期刊文献+
共找到2,249篇文章
< 1 2 113 >
每页显示 20 50 100
土石坝护坡冻胀破坏水-热-力耦合数值模拟研究
1
作者 李卓 姜鑫 +3 位作者 张继勋 赵昱 王虞清 方艺翔 《水利水运工程学报》 CSCD 北大核心 2024年第3期136-145,共10页
针对寒区土石坝护坡普遍存在的冻胀破坏问题,选取寒区某土石坝工程为研究对象,结合土石坝工程特点,建立考虑水-热-力耦合作用的有限元计算模型。研究护坡在库水位和坝体填土水分迁移作用下冻胀破坏全过程,分析土石坝温度场、水分场和位... 针对寒区土石坝护坡普遍存在的冻胀破坏问题,选取寒区某土石坝工程为研究对象,结合土石坝工程特点,建立考虑水-热-力耦合作用的有限元计算模型。研究护坡在库水位和坝体填土水分迁移作用下冻胀破坏全过程,分析土石坝温度场、水分场和位移场的变化规律,并将位移场有限元计算结果与实测冻胀变形资料进行对比,验证有限元计算结果。研究结果表明:冻胀作用对坝坡影响深度约为2 m,坝坡浅层0~2 m范围的温度变化受外界气温影响较大,坝体内部温度相对坝坡浅层变幅小,且有一定的滞后性;负温使坝坡浅层孔隙水相变成冰,宏观上表现为未冻水体积分数的降低,坝坡浅层土石混合体孔隙水相变、坝体填土内水分向坝坡迁移和冻结峰面向坝体内部移动是护坡冻胀破坏的主要因素;大坝坝坡计算冻胀量为20~30 cm,最大值为36 cm,计算结果与实测冻胀变形资料吻合;护坡冻胀破坏主要由砂砾料垫层、坝体填土等土石混合体冻胀及冰推力共同作用引起。研究结果可为寒区土石坝护坡设计提供参考。 展开更多
关键词 土石坝 护坡 冻胀破坏 水-热-力耦合 寒区
下载PDF
基于时序InSAR的老挝桑片-桑南内水库大坝溃坝回溯研究
2
作者 徐云乾 袁明道 +3 位作者 史永胜 祝二浩 刘宜杰 张旭辉 《大坝与安全》 2024年第2期36-41,共6页
InSAR是一种用于水库大坝表面位移监测的新技术,具有周期性、低延迟的特点,且能回溯历史数据。利用老挝桑片-桑南内水库大坝溃坝前期及溃坝期间的卫星影像,收集溃坝前后的大坝表面位移监测数据和降雨数据,对大坝自身和管理方面的溃坝原... InSAR是一种用于水库大坝表面位移监测的新技术,具有周期性、低延迟的特点,且能回溯历史数据。利用老挝桑片-桑南内水库大坝溃坝前期及溃坝期间的卫星影像,收集溃坝前后的大坝表面位移监测数据和降雨数据,对大坝自身和管理方面的溃坝原因进行了分析,结果表明大坝溃坝前已发生较明显的异常变形趋势。本研究将InSAR技术引入大坝风险管理中,能有效减少溃坝事故的发生。 展开更多
关键词 土石坝 溃坝 INSAR
下载PDF
Seepage simulation of high concrete-faced rockfill dams based on generalized equivalent continuum model 被引量:6
3
作者 Shou-kai Chen Qi-dong He Ji-gang Cao 《Water Science and Engineering》 EI CAS CSCD 2018年第3期250-257,共8页
This research focused on the three-dimensional(3 D) seepage field simulation of a high concrete-faced rockfill dam(CFRD) under complex hydraulic conditions. A generalized equivalent continuum model of fractured rock m... This research focused on the three-dimensional(3 D) seepage field simulation of a high concrete-faced rockfill dam(CFRD) under complex hydraulic conditions. A generalized equivalent continuum model of fractured rock mass was used for equivalent continuous seepage field analysis based on the improved node virtual flow method. Using a high CFRD as an example, the generalized equivalent continuum range was determined, and a finite element model was established based on the terrain and geological conditions, as well as structural face characteristics of the dam area. The equivalent seepage coefficients of different material zones or positions in the dam foundation were calculated with the Snow model or inverse analysis. Then, the 3 D seepage field in the dam area was calculated under the normal water storage conditions, and the corresponding water head distribution, seepage flow, seepage gradient, and seepage characteristics in the dam area were analyzed. The results show that the generalized equivalent continuum model can effectively simulate overall seepage patterns of the CFRD under complex hydraulic conditions and provide a reference for seepage analysis of similar CFRDs. 展开更多
