin (GLB) significantly increased while creatinine (CRE) and albumin/globulin (A/G) significantly decreased after adaptation. This work further confirms that the Mongolian gerbil is a diurnal animal and also indicates that a suitable adaptation procedure is necessary for getting reliable results when performing experiments using these animals.
