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西瓜TALE基因家族鉴定及表达分析
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作者 陈筱瑶 冯梦娇 +7 位作者 黄欣 张瑞青 代雪 王家发 张显 李军华 霍治邦 魏春华 《中国瓜菜》 CAS 北大核心 2024年第10期18-29,共12页
TALE基因家族在调控花器官发育、花分生组织形成、器官形态发生、果实发育、植物非生物胁迫响应及激素响应中发挥重要作用。利用生物信息学的方法鉴定了西瓜TALE基因家族成员,并分析了其基因结构、系统发育树、保守基序、顺式作用元件... TALE基因家族在调控花器官发育、花分生组织形成、器官形态发生、果实发育、植物非生物胁迫响应及激素响应中发挥重要作用。利用生物信息学的方法鉴定了西瓜TALE基因家族成员,并分析了其基因结构、系统发育树、保守基序、顺式作用元件、组织特异性表达,以及低温胁迫和干旱胁迫后基因的表达情况,为西瓜TALE基因家族的功能研究和利用提供了理论依据。结果表明,西瓜TALE基因家族共包含19个成员。系统发育分析将ClTALE家族分为BEL1-like和KNOX两个亚家族,KNOX亚家族又可进一步划分为两个分支ClassⅠ和ClassⅡ。顺式作用元件分析表明,ClTALE基因的启动子中富含光、植物激素和非生物胁迫响应的顺式元件,其中光响应元件最多。西瓜组织特异性表达分析表明,ClBLH09在根中高表达,ClBLH01在卷须、雌花和雄花中高表达,因此西瓜TALE基因家族可能参与了西瓜的生长发育过程。低温胁迫(0、3、6、12、24、36和48 h)和干旱胁迫(0、1、2、3、4、5、6、7和8 d)不同时间后基因的表达分析表明,ClBLH08和ClKN02在低温胁迫下呈现上调趋势,ClBLH04和ClBLH05在干旱胁迫下呈上调的趋势,因此西瓜TALE基因家族可能在低温胁迫和干旱胁迫中发挥作用。 展开更多
关键词 西瓜 tale基因家族 生物信息学 表达分析
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When Zhejiang Folklore Meets Andersen's Fairy Tales
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作者 Zheng Wen 《China & The World Cultural Exchange》 2023年第9期37-39,共3页
From August 22 to 24,2023,the exhibition"Moment in Jiangnan-When Zhejiang FolkloreMeets Andersen's FairyTales",hostedby China Cultural Center in Copenhagen and the Zhejiang Provincial Department of Cultu... From August 22 to 24,2023,the exhibition"Moment in Jiangnan-When Zhejiang FolkloreMeets Andersen's FairyTales",hostedby China Cultural Center in Copenhagen and the Zhejiang Provincial Department of Culture and TEMPY Tourism and organized by the Zhejiang Provincial Cultural Center. 展开更多
关键词 ANDERSEN tale ZHEJIANG
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A Tale of Two Continents-An Autobiography
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作者 Balmukunda Regmi 《International Understanding》 2024年第1期50-51,共2页
On 14th September 2023,we were gathered in a Beijing conference hall to exchange views with the leaders of the Chinese Association for International Understanding.We had been introduced about the Association by resear... On 14th September 2023,we were gathered in a Beijing conference hall to exchange views with the leaders of the Chinese Association for International Understanding.We had been introduced about the Association by researcher Zhang Yaowu during our field trips.However,when we met Ai Ping,I had no idea that we were talking with the author of"A Tale of Two Continents:An Autobiography".In receiving his signed book,I realized that the speaker was the current Vice-President of the Chinese Association for International Understanding and a Deputy Minister and an Ambassador Extraordinary and Plenipotentiary of China to Ethiopia. 展开更多
关键词 AUTO ordinary tale
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Subverting the Traditional Function of Fairy Tales
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作者 李冬艳 《海外英语》 2012年第10X期191-192,共2页
Through analyzing Carter' s The Bloody Chamber and Other Stories,it is meant to expose the fact that the traditional function of fairy tales,namely the vehicle to enslave and indoctrinate children,has been subvert... Through analyzing Carter' s The Bloody Chamber and Other Stories,it is meant to expose the fact that the traditional function of fairy tales,namely the vehicle to enslave and indoctrinate children,has been subverted as a " loudspeaker" for long restrained,voiceless fe males to speak out their rights and identities,so as to dismantle the deeply-rooted patriarchy. 展开更多