关键词 Concrete-faced ROCKFILL dam(CFRD) GENERALIZED equivalent CONTINUUM model Node virtual flow method Fractured rock mass SEEPAGE field SEEPAGE coefficient
下载PDF
Seepage safety monitoring model for an earth rock dam under influence of high-impact typhoons based on particle swarm optimization algorithm 被引量:6
4
作者 Yan Xiang Shu-yan Fu +2 位作者 Kai Zhu Hui Yuan Zhi-yuan Fang 《Water Science and Engineering》 EI CAS CSCD 2017年第1期70-77,共8页
Extreme hydrological events induced by typhoons in reservoir areas have presented severe challenges to the safe operation of hydraulic structures. Based on analysis of the seepage characteristics of an earth rock dam,... Extreme hydrological events induced by typhoons in reservoir areas have presented severe challenges to the safe operation of hydraulic structures. Based on analysis of the seepage characteristics of an earth rock dam, a novel seepage safety monitoring model was constructed in this study. The nonlinear influence processes of the antecedent reservoir water level and rainfall were assumed to follow normal distributions. The particle swarm optimization (PSO) algorithm was used to optimize the model parameters so as to raise the fitting accuracy. In addition, a mutation factor was introduced to simulate the sudden increase in the piezometric level induced by short-duration heavy rainfall and the possible historical extreme reservoir water level during a typhoon. In order to verify the efficacy of this model, the earth rock dam of the Siminghu Reservoir was used as an example. The piezometric level at the SW1-2 measuring point during Typhoon Fitow in 2013 was fitted with the present model, and a corresponding theoretical expression was established. Comparison of fitting results of the piezometric level obtained from the present statistical model and traditional statistical model with monitored values during the typhoon shows that the present model has a higher fitting accuracy and can simulate the uprush feature of the seepage pressure during the typhoon perfectly. 展开更多
关键词 Monitoring model Particle swarm optimization algorithm Earth rock dam Lagging effect TYPHOON Seepage pressure Mutation factor Piezometric level
下载PDF
Theory and Application of System Integration for Real-Time Monitoring of Core Rock-Fill Dam Filling Construction Quality 被引量:2
5
作者 崔博 钟登华 《Transactions of Tianjin University》 EI CAS 2012年第3期173-179,共7页
