Rational application of nitrogen （N） fertilizers is an important measure to raise N fertilizer recovery rate and reduce N loss.A two-year field experiment of rice-wheat rotation was employed to study the effects of ...Rational application of nitrogen （N） fertilizers is an important measure to raise N fertilizer recovery rate and reduce N loss.A two-year field experiment of rice-wheat rotation was employed to study the effects of N fertilization modes including a N fertilizer reduction and an organic manure replacement on crop yield,nutrient uptake,soil enzyme activity,and number of microbes as well as diversity of microbes.The result showed that 20% reduction of traditional N fertilizer dose of local farmers did not significantly change crop yield,N uptake,soil enzyme activity,and the number of microbes （bacteria,actinomycetes,and fungi）.On the basis of 20% reduction of N fertilizer,50% replacement of N fertilizer by organic manure increased the activity of sucrose,protease,urease,and phosphatase by 46-62,27-89,33-46,and 35-74%,respectively,and the number of microbes,i.e.,bacteria,actinomycetes,and fungi by 36-150,11-153,and 43-56%,respectively.Further,organic fertilizer replacement had a Shannon＇s diversity index （H） of 2.18,which was higher than that of other modes of single N fertilizer application.The results suggested that reducing N fertilizer by 20% and applying organic manure in the experimental areas could effectively lower the production costs and significantly improve soil fertility and biological properties.展开更多
A 15-year field experiment was carried out in Henan Province, China, to study the effects of different fertilization practices on yield of a wheat-maize rotation. Fertilizers tested contained N alone (N), N plus P (NP...A 15-year field experiment was carried out in Henan Province, China, to study the effects of different fertilization practices on yield of a wheat-maize rotation. Fertilizers tested contained N alone (N), N plus P (NP) or plus P and K (NPK), all with or without manure (M). Different long-term fertilization practices affected the yields under the rotation system of wheat and maize differently and the effects on yields was in a general trend of MNPK＞MNP＞MN＞NPK＞NP＞M＞N＞the control. The average contribution rate of soil fertility to the highest yield was 37.9%, and the rest 62.1% came from fertilizer applications. The yield effects of the chemical fertilizers were in the order of N＞P＞K and were increased by application of manure.Balanced fertilization with multielement chemical fertilizers and manure can be effective in maintaining growth in agricultural production. Combined application of chemical fertilizer and organic manure also increased the content of soil organic matter.展开更多
Soil fertility depletion is among the major impediments to sustained agricultural productivity especially in the less developed countries because of limited application of fertilizers. Soil fertility maintenance requi...Soil fertility depletion is among the major impediments to sustained agricultural productivity especially in the less developed countries because of limited application of fertilizers. Soil fertility maintenance requires a balanced application of inorganic and organic nutrient sources. This study was conducted on a Vertisol in Ethiopia to determine the optimum farm yard manure （M） and nitrogen （N） application rates for maximum return under cereal-pulse-cereal rotation system. The main and interaction effects of M and N significantly affected biomass, grain and straw yields of wheat （Triticum durum） and tef （Eragrostis tear）, but the residual effect on chickpea （Cicer arietinum） was not significant. Application of 6 t M ha^-1 and 30 kg N ha^-1, gave the largest grain yield of both crops but a comparable result was obtained due to 3 t M ha^-1 and 30 kg N ha^-1. The economic analysis revealed that 6.85 t M ha^-1 and 44 kg N ha^-1 for wheat, and 4.53 t M ha^-1 and 37 kg N ha^-1 for tef were the economic optimum rates. The additional benefit obtained due to these rates was about 450 USD ha^-1. Therefore, application of the economic optimum combination of both organic and inorganic sources of nitrogen is recommended for use on cereals in the cereal-legume-cereal rotation system.展开更多