关键词 FAIRY tale FUNCTION SUBVERSION
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TALEs:识别DNA的有力工具 被引量:2
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作者 曲晓辰 高志贤 +1 位作者 宁保安 刘颖 《解放军预防医学杂志》 CAS 2016年第2期277-280,共4页
植物致病菌黄单胞杆菌可以产生一种通过一连串的重复单元特异识别并激活植物基因启动子的蛋白—TALEs。TALEs与DNA识别的关键在于2个连续的多态性氨基酸可以组成1个TALE-DNA结合密码,每个密码特异结合1个核苷酸。整个TALEs蛋白与双链DN... 植物致病菌黄单胞杆菌可以产生一种通过一连串的重复单元特异识别并激活植物基因启动子的蛋白—TALEs。TALEs与DNA识别的关键在于2个连续的多态性氨基酸可以组成1个TALE-DNA结合密码,每个密码特异结合1个核苷酸。整个TALEs蛋白与双链DNA结合,其重复单元的顺序决定了可以结合的核苷酸序列。用人工设计的TALE核酸酶(TALENs)可以对包括植物、昆虫和哺乳动物在内的广泛的物种进行目的基因的编辑;而且人工设计的TALEs也可以与不同的功能结构融合,起到包括激活和调节基因表达在内的广泛作用,因此为生物技术和基因治疗方面提供了一个良好的工具。 展开更多
关键词 tales taleN DNA编辑
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千穗谷TALE转录因子家族的生物信息学分析 被引量:3
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作者 周敏 周涛 +3 位作者 徐倩 卢蕊 刘明稀 胡龙兴 《草地学报》 CAS CSCD 北大核心 2023年第3期676-687,共12页
TALE转录因子广泛存在于植物中,对植物的生长发育和对外界环境的响应发挥着重要作用。本研究在千穗谷(Amaranthus hypochondriacus)基因组中共鉴定出11个TALE基因。系统发育分析将千穗谷TALE家族蛋白分为BELL和KNOX两个亚家族,同一亚家... TALE转录因子广泛存在于植物中,对植物的生长发育和对外界环境的响应发挥着重要作用。本研究在千穗谷(Amaranthus hypochondriacus)基因组中共鉴定出11个TALE基因。系统发育分析将千穗谷TALE家族蛋白分为BELL和KNOX两个亚家族,同一亚家族中的AhTALEs基因具有相似的基因结构,编码蛋白也具有类似的结构域。蛋白二级结构和三级结构分析都表明AhTALEs蛋白由螺旋-角-螺旋的结构构成。此外,对千穗谷与其他物种的TALE基因共线性关系、组织表达特性和AhTALEs基因启动子区的顺式作用元件分析发现,不同AhTALEs亚家族在各组织中的表达模式不同,并且发现启动子区包含大量非生物胁迫和激素响应元件,这为AhTALE家族的进化分析提供了依据。qRT-PCR分析发现AhTALEs基因在低温和干旱胁迫下的表达水平有不同程度的变化,表明AhTALEs基因能够响应低温和干旱胁迫应答。本研究为开展千穗谷中TALE转录因子在非生物胁迫中的生物学功能研究提供了重要参考。 展开更多
关键词 千穗谷 tale 基因家族 低温 干旱
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应用“酶切连接”克隆法构建TALEs 被引量:1
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作者 梁龙 杨华 +3 位作者 杨永林 石国庆 刘守仁 皮文辉 《石河子大学学报(自然科学版)》 CAS 2013年第3期306-312,共7页
TALEs是在植物病原菌黄单胞杆菌(Xanthomonas)上发现的一类天然的DNA结合蛋白,其重复域已经成为模块化构建单元,用于人工DNA结合蛋白的构建。人工TALEs能够用于多种基因工程操作,包括转录调节和基因组编辑。为了在常规分子生物学实验室... TALEs是在植物病原菌黄单胞杆菌(Xanthomonas)上发现的一类天然的DNA结合蛋白,其重复域已经成为模块化构建单元,用于人工DNA结合蛋白的构建。人工TALEs能够用于多种基因工程操作,包括转录调节和基因组编辑。为了在常规分子生物学实验室稳定、经济地构建TALE-TFs和TALENs,本文采用TALE Toolbox试剂盒,运用PCR扩增和"酶切-连接"克隆法构建人工TALEs。结果表明,该构建策略是一种快速高效构建TALEs的方法,能够在1周内完成TALEs构建。 展开更多
关键词 转录激活因子样效应子 “酶切-连接”克隆法
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Action modes of transcription activator-like effectors(TALEs) of Xanthomonas in plants 被引量:6
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作者 XU Zheng-yin ZOU Li-fang +3 位作者 MA Wen-xiu CAI Lu-lu YANG Yang-yang CHEN Gong-you 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第12期2736-2745,共10页
Plant-pathogenic Xanthomonas infects a wide variety of host plants and causes many devastating diseases on crops. Transcription activator-like effectors(TALEs) are delivered by a type III secretion system(T3 SS) o... Plant-pathogenic Xanthomonas infects a wide variety of host plants and causes many devastating diseases on crops. Transcription activator-like effectors(TALEs) are delivered by a type III secretion system(T3 SS) of Xanthomonas into plant nuclei to directly bind specific DNA sequences(TAL effector-binding elements, EBEs) on either strand of host target genes with an unique modular DNA-binding domain and to bidirectionally drive host gene transcription. The target genes in plants consist of host susceptibility(S) genes promoting disease(ETS) and resistance(R) genes triggering defense(ETI). Here we generally summarized the discovery of TALEs in Xanthomonas species, their functions in bacterial pathogenicity in plants and their target genes in different host plants, and then focused on the newly revealed modes of protein action in triggering or suppressing plant defense. 展开更多