The theory and method of system integration for the real-time monitoring of core rock-fill dam filling con- struction quality are studied in this paper. First, the importance analysis of system integration factors is ... The theory and method of system integration for the real-time monitoring of core rock-fill dam filling con- struction quality are studied in this paper. First, the importance analysis of system integration factors is carried out with the analytic hierarchy process. Then, according to the analysis result of integration factors, the conceptual model of system integration is built based on function integration, index integration, technology integration and information integration, the index structure of core rock-fill dam filling construction quality control is constructed and the method of function integration and technology integration is studied. The mathematical model of process monitoring is built according to monitoring objective, process and indexes. Research results have been applied in Nuozhadu core rock-fill dam construction management, realizing system integration through building appropriate monitoring work flow and comprehensive information platform of digital dam. 展开更多
关键词 核心摇充满水坝 充满的构造质量 即时监视 系统集成 概念的模型 数学模型
下载PDF
Optimizing Earth Allocation for Rock-Fill Dam Construction 被引量:1
6
作者 钟登华 胡程顺 张静 《Transactions of Tianjin University》 EI CAS 2005年第2期146-151,共6页
An optimal allocation of earth is of great significance to reduce the project cost and duration in the construction of rock-fill dams. The earth allocation is a dynamic system affected by various time-space constraint... An optimal allocation of earth is of great significance to reduce the project cost and duration in the construction of rock-fill dams. The earth allocation is a dynamic system affected by various time-space constraints. Based on previous studies, a new method of optimizing this dynamic system as a static one is presented. In order to build a generalized and flexible model of the problem, some man-made constraints were investigated in building the mathematic model. Linear programming and simplex method are introduced to solve the optimization problem of earth allocation. A case study in a large-scale rock-fill dam construction project is presented to demonstrate the proposed method and its successful application shows the feasibility and effectiveness of the method. 展开更多
关键词 水坝 建筑物 动态分配 线性规划 结构特点 设计方案
下载PDF
基于PS-InSAR的小浪底土石坝形变监测与稳定性模拟 被引量:1
7
作者 刘辉 朱梦源 +2 位作者 赵文飞 李葛爽 周泊辰 《华北水利水电大学学报(自然科学版)》 北大核心 2024年第1期1-10,共10页
探索土石坝形变规律及稳定性分析是全球水利工程所关注的一个重大问题,对保障人民群众生命安全和经济健康发展具有重大意义。采用2017年3月至2021年9月小浪底土石坝55景Sentinel-1A卫星SAR影像,利用PS-InSAR技术,监测到小浪底土石坝近5... 探索土石坝形变规律及稳定性分析是全球水利工程所关注的一个重大问题,对保障人民群众生命安全和经济健康发展具有重大意义。采用2017年3月至2021年9月小浪底土石坝55景Sentinel-1A卫星SAR影像,利用PS-InSAR技术,监测到小浪底土石坝近5年从坝顶至坝底呈现“阶梯状”形变趋势,最大形变量达到-86.09 mm;并结合库水位、降雨量等因素,发现坝体整体呈现“夏急冬缓”的季节性形变趋势。进一步采用二维有限元方法,对小浪底土石坝的模拟模型进行渗流、应力分析。在高库水位情况下,相较于低水位情况,坝体渗流量更大、形变速率更大;在应力分析部分,发现坝体的垂直向形变趋势与InSAR监测结果相吻合,亦呈现“阶梯状”形变。研究成果不仅为小浪底土石坝运行稳定性分析及形变历史回溯提供理论基础和决策依据,也说明PS-InSAR技术与二维有限元模型模拟技术在水利工程监测方面具有巨大的应用潜力。 展开更多
