The buckling behavior of single layer space structure is very sensitive. The joint rigidity, moreover, is one of the main factors of stability which may determine the entire failure behavior. Thus, the reasonable stif...The buckling behavior of single layer space structure is very sensitive. The joint rigidity, moreover, is one of the main factors of stability which may determine the entire failure behavior. Thus, the reasonable stiffness of joint system, which is neither total pin assumption nor perfect fix condition, is very important to apply to the real single layer space one. Therefore, the purpose of this work was to investigate the buckling behavior of single layer space structure, using the development of the upgraded stiffness matrix for the joint rigidity. To derive tangential stiffness matrix, a displacement function was assumed using translational and rotational displacement at the node. The geometrical nonlinear analysis was simulated not only with perfect model but also with imperfect one. As a result, the one and two free nodal numerical models were investigated using derived stiffness matrix. It was figured out that the buckling load increases in proportion to joint rigidity with rise-span ratio. The stability of numerical model is very sensitive with the initial imperfection, responding of bifurcation in the structure.展开更多
Objective Prader-Willi Sydrome （PWS） is a human disorder related to genomic imprinting defect on 15ql 1-13. It is characterized by a series of classic features such as hypotonia, hyperphagia, obesity, osteoporosis, ...Objective Prader-Willi Sydrome （PWS） is a human disorder related to genomic imprinting defect on 15ql 1-13. It is characterized by a series of classic features such as hypotonia, hyperphagia, obesity, osteoporosis, typical facial and body dysmorphosis, hypogonadism, mental and behaviour disorders. Our study was designed to precisely detect the microdeletions, which accounts for 65%-70% of the PWS. Methods Physical and laboratory examinations were firstly performed to diagnose PWS clinically, and to discover novel clinical features. Then the patient was screened with bisulfite-specific sequencing and precisely delineated through high-density array CGH. Results With the bisulfite-specific sequencing, the detected CpG island in the PWS critical region was found homozygously hypermethylated. Then with array CGH, a 2.22 Mb type II microdeletion was detected, covering a region from MKRN3, MAGEL2, NDN, PWRN2, PWRN1, Cl2orf2, SNURF-SNRPN, C/D snoRNAs, to distal of UBE3A. Conclusions Array CGH, after the fast screening of Bisulfite-specific sequencing, is a feasible and precise method to detect microdeletions in PWS patients. A novel feature of metacarpophalangeal joint rigidity was also presented, which is the first time reported in PWS.展开更多
基金Project(12 High-tech Urban C11) supported by High-tech Urban Development Program of Ministry of Land,Transport and Maritime Affairs,Korea
文摘The buckling behavior of single layer space structure is very sensitive. The joint rigidity, moreover, is one of the main factors of stability which may determine the entire failure behavior. Thus, the reasonable stiffness of joint system, which is neither total pin assumption nor perfect fix condition, is very important to apply to the real single layer space one. Therefore, the purpose of this work was to investigate the buckling behavior of single layer space structure, using the development of the upgraded stiffness matrix for the joint rigidity. To derive tangential stiffness matrix, a displacement function was assumed using translational and rotational displacement at the node. The geometrical nonlinear analysis was simulated not only with perfect model but also with imperfect one. As a result, the one and two free nodal numerical models were investigated using derived stiffness matrix. It was figured out that the buckling load increases in proportion to joint rigidity with rise-span ratio. The stability of numerical model is very sensitive with the initial imperfection, responding of bifurcation in the structure.
基金supported by grants from National 973 Program(2006CB503901)Shanghai Key Laboratory of Diabetes Mellitus(08DZ2230200)+1 种基金Major Program of Shanghai Municipality for Basic Research(08dj 1400601)Program for Outstanding Medical Academic Leader in Shanghai (LJ06010).
文摘Objective Prader-Willi Sydrome （PWS） is a human disorder related to genomic imprinting defect on 15ql 1-13. It is characterized by a series of classic features such as hypotonia, hyperphagia, obesity, osteoporosis, typical facial and body dysmorphosis, hypogonadism, mental and behaviour disorders. Our study was designed to precisely detect the microdeletions, which accounts for 65%-70% of the PWS. Methods Physical and laboratory examinations were firstly performed to diagnose PWS clinically, and to discover novel clinical features. Then the patient was screened with bisulfite-specific sequencing and precisely delineated through high-density array CGH. Results With the bisulfite-specific sequencing, the detected CpG island in the PWS critical region was found homozygously hypermethylated. Then with array CGH, a 2.22 Mb type II microdeletion was detected, covering a region from MKRN3, MAGEL2, NDN, PWRN2, PWRN1, Cl2orf2, SNURF-SNRPN, C/D snoRNAs, to distal of UBE3A. Conclusions Array CGH, after the fast screening of Bisulfite-specific sequencing, is a feasible and precise method to detect microdeletions in PWS patients. A novel feature of metacarpophalangeal joint rigidity was also presented, which is the first time reported in PWS.