关键词 XANTHOMONAS tale itale ETS ETI SUPPRESSOR
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ZFNs和TALEs在艾滋病基因治疗中的价值
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作者 王伟大 武怡鸥 +2 位作者 张佳 廖端芳 李凯 《中南医学科学杂志》 CAS 2011年第4期460-462,共3页
随着对HIV病毒研究的深入以及传统治疗手段的局限性,通过基因治疗达到控制甚至治愈艾滋病受到重视。CCR5(趋化因子受体5)作为HIV-1进入人类白细胞的主要受体蛋白之一,其突变型CCR5Δ32携带者表现出一定抗HIV-1感染能力。本文综述两类基... 随着对HIV病毒研究的深入以及传统治疗手段的局限性,通过基因治疗达到控制甚至治愈艾滋病受到重视。CCR5(趋化因子受体5)作为HIV-1进入人类白细胞的主要受体蛋白之一,其突变型CCR5Δ32携带者表现出一定抗HIV-1感染能力。本文综述两类基因工程酶ZFNs和TALEs在艾滋病基因治疗中的应用价值。 展开更多
关键词 艾滋病 CCR5 ZFNs tales 基因治疗
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基于类转录激活因子效应物(TALEs)的基因组定点操控技术 被引量:2
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作者 赵美威 段承俐 刘江 《Zoological Research》 CAS CSCD 北大核心 2013年第5期509-518,共10页
基于类转录激活因子效应物(transcription activator-like effectors,TALEs)的基因组定点操控技术能够对基因组功能体系的反向遗传操作于基因及转录水平进行精确操控。TALEs是来源于植物病原菌—黄单胞杆菌(Xanthomonas spp.)的DNA结合... 基于类转录激活因子效应物(transcription activator-like effectors,TALEs)的基因组定点操控技术能够对基因组功能体系的反向遗传操作于基因及转录水平进行精确操控。TALEs是来源于植物病原菌—黄单胞杆菌(Xanthomonas spp.)的DNA结合蛋白,其结合域通常由包含34个氨基酸残基的重复模块串联而成。根据编码规则,可人为重新编排重复模块顺序使其能够识别新的DNA序列。该人工设计TALEs对基因组的定点操控(包括转录调控和基因组编辑)已在体外培养的人类细胞和多种模式生物中得到了成功应用,为模式生物基因功能研究,农作物性状改善及人类遗传性疾病治疗等开辟了新时代。 展开更多
关键词 类转录激活因子效应物 转录调控
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回归桃源 万物共生——艺拓国际神话言TALES2013新品赏析
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作者 王卫国 王周 《家具与室内装饰》 2012年第12期52-57,共6页
源自台湾的艺拓国际神话言TALES,以其东方时尚家具品牌的清新雅致形象,再度在2012年香港家庭用品展的"精萃廊"里惊艳亮相,成为"精萃廊"这一设计精品杰作汇聚所在,乃至整个展会的聚焦点。人们注意到,艺拓国际对于中... 源自台湾的艺拓国际神话言TALES,以其东方时尚家具品牌的清新雅致形象,再度在2012年香港家庭用品展的"精萃廊"里惊艳亮相,成为"精萃廊"这一设计精品杰作汇聚所在,乃至整个展会的聚焦点。人们注意到,艺拓国际对于中国传统文化和东方艺术的执著追求,生动地体现在他们每年一度在"精萃廊"里演绎的视觉艺术大餐;在文化创意上巧夺天工,在传统文化与艺术时尚之间交融互动的不懈努力,渗透到了产品本身及其展示呈现的每个细节里。 展开更多
关键词 艺拓国际 神话言tales 家具品牌
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On Folk Culture Translation in Tales of Hulan River from the Perspective of Deconstructionism 被引量:1
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作者 邓玉华 但雅琼 《海外英语》 2018年第20期175-177,共3页
Since the beginning of 21 th century, promoting culture export and spreading Chinese culture have become the top priorityof China. As the main carrier of culture, excellent literary works become the best choice for Ch... Since the beginning of 21 th century, promoting culture export and spreading Chinese culture have become the top priorityof China. As the main carrier of culture, excellent literary works become the best choice for Chinese culture"going out". The trans-lation of folk culture in Tale of Hulan River translated by Howard Goldblatt is analyzed from the perspective of deconstructionismwith an aim to give other translators some inspiration and enlightenment on translation of Chinese culture. 展开更多
关键词 Howard Goldblatt tales of Hulan River Folk Culture I106 A 1009-5039(201
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从“意境”到“译境”:基于翻译美学视域下的中译英歌词研究——以“Fairy Tale”为例
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作者 林煜聪 卢汉阳 《牡丹江教育学院学报》 2023年第12期36-39,共4页
随着文化全球化的深入发展,中译英歌词翻译已经成为跨文化交流的一个重要领域。歌词翻译中如何协调“意境”(原歌曲传达的情感与美感)与“译境”(源语言与目标语言之间的差异)的平衡,依然是一个充满挑战与争议的问题。文章回顾了翻译美... 随着文化全球化的深入发展,中译英歌词翻译已经成为跨文化交流的一个重要领域。歌词翻译中如何协调“意境”(原歌曲传达的情感与美感)与“译境”(源语言与目标语言之间的差异)的平衡,依然是一个充满挑战与争议的问题。文章回顾了翻译美学的发展,强调其在跨文化交流中的作用,通过分析“Fairy Tale”的翻译实践,展示了如何在不同的美学层面上实现文化适应和情感表达。研究结果强调了翻译作为一种文化表达方式的重要性,提出将翻译美学应用于实际翻译工作中,以促进跨文化交流和理解。 展开更多