关键词 PS-INSAR 土石坝 形变监测 稳定性分析 库水位
下载PDF
基于Vine-Copula的高土石坝变形监控模型研究
8
作者 陈天赐 李艳玲 +1 位作者 张芳 陈枭 《人民长江》 北大核心 2024年第5期206-212,218,共8页
针对高土石坝变形监控模型主要基于单一测点,无法定量考虑测点空间关联性的问题,构建了基于Vine-Copula的变形监控模型,并提出了基于蒙特卡洛随机抽样法的空间置信域预警阈值设置方法。在模型构建过程中,充分考虑了多测点之间的时序特... 针对高土石坝变形监控模型主要基于单一测点,无法定量考虑测点空间关联性的问题,构建了基于Vine-Copula的变形监控模型,并提出了基于蒙特卡洛随机抽样法的空间置信域预警阈值设置方法。在模型构建过程中,充分考虑了多测点之间的时序特性和空间相关性,利用Vine-Copula方法对变形数据进行精确建模和分析,以揭示高土石坝变形的整体趋势。同时,通过蒙特卡洛随机抽样法确定了空间置信域,为预警阈值的设定提供了科学依据。工程实践表明:该模型模拟结果能够准确反映高土石坝变形的整体趋势,具有较高的合理性和精度,有效实现了监测效应量向高土石坝空间全域的拓展。研究成果可为高土石坝安全监控提供新的思路和方法,具有重要的理论意义和实践价值。 展开更多
关键词 高土石坝 变形监控模型 Vine结构 COPULA函数 空间置信域
下载PDF
Evaluation of ability of Rock Check Dam to prevent the transportation of Pb-contaminated sediment in Khli Ti Creek, Thailand 被引量:1
9
作者 Paweena Panichayapichet Suwanchai Nitisoravut Arpa Wangkiat 《Chinese Journal Of Geochemistry》 EI CAS 2006年第B08期119-120,共2页
关键词 沉积物 环境污染 重金属 水体污染
下载PDF
The Deformation and Stress Analysis of Na Ba Reservoir Concrete Face Rock-Fill Dam
10
作者 Yin Shaofei Peng Hui +3 位作者 Wu Fan Wen Chen Jin Ke Han Kai 《Journal of Environmental Science and Engineering(B)》 2016年第10期489-494,共6页
关键词 混凝土面板堆石坝 应力分析 坝变形 水库 三维非线性有限元分析 ANSYS软件 NA 应力变形分析
下载PDF
Comparison between the Isotope Tracking Method and Resistivity Tomography of Earth Rock-Fill Dam Seepage Detection
11
作者 Mingjie Zhao Hui Wang +1 位作者 Xiao Sun Yao Zhang 《Engineering(科研)》 2011年第4期389-399,共11页
This paper compares the different inversion results of three different earth rock-fill dam models with the actual leakage passages by performing isotope tracing tests and resistivity tomographic tests. The accuracy of... This paper compares the different inversion results of three different earth rock-fill dam models with the actual leakage passages by performing isotope tracing tests and resistivity tomographic tests. The accuracy of the experimental results is evaluated, and the characteristics of these two methods are analyzed. As a result, some significant references are offered for earth rock-fill dam’s hidden defects detection. The experimental results show that the leakage and the direction of the seepage can be judged by isotope tracing tests, meanwhile, the degree of the leakage can be confirmed through the determination of the horizontal seepage velocity and the vertical seepage velocity, but it is difficult to properly determine the position of leakage passages and the range of leakage. Relatively speaking, the positions of the leakage passages can be accurately and directly displayed through resistivity tomographic tests. The experiment results show that the resistivity tomographic method is much better than isotope tracing method with regard to earth rock-fill dam’s hidden defects detection, and the resistivity tomographic method expresses much more convenience and much higher precision than isotope tracing method. 展开更多