Long-term application of chemical fertilizers causes soil degradation and nitrogen(N)loss,but these effects could be alleviated by organic fertilizers.In addition,crop rotation is a feasible practice to increase soil ...Long-term application of chemical fertilizers causes soil degradation and nitrogen(N)loss,but these effects could be alleviated by organic fertilizers.In addition,crop rotation is a feasible practice to increase soil fertility,soil quality and crop yields comparing with monocultural cropping patterns.However,questions remain concerning how the soil microbiome responds to different manure application rates under crop rotations.Here,we collected soil samples from a rice-rape system to investigate the response of the soil microbiome to nine years of pig manure application at different rates(CK:0 kg ha^(-1),M1:1930 kg ha^(-1),M2:3860 kg ha^(-1) and M3:5790 kg ha^(-1)).Our results revealed that the bacterialα-diversity(Chao1 and Shannon index)in the rape season increased first and then decreased with increasing manure application rates,and a high manure load tended to decrease the bacterialα-diversity in the rice season.Long-term manure application enriched some copiotrophic bacteria,such as Proteobacteria and Actinobacteria,while it decreased the relative abundance of Nitrospirae.Redundancy analysis(RDA)and the Mantel test indicated that soil pH,TC,TN,AP,C/P and N/P ratios were the main factors influencing bacterial communities.Moreover,network analysis showed that a low manure application rate shaped a complexly connected and stable bacterial community,while higher manure application rate decreased the stability of the bacterial network.These findings improve our understanding of bacterial responses to longterm manure application under crop rotations and their relationships with soil factors,especially in the context of increasing fertilizer inputs.展开更多
Green manure–crop rotation is a sustainable approach to protect crops against diseases and improve yield.However,the mechanism by which green manuring manipulates the crop-associated microbial community remains to be...Green manure–crop rotation is a sustainable approach to protect crops against diseases and improve yield.However,the mechanism by which green manuring manipulates the crop-associated microbial community remains to be elucidated.In this study,we explored the horizontal processes of bacterial communities in different compartments of the soil–root interface(bulk soil,rhizosphere soil,rhizoplane and endosphere)of tobacco by performing a field experiment including four rotation practices,namely,tobacco rotated with smooth vetch,ryegrass,radish,and winter fallow(without green manure).Results showed that the co-occurrence networks constructed by adjacent compartments of the soil–root interface with green manuring had more edges than without green manuring,indicating dramatic microbial interactions.Green manuring increased the dispersal-niche continuum index between bulk soil and other compartments,indicating that it facilitated the horizontal dispersal of microbes.For the different green manuring practices,the neutral community model explained 24.6–27.6%of detection frequency for bacteria,and at least one compartment under each practice had a normalized stochasticity ratio higher than the 50%boundary point,suggesting that the deterministic and stochastic processes jointly shaped the tobacco microbiome.In conclusion,green manuring generally facilitates bacterial community dispersal across different compartments and enhances potential interactions among adjacent compartments.This study provides empirical evidence for understanding the microbiome assembly under green manure–crop rotation.展开更多
小麦与豆科绿肥轮作和合理施用氮肥对黄土高原旱地缺素地区具有特别重要意义。本试验以夏休闲为对照,比较了小麦与绿豆、大豆、秋豆轮作和氮肥水平（0、108、1351、62 kg/hm2）对小麦产量和籽粒养分的影响。结果表明,小麦与豆科绿肥轮作...小麦与豆科绿肥轮作和合理施用氮肥对黄土高原旱地缺素地区具有特别重要意义。本试验以夏休闲为对照,比较了小麦与绿豆、大豆、秋豆轮作和氮肥水平（0、108、1351、62 kg/hm2）对小麦产量和籽粒养分的影响。结果表明,小麦与豆科绿肥轮作,在黄土高原旱地受水分限制影响,小麦产量降低9.7%~26.6%;同时,与休闲处理相较,小麦籽粒养分携出量显著降低或表现出降低的趋势,其中,与绿豆轮作,小麦籽粒养分携出量降低最多,为10.5%~29.3%,大豆次之,为3.1%~12.8%,与当地大豆品种秋豆轮作,小麦籽粒养分携出量降低最少,为0.98%~11.7%。然而,小麦籽粒营养元素锌、磷、钾、钙、镁的含量与休闲处理相比,分别增加22.0%、18.5%、8.8%、7.9%、7.8%。小麦与绿肥轮作后,108 kg N/hm2（当地常规施氮量的80%）获得了较高小麦生物量和产量。同时,随施氮量的增加,小麦籽粒氮、硫、钙、铁、铜、锌的含量和养分携出量均表现出增加趋势;小麦籽粒含磷量和养分携出量有下降趋势;小麦籽粒锰含量呈现降低趋势但养分携出量增加;钾、镁的含量和养分携出量随施氮量无显著变化。展开更多