关键词 意境 译境 翻译美学 翻译艺术 “Fairy tale
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Transplantation and Borrowing: the Spread of Northwest China's Folk Tales in East Asia
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作者 Su Yongqian 《Journal of Literature and Art Studies》 2019年第6期629-639,共11页
The spread of northwest China’s folk tales in the Donggan region of central Asia mainly includes two aspects: First, Donggan folk tales transplanted to the northwest folk tales. As the Donggan people migrated from no... The spread of northwest China’s folk tales in the Donggan region of central Asia mainly includes two aspects: First, Donggan folk tales transplanted to the northwest folk tales. As the Donggan people migrated from northwest China, folk tales from northwest China were also transplanted to central Asia. In the absence of written records, these stories also mutate as they are passed down through the mouths and ears of foreign peoples. Secondly, Donggan folk tales borrowed from northwest folk tales. Some plot units in Donggan folktales are very similar to those in northwest China, but the specific characters and plot structure of the whole story are obviously different from those in northwest China. This kind of folk stories are new stories created by Donggan people who borrowed the parent story of folk stories in northwest China and recombined it with other plots. With comparative study of folk tales, the profound cultural ties between the two places can be revealed. 展开更多
关键词 Central Asia Donggan China NORTHWEST FOLK tales
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The Tragedy of Man and Salvation Through Art:Marguerite Yourcenar’s Reflections on Human Condition in Oriental Tales
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作者 SONG Xin-yi 《Cultural and Religious Studies》 2022年第10期578-584,共7页
Written between 1936 and 1938,then continuously reworked in the following decades,Oriental Tales appeared for the first time byÉdition Gallimard in 1938 and reappeared in 1963 in a revised version.This work cover... Written between 1936 and 1938,then continuously reworked in the following decades,Oriental Tales appeared for the first time byÉdition Gallimard in 1938 and reappeared in 1963 in a revised version.This work covers most of the ancient cultures:Greek fables,Balkan ballads,Hindu or Chinese apologues,and Japanese medieval novels.This collection of tales has a so eccentric,exotic,distant,and archaic color that the author Marguerite Yourcenar did not hesitate to name it“Oriental”.The temporal and geographical remoteness of the stories reveals no less the depth of Yourcenar’s critical considerations on the human condition in general or in concrete terms.Man,entangled in the labyrinth of the world,is doomed to tragedy.Art,such as the writing that is in the making,presents a salutary way out. 展开更多
关键词 TRAGEDY SALVATION Yourcenar ART Oriental tales
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Edgar Allan Poe and His Horror Tales
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作者 李子月 《海外英语》 2012年第23期193-194,共2页
Edgar Allan Poe's life is a misery and the horror tales written by him are impressive,which has a strong sense of Gothic feeling.Like author,like his works.Almost every feature of Poe's horror tales could find... Edgar Allan Poe's life is a misery and the horror tales written by him are impressive,which has a strong sense of Gothic feeling.Like author,like his works.Almost every feature of Poe's horror tales could find explanation in his own life. 展开更多