关键词 EARTH Rock-Fill Dam LEAKAGE Diagnosis ISOTOPES TRACING RESISTIVITY Tomography
下载PDF
Application of 3D Electrical Resistivity Tomography for Diagnosing Leakage in Earth Rock-Fill Dam
12
作者 Xin Zhang Mingjie Zhao +2 位作者 Kui Wang Pan Liu Huan Liu 《Engineering(科研)》 2016年第5期269-275,共7页
The leakage occurs during operation of the dam in Liuhuanggou reservoir. It’s a threat to the safety of the people’s lives and property in downstream. In order to eliminate the hidden danger of reservoir, ensure the... The leakage occurs during operation of the dam in Liuhuanggou reservoir. It’s a threat to the safety of the people’s lives and property in downstream. In order to eliminate the hidden danger of reservoir, ensure the safety of the dam, play better the function of flood control and water storage of the reservoir etc., we apply the 3D electrical resistivity tomography detecting technology and volume rendering image processing technology, make the measurement in field, process the data and combine the field survey to find out the leakage channels inside the dam. The results show that the 3D resistivity images appear the low resistivity zone corresponding with the leakage channels. There are two main leakage channels that come from different location inside the dam. It is feasible to diagnose the leakage in earth rock-fill dam by applying 3D electrical resistivity tomography. 展开更多
关键词 Earth Rock-Fill Dam LEAKAGE 3D Electrical Resistivity Tomography Volume Rendering
下载PDF
L-3-正丁苯酞对实验性自身免疫性脑脊髓炎的作用及其机制
13
作者 韩红霞 张晋欣 《山西医科大学学报》 CAS 2024年第2期184-191,共8页
目的 基于RhoA/ROCK信号通路探讨L-3-正丁苯酞(L-3-n-butylphthalide, NBP)对实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis, EAE)小鼠的疗效及潜在机制。方法 32只雌性C57BL/6小鼠随机分为4组:对照组、EAE组、... 目的 基于RhoA/ROCK信号通路探讨L-3-正丁苯酞(L-3-n-butylphthalide, NBP)对实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis, EAE)小鼠的疗效及潜在机制。方法 32只雌性C57BL/6小鼠随机分为4组:对照组、EAE组、NBP低剂量组(L-NBP)和NBP高剂量组(H-NBP),每组8只。使用髓鞘少突胶质细胞糖蛋白(myelin oligodendrocyte glycoprotein 35-55, MOG35-55)作为抗原乳剂免疫诱导C57BL/6小鼠建立EAE模型。EAE模型制作成功后,L-NBP组和H-NBP组分别以3.25,6.5 mg/(kg·d)NBP腹腔注射,连续28 d。免疫当日起,每7 d记录各组小鼠的体质量,每天进行神经功能障碍评分;对脊髓组织进行HE染色和劳克坚牢蓝(Luxol fast blue, LFB)染色,观察病理改变;使用酶联免疫吸附试验法(ELISA)检测外周血白细胞介素-10(IL-10)、IL-1β、IL-6和IL-18含量;real time-PCR检测脊髓组织炎症相关因子肿瘤坏死因子(TNF)-α、TNF受体1(TNFR1)、IL-1β的表达;Western blot检测RhoA、ROCKⅠ及ROCKⅡ蛋白表达情况。结果 与对照组相比,EAE组小鼠体质量下降,神经功能障碍评分、组织病理学评分升高,IL-10含量降低,IL-18、IL-6和IL-1β含量增加,TNF-α、TNFR1及IL-1β mRNA表达升高,RhoA、ROCKⅠ及ROCKⅡ蛋白表达增高,差异均有统计学意义(P<0.05)。与EAE组相比,L-NBP和H-NBP组小鼠体质量增加,发病潜伏期延长,高峰期延迟,神经功能障碍评分、组织病理学评分降低,IL-10含量增高,IL-1β、IL-6和IL-18含量降低,TNF-α、TNFR1及IL-1β mRNA表达降低,RhoA、ROCKⅠ和ROCKⅡ蛋白表达降低,差异均有统计学意义(P<0.05)。与L-NBP组相比,H-NBP组小鼠体质量增加,发病潜伏期延长,高峰期延迟,神经功能障碍评分、组织病理学评分降低,IL-10含量增高,IL-1β、IL-6和IL-18含量降低,TNF-α、TNFR1和IL-1β mRNA表达降低,RhoA和ROCKⅡ蛋白表达降低,差异均有统计学意义(P<0.05)。结论 NBP能够减轻EAE小鼠外周及中枢炎症反应,进而改善EAE的神经障碍症状,其作用机制可能是通过抑制RhoA/ROCK信号通路来实现的。 展开更多
