[ Objective] This study was to breed rice cultivars with multi-resistance to Orseolia oryzae （Wood-Mason）. [ Method] The Guangxi local cultivar GX-M001 （Jiangchao） with high resistance to Orseolia oryzae （Wood-Ma...[ Objective] This study was to breed rice cultivars with multi-resistance to Orseolia oryzae （Wood-Mason）. [ Method] The Guangxi local cultivar GX-M001 （Jiangchao） with high resistance to Orseolia oryzae （Wood-Mason） was used to hybrid with the known resistance cultivars ＂Kangwenqingzhan＂ （harboring GM5 gene）, OB677（ harboring GM3 gene） from Sri Lanka, HT1350 and high yield end quality cultivar ＂ Guiruanzhan＂. [ Result] Through pyramiding the multi-resistant genes via routine hybridization, the general resistances of the hybrids were remarkably enhanced. The grades of resistance were also improved, many of the combinations were endowed with a resistance at immune level （grade 0） ; and interestingly, the respective hybridization of GX-M001 （high resistance） with OB677（ medium resistance） and HT1350（suscepti- ble） also generate two lines at immune level, which is probably the effects of additive effects of genes.[ Conclusion] By routine hybridization, multiple genes were successfully pyramided, thus generating novel rice lines with multiple resistances. For the rice breeding scientists at the grass-roots level, the resistance-resistance pyramiding is an effective approach to breed high resistance cultivars.展开更多
The research results of marker aided selection(MAS)for resistant varieties and lines against rice gall midge Orseolia oryzae Wood-Mason successfully in 1999 - 2002 were reported in the present paper. The molecular mar...The research results of marker aided selection(MAS)for resistant varieties and lines against rice gall midge Orseolia oryzae Wood-Mason successfully in 1999 - 2002 were reported in the present paper. The molecular markers linked to the gene Gm6 against rice gall midge were used to select and breed the resistant varieties and lines. The RAPD marker OPM06 was used to verify the existence actually of gene Gm6 in ten developed varieties resistant to gall midge such as Duokang1, Duokang2, Kangwen2, Kangwen3, Kang-wen5, Duokangzaozhan, Kangwenqinzhan, which were derived from Daqiuqi. For resistance breeding through PCRbased marker aided selection(MAS), the polymorphisms in the resistant and susceptible parents were i-dentified by RG476/Alu I and RG476/Sca I respectively. The RAPD marker OPM06(1.4 kb)was used to i-dentify 15 new resistance lines from F3 lines of Fengyinzhan1/Daqiuqi in 1999. 21 and 7 resistance lines were selected from F4 and F6 lines of KWQZ/Gui99(restored line of hybrid rice)using RG476/Alu I in 2000-2001 respectively. The Gm6 gene was transferred into the restored line of hybrid rice. In 2001 - 2002, RG214/ Hha I and G214/Sca I were used for selecting 11 and 5 resistance lines from F3 lines of KWQZ/IR56 and AXZ/KWQZ successfully. The application of the resistance gene through PCR-based marker aided selection is a new and effective approach in resistance breeding.展开更多
基金Supported by National Natural Science Foundation of China(30760117)National Key Technology R &D Program (2007BAD68B01)~~
文摘[ Objective] This study was to breed rice cultivars with multi-resistance to Orseolia oryzae （Wood-Mason）. [ Method] The Guangxi local cultivar GX-M001 （Jiangchao） with high resistance to Orseolia oryzae （Wood-Mason） was used to hybrid with the known resistance cultivars ＂Kangwenqingzhan＂ （harboring GM5 gene）, OB677（ harboring GM3 gene） from Sri Lanka, HT1350 and high yield end quality cultivar ＂ Guiruanzhan＂. [ Result] Through pyramiding the multi-resistant genes via routine hybridization, the general resistances of the hybrids were remarkably enhanced. The grades of resistance were also improved, many of the combinations were endowed with a resistance at immune level （grade 0） ; and interestingly, the respective hybridization of GX-M001 （high resistance） with OB677（ medium resistance） and HT1350（suscepti- ble） also generate two lines at immune level, which is probably the effects of additive effects of genes.[ Conclusion] By routine hybridization, multiple genes were successfully pyramided, thus generating novel rice lines with multiple resistances. For the rice breeding scientists at the grass-roots level, the resistance-resistance pyramiding is an effective approach to breed high resistance cultivars.
文摘The research results of marker aided selection(MAS)for resistant varieties and lines against rice gall midge Orseolia oryzae Wood-Mason successfully in 1999 - 2002 were reported in the present paper. The molecular markers linked to the gene Gm6 against rice gall midge were used to select and breed the resistant varieties and lines. The RAPD marker OPM06 was used to verify the existence actually of gene Gm6 in ten developed varieties resistant to gall midge such as Duokang1, Duokang2, Kangwen2, Kangwen3, Kang-wen5, Duokangzaozhan, Kangwenqinzhan, which were derived from Daqiuqi. For resistance breeding through PCRbased marker aided selection(MAS), the polymorphisms in the resistant and susceptible parents were i-dentified by RG476/Alu I and RG476/Sca I respectively. The RAPD marker OPM06(1.4 kb)was used to i-dentify 15 new resistance lines from F3 lines of Fengyinzhan1/Daqiuqi in 1999. 21 and 7 resistance lines were selected from F4 and F6 lines of KWQZ/Gui99(restored line of hybrid rice)using RG476/Alu I in 2000-2001 respectively. The Gm6 gene was transferred into the restored line of hybrid rice. In 2001 - 2002, RG214/ Hha I and G214/Sca I were used for selecting 11 and 5 resistance lines from F3 lines of KWQZ/IR56 and AXZ/KWQZ successfully. The application of the resistance gene through PCR-based marker aided selection is a new and effective approach in resistance breeding.
基金This research were supported by the Asia Rice Biotechnology Net Work project -Achieving Impact and Sus-tainabilitysponsored by Asian Development Bank. And the International cooperation project-studies on the resistance gene withmolecular biotechnologysupported by the Guangdong Province Commission of Science and Technology of China and Guangdong A-cademy of Agricultural Sciences.