关键词 HORROR tales GOTHIC FEATURES
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A New World to Discover——Grimm's Fairy Tales
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作者 莫槟菱 《海外英语》 2014年第15期182-183,共2页
Nowadays, the fairy tales have been on hot discussion. Some say that the very original Grimm's fairy tales is capricious that we may be more cautious whether it's suitable for children. With the rethinking of ... Nowadays, the fairy tales have been on hot discussion. Some say that the very original Grimm's fairy tales is capricious that we may be more cautious whether it's suitable for children. With the rethinking of fairy tales, many other scholars argue that the fairy tales can be valuable with its hidden German customs in the original version and the current versions are acceptable to children.The hot discussion raises our thinking. This new discussing wave may indicate something at a degree that the Grimm's Fairy Tales is worthy going deeper to study. We think that the Grimm's ferry tales is valuable for its pure essence and the German culture it hides that even adults should read it. Children can read the current version of Grimm's Fairy Tales with their innocence,getting the basic judging system of good and bad. The core question is that Adults also should pick it up. That's because usually people will find that all the experiences and reasons can be dated back to the simple truths in fairy tales. Meantime, the Grimm's Fairy Tales reflects lots of unthinkable customs and culture. The only thing we need to do is try to find new aspects and directions to explore the classic. With the spiritual and academic help, shouldn't the Grimm's fairy tales be necessary for both children and adults to read? 展开更多
关键词 FAIRY tales German CULTURES LINGUIST BROTHER Grimm
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"Historical Rewriting" and "Alternative Fairy Tales" in Contemporary Vietnamese Prose: Intertextuality or Incredulity Towards Grand Narratives
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作者 Hoang Cam Giang 《Cultural and Religious Studies》 2016年第6期346-354,共9页
At the end of the 20th century and beginning of the 21st century, Vietnamese literature witnessed unprecedented innovations in the aesthetic thinking of writers and the narrative structure of their works. One of the m... At the end of the 20th century and beginning of the 21st century, Vietnamese literature witnessed unprecedented innovations in the aesthetic thinking of writers and the narrative structure of their works. One of the most interesting manifestations of this new movement was not merely the introduction of new aesthetic materials and factors, but the adoption and positive "reprocessing" of traditional factors---especially historical factors and folk narrative factors. Based on intertextual theory, we would like to address the strong penetration, reproduction, and transformation of some folk storytelling models and historical materials in Vietnamese literature since 1986. Two corresponding phenomena we examine are "historical rewriting" and "alternative fairy tales"--as part of the whole process of incredulity towards grand narratives and postmodernism in the contemporary Vietnamese prose. 展开更多
关键词 INTERTEXTUALITY "alternative fairy tales "historical rewriting" contemporary prose grand narratives