关键词 L-3-正丁苯酞 多发性硬化 自身免疫性脑脊髓炎 RhoA/ROCK信号通路 神经炎症 C57BL/6小鼠
下载PDF
Effect of Seismic Permanent Deformation on Safety and Stability of Earth-Rock Dam Slope
14
作者 戚蓝 陈启振 蔡建成 《Transactions of Tianjin University》 EI CAS 2015年第2期167-171,共5页
In order to study the effect of seismic permanent deformation on the safety and stability of earth-rock dam, the permanent deformation is considered as the non-design permanent load, and the stress-strain hysteresis c... In order to study the effect of seismic permanent deformation on the safety and stability of earth-rock dam, the permanent deformation is considered as the non-design permanent load, and the stress-strain hysteresis curve is also considered when the earth is under cyclic load. The research work can make the calculation results of plastic collapse more accurate by including the effect of the post-earthquake degree of plastic deformation on the stability of the earth-rock dam, and the dam safety factor decreases from 2.50 to 1.90 after the magnitude-8 earthquake. Moreover, the research work will also improve the design of the earth-rock dam under abnormal operating conditions. 展开更多
关键词 EARTHQUAKE PERMANENT DEFORMATION earth-rock DAM safety FACTOR sensitivity analysis
下载PDF
Dynamic Risk Assessment Index System for Earth-Rock Dam during Construction
15
作者 葛巍 李宗坤 +1 位作者 李巍 赵梦蝶 《Journal of Donghua University(English Edition)》 EI CAS 2015年第6期923-926,共4页
During the construction of earth-rock dam,mutual exclusive construction goals such as quality,safety,progress and cost all have influences on each other,with risk factors being everchanging as the construction progres... During the construction of earth-rock dam,mutual exclusive construction goals such as quality,safety,progress and cost all have influences on each other,with risk factors being everchanging as the construction progresses.Accurate identification of the risk factors,as well as clearing any possible effect that any risk factors might have on the construction project is the key and foundation to our cooperative control of the construction goals.According to the construction planning of earth-rock dam,the hall3 D structure was used to identify the potential changes of risk factors,and the possible means of any risk factors to interfere with the construction goals.The dynamic risk assessment index system is established by deploying the WBS-RBS(work breakdown structurerisk breakdown structure)method,aiming at the construction goals of earth-rock dam.The case study shows that the index system is very effective at risk management of earth-rock dam during construction,and relatively practicable. 展开更多