基金supported by the National Basic Research Program of China (973 Program,2007CB109308)the National High-Tech R&D Program of China (2007AA06Z332)a special grant of Application of Nuclear Techniques in Agriculture from the Ministry of Agriculture of China (200803034)
文摘Rational application of nitrogen （N） fertilizers is an important measure to raise N fertilizer recovery rate and reduce N loss.A two-year field experiment of rice-wheat rotation was employed to study the effects of N fertilization modes including a N fertilizer reduction and an organic manure replacement on crop yield,nutrient uptake,soil enzyme activity,and number of microbes as well as diversity of microbes.The result showed that 20% reduction of traditional N fertilizer dose of local farmers did not significantly change crop yield,N uptake,soil enzyme activity,and the number of microbes （bacteria,actinomycetes,and fungi）.On the basis of 20% reduction of N fertilizer,50% replacement of N fertilizer by organic manure increased the activity of sucrose,protease,urease,and phosphatase by 46-62,27-89,33-46,and 35-74%,respectively,and the number of microbes,i.e.,bacteria,actinomycetes,and fungi by 36-150,11-153,and 43-56%,respectively.Further,organic fertilizer replacement had a Shannon＇s diversity index （H） of 2.18,which was higher than that of other modes of single N fertilizer application.The results suggested that reducing N fertilizer by 20% and applying organic manure in the experimental areas could effectively lower the production costs and significantly improve soil fertility and biological properties.
文摘A 15-year field experiment was carried out in Henan Province, China, to study the effects of different fertilization practices on yield of a wheat-maize rotation. Fertilizers tested contained N alone (N), N plus P (NP) or plus P and K (NPK), all with or without manure (M). Different long-term fertilization practices affected the yields under the rotation system of wheat and maize differently and the effects on yields was in a general trend of MNPK＞MNP＞MN＞NPK＞NP＞M＞N＞the control. The average contribution rate of soil fertility to the highest yield was 37.9%, and the rest 62.1% came from fertilizer applications. The yield effects of the chemical fertilizers were in the order of N＞P＞K and were increased by application of manure.Balanced fertilization with multielement chemical fertilizers and manure can be effective in maintaining growth in agricultural production. Combined application of chemical fertilizer and organic manure also increased the content of soil organic matter.
基金sponsored by the Debre Zeit Centre of the Ethiopian Agricultural Research Organization (EARO).
文摘Soil fertility depletion is among the major impediments to sustained agricultural productivity especially in the less developed countries because of limited application of fertilizers. Soil fertility maintenance requires a balanced application of inorganic and organic nutrient sources. This study was conducted on a Vertisol in Ethiopia to determine the optimum farm yard manure （M） and nitrogen （N） application rates for maximum return under cereal-pulse-cereal rotation system. The main and interaction effects of M and N significantly affected biomass, grain and straw yields of wheat （Triticum durum） and tef （Eragrostis tear）, but the residual effect on chickpea （Cicer arietinum） was not significant. Application of 6 t M ha^-1 and 30 kg N ha^-1, gave the largest grain yield of both crops but a comparable result was obtained due to 3 t M ha^-1 and 30 kg N ha^-1. The economic analysis revealed that 6.85 t M ha^-1 and 44 kg N ha^-1 for wheat, and 4.53 t M ha^-1 and 37 kg N ha^-1 for tef were the economic optimum rates. The additional benefit obtained due to these rates was about 450 USD ha^-1. Therefore, application of the economic optimum combination of both organic and inorganic sources of nitrogen is recommended for use on cereals in the cereal-legume-cereal rotation system.
基金supported by the National Key Research and Development Program of China(2017YFD0800404)the National Natural Science Foundation of China(41671249)Fundamental Research Funds for the Central Universities(2019QNA6011).