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On Culture-Bound Words in the Brothers' Grimm Fairy Tales and Their Equivalents in English Translations
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作者 Hans-Harry Drofgiger 《Sociology Study》 2013年第12期962-973,共12页
The aim of the article is to connect the model of cognitive metonymy with the model of types of denotative equivalence highlighting the explanatory potential of the model of cognitive metonymy for describing and clari... The aim of the article is to connect the model of cognitive metonymy with the model of types of denotative equivalence highlighting the explanatory potential of the model of cognitive metonymy for describing and clarifying the use and occurrence of word equivalents in the English translations of the Brothers' Grimm fairy tales by Margaret Taylor (1914) and Jack Zipes (1987). The Fairy Tales of the Brothers Grimm (GFT) can be seen in a wide sense as a source of the history of culture and social life in the German speaking countries of the nineteenth century. Culture-bound words refer to a special cultural knowledge, which is in a historical sense not or not completely compatible with the structure of knowledge in our present days, and refer to a common cultural knowledge of the community of German speaking countries as well. This article describes the ways of using English equivalents in the translations of the GFT including lexical and cognitive procedures, which stand behind the use of certain equivalents. This leads to the theoretical question: Is it possible to extend the model of denotative equivalence using features of conceptual metonymy in rendering culture-bound words in the target texts? Investigating this, there can be established modifications in the theory of conceptual metonymy in the framework of cognitive linguistics. 展开更多
关键词 Fairy tale culture-bound words equivalence German language English language
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Develop the Adult Value in Andersen's Fairy Tales from Humanitarianism
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作者 田舒尧 《海外英语》 2017年第3期177-178,共2页
The Andersen’s fairy tales have made many research results since 20thcentury,but they mainly concentrate on its effect on children.Andersen’s Fairy Tales characterized by their"childishness",are unconsciou... The Andersen’s fairy tales have made many research results since 20thcentury,but they mainly concentrate on its effect on children.Andersen’s Fairy Tales characterized by their"childishness",are unconsciously referred to as"kids reading".People don’t understand the real meaning of Andersen’s fairy tales.This paper attempts to regard Anderson’s Fairy Tales as a great literary work but not only a fairy tales for children to read.And do a detailed research about"the adult value"in Andersen’s fairy tales from humanitarianism in the book.The whole paper is divided into three parts.Apart from the introduction,the paper starts with a series of specific examples and analysis about humanitarianism in the book.Upon that,it carries on analysis of adult value in Andersen’s Fairy Tales based on some serious problems in nowadays society.It seeks to expound the quality and contemporary value of Andersen’s fairy tales.And it also attempts to put it into good use for guiding the adults and help to solve social problems to some degree. 展开更多
关键词 andersen’s fairy tales HUMANITARIANISM adult value
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