关键词 hydraulic structure construction of earth-rock dam assessment index risk path construction goal
下载PDF
Study on life-cycle risk management of high earth-rock dam project
16
作者 Zhang Nianmu Zhang Zongliang Yan Lei 《Engineering Sciences》 EI 2013年第5期45-50,共6页
Based on advanced computer technology,internet of things(IOT) technology,project management concept and professional technology and combined with the innovative theories,methods and techniques in earlier hydropower pr... Based on advanced computer technology,internet of things(IOT) technology,project management concept and professional technology and combined with the innovative theories,methods and techniques in earlier hydropower projects,the life- cycle risk management system of high earth- rock dam project for Nuozhadu project was developed. The system mainly includes digital dam,three-dimensional design,construction quality monitoring,safety assessment and warning,etc,to integrally manage and analyze the dam design,constructional quality and safety monitoring information. It realized the dynamic updates of the comprehensive information and the safety quality monitoring in the project life cycle,and provided the basic platform for the scientific management of the construction and operation safety of high earth- rock dam. Application in Nuozhadu earth- rock dam showed that construction safety monitoring and warning greatly helped accelerate the construction progress and improve project quality,and provided a new way for the quality safety control of high earth-rock dam. 展开更多
关键词 风险管理系统 项目生命周期 土石坝工程 工程施工质量 计算机技术 安全评估 质量安全控制 三维设计
下载PDF
miR-153-3p靶向下调Rho相关螺旋蛋白激酶1(ROCK1)基因抑制乳腺癌细胞增殖及迁移 被引量:7
17
作者 孙蕾 王华 +1 位作者 姜珏 毕学苑 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2020年第2期138-144,共7页
目的探究miR-153-3p调节乳腺癌细胞的增殖、迁移及侵袭的分子机制。方法利用生物信息学软件检索预测miR-153-3p的候选靶基因;采用双荧光素酶报告实验分析miR-153-3p与候选基因的靶向关系;应用实时荧光定量PCR和Western blot法检测miR-15... 目的探究miR-153-3p调节乳腺癌细胞的增殖、迁移及侵袭的分子机制。方法利用生物信息学软件检索预测miR-153-3p的候选靶基因;采用双荧光素酶报告实验分析miR-153-3p与候选基因的靶向关系;应用实时荧光定量PCR和Western blot法检测miR-153-3p对MDA-MB-231细胞mRNA和蛋白质表达水平的影响;通过噻唑蓝(MTT)法检测细胞增殖能力,划痕实验检测细胞迁移能力,并以TranswellTM检测细胞的迁移和侵袭能力。结果软件预测显示miR-153-3p与Rho相关螺旋蛋白激酶1(ROCK1)有连续的结合区域;共转染miR-153-3p模拟物(miR-153-3p mimics)与ROCK1野生型报告载体的细胞相对荧光素酶活性降低;转染hsa-miR-153-3p-mimics后,MDA-MB-231细胞的miR-153-3p表达水平显著增高,且ROCK1蛋白的表达量明显降低。与对照组相比,转染组MDA-MB-231细胞的增殖、迁移和侵袭能力均减弱。结论miR-153-3p通过靶向下调ROCK1的表达来抑制乳腺癌细胞的增殖、迁移和侵袭。 展开更多