文摘Long-term application of chemical fertilizers causes soil degradation and nitrogen(N)loss,but these effects could be alleviated by organic fertilizers.In addition,crop rotation is a feasible practice to increase soil fertility,soil quality and crop yields comparing with monocultural cropping patterns.However,questions remain concerning how the soil microbiome responds to different manure application rates under crop rotations.Here,we collected soil samples from a rice-rape system to investigate the response of the soil microbiome to nine years of pig manure application at different rates(CK:0 kg ha^(-1),M1:1930 kg ha^(-1),M2:3860 kg ha^(-1) and M3:5790 kg ha^(-1)).Our results revealed that the bacterialα-diversity(Chao1 and Shannon index)in the rape season increased first and then decreased with increasing manure application rates,and a high manure load tended to decrease the bacterialα-diversity in the rice season.Long-term manure application enriched some copiotrophic bacteria,such as Proteobacteria and Actinobacteria,while it decreased the relative abundance of Nitrospirae.Redundancy analysis(RDA)and the Mantel test indicated that soil pH,TC,TN,AP,C/P and N/P ratios were the main factors influencing bacterial communities.Moreover,network analysis showed that a low manure application rate shaped a complexly connected and stable bacterial community,while higher manure application rate decreased the stability of the bacterial network.These findings improve our understanding of bacterial responses to longterm manure application under crop rotations and their relationships with soil factors,especially in the context of increasing fertilizer inputs.
基金supported financially by the National Key Research and Development Program of China(2021YFD1700200)the China Agriculture Research System of MOF and MARA(CARS-22).
文摘Green manure–crop rotation is a sustainable approach to protect crops against diseases and improve yield.However,the mechanism by which green manuring manipulates the crop-associated microbial community remains to be elucidated.In this study,we explored the horizontal processes of bacterial communities in different compartments of the soil–root interface(bulk soil,rhizosphere soil,rhizoplane and endosphere)of tobacco by performing a field experiment including four rotation practices,namely,tobacco rotated with smooth vetch,ryegrass,radish,and winter fallow(without green manure).Results showed that the co-occurrence networks constructed by adjacent compartments of the soil–root interface with green manuring had more edges than without green manuring,indicating dramatic microbial interactions.Green manuring increased the dispersal-niche continuum index between bulk soil and other compartments,indicating that it facilitated the horizontal dispersal of microbes.For the different green manuring practices,the neutral community model explained 24.6–27.6%of detection frequency for bacteria,and at least one compartment under each practice had a normalized stochasticity ratio higher than the 50%boundary point,suggesting that the deterministic and stochastic processes jointly shaped the tobacco microbiome.In conclusion,green manuring generally facilitates bacterial community dispersal across different compartments and enhances potential interactions among adjacent compartments.This study provides empirical evidence for understanding the microbiome assembly under green manure–crop rotation.
文摘小麦与豆科绿肥轮作和合理施用氮肥对黄土高原旱地缺素地区具有特别重要意义。本试验以夏休闲为对照,比较了小麦与绿豆、大豆、秋豆轮作和氮肥水平（0、108、1351、62 kg/hm2）对小麦产量和籽粒养分的影响。结果表明,小麦与豆科绿肥轮作,在黄土高原旱地受水分限制影响,小麦产量降低9.7%~26.6%;同时,与休闲处理相较,小麦籽粒养分携出量显著降低或表现出降低的趋势,其中,与绿豆轮作,小麦籽粒养分携出量降低最多,为10.5%~29.3%,大豆次之,为3.1%~12.8%,与当地大豆品种秋豆轮作,小麦籽粒养分携出量降低最少,为0.98%~11.7%。然而,小麦籽粒营养元素锌、磷、钾、钙、镁的含量与休闲处理相比,分别增加22.0%、18.5%、8.8%、7.9%、7.8%。小麦与绿肥轮作后,108 kg N/hm2（当地常规施氮量的80%）获得了较高小麦生物量和产量。同时,随施氮量的增加,小麦籽粒氮、硫、钙、铁、铜、锌的含量和养分携出量均表现出增加趋势;小麦籽粒含磷量和养分携出量有下降趋势;小麦籽粒锰含量呈现降低趋势但养分携出量增加;钾、镁的含量和养分携出量随施氮量无显著变化。