关键词 乳腺癌细胞 miR-153-3p Rho相关螺旋蛋白激酶1(ROCK1) 细胞增殖 细胞迁移
下载PDF
内皮素-1活化ROCK/SLUG诱导人卵巢癌细胞发生上皮向间充质转分化 被引量:2
18
作者 张阿丽 张弓 +5 位作者 彭晋 王全胜 马欢 钟亚华 周福祥 谢丛华 《中国药理学通报》 CAS CSCD 北大核心 2012年第5期680-686,共7页
目的研究ROCK/SLUG信号通路在内皮素-1(endo-thelin-1,ET-1)促进人卵巢癌细胞上皮向间充质转分化(epi-thelial to mesenchymal transition,EMT)中的作用。方法 ET-1处理人卵巢癌细胞株SK-OV-3和CaOV3,或共用ROCK的活化突变体转染细胞或... 目的研究ROCK/SLUG信号通路在内皮素-1(endo-thelin-1,ET-1)促进人卵巢癌细胞上皮向间充质转分化(epi-thelial to mesenchymal transition,EMT)中的作用。方法 ET-1处理人卵巢癌细胞株SK-OV-3和CaOV3,或共用ROCK的活化突变体转染细胞或加入ROCK的抑制剂Y27632,并转染含SLUG启动子的pGL3质粒与Renilla质粒。实验末用Boyden小室体外侵袭实验检测细胞侵袭能力,细胞免疫荧光染色观察细胞形态,报告基因检测试剂盒检测SLUG启动子活性,用实时定量PCR和Western bot方法检测EMT相关基因的表达。结果 ET-1诱导SK-OV-3和CaOV3发生与EMT相一致的形态和基因变化,促进其细胞侵袭力;ET-1与内皮素A受体(endothelin A receptor,ETAR)结合,促进转录因子SLUG的转录;ET-1促进ROCK及fibronectin的表达,同时转染ROCK的活化突变体,促进ET-1诱导的fibronectin表达以及细胞侵袭力的增加。相反,ROCK抑制剂Y27632抑制ET-1对fibronectin表达以及细胞侵袭力的促进作用;转染ROCK的活化突变体,上调SLUG基因转录启动子活性促进其转录,抑制E-cadherin的转录。相反,ROCK的抑制剂Y27632抑制SLUG基因启动子的活性。结论 ET-1通过活化ROCK/SLUG通路促进人卵巢癌细胞发生EMT。 展开更多
关键词 内皮素-1 内皮素A受体 上皮向间充质转分化 人卵巢癌 ROCK SLUG
下载PDF
丹参酚酸B盐抑制大鼠肝星状细胞中内皮素-1激活的RhoA/ROCK Ⅱ信号通路 被引量:2
19
作者 徐虹 平键 +2 位作者 卢超 周扬 徐列明 《肝脏》 2014年第4期261-265,共5页
目的探讨丹参酚酸B盐(Sal B)对大鼠肝星状细胞(HSC)中内皮素-1(ET-1)激活的RhoA/ROCK信号通路的影响。方法采用肝脏原位灌流酶消化、Nycodenz密度梯度离心法分离大鼠HSC。免疫蛋白印迹法检测肌球蛋白磷酸酶靶亚基1(MYPT1)磷酸化。特异... 目的探讨丹参酚酸B盐(Sal B)对大鼠肝星状细胞(HSC)中内皮素-1(ET-1)激活的RhoA/ROCK信号通路的影响。方法采用肝脏原位灌流酶消化、Nycodenz密度梯度离心法分离大鼠HSC。免疫蛋白印迹法检测肌球蛋白磷酸酶靶亚基1(MYPT1)磷酸化。特异性抗体沉淀ROCK,进行体外磷酸化反应,以磷酸化底物Thr696-MYPT1(654-880)的含量反映ROCK活性。GST下拉实验检测RhoA活性。结果在大鼠HSC中,ET-1刺激后,RhoA和ROCK Ⅱ活性显著增加,ROCK I活性无明显变化,MYPT1 Thr696和Thr850磷酸化水平均显著增加。ET-1刺激1 min和10 min时,RhoA活性分别是基础状态下的1.95倍(P<0.05)和5.84倍(P<0.01)。ET-1刺激2.5 min和15 min时,ROCK Ⅱ活性分别是基础状态下的3.49倍和4.83倍,均P<0.01,差异有统计学意义。ET-1刺激2.5 min时,MYPT1 Thr696磷酸化水平是基础状态下的3.86倍;刺激15 min时,Thr696磷酸化达到高峰,是基础状态下的5.17倍。Thr850磷酸化亦在ET-1刺激15 min达到高峰,是基础状态下的3.33倍,均P<0.01,差异有统计学意义。在ET-1刺激前给予10-5 mol/L Sal B预处理,则使ET-1诱导的RhoA和ROCK Ⅱ活性分别下降66.84%和76.79%,ET-1诱导的MYPT1 Thr696磷酸化下降80.09%,对Thr850磷酸化水平无影响。结论 Sal B能显著抑制大鼠HSC中ET-1诱导的RhoA和ROCK Ⅱ活化,抑制MYPT1Thr696磷酸化。 展开更多
关键词 肝星状细胞 内皮素-1 丹参酚酸B RHOA ROCK信号通路
下载PDF
基于FOA-RF算法的心墙砾石土压实质量预测模型 被引量:1
20
作者 崔博 闫辰博 王佳俊 《水利水电科技进展》 CSCD 北大核心 2023年第3期42-48,共7页
针对随机森林(RF)算法预测心墙砾石土压实质量存在决策树数量选取缺乏深入研究和忽视P0.075质量分数对压实质量影响的问题,提出了一种基于果蝇优化(FOA)算法的随机森林算法(FOA-RF算法),并构建了基于FOA-RF算法的心墙砾石土压实质量预... 针对随机森林(RF)算法预测心墙砾石土压实质量存在决策树数量选取缺乏深入研究和忽视P0.075质量分数对压实质量影响的问题,提出了一种基于果蝇优化(FOA)算法的随机森林算法(FOA-RF算法),并构建了基于FOA-RF算法的心墙砾石土压实质量预测模型(FOA-RF模型)。该模型一方面通过对料源参数和干密度进行相关性分析,新增了P0.075质量分数作为模型的输入参数;另一方面利用FOA算法对随机森林进行优化,解决了RF算法难以取得决策树数量最优解、没有同时考虑决策树数量与随机特征数影响的问题。以西南某在建砾石土心墙堆石坝工程为例,分别应用基于传统RF、BP神经网络、多元线性回归的预测模型和FOA-RF模型进行压实质量预测。结果表明,FOA-RF模型在预测精度上具有优越性,并基于该模型开发压实质量预测模块,将该模块嵌入碾压质量实时监控系统中可实现压实质量的实时预测。 展开更多
关键词 堆石坝 砾石土心墙 压实质量预测 随机森林 果蝇优化算法
下载PDF
上一页 1 2 113 下一页 到第
使用帮助 返回顶